University of Hawaiʻi at Mānoa

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    Letter to Colonel Farrant L. Turner

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    Letter to Colonel Farrant L. Turner from Yost regarding Sgt. Heiji Fukuda's stepfather and stepbrother who were being held in a concentration camp by the US government. Yost was requesting the colonel's help in getting them released. Col. Turner's reply informed Yost that there was a chance of release

    Letter to Yost

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    Letter from George Aki to Yost. He mentions the "relocation centers" or the US version of concentration camps where many Japanese-Americans were interned, including relatives of the soldiers in the 100th battalion

    Letter To Satsuyo Okamoto

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    Condolence letter to Mrs. Satsuyo Okamoto regarding the dath of her son Donald M. Okamoto

    Typed Transcript of Letter to Matsue Shimabukuro

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    Condolence letter to Mrs. Matsue Shimabukuro regarding the death of Hideo Shimabukuro

    Typed Transcript of Letter to Ruth Terada

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    Condolence letter to Mrs. Ruth Terada regarding the death of her son Henry Terada

    Typed Transcript of Letter to Matsu Makishi

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    Condolence letter to Mrs. Matsu Makishi regarding the death of Matsutada Makishi, and $10.00 that a friend owed him and requested to be sent to his next of kin

    A Review: Factors Affecting Outbreaks of Saprolegniosis on Aquatic Animals

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    In this paper, we review the current knowledge regarding factors affecting the outbreaks of saprolegniosis. Saprolegniosis is a serious disease which can cause significant economic losses in aquaculture. Oomycetes are considered to be responsible for the disease. Outbreaks of saprolegniosis occur when equilibrium between pathogenic oomycetes and host resistance is disturbed. This may be the result of an increase in numbers or virulence of the zoospores of oomycetes in the water, or an increase in the susceptibility of the host. The presence of oomycetes zoospores in the environment is regarded as one of the most important causes of saprolegniosis. However, important differences have been found between plant and animal pathogenic oomycetes and appear to be related not only to tactic responses to environment, but also to infection strategies to a host. In addition, fish are more susceptible to infection by pathogenic oomycetes when physically injured, stressed or infected. Furthermore, water environmental factors, such as temperature, salinity, and microorganisms, should be carefully considered since they play a key role in the outbreaks of saprolegniosis

    Recovery of Listonella anguillarum from Diseased Pufferfish (Takifugu rubripes) in China

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    In May 2016, there was a disease outbreak among pufferfish (Takifugu rubripes) in a marine farm in Tianjin municipality, with the cumulative mortality rate reaching 45% within 7 days from the beginning of the outbreak. The main symptoms of the disease were abdominal and anal swelling of the diseased fish. Significant damage was also observed histopathologically in the intestine, liver, and kidney. The strain H008 was isolated from diseased kidneys, and the challenge test revealed that the same disease was diagnosed in pufferfish which suffered similarly high mortality rates. Using physiological biochemical tests combined with 16S rDNA sequence analysis, the strain was identified as Listonella anguillarum. Antimicrobial susceptibility tests showed that H008 was resistant to 10 of 15 antimicrobial agents tested. To the best of our knowledge, this is the first report of L. anguillarum causing disease in pufferfish

    Providencia alcalifaciens: a Causal Agent of Red Leg Disease in Freshwater-Cultured Whiteleg Shrimp Penaeus vannamei

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    Red leg disease causes significant economic losses in whiteleg shrimp Penaeus vannamei. Yet only scarce information is available on Providencia alcalifaciens as a bacterial pathogen of this disease. In this study, a virulent strain, temporarily named P3, was isolated from diseased freshwater-cultured P. vannamei suffering from red leg disease, identified phenotypically and molecularly as P. alcalifaciens. A phylogenetic tree was constructed to examine the taxonomic position and relatedness of isolate P3 to other P. alcalifaciens isolates. When screened against a range of common veterinary antibiotics, isolate P3 exhibited susceptibility to aminoglycosides and quinolones antibiotics in aquaculture. To the best of our knowledge, this is the first report of P. alcalifaciens as a causal agent of red leg disease in freshwater-cultured P. vannamei

    Efficacy of Tricaine Methanesulfonate (MS-222) as an Anesthetic Agent for Short-term Anesthesia in Juvenile Yellow Perch (Perca flavescens)

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    The present study was conducted to assess an optimal dose of tricaine methansulfonate (MS-222) for rapid anesthesia and recovery in juvenile Yellow perch Perca flavescens. Yellow perch (≈7.0 g/fish) exposed to increasing concentrations of MS-222 (100, 150, 200, 250 and 300 mg/L) showed significant (P<0.05) decreased anesthetic induction and increased recovery time with increasing MS-222 dose. Among the doses tested, the 250 mg/L MS-222 dose showed the shortest anesthetic induction and recovery times. Blood glucose and hematocrit levels were significantly (P<0.05) lower in juvenile yellow perch exposed to the 250 mg/L MS-222 dose than the other MS-222 doses tested. Juvenile yellow perch (about 9.7 g/fish) exposed to 250/mg MS-222 combined with bicarbonate buffer at three ratios (w/w: 1:0.5, 1:1 and 1:2) showed significantly (P<0.05) reduced anesthesia induction and recovery times. The shortest times of anesthesia induction and recover were observed in treatments using the 1:1 MS-222 to buffer ratio. Analysis on serum biochemical parameters (e.g., osmolality, glucose, albumin, calcium, alanine transaminase, total protein and calcium) showed that use of sodium bicarbonate with MS-222 did not have any significant effect on the above measurements compared with those determined in fish exposed to MS-222 only. Anesthesia induction time and recovery time of yellow perch were significantly affected by body sizes. These results suggest that a brief exposure to a 250 mg/L MS-222: sodium bicarbonate dose may be optimal for minimizing handling disturbance in juvenile yellow perch

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