Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
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APPLICATION OF BROMELAIN LITTER SOLID COMPOST INDUCED BY LIGNINOLITIK Trichoderma sp. FUNGUS TOWARDS NUMBER OF LEAVES AND CHLOROPHYL CONTENT CHILI PLANTS (Capsicum annuum L.)
Full text linkPiles of uncontrolled pineapple peel waste have a negative impact that will affect various aspects of life, either directly or indirectly. Therefore, pineapple peel waste that can no longer be processed can be used for making compost. The taste of pineapple can be degraded biologically with the help of enzymes that can be produced by microorganisms. One of the microorganisms that can support the decomposition process is a group of fungi. In this study, the fungus Trichoderma sp. (Bioggp 5) is a ligninolytic fungus. Trichoderma sp. is a saprophytic soil microorganism that attacks natural pathogenic fungi and is beneficial to plants. The purpose of this study was to determine the effect of solid bromelain compost inoculum of ligninolytic fungi (Trichoderma sp.) on leaf number and chlorophyll content of chili plants (Capsicum annum L.) and the best dose of compost that could be used. This study used a completely randomized design (CRD) with 7 treatments, namely T0 (control), T1 (1.4% pure bromelain), T2 (1.7% pure bromelain), T3 (2% pure bromelain), T4 (1, 4% bromelain + leaf litter), T5 (1.7% bromelain + leaf litter), T6 (2% bromelain + leaf litter). The parameters used were the number of leaves and chlorophyll content. And the results obtained from this study were the T3 dose (2% pure bromelain) was the best dose for the number of leaves, while the T2 dose (1.7% pure bromelain) was the best dose for chlorophyll content
ANATOMIC CHARACTERISTICS OF THE STEM OF MANGO (Mangifera spp.) IN BANDAR LAMPUNG
Full text linkMango is one of the most widely grown plants in Indonesia. The characteristic that distinguishes one type of mango from another is to observe its anatomy. Anatomical research on the mango branch has not been done much, especially in Bandar Lampung. The purpose of this study was to determine the diameter and pith radius of the mango (Mangifera spp.) branch in Bandar Lampung City. This research was carried out from January to April 2021 at the Botanical Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung. This study uses a survey method with Simple Random Sampling in 20 sub-districts in Bandar Lampung City. Parameters measured included tracheal diameter, tracheal density, arrangement and type of pith radius, pith radius height, and pith radius width. Data analysis used descriptive analysis by explaining the data obtained in the form of tables and photos. The results showed that there were differences in the average size of the tracheal diameter, tracheal density, height, and width of pith radius of the five mango branch samples. The average diameter of the largest trachea is found in Golek and the smallest tracheal diameter is found in Kweni. The highest average tracheal density is found in Arumanis and the smallest tracheal density is found in Golek. The arrangement and type of pith radius of the fifth mango are uniseriate and heterocellular. The average height of the largest pith radius is owned by Indramayu and the smallest is owned by Golek. While the average width of the largest pith radius is owned by Golek and the smallest is owned by Gedong.
MANUFACTURE OF LIGNINOLYTIC FUNGI INOCULUM Geotrichum sp. WITH SORGUM (Sorghum bicolor) MEDIA AND ITS EFFECT ON THE QUALITY OF BAMBOO LEAF COMPOST (Bambusa sp.)
Full text linkBamboo is a type of plant that grows in Indonesia. So far, people only use the stems and throw away the leaves. As a result, these abundant bamboo leaves become waste. Bamboo leaf waste contains lignin content of 19.8-26.6%, and becomes compost in the long term. The process of composting bamboo leaf litter can be accelerated using an activator such as the inoculum fungi Geotrichum sp. which is ligninolytic so that it can break down lignin complex compounds contained in bamboo leaf waste into simpler compounds. Sorghum contains about 6.5-7.9% fiber. The high fiber content in sorghum seeds can be used as a growth medium for fungal inoculum. The purpose of this study was to determine the effect of sorghum as an inoculum medium for Geotrichum sp. and the effect of inoculum on compost quality. This research was carried out in August - December 2019 at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, Unila. This study used a completely randomized design (CRD) method with 4 treatments, namely Control (Bamboo leaf litter), P1 (Bamboo leaf litter + 1% inoculum), P2 (Bamboo leaf litter + 1.5 % inoculum) and P3 (Bamboo leaf litter). + 2% inoculum) each treatment was repeated 3 times. The variables observed were the number of spores and spore viability using the Colony Forming Unit (CFU) calculation on inoculum and compost. Analysis of the content of C, N, P, and C/N ratio was carried out to determine the quality of the compost. Based on the research results, sorghum can be used as a growth medium for the ligninolytic fungi inoculum Geotrichum sp. with spore viability of 74.18% and the addition of 1.5% inoculum (P2) gave the best results on the quality of bamboo leaf compost (Bambusa sp.
DETECTION OF NITROGENASE PRODUCING BACTERIA FROM THE SOIL OF LIWA BOTANICAL GARDEN
Full text linkLiwa Botanical Gardens is an ex-situ conservation area for various types of plants. Each plant produces organic matter that will provide nutrients for the growth of nitrogen-fixing bacteria. This indicates the existence of an environment that supports the growth of nitrogen-fixing bacteria. Nitrogen is one of the nutrients needed by plants for their growth. However, the abundance of nitrogen in the atmosphere cannot be utilized directly by plants but needs to transform into ammonium and nitrate first. This transformation can be done by nitrogen-fixing bacteria through an enzymatic process. This research aims to obtain bacterial isolates that can fix nitrogen. Nitrogen-fixing bacteria were isolated using Nutrient Agar (NA) medium and furthered by nitrogenase activity detection test with semi-solid Nitrogen Free Bromothymol Blue (NFB). Nitrogen-fixing bacteria are characterized by color changes in the medium. The results obtained 22 isolates with 3 isolates detected capable of producing nitrogenase enzymes, namely TBP B3, TB1 B2, and TMA2 B2
The Diversity of Grasshoppers in Liwa Botanical Garden Based on Time Capture
Full text linkGrasshopper is one of the diurnal cosmopolitan insect, the existence of grasshoppers is very influential for some other animal populations such as birds. It is still lack of information of diversity, distribution, population, and other basic biological aspects of grasshoppers at Kebun Raya Liwa (KRL). To monitor biodiversity, it is necessary to provide informations about the number of individuals, their functions, and roles in the habitat and ecosystem. Therefore it is necessary to conduct the research of these topics. This research was carried out during November to December 2019, at the Taman Hias Kebun Raya Liwa. Grasshoppers were cached in the morning from 06.00 AM to 09.00 AM, day from 10.00 AM to 02.00 PM, and afternoon from 03.00 PM to 06.00 PM . The grasshoppers was cached using a sweeping net by exploring the Taman Hias Kebun Raya Liwa. The caught grasshopper was identified at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Universitas Lampung. The result show that ten species of grasshopper were found at the Taman Hias Kebun Raya Liwa, i.e. Phaleoba antennata, Phaleoba fumosa, Phaleoba rustica, Atractomorpha crenulata, Atractomorpha sp., Phillium crurifolium, Phillium sp, Trilophidia annulata, Oxya chienensis, and Melanoplus differentialentialis. The index diversity of grasshopper at the Taman Hias Kebun Raya Liwa is categorized to moderate with H' = 2.223. The best time to catch grasshoppers is in the morning from 06.00 AM-09.00 AM and afternoon from 03:00 PM – 05:00 PM
THE EFFECT OF POLYETHYLENE GLYCOL (PEG) 6000 ON GREEN MUSTARD (Brassica juncea L.) GERMINATION AND GROWTH
Full text linkThe frequent drought in Indonesia has caused many farmers to experience a decline in vegetable production, one of which is green mustard. The green mustard is not resistant to drought, so water supply is an aspect that must be given special attention in cultivating green mustard. The objective of this study was to know whether the seedling and growth of seedling of green mustard (Brassica juncea L.) can grow well under drought stress by Polyethylene glycol 6000. This study was conducted in the Laboratory of Plant Physiology, Department of Biology, Faculty of Mathematics and Natural Science, the University of Lampung in 2019. This study was conducted in a Completely Randomized Design with the main factor is Polyethylene glycol 6000 with three levels: 0% w/v, 10% w/v, 20% w/v, and 30% w/v. Each combination of treatments was repeated five times. The number of the experimental unit was 20. The observed variables in this study were the percentage of seed germination, length of leaf, the width of leaf, a,b chlorophyll content, and total chlorophyll content. The data was homogenize using the Levene test, then continued to analyze using ANOVA at a 5% significant level and proceed with HSD test significant level 5% to determine the differences between the treatment. The result showed that under drought stress by Polyethylene glycol 6000 started to affect green mustard seedling and growth of seedling at the level of PEG 6000 concentration 20% w/v while the number of leaves not affected. The conclusion was PEG 6000 gave a negative impact on the green mustard and the green mustard induced by PEG 6000 10% w/v more adaptive than PEG 6000 20 w/v
Morphological Characteristics of Araceae Plants in Liwa Botanical Garden, West Lampung
Full text linkAraceae is one type of plant that has a fairly high level of diversity and also rich in benefits such as a source of food, ornamental plants, and medicine. Research on the characteristics of Araceae in Indonesia is still limited. The purpose of this study was to identify Araceaeplants based on morphological structure and analyze the morphological characteristics ofAraceae in Liwa Botanical Garden, West Lampung. This research was conducted from November 2019 to January 2020. Data were collected at Liwa Botanical Garden, WestLampung based on exploration and observation collection methods. Morphological structure data were analyzed as descriptive and qualitative. The parts of plants that are compared between Araceae plants are the shape, size of leaves, petiole, and inflorescences. Observations show that 21 genera of Araceae are consisting of 26 plants. Araceae plants have three different ways of life, namely terrestrial, epiphytic, and aquatic. The shape of the leaves is ovatus, cordatus, lanceolatus, cuneatus, needle, peltatus, sagittatus, hastatus, palmatisect, bipinnatisect and tripinnatisect. The characteristics of this plant have compound interest with cob types (spadix) which are covered by a spatha with two types of inflorescences, namely unisexual and bisexual
Normality of The Mice's Fetal Spine during Fennel Flower Extract (Nigella sativa) Feeding
Full text linkBird Species Diversity in Liwa Botanical Garden, West Lampung
Full text linkoai:jbekh.jurnalbiologi.fmipa.unila.ac.id:article/5The diversity of bird species can reflect the high biodiversity of an area. it means that birds can be used as a bio-indicator of environmental quality. Determining the quality and level ofenvironmental damage can be seen from the diversity of bird species in the region. Environmental quality will be better if the diversity of bird species in the region is diverse.Birds have an important role in protecting the ecosystem in the Liwa Botanical Garden. The presence of birds in the Liwa Botanical Garden has not been well identified. Therefore thisresearch needs to be done. This research was conducted on 12 October 2019 to 20 October 2019 in the Liwa Botanical Garden, West Lampung. Data collection of bird species isconducted in the morning at 06.30.00-08.30 West Indonesia Time and afternoon at 16.30-18.00 West Indonesia Time, with a combination of the count point and the transect method then all encounters with birds are recorded and identified directly at the study site. After the birds are identified, data is processed to find out Shannon's level index and diversity index. The species diversity index uses the Shannon and Weiner formula. The level of diversity is determined based on the species diversity index. The results showed that there were 15 bird species with a total of 466 individuals with a moderate diversity index and an evenness index indicating a distressed community.
Birdwatching Potency at Liwa Botanical Garden, West Lampung
Full text linkThe Liwa Botanical Garden is located in Kubu Perahu Village, Balik Bukit District, West Lampung Regency. The Liwa Botanical Garden has an area of 86 ha, build in 2007 with the theme of Indonesian Ornamental Plants. The Liwa Botanical Garden is one of the destinations that is being developed for tourism activities and used as a recreational object. One of the functions of the Liwa Botanical Garden is as a tourist spot. Plants in the Liwa Botanical Garden are so beautiful. The Liwa Botanical Garden is a bird habitat because it has an abundant source of food for birds. Therefore it is necessary to do an inventory of bird species by conducting research to find out potential bird species that are used as tourist attractions (birdwatching) at the Liwa Botanical Garden. This research was conducted on October 2019. The method used was the point count method and rapid assessment. Based on field observations found 14 species of birds that are not protected and only 1 species of birds included in the protected category. And found 8 types of plants, namely 5 types of trees, 1 species of ferns, 1 species of bushes, and 1 type of reeds