83602 research outputs found
Sort by
In Vitro Testing of Potential Probiotic Bacteria against Vagococcus salmoninarum in Rainbow Trout (Oncorhynchus mykiss, Walbaum 1792)
Get PDFIn this study, probiotic properties of endogenous microbiota of rainbow trout against Vagococcus salmoninarum isolated during an outbreak of vagococcosis in a trout farm in the Mediterranean region were evaluated. The candidate probiotic bacteria were isolated from rainbow trout intestines. A total of 157 isolates were obtained and screened for antagonistic activity against V. salmoninarum via the Well Diffusion Agar method. Six isolates were determined for antagonistic activity against V. salmoninarum. Conventional microbiological tests and API 20 Strep tests (bioMe´rieux) were used for further phenotypic characterization of all six antagonistic isolates. For molecular identifications of isolates, L. garvieae specific PCR and 16S rRNA gene sequence analysis were used. Antagonistic strains were identified including TUB/2013/V47 (L. garvieae), TUB/2013/V27 (L.garvieae), TUB/2013/V10 (L. garvieae), TUB/2013/V2 (L. garvieae) TUB/2013/V1 (L. lactis) and TUB/2013/V4 (L. lactis). The strains were then tested for hydrophobicity, bile salts and acid tolerance and antimicrobial activity. All isolates were congo red-positive, indicating the presence of hydrophobic structures in their cell walls. It was determined that whole antagonistic strains were resistant to low pH conditions and 0.6-1.5% bile concentrations. Antimicrobial test results showed that most of the strains are susceptible in vitro to amoxicillin/clavulanic acid ampicillin, doxycycline, erythromycin and florfenicol, which are frequently used in aquaculture. As a result, it was found that strains have in vitro probiotic properties (hydrophobic, tolerant to bile salts and low pH conditions). Further study is needed to explore their in vivo probiotic effects against vagococcosi
Effect of Extender Supplemented with Different Sugar Types on Post-thaw Motility, Viability and Fertilizing Ability of Cryopreserved Common Carp (Cyprinus carpio) Spermatozoa
Get PDFThe influence of various sugar types supplemented to the extender on post-thaw motility, viability, and fertilizing capacity of cryopreserved common carp (Cyprinus carpio) semen were investigated. The results indicated that types of sugar significantly influenced motility, motility duration, and viability rates (P<0.05). Glucose, maltose, sucrose, and trehalose provided higher motility compared to the sugar-free control in post-thaw samples. Trehalose provided highest progressive motility duration, and higher viable sperm rates were obtained with all sugar types except xylose. Xylose exhibited the lowest post-thaw progressive motility duration (35.2±1.4s). The mean highest fertilization (78.2±1.4%) and eyed egg rates (94.3±1.5%) were determined using trehalose in the extender, and differences between the treatments in the fertilization and eyeing rates were significant (P<0.05). Finally, the present study showed that sugars, especially maltose and trehalose (disaccharide), improved post-thaw spermatozoa motility and fertility in common carp semen
First Report of Chryseobacterium sp. from Koi (Cyprinus carpio) in Turkey
Get PDFIn this study, we isolated Chryseobacterium sp. from koi in Turkey. The disease outbreak occurred in fish weighing 10-300g (water temperature 9-10°C) in November 2011- February 2012. The cumulative mortality rate was approximately 55% over 4 months. Infected koi exhibited anorexia, weakness, emaciation, damage to dorsal and caudal fins, grey-white skin discoloration (1-1.5cm) in the head area, large open wounds in skin with disease progression, necropsy findings, paleness of the liver, enlarged spleen and kidney, acidic fluid in the body cavity. Samples for bacteriological examinations were collected from the kidney, liver, and spleen using sterile swabs; these samples were streaked onto Anacker ordal Agar and incubated at 18°C for 48 h. Five bacterial isolates were obtained from diseased fish. Phenotypic characteristics of the isolates were determined by conventional methods and rapid identification kits, API 20NE, and API ZYM. Comparative 16S rRNA gene sequence analysis demonstrated that isolate Sin57 belonged to the genus Chryseobacterium, with highest sequence similarity (98.5 %) to C. aahli T68T and C. limigenitum SUR2
2016-2017 Entering Class and Transfers
No full textThis collection comprises the photo guides of the William S. Richardson School of Law Entering Classes from 1973-2018. Each photoguide is accessible as a pdf and is text searchable. This collection will be updated as a new photo guide is created and uploaded each year
