GreenPrints Institutional repository of De La Salle Medical and Health Sciences Institut
Not a member yet
6526 research outputs found
Sort by
Primary surveillance of blages-positive extended-spectrum beta-lactamases-producing Escherichia coli (ESBL-E coli) in treated sewage water from a selected tertiary hospital in Dasmariñas, Cavite
No full textExtended-Spectrum Beta-Lactamase-Producing Escherichia coli (ESBL-producing E. coli) and its ability to survive in wastewater poses a great concern to the environment. This study shows the primary surveillance of ESBL-producing E. coli in a tertiary hospital’s water treatment plant. Multiple-tube fermentation technique was used in the determination of MPN while real-time polymerase chain reaction was utilized to confirm the presence of the blaGES gene in the isolated E. coli. Samples were collected in fifteen replicates for each month. The data gathered showed that all monthly samples showed an MPN of \u3e8.0/100mL. All samples also demonstrated a negative result in RTPCR for the blaGES gene, signifying that no blaGES-positive ESBL-producing E. coli was observed during the three-month surveillance period
Hemagglutination activity of semi-purified lectin from Dioscorea alata (Ube)
No full textSpecies from the Dioscoreaceae family have been studied for their clinical implications, including blood typing. Studies have indicated the presence of lectins in Dioscorea spp., including Dioscorea alata, which suggests potential for hemagglutination research. To obtain the semi-purified lectin extract, crude lectin was extracted using phosphate-buffered saline. Ammonium sulfate fractionation was utilized for the semi-purification of the crude lectin extract, and different dialysate concentrations were prepared. Total protein content determination was performed for each dialysate to confirm the presence of protein. Only A and B blood groups were included. Selected participants underwent proper ethical considerations for their participation. To determine the hemagglutination activity of the lectins, 96-well U-shaped microtiter plates were utilized. Serial dilutions were performed up to the 24th well for each dialysate concentration to determine the hemagglutinating titer. Red cell suspensions were also prepared from the samples collected from each participant. The lectin dialysates of each concentration were able to exhibit hemagglutinating activities in an increasing trend with the 90% dialysate achieving the highest hemagglutinating titers for blood groups A and B
A cross-sectional study on the molecular detection of multidrug resistant bacteria in bus air conditioners along the Parañaque Integrated Terminal Exchange (PITX) to Indang, Cavite Route
No full textThis cross-sectional study aims to determine if multidrug-resistant bacteria are present in the air conditioners of buses traveling along the PITX-Indang, Cavite route, and to establish the correlation between cleaning frequency and the presence of Gram-negative bacteria. Samples were collected from the vents of bus air conditioners using a swab method on tryptic soy broth, while data on bus cleaning frequency were acquired through interviews with responsible air conditioner technicians. A total of 15 buses had been swabbed, but only 11 showed growth upon serial dilution on MacConkey agar plates. These samples and data were then subjected to one-time quantitative PCR and descriptive statistical treatment. The molecular detection, specifically targeting the blaGES genes, revealed that there were no multidrug-resistant bacteria in the bus air conditioners; however, the swab sample contained a number of gram-negative bacteria. Upon undergoing gel electrophoresis, all samples showed a band pattern. However, 13 samples resulted in a negative outcome, and 1 sample showed a result caused by contamination under molecular detection. Gram-negative bacteria found in the samples can be linked to bus companies\u27 inconsistent and inefficient cleaning procedures. Even if there were no multidrug-resistant bacteria on the bus air conditioners, gram-negative bacteria might still be harmful to the health of both passengers and staff. In order to eradicate their presence and lower the potential risk to commuters, frequent cleanliness is thus advised
Effects of living away from home on the academic performance of the Senior High School Grade 11 students of the De La Salle Medical And Health Sciences Institute A.Y. 2023-2024
No full textKnowledge, attitudes, and practices towards the use of recovery strategies in sports among collegiate coaches
No full textRecovery strategies are used in conjunction with physical training to achieve an athlete’s peak performance. Coaches play a vital role in advocating and implementing these techniques. However, there were noted inconsistencies in its application due to gaps in knowledge or heavy reliance on practical experience which affects the coaches’ perception and utilization of these techniques. With that, there is a need to establish the need to strengthen their knowledge on different recovery methods, their attitudes towards its use, and its integration in their coaching routines. A quantitative descriptive study was conducted to 31 collegiate coaches in Dasmariñas City, Cavite using a self- made paper-based questionnaire to assess their knowledge, attitudes, and practices (KAP) towards recovery strategies. Results showed that the coaches demonstrated a high level of knowledge on recovery, foster highly positive attitudes, and use recovery strategies to a very great extent. Comparison of means showed no significant difference when the coaches’ KAP were compared based on their sociodemographic characteristics (i.e., gender, sports handled, coaching experience, educational background). However, a statistically significant difference was noted when their knowledge on recovery was compared to their age, indicating its influence on how coaches understand concepts in recovery. The study highlights that while formal education is a major influence, continuous professional development, curiosity about new trends, and personal motivation also plays a significant role in improving the attitude and practices of coaches. Thus, the study suggests a need for further education and training programs to bridge the gap and promote evidence- based practices
Predictors of academic performance in human anatomy and physiology subjects of first- and second-year physical therapy students in De La Salle Medical and Health Sciences Institute
No full textHuman Anatomy and Physiology are subjects for health-allied students as they cover the foundation, concepts, and skills for their future profession. The course requires active learning from the students and to achieve academic success, students need an extensive understanding leading to clinical proficiency. This study aims to identify predictors of academic performance in human anatomy and physiology subjects among first- and second-year students of De La Salle Medical and Health Sciences Institute from the Academic Year 2024-2025. The study used a quantitative, non-experimental, and cross-sectional design. A total of 166 first- and second-year physical therapy students of DLSMHSI from A.Y. 2024-2025 participated in the study, selected through purposive sampling. Data were collected via room-to-room orientations through printed self- made questionnaires containing socio-academic information, perceived difficulty in organ systems, and standardized questionnaires examining motivation, stress, and learning strategies. Manual electronic coding of the collected data and final raw grades were done by a hired independent data manager. The SPSS software was used for descriptive and multivariate regression analysis. Results indicate that test anxiety is the sole predictor of academic performance, and for every unit increase in test anxiety, there is a 40% decrease in the likelihood of obtaining a higher academic performance. The study highlights that motivation, specifically test anxiety, is a predictor of academic performance among students who took human anatomy and physiology subjects. Further strategies can be explored to address students’ test anxiety and stress levels
In vitro evaluation of the anticoagulant and thrombolytic properties of Ehretia microphylla (tsaang gubat) leaves extract
No full textThe study aimed to evaluate the in vitro anticoagulant and thrombolytic properties of Ehretia microphylla (tsaang gubat) ethanolic leaf extract using human blood samples. The extract was subjected to phytochemical screening and tested for its ability to prevent and dissolve blood clots through the assessment of Prothrombin Time (PT), Activated Partial Thromboplastin Time (aPTT), and clot lysis. Flavonoids, tannins, alkaloids, phenols, and saponins were identified in the extract. These are phytochemicals associated with anticoagulant and thrombolytic activities. Three concentrations of the extract (5%, 10%, and 20%) were tested for the anticoagulant activity, while another set of three concentrations (50%, 75%, and 100%) was used for thrombolytic testing. Results demonstrated a dose-dependent effect, with 20% concentration exhibiting the highest anticoagulant activity and the 100% concentration yielding the greatest clot lysis. The anticoagulant effect was evaluated against heparin (positive control), while thrombolytic activity was compared with streptokinase (positive control). Statistical analysis revealed significant differences between the effects of E. microphylla and the positive controls, suggesting that the extract possesses notable anticoagulant and thrombolytic activities. These findings support the potential use of E. microphylla as a natural therapeutic agent for thrombotic disorders
Community health research project Block 20 and 24, Brgy. Fatima II, Dasmariñas City, Cavite
No full textJewelry egg box 5
No full text1819-RPA-068https://greenprints.dlshsi.edu.ph/collections_3d/1009/thumbnail.jp