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Bacillus velezensis LG37 absorbs and utilizes ammonia nitrogen from aquaculture water and enhances the toxicity tolerance of grass carp (Ctenopharyngodon idella) to ammonia (non-ionic ammonium)
Get PDFHigh concentrations of ammonia nitrogen lead to multi-organ damage, decreased immunity, and metabolic disorders in aquaculture animals, which cause disease outbreaks. This study mainly screened probiotic strains for good control of the concentration of ammonia nitrogen and identified strain LG37 as Bacillus velezensis according to its morphological, 16S rDNA, phylogenetic, and genomic data analysis. This LG37 strain achieved efficiently purifies aquaculture water and enhances the toxicity tolerance of grass carp to ammonia. The experimental on growth characteristics showed that LG37 exhibited a satisfactory growth trend with 10 types of sugar, 17 types of amino acids, and 6 types of organic nitrogen as the carbon or nitrogen source. Under the conditions of initial dissolved oxygen at 6±1 mg/L, the temperature at 27±2°C, and pH 7±0.3. Through wastewater treatment equipment, which degraded ammonia nitrogen and the feed protein in grass carp culture wastewater reached up to 55.5% and 73.6% within one week, respectively. Moreover, in the acute toxicity test, when feed without LG37 was fed to grass carp, the safe concentration of ammonia nitrogen (non-ionic ammonium) was 3.37 mg/L (0.2 mg/L). When feed with LG37 was fed to grass carp, the safe concentration of ammonia nitrogen (non-ionic ammonium) was 5.11 mg/L (0.31 mg/L). Compared to feed without LG37, the feed with LG37 increased the toxicity tolerance of grass carp to ammonia nitrogen (non-ionic ammonium) by 51.63% (55%)
Comparative study on body index, nutrient composition, and digestive enzyme activity of Misgurnus anguillicaudatus, Paramisgurnus dabryanus, and Paramisgurnus dabryanus ssp
Get PDFThe current research work was undertaken to compare and analyze the body index, nutrient composition, and digestive enzyme activity of Pond loach (Misgurnus anguillicaudatus), large-scale loach (Paramisgurnus dabryanus), and Taiwan loach (Paramisgurnus dabryanus ssp). Viscerosomatic ratio (VR), condition factor, (CF), W/L and H/L were highest in Taiwan loach (P < 0.05). Muscle protein content was highest, whereas lipid content was lowest in pond loach (P < 0.05). The content of total amino acids (TAA), total essential amino acids (EAA), and delicious amino acids (DAA) in the muscle of pond loach was highest (P < 0.05). The content of polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA) and linoleic acid was highest in pond loach, Taiwan loach and large-scale loach, respectively (P < 0.05). The trypsin activities and amylase activities of the pond loach were significantly higher than those of the large-scale loach and Taiwan loach in the intestine and liver (P < 0.05). These results indicate that the three kinds of loaches are of high nutritional value and have breeding prospects, among which pond loach has higher nutritional value
