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Development and characterization of 20 microsatellite markers in spotted sea bass (<em>Lateolabrax maculatus</em>) and cross-amplification in related species
Get PDFThe spotted sea bass (Lateolabrax maculatus) is an economically valuable cultured fish species in China. In this study, 20 novel polymorphic microsatellite loci of L. maculatus were isolated from genomic data and characterized using 40 wild individuals. The number of alleles and the effective number of alleles ranged from 2 to 12 (average of 5.1000) and from 1.180 to 8.000 (average of 3.3097). The observed and expected heterozygosities ranged from 0.083 to 0.875 (average of 0.4405) and from 0.153 to 0.875 (average of 0.5633), respectively. Deviation from the Hardy-Weinberg equilibrium was observed in 11 loci (P Lates calcarifer, achieved successful amplification of 16 primers. The microsatellite markers developed in this study could be used for research on genetic breeding of L. maculatus and genetic relationships among tested taxa
Analysis of Some Immune Parameters in The Skin Mucus of Four Cultured Fish Species
Get PDFThe skin mucus layer and its components provide the first line of defense against fish pathogens. The skin mucus samples were taken from four species Nile tilapia (Oreochromis niloticus), rainbow trout (Oncorhynchus mykiss), Siberian sturgeon (Acipender baerii), and the European sea bass (Dicentrarchus labrax) for analyzing of some immune parameters. It is measured the activities of alkaline phosphatase, lysozyme, myeloperoxidase, bactericidal activity (against Aeromonas hydrophila), total protein and immunoglobulin concentration. The sea bass had highest level of lysozyme, myeloperoxidase, and bactericidal activities, and so were the total protein and immunoglobulin concentrations. The alkaline phosphatase activity in skin mucus measured in Nile tilapia as maximum level while the immune activity observed in sea bass among fish species. The skin mucus determined a more robust bactericidal activity against A. hydrophila fish pathogen in all fish species. The current study results presented preliminary information for better comprehension of the function of skin mucus as a key component in the different fish immune systems in aquaculture
Index to the Lahaina News (1979-2003), the Lahaina Sun (1970-1973), and the Lahaina Times (1980-1983, limited issues)
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Status and Gender in Hawaiian House Complexes
Get PDFThis research compares excavated material collected from Sites 50-50-10-2090 and -2091 (Kēōkea Maui), a pre-European contact (c. 1650 C.E.) kauhale house complex, to a model of expected findings derived from Hawaiian oral traditions and ethnohistoric accounts of household gender and status activities. Our goal is to critically analyze the relationship between the Hawaiian system of ‘ai kapu (taboo) and the use of space within house sites to better understand the diversity of the daily lived experiences and the multiplicity of gender and status interactions that household members engaged in. Results indicate a distinct post-1650 C.E. intra-site distribution of faunal remains that is concomitant with status and potentially gender food consumption. This patterning, however, does not ideally match the model derived from ethnographic descriptions
