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Effects of Trehalose, Bovine Serum Albumin, and Sucrose, on the Integrity of the Plasma Membrane of Pseudosciaena crocea Semen after Cryopreservation
Get PDFCryopreservation and the effect of additives on the plasma membrane stability, motility, enzyme activity, membrane lipid composition, and ultrastructural injury of sperm of Pseudosciaena crocea, were examined in this study. Results indicated that the addition of trehalose (TH) and bovine serum albumin (BSA) provided significantly better results in the motility test compared to sucrose. Some BSA groups, showed significantly increased semen motility and effective enzyme activity. There were no significant differences between the cholesterol (CHO) composition for fresh sperm and cryopreserved sperm stored in 3 g/L BSA or 8.56 g/L TH+3 g/L BSA. Ultrastructural injury included damaged membrane, lost mitochondria, or broken flagella, whereas the majority of the freeze-thawed sperm remained morphologically normal. In conclusion, the addition of 3 g/L BSA and 8.56 g/L TH+3 g/L BSA significantly increased semen quality
Lysine Supplementation of the Protein Concentrate of Crinkle Grass Rhizoclonium riparium var implexum as an Ingredient in the Diet of Nile Tilapia Fry
Get PDFA study was conducted on 1000 fish, to evaluate the effect of supplementing crystalline lysine (Lys) to a diet containing protein concentrate from seaweed Rhizoclonium riparium var implexum (RPC) on the performance of Nile tilapia fry. A previous finding showed that protein concentrate was deficient in lysine. Four experimental diets were prepared: Diet 1 - no RPC and no Lys; Diet 2 - 8.4% RPC+0.5% Lys; Diet 3 - 8.4% RPC+1.0% Lys; and Diet 4 - 12.6% RPC+1.5% Lys. After a 60 day feeding trial, results showed that final average body weight, weight gain, protein accumulation, and whole body biochemical composition were not affected by the dietary treatment. Specific growth rate, food conversion ratio, and protein efficiency ratio were all significantly higher in fish fed the test diets (i.e. Diets 2, 3 and 4) than in those fed the control diet. There were no significant differences in protein and energy retention; lipid retention was lowest in Diet 2 group and statistically similar to Diet 3 and 4 groups. In conclusion, most parameters improved significantly in fish fed diets with RPC and Lys supplementation compared to fish in the control group. The most cost effective dietary treatment was Diet 2 containing 8.4% Rhizoclonium protein concentrate supplemented with 0.5% crystalline lysine
Aeromonas hydrophila: a Causative Agent for Tail Rot Disease in Freshwater Cultured Murray Cod Maccullochella peelii
Get PDFTail rot disease is the cause of significant economic damage in freshwater farmed Murray cod Maccullochella peelii. Only scarce information is available on Aeromonas hydrophila as a possible causal agent for this disease. In this study, a virulent strain, temporarily named XY3, was isolated from diseased codfish suffering from tail rot disease, and identified as A. hydrophila through phylogenetic analysis and phenotypic characteristics. A. hydrophila possesses multiple virulence genes including aerA, ahpA, alt, ast and hlyA genes. In addition, it appears that isolate XY3 has developed multiple resistances to cephalosporin, chloromycetin, glycopeptides, macrolides, nitrofuran, and penicillin drugs, as well as to aminoglycosides, sulfonamides, and tetracyclines antibiotics for veterinary uses in aquaculture as revealed when screened against a range of common antibiotics. To the best of our knowledge, this is the first report of tail rot disease caused by A. hydrophila in freshwater farmed codfish
Institute for Research, Extension, and Training in Agriculture's South Pacific agricultural news
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Book review: From King Cane to the Last Sugar Mill: Agricultural Technology and the Making of Hawaiʻi's Premier Crop
Get PDFA Decolonial Pin@y's Journey
Get PDFChris Lipat, a member of Decolonial Pin@y, shares her account on the work and accomplishments of this Filipina/x led organization based in Honolulu, Hawai'i
Terevaka Archaeological Outreach (TAO) 2016 field report: Exploring a new dimension
Get PDFSince 2003, Terevaka Archaeological Outreach (TAO)
has provided educational opportunities for local high
school students within the Rapa Nui community.
The program was created in an effort to restructure
the traditional priorities of international scientific
expeditions to the island, and since 2003 the three
primary goals of TAO have remained the same: (1)
To offer experiential learning opportunities specific to
cultural and natural resources that surround the local
community; (2) To promote awareness and expertise in
conservation measures and sustainable development;
and (3) To document and study both cultural and
natural phenomena of the past and today