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Preface: Writing ASAO Histories
Get PDFKeeping the written history of the Association for Social Anthropology in Oceania has been on the agenda for ASAO since the very earliest days of the organization’s existence. This essay recounts various efforts to document that history and to compile significant records, such as a bibliography of publications arising from ASAO sessions. It then describes the process behind the development of the papers in this collection over the course of several ASAO sessions and briefly describes the focus of each essay. Paper topics range from the nature of ASAO sessions, annual meeting site selection, and the distinguished lecture series; to issues of inclusiveness and rights of membership, Pacific Islander participation, and student mentoring; to ASAO book series and other forms of communication including newsletters, the ASAONET listserve, and the ASAO website
Characterization and expression analysis of heat shock protein 90β from golden pompano (<em>Trachinotus ovatus</em>, Linnaeus 1758) in responses to stock density stress and <em>Cryptocaryon irritans</em> infection
Get PDFHeat shock protein 90 family, a class highly conserved protein and important molecular chaperone and its plays a role in enhancing animal adaptability to environmental stress and improving immunity. In this study, a full-length HSP90β cDNA (ToHSP90β) sequence was identified from Trachinotus ovatus. The cDNA of ToHSP90β was 2648 base-pairs (bp) long and contained a 5′-terminal untranslated region of 130 bp, a 3′-terminal untranslated region of 334 bp, and the open reading frame length 2184 bp, which can encode 728 amino acids. The ToHSP90β has a heat shock protein 90 family signature. The ToHSP90β amino acid sequence has high homology with other fish HSP90β amino acids (92.5-99.17%) and contains five conserved signal regions and a structural feature of the cytoplasmic HSP90 family. Phylogenetic tree analysis indicated that ToHSP90β was most closely related to Seriola dumerili HSP90β. Tissues expression analysis showed that the ToHSP90β was expressed in all nine tissues of T. ovatus, and its expression was the highest in the intestine. The stocking density significantly affected the expression of ToHSP90β in the kidney and brain tissues, and its expression levels increased significantly with increasing stocking densities, indicating that it plays an important regulatory role in the process of T. ovatus’ chronic stress response. After Cryptocaryon irritans infection, the mRNA expression of ToHSP90β was significantly increased in liver and kidney tissues, indicating that ToHSP90β would be involved in the immune regulatory response. This study was the first to analyze the expression pattern of HSP90β mRNA in T. ovatus tissues and their expression responses to stocking density stress and C. irritans infection. To provide a theoretical basis for further study of the function of fish HSP90 and its physiological mechanism in environmental stress and parasite infection
Effects of substituting dietary fish meal with soybean meal isolate on growth performance, digestive enzyme activity, and intestinal morphology of spotted seabass (<em>Lateolabrax maculatus</em>)
Get PDFSoy protein isolate (SPI) is a promising plant protein source to replace fish meal (FM) in aquatic feeds. This study investigated the growth performance, digestive enzyme activity, and intestinal morphology of spotted seabass (Lateolabrax maculatus) fed diets with FM partially substituted by SPI. Three iso-nitrogenous and iso-lipidic diets were formulated to replace 0, 25, and 50% of FM with SPI, being abbreviated as FM, SPI25, and SPI50, respectively. Each diet was allocated to triplicates of fish for 8 weeks. The results indicated that the weight gain was gradually reduced with increasing dietary SPI levels, as did intestinal trypsin and lipase activities. However, these traits were not significantly decreased until 50% of FM was substituted by SPI. Furthermore, feed intake and lipid digestibility significantly decreased with dietary SPI inclusion. Moreover, replacing 50% of dietary FM with SPI significantly reduced the intestinal villus height and perimeter ratio, whereas these traits did not markedly differ between FM and SPI25 groups. In conclusion, this study indicated that up to 25% of dietary FM could be substituted with SPI without significantly affecting the growth, digestive enzyme activity, or intestinal morphology in spotted seabass
Effect of Power Pump and Nozzle Diameter Microbubble Generator to Increase Oxygen Concentration in Aquaculture
Get PDFVarious types of microbubble generators for use in aquaculture facilities for aeration. The aeration system is essential for high-density shrimp cultivation. However, there is a blockage in the microbubble generator aeration system, and generally, aeration at high densities requires considerable electrical power. In this study, modifications were made to various diameter nozzles and various pump power for microbubble generators. This research was conducted to determine the best microbubble by comparing the nozzle diameter and pump power. To achieve this goal, three diameter nozzles are combined with three different powers with an airflow rate of 2.5 LPM. The best combination of 32.5 mm nozzle diameter and 160-watt pump power. Produce bubbles ranging from 5.6 – 82 μm, with an increased oxygen concentration time of 60 minutes
Congressmen Kahele and Case announce millions in federal funding to support efforts to combat COVID-19
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