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Effects of <em>Macleaya cordata</em> extract on intestinal microbiota of European eels (<em>Anguilla anguilla</em>) cultured in cement tanks
This trial investigated the effects of Macleaya cordata extract (MCE) on the diversity and composition of the intestinal microbiota of European eels (Anguilla anguilla) cultured in cement tanks. After routine grading eels, six cement tanks with similar body size and fish weight (about 167 g/fish and 3620 kg/tank) were randomly divided into the control and MCE groups. The control group was fed a commercial diet, and the MCE group was fed the same commercial diet supplemented with 100 mg/kg MCE. There were three tanks in each group. This trial lasted for six weeks. Compared with the control group, the European eels in the MCE groups had a higher richness and diversity of intestinal microbiota. At the phylum level, European eels in the MCE group had higher relative abundances of Firmicutes and Proteobacteria and lower relative abundance of Spirochaetae and Fusobacteria compared to those of the control group. At the genus level, the European eels in the control group had higher relative abundances of Streptococcus and Legionella. In comparison, the relative abundances of Bacillus, Anaerobacillus, and Sphingomonas were higher in the intestine of the European eels of the MCE group. In conclusion, the dietary 100 mg/kg MCE supplementation might beneficially change the intestinal microbiota of European eels by increasing the relative abundance of certain probiotics and inhibiting some pathogenic bacteria
Puka Puka Parade 2023 - Numbers 08 and 09
Double issue for both August and September 2023
The effect of dietary taurine and its potential biosynthesis on juvenile grey mullet (Mugil cephalus) performance
The grey mullet is a catadromous species that spawns in the taurine-rich seawater environment, followed by the young fish generally migrating to less saline, low taurine waters during the larva-juvenile transition. Consequently, this study aimed to (1) determines whether there is a dietary taurine requirement in juvenile grey mullet for enhanced growth and (2) the potential for taurine biosynthesis. The experimental system consisted of sixteen 400-l V-tanks, where filtered, UV-treated ambient seawater (40 ‰) entered the bottom of the tanks at a rate of 7 tank exchanges/day. This allowed the testing of four 1 mm pelleted diets (0, 0.5, 1.0, and 2.0% taurine DW diet) in replicates of 4 tanks/treatment for 58 days. Grey mullet juveniles demonstrated (P<0.05) a specific requirement for a 0.5% taurine DW diet for improved growth. Fish fed the taurine diets displayed populations with a markedly (P < 0.05) higher average number of surviving fish (23.4±1.1) of moderately sized (10- 20 g) cohorts than smaller (< 10 g) individuals (12.5±1.1). In contrast, the fish fed the taurine control (0% taurine) exhibited similar average numbers of small and moderate sized fish (18.0±3.6-20.0±4.1). Dietary taurine accumulated highly (P<0.05) in the muscles in a dose dependent manner but less so (P<0.05) in eyes, and liver. The gene expression of liver cysteine sulfinic acid decarboxylase (CSD) exhibited an upregulation (P<0.05) with taurine diets from 0 to 1% but was down regulated (P<0.05) in fish fed the 2% taurine DW diet
Papers from the 8th International Conference of Austroasiatic Linguistics
This volume contains eight articles from ICAAL 8 (the 8th International Conference of Austroasiatic Linguistics)
Bacillus cereus, a potential pathogen of snakehead fish Ophiocephalus argus
Bacillus cereus is an emerging pathogen that has caused high mortalities in aquaculture animals. Yet the pathogenicity of B. cereus in snakehead fish Ophiocephalus argus is still unclear. In this study, a virulent strain (CA4) was isolated from diseased snakehead fish suffering from a typical symptom of hepatic hemorrhage with blood vessel congestion and macrophage infiltration, and was identified molecularly and phenotypically as B. cereus. It was β-hemolytic, showed an LD50 value of 2.57×106 CFU mL-1 for snakehead fish, and developed multiple resistances to cotrimoxazole, doxycycline, florfenicol, neomycin, sulfisoxazole, and tetracycline in aquaculture use. To the best of our knowledge, this is the first report of snakehead fish- pathogenic B. cereus. The findings of this study provide new insights into the potential threat of pathogenic B. cereus to snakehead fish
Review of: The Brigands’ Song: Serving in the Army of a Native Chieftain - A Traditional Song Text from Guangxi in Southern China
This is a book review of "The Brigands’ Song: Serving in the Army of a Native Chieftain - A Traditional Song Text from Guangxi in Southern China.
Morphological characteristics and genetic diversity of <em>Terapon jarbua</em> (Forrskäl, 1775) in Central, Vietnam
Many environmental factors affect the morphology of migratory fish species, such as salinity, water flow rate, and temperature. However, studies on changes in fish morphology under environmental variations from salt water to brackish water are still limited in many fish species, especially in *Terapon jarbua*. This study aims to investigate the differences in the morphological parameters of *T. jarbua* between the coastal sea (seawater) and lagoon (brackish water); and between male and female fish based on a landmark morphological approach. Additionally, the genetic diversity of *T. jarbua* populations in Central Vietnam was elucidated using the mitochondrial DNA cytochrome oxidase subunit I (mtDNA COI) sequence as a molecular marker. The analytical results indicated no sexual dimorphism in the *T. jarbua* population, yet conformational differences exist between the two studied aquatic species. The analysis of 42 mtDNA COI sequences collected from Central Vietnam identified 13 haplotypes with medium genetic diversity and low genetic differentiation between the Tam Giang lagoon and Thua Thien Hue coastal (Fst = 0.028) and not significant (p = 0.126). Most haplotypes obtained are present in reference populations, indicating a high genetic exchange between populations. We proposed that the *T. jarbua* population in Central Vietnam has a stable connection with neighboring populations (China, Taiwan, Philippines, Indonesia, Malaysia, Bangladesh, India, and Pakistan)