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Legislative files: Other legislation: "Laws that Treat Hawaii Differently": Military and defense: Department of the Army
Get PDFEffects of dietary cysteamine addition to a plant-based diet on growth performance, body composition, hepatosomatic histological structure and metabolism of taurine synthesis in turbot (<em>Scophthalmus maximus</em> L.)
Get PDFThis present study was conducted to illustrate the effect of dietary cysteamine on feeding, growth, biochemistry composition, hepatosomatic histological structure and metabolism of taurine synthesis in juvenile turbot (Scophthalmus maximus L.) (2.0 ± 0.01 g) fed high plant protein diets. A fishmeal-based diet (72.5% FM) was designed as the control diet (DietFM). Four isonitrogenous and isolipidic diets consist of 14.0% FM, 23.0% soybean meal and 30.0% wheat gluten meal, which were formulated to substitute FM with 0.0%, 0.05%, 0.1% and 0.15% cysteamine hydrochloride (labeled as DietPP, DietCS-0.05%, DietCS-0.1% and DietCS-0.15% respectively). Fish were stochastically allocated into fifteen 200L tanks and fed twice daily (06:30 and 18:30) to apparent satiation for 9 weeks. Feed intake (FI) in fish-fed DietCS-0.05% was conspicuously higher than that in fish-fed DietPP (p0.05). No conspicuously upward trend was found with the increase of dietary cysteamine in FBW, SGR, WGR, FER, PER, and SR when there was a downward trend in CF, VSI, and HSI (p>0.05). A remarkable increase was discovered from 0.0% to 0.05% cysteamine inclusion in ash content (p0.05). Only the body taurine content showed a notable increase between fish-fed DietCS-0.05% and fish-fed DPP among the indexes of body taurine, liver taurine, and serum taurine content (p0.05). The cellular morphology and cell veins in the liver of fish-fed diets with cysteamine inclusion were clearer than those of fish-fed DPP. The results showed that 0.05% cysteamine addition in a high plant protein diet is optimal
Rapid and convenient detection of <em>Weissella ceti</em> with loop-mediated isothermal amplification
Get PDFWeissella ceti, a Gram‐positive lactic acid bacterium, has emerged as a novel pathogen causing Weissellosis in rainbow trout (Oncorhynchus mykiss). Until now, it has not been easy to distinguish W. ceti from other pathogens by the symptoms of the infected rainbow trout, especially at the primary stage of infection. Thus, a convenient and rapid identification method is needed to determine this pathogen. The current study demonstrated loop-mediated isothermal amplification (LAMP) superior specificity and sensitivity for W. ceti. Primers were designed targeting the hemolysin A gene, and LAMP was accomplished within 30 min at 63 ℃. The minimum concentration of LAMP for W. ceti DNA was 5.9 x 10-6 ng/μl, which was 100 times more sensitive than traditional PCR. LAMP confirmed the high specificity for W. ceti, whereas the other six pathogens were negative reactions. Furthermore, the effectiveness of LAMP was also demonstrated in simulated clinical samples. Based on the results, LAMP was rapid and convenient for point-of-care testing to diagnose W. ceti infections
Legislative files: Other legislation: "Laws that Treat Hawaii Differently": Federal responses to survey: General: Department of Justice
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No full textLegislative files: Other legislation: "Laws that Treat Hawaii Differently": Appendix: Federal statutes - Hawaii (Congressional Research Service printouts)
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Effect of Water Exposed to Electric Current on Gamete Quality of Rainbow Trout (<em>Oncorhynchus mykiss</em>) and Water Parameters
Get PDFElectrofishing is a widely preferred method in freshwater. Freshwater conducts electric current at low levels. Therefore, a relatively high voltage electric current is applied in these waters. In this study, the effects of changes in the structure of the water after the electric current applied to the water on gamete quality and embryo-larvae survival rates were investigated. Water samples were collected from the central region of the tank (N), as well as from the positive (+) and negative (-) poles following the application of an electric current to the well water at 165 volts and 3 mA for durations of 15 and 30 minutes. Sperm kinematic velocity parameters, fertilization and hatching rates were determined to be at the highest level in the group where electric current was applied for 30 minutes and water samples were taken from the middle of the tank, 30 (N), were used (P0.05)
Application of Improved YOLOv8n-seg in Crayfish Trunk Segmentation
Get PDFThe crayfish industry (Procambarus clarkii) is experiencing rapid growth. However, the processing sector continues to face challenges due to a lack of advanced automation, relying heavily on manual visual inspection to assess crayfish specifications and integrity, which limits efficiency and precision in decision-making. To address the issue of intelligent grading of P. clarkii, this work proposes the GHB-YOLOv8-seg algorithm for segmenting the main trunk of P. clarkii shrimp based on the YOLOv8n-seg model. The original main trunk network is replaced through the coupling of Ghost and HGNetV2, and depth-separable convolution is employed to perform the linear transformation of the features. This results in a reduction in the number of parameters and computational complexity while maintaining high accuracy. The computational complexity is reduced; concurrently, introducing the weighted bidirectional feature pyramid network (BiFPN) enables the model to perform multi-scale feature fusion with greater alacrity, thereby enhancing the model's performance. Ultimately, the intelligent grading of crayfish specifications was achieved by calculating the pixel area after segmentation and converting it to the actual body weight. The results demonstrated that the number of parameters of the improved YOLOv8n-seg model was reduced by 60.5%, the model size was reduced by 55.4%, and the mAP value was increased from 98.9% to 99.2%. The study indicates that the YOLOv8n-seg algorithm model facilitates precise and lightweight segmentation of the crayfish trunk, which can be integrated into diverse mobile devices
