University of Hawaiʻi at Mānoa

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    Effect of chronic heat stress in roughskin sculpin (<em>Trachidermus fasciatus</em>)

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    The roughskin sculpin (Trachidermus fasciatus) belongs to the family Cottidae and is a small carnivorous catadromous fish distributed along the coasts of China, Korea, and Japan. The effects of heat stress on roughskin sculpin have yet to be systematically performed. This study aimed to explore the effects of chronic heat stress on the roughskin sculpin. The survival rate, gill histopathological changes, and heat stress protein gene expression patterns of roughskin sculpin were analyzed after different temperature stresses of 19 ℃, 22 ℃, 25 ℃, 28 ℃, and 31 ℃ for 0, 3, 6, 9, and 12 days. The results revealed that roughskin sculpin could not survive at 33 ℃. Chronic heat stress can cause severe damage to gill tissue, reducing the gills' respiratory efficiency. Moreover, the expressions of hspb1, hspb7, and hspb11 changed significantly after heat stress. In the 31 ℃ treatment group, as stress time increased, hspb1, hspb7, and hspb11 expression initially increased and then decreased. This study’s results provide a reference for the study of heat stress physiology of roughskin sculpin as well as a new idea and method for evaluating and breeding heat stress traits of roughskin sculpin

    Development and Utilization of Transcriptome SSR Markers in <em>Procambarus clarkii</em>

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    To accelerate the research process of molecular marker-assisted breeding in Procambarus clarkii, this study conducted transcriptome sequencing of P. clarkii muscle tissue using the Illumina NovaSeq platform. Microsatellites (SSRs) were analyzed for distribution and sequence characteristics using MIcroSAtellite (MISA), and genetic diversity was studied in seven cultured populations. The results showed that the sequencing yielded between 9.9 to 12.7 Gb of clean data, and after assembly, produced between 52,244 to 83,367 contigs, identifying a total of 11,304 SSR loci. The SSRs were predominantly dinucleotide and trinucleotide repeats, accounting for 33% and 27% of the total loci, respectively. The average observed heterozygosity (Ho) across the seven different regional aquaculture populations ranged from 0.374 to 0.502, while the average expected heterozygosity (He) ranged from 0.502 to 0.604. The average polymorphic information content (PIC) for the populations was greater than 0.450, indicating substantial genetic variability. According to Nei's genetic distance, an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster tree divided the seven different regional aquaculture populations into two major clades: one comprising Yunnan (YN) and Qianjiang (QJ) and the other comprising the remaining five populations. The study found that 93.52% of the variation originated within populations, with only 6.48% of genetic variation attributable to differences between populations. These results lay a foundation for further assessment of the genetic diversity of P. clarkii, innovative utilization of germplasm resources, and in-depth studies into its molecular genetics and evolutionary mechanisms

    Puka Puka Parade 2025 - Volume 80, Number 05

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    Mergers of Voiced and Voiceless Consonants in the Siamese, Lanna and Lao Languages

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    The voicing of voiceless sonorants (VVS) and the devoicing of voiced obstruents (DVO) in Tai languages not only reduced the number of consonants but also increased tonal distinctions . While DVO in Siamese has been dated to the 1440s–1480s (Tangsiriwattanakul 2020), VVS in Siamese and both VVS and DVO in other Tai languages have received comparatively little attention. This research aims to date the mergers between voiced and voiceless consonants by examining correspondences between graphs and sounds of Siamese, Lanna, and Lao, and analyzing whether graphs attested in inscriptions represented the same or different phonemes. The findings reveal that confusion between graphs representing voiced and voiceless sonorants emerged around the 1370s, while voiced and voiceless obstruents emerged around the 1390s in Siamese. In Lanna, VVS and DVO were evident by the 1410s CE, supported by a 1411 inscription, whereas Lao shows confusions in voiced and voiceless graphs by 1444 CE. Considering that these observed time gaps may stem from limited earlier inscriptions in Lanna and Lao, this study proposes that VVS and DVO likely occurred at approximately the same time across the three languages due to their geographical proximity. These findings align with existing hypotheses (Li 1977; Gedney 1989b; Bickner 1992; Tangsiriwattanakul 2020), which argue that the mergers of voiced and voiceless consonants occurred after the foundation of the Sukhothai and Ayutthaya Tai polities in north-central Thailand

    Native Hawaiians in the Northern Mariana and Ogasawara Islands: An Historical Perspective

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    This study focuses on Native Hawaiians recorded in the Northern Mariana and Ogasawara Islands from an historical perspective. In 1811, a group of Native Hawaiians were forcibly brought against their wishes to the Northern Mariana Islands from Kauaʻi. They were then removed by the colonial Spanish administration and incorporated into the larger native CHamoru and Carolinian populations of Guam. In 1830, other Native Hawaiians with European and American settlers immigrated to the Ogasawara Islands from the Kingdom of Hawaiʻi. Their mixed heritage descendants eventually became Japanese citizens in 1876, remaining until WWII when they were evacuated to Japan. Under American rule from 1946 to 1968, they were returned to Ogasawara and remain Japanese citizens today. The tenure of Hawaiians on Agrihan island in the Northern Marianas was relatively short termed with little evidence of direct impact on Spanish Colonial material culture. In contrast, Hawaiian-style grass houses and outrigger canoes soon became recognized by American and later Japanese settlers to the Ogasawara islands as truly unique to that place and time. It is argued here that this cross-cultural and multi-ethnic phenomenon is not an isolated event in the history or prehistory of Micronesia or Remote Oceania. The Mariana archipelago was initially settled by Austronesian and Southeast Asian peoples well before Hawai‘i and probably before the Ogasawara Islands. However, many of these islands were subsequently repopulated by European, American, Carolinian, and Asian peoples during which time CHamorus and Hawaiians actively participated in what was the greatest diaspora over open water on the planet until the age of air travel

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