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Keepunumuk: Teaching the Deconstruction of the “Myth of the First Thanksgiving”
The goal of this project was to volunteer to teach a reading activity for elementary school students about the indigenous peoples (Wampanoag) contact in 1621 from the indigenous perspective and deconstruct the “First Thanksgiving myth” using the book “Keepunumuk: Weeâchumun\u27s Thanksgiving Story” published on August 2, 2022, by authors Danielle Greendeer, Anthony Perry, and Alexis Bunten
Isolating and Characterizing novel Veillonella Phages and Phage-Resistant Mutants
Bacteriophages (phages) are viruses that target bacteria. Lytic phage infections cause cell destruction, while lysogenic phages integrate into the bacterial genome and excise under certain conditions to become lytic. Phage therapy is of growing interest as a possible alternative or enhancement to antibiotic treatment. Veillonella parvula is a common oral microbe and has been found to aid the colonization of Porphyromonas gingivalis, a major cause of gum disease. Very few phages have been identified for V. parvula and other Veillonella species and none have been characterized or sequenced
Visualizing and Analyzing the Growth of Dark Septate Fungi Within Plant Roots
Plants rely heavily on relationships between plant roots and root associated symbionts like bacteria and fungi Arbuscular mycorrhizal fungi (AMF) can help plants take up nutrients and limit the uptake of heavy metals Previous AMF experimentation in lab led to observation of high quantities of dark septate fungi (DSF) in cold stored roots DSF are far less researched than AMF; have melanized and septate hyphae, found to colonize roots worldwide, but growth patterns and potential functions are not yet understoo
Impacts of culture conditions for Rhizobium rhizogenes-mediated hairy root transformation of tomato
Rhizobium rhizogenes is a plant pathogen that is used to generate transgenic “hairy roots” in a variety of plant species. However, it was observed that repeated freeze-thaw cycles decreased transformation efficiency. Therefore, the aim of this experiment was to determine if transformation efficiency could be recovered by re-culturing the bacteria. Rhizogenes was thawed either 1 or 5 times and then regrown using either solid or liquid media. We found that freeze-thawing alone was not enough to decrease transformation efficiency, as the 1 thaw and 5 thaw treatments both yielded a high number of transformed plants. Future work will investigate other factors, such as increased time and severity of thaw on transformation efficiency