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Improved reproductive performance of captive male broodstock of tiger shrimp Penaeus monodon with 17α-methyltestosterone induction
Get PDFThe purpose of this study was to evaluate the influence of different 17α-methyltestosterone injection frequencies at optimal doses to improve the quality of spermatophore and reproductive performance of captive male broodstock of tiger shrimp Penaeus monodon. Broodstocks of tiger shrimp weighing 51-65 g for males and 86-161 g for females were collected from ponds and stored in a 3,000 m3 concrete tank. Male broodstocks were injected with 17α-methyltestosterone at the optimized protocol at 300 ng/100 g body weight at different injection frequencies, three times, four times, and five times at seven-day intervals. As a control, male broodstocks were injected with a saline solution to determine the spermatophore quality. Evaluation of the reproductive performance of the captive male was observed in the captive female broodstock tiger shrimp using artificial insemination. The weight of spermatophore (0.041 g), gonadosomatic index (7.71%), the total number of spermatozoa (64 × 106 cells/mL) significantly increased, and the percentage of abnormal spermatozoa significantly decreased by 73% as well as hatching rate increased to 43.14% at the injection frequency of four times compared to the other treatments. In conclusion, injection of 17α-methyltestosterone at the dosage of 300 ng/100 g body weight at the frequency of giving four times was effective enough to improve the reproductive performance of captive tiger shrimp. Therefore, the method proposed can be a strategy to produce better larvae and increase the income of the aquaculture business
A study on the agglomeration characteristics of the scallop aquaculture industry in China
Get PDFScallops are economically important shellfish widely cultured in China. In this study, the agglomeration characteristics of the scallop aquaculture industry in Chinese coastal provinces were explored based on data from the China Fishery Statistical Yearbook from 2012 to 2020 and the comparative advantage and concentration level of the scallop breeding industry. The data were analyzed using the comprehensive relative advantage index, location entropy, and industrial concentration degree method. Two main results revealed that Hebei Province had the highest comparative advantage in terms of efficiency, followed by Fujian, Guangdong, Shandong, Guangxi, Liaoning, and Zhejiang Provinces. Hebei Province had the highest comparative advantage in scale, followed by Liaoning, Shandong, Hainan, Guangdong, Guangxi, Fujian, and Zhejiang Provinces. Additionally, the scallop cultivation industry in Hebei, Shandong, and Liaoning Provinces showed a high concentration, and the proportion of scallop production in the national production (CR4) in the three provinces, as well as in Guangdong, accounted for more than 99% year-round. Overall, Hebei, Shandong, and Liaoning Provinces had a comparative advantage in scale, comprehensive comparative advantage, and industrial agglomeration
Molecular cloning, expression pattern of Trypsin gene and association analysis with growth traits in Penaeus monodon
Get PDFA novel TRY homolog was cloned in Penaeus monodon by RACE technology, named PmTry (GenBank: KP998480). The PmTry cDNA was 916 bp, which encodes 266 amino acids with a predicted molecular weight of 28.38 KDa and an isoelectric point of 4.58. Homologous analysis indicated that PmTry shared 42%~91% similarity with other species. The phylogenetic tree showed that PmTry was closely related to Fenneropenaeus chinensis. Tissue expression profiles showed that PmTry was highest expressed in the hepatopancreas and the lowest expressed in the eyestalk nerve. It was expressed in the whole growth stage of P. monodon, but the relative expression level of each stage was significantly different. In addition, PmTry-524 and PmTry-798 were particularly related to growth traits of P. monodon by genotype. The SNP markers may provide a basis for genetic selection and breed improvement studies in P. monodon
Oral History Finding Aid: Kahauanu Lake
Get PDFOral history finding aid of interviews with Haywood Kahauanu Keolani Lake who was born in Wailuku, Pūʻali Komohana, Maui in 1932. Interview footage and transcripts are available in the Hula Preservation Society library. For questions regarding materials referenced herein, and/or Access & Use of content, please contact them
Oral History Finding Aid: George Naope
Get PDFOral history finding aid of interviews with George Lanakilakeikiahialii Naope who was born in Kalihi, Kona, O'ahu in 1928. Interview footage and transcripts are available in the Hula Preservation Society library. For questions regarding materials referenced herein, and/or Access & Use of content, please contact them
