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Isolation and Characterization of Polymorphic Microsatellites from Draft Genome Data for Large Yellow Croaker, Pseudosciaena Crocea
Get PDFIn this study, we isolated thirty-eight microsatellite loci from the draft genome of large yellow croaker, Pseudosciaena crocea. These loci were tested on 34 individuals from Zhangzhou prefecture city in Fujian. Most loci were highly polymorphic. A total of 214 alleles were detected with an average of 5.63 alleles per locus. The observed and expected heterozygosity ranged from 0.454 to 0.909 and from 0.503 to 0.918, with an average of 0.635 and 0.744, respectively. The PIC values of these loci ranged from 0.372 to 0.878, with an average of 0.712. Eight loci significantly deviated from Hardy-Weinberg equilibrium (HWE). Significant linkage disequilibrium (LD) was not found in these 38 loci. The newly identified polymorphic markers will contribute to the study of genetic diversity, population structure, and conservation of P. crocea
Pathogenicity and Immune Response of Starry Flounder, Platichthys stellatus, Infected with Vibrio anguillarum
Get PDFVibrio anguillarum is the aetiological agent of vibriosis, a disease affecting many marine fish species. The occurrence of vibriosis in starry flounder, Platichthys stellatus, grown in an aquaculture farm has demonstrated the urgent need for information on pathogenic infection and immune response for efficient disease management. This is the first study to report Vibrio anguillarum isolation and infection in starry flounder. We evaluated immune responses, serum biochemical parameters, and cumulative mortality of the fish by experimentally challenging healthy fish. The expression levels of five immune genes (TNF, TNFR, IL-6, MHCII, and CXC) were measured by real-time quantitative PCR. The transcriptional levels of the genes encoding tumor necrosis factor (TNF), TNF receptor (TNFR), interleukin-6 (IL-6), the major histocompatibility complex (MHC-II), and a chemokine (CXC) in the head-kidney of V. anguillarum infected fish were significantly upregulated compared with control fish and biochemical indices including the alanine aminotransferase, total serum protein, and glucose levels of infected fish differed significantly from those of control. Additionally, Starry flounder infected with V. anguillarum at 1.67 × 106 and 1.67 × 108CFU/mL showed 53%, and 100% mortality, respectively. This study furthers our understanding of the immune and serum biochemical alterations, and mortality induced by bacterial infections, depending on pathogen concentration. This may advance strategies for control of V. anguillarum in cultured starry flounde
Effects of Dietary Pantothenic Acid Supplement on Hepatic Antioxidative Abilities and Intestinal Microflora in Juvenile Golden Pompano (Trachinotus Ovatus)
Get PDFThis study explored the effects of dietary pantothenic acid (PA) supplement on juvenile golden pompano (Trachinotus ovatus). Six isonitrogenous and isolipidic diets containing six graded levels of pantothenic acid (0, 16.4, 20.0, 26.0, 33.4 and 37.0 mg/kg) were formulated. Juvenile golden pompano (8.80±0.10g) were randomly assigned into six groups with three replicates in each group and 20 fish in each replicate. The fish were fed twice a day for 8 weeks. The results indicated that PA deficiency caused clubbed gills with interlamellar proliferative lesions. Survival rate increased significantly (P＜0.05) when PA levels were increased. Dietary PA significantly increased the activities of hepatic acid phosphatase (ACP), alkaline phosphatase (ALP), peroxidase (POD) and glutathione reductase (GR) (P＜0.05) and their activities reached the maximum in the Diet-P4 group. A diet supplemented with 20.0 and 26.0 mg/kg PA significantly decreased (P＜0.05) the activity of hepatic MDA. Dietary PA also increased the diversity and abundance of intestinal microflora and inhibited the growth of harmful bacteria. In conclusion, PA deficiency caused lesions of gills and optimal PA supplement in diet increased hepatic antioxidative abilities and improved intestinal microflora of juvenile golden pompano
