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Analysis of intestinal flora and environmental microbial diversity of Takifugu rubripes
Get PDFTakifugu rubripes (T. rubripes) is marine fish rich in protein and essential amino acids. With the continuous development of T. rubripes farming, intensive aquaculture has increased the infection rate with fish diseases. To explore the relationship between environmental microbial communities and gut microbiota, we sequenced the 16s rRNA gene V3–V4 region of the microorganisms in the aquaculture water and gut flora of T. rubripes. The results indicated 934 operational taxonomic units for T. rubripes gut contents and aquaculture samples. A total of 31 phyla and 498 genera were identified. At the phylum level, except for the phylum Proteobacteria, the dominant phyla in intestinal contents were Firmicutes, Acidobacteria, and Fusobacteria. However, the dominant phyla in water were Actinobacteria, Cyanobacteria, and Patescibacteria. At the genus level, intestinal contents included Photobacterium, Arcobacter, Vibrio, and Ruminococcaceae. The water samples mainly included CladeIa, Rhodobacteraceae, Eutreptiella pomquetensis, Lentibacter, CladeIII, and PeM15. Principal component analysis showed that the microbial compositions of samples from the same source were similar. There were significant differences between the intestinal flora and water microorganisms. Therefore, the research results showed the differences between the microbial communities in the intestinal tract and aquaculture water of T. rubripes and the characteristics of the main pathogenic bacteria; this could help guide the environmental regulation and disease prevention of T. rubripes aquaculture
Oral History Finding Aid: Robert "Lopaka" Kalani
Get PDFOral history finding aid of interviews with Robert "Lopaka" Kalani who was born in Pāʻia, Hamakuapoko, Maui in 1939. Interview footage and transcripts are available in the Hula Preservation Society library. For questions regarding materials referenced herein, and/or Access & Use of content, please contact them
Oral History Finding Aid: Marjorie Sumner
Get PDFOral history finding aid of interviews with Marjorie Kaahuopea Murray Sumner who was born in Honolulu, Kona, Oʻahu in 1930. Interview footage and transcripts are available in the Hula Preservation Society library. For questions regarding materials referenced herein, and/or Access & Use of content, please contact them
Registered Map 1737 : Fort Street, Kukui Street, Emma Street, Honolulu, Oahu
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Analysis of Chinese tilapia supply and demand without and with the COVID-19 epidemic impact
Get PDFWe analyzed the supply and demand for tilapia in China while assessing the future developmental trends. China has become the world’s largest producer, exporter, and consumer of tilapia. China entered a period of rapid aquaculture development in the 1990s, and the tilapia supply has increased yearly. Tilapia products are mainly supplied to the international market, especially the US. The global market for the Chinese tilapia has grown dramatically, but a downward trend occurred in 2019–2020. The Chinese domestic market demand is relatively stable, and even the COVID-19 epidemic did not significantly impact the supply and demand of tilapia. Internationally, it is expected that the demand for tilapia will decline considerably in the near future. However, this decline could be alleviated after the impact of the COVID-19 epidemic passes and increasing demand will resume. The increased supply of Chinese tilapia might slow down or even decrease due to market uncertainty, the increasing constraints on natural resources, and the Chinese government's requirements for high-quality aquaculture environments
Mitochondrial COI sequences revealed shallow but significant divergences among Amphioctopus aegina (Octopoda, Octopodidae) populations in coastal waters of China
Get PDFAmphioctopus aegina is an important fishery resource in the coastal waters of China. In the present study, the genetic diversity and population genetic structure among four populations of A. aegina throughout its distributional range in China were assessed using the mitochondrial cytochrome oxidase 1 (COI) sequences. The results revealed a generally low genetic diversity (Hd: 0.2842–0.6670; Pi: 0.0007–0.0015) in A. aegina populations. The neighbor-joining (NJ) phylogenetic tree and the haplotype networks, as well as the results of the molecular variance (AMOVA) analyses, indicated a shallow phylogeographic structure among the four populations. However, pairwise ΦST statistics and genetic distance analyses revealed significant (p<0.01) genetic differentiation among Qinzhou and the rest three populations of Zhanjiang, Huizhou, and Dongshan. The demographic history analyses indicated a population expansion in A. aegina, and the role of Leizhou peninsula isolation in shaping the population differentiation. These results would largely enhance our understanding of the genetic structure and hence promote the scientific management of A. aegina fishery resources in coastal waters of China
