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Molecular and functional characterization of allantoate amidohydrolase from Phaseolus vulgaris
No full textEmbargado hasta 01/01/2100Allantoate degradation is an essential step for recycling purine-ring nitrogen
in all plants, but especially in tropical legumes where the ureides allantoate
and allantoin are the main compounds used to store and transport the
nitrogen fixed in nodules. Two enzymes, allantoate amidohydrolase (AAH)
and allantoate amidinohydrolase (allantoicase), could catalyze allantoate
breakdown, although only AAH-coding sequences have been found in plant
genomes, whereas allantoicase-related sequences are restricted to animals
and some microorganisms. A cDNA for AAH was cloned from Phaseolus
vulgaris leaves. PvAAH is a single-copy gene encoding a polypeptide of 483
amino acids that conserves all putative AAH active-site domains. Expression
and purification of the cDNA in Nicotiana benthamiana showed that the
cloned sequence is a true AAH protein that yields ureidoglycine and ammonia,
with a Km of 0.46 mM for allantoate. Optimized in vitro assay, quantitative
RT-PCR and antibodies raised to the PvAAH protein were used to study AAH
under physiological conditions. PvAAH is ubiquitously expressed in common
bean tissues, although the highest transcript levels were found in leaves. In
accordance with the mRNA expression levels, the highest PvAAH activity and
allantoate concentration also occurred in the leaves. Comparison of transcript
levels, protein amounts and enzymatic activity in plants grown with different
nitrogen sources and upon drought stress conditions showed that PvAAH
is regulated at posttranscriptional level. Moreover, RNAi silencing of AAH
expression increases allantoate levels in the transgenic hairy roots, indicating
that AAH should be the main enzyme involved in allantoate degradation in
common bean
Canonical correlation of technological innovation and performance in sheep's dairy farms: Selection of a set of indicators
No full textEmbargado hasta 01/01/2100Integrated dairy sheep system in Castilla La Mancha (Spain) suffers from a crisis based on lack of profitability. Technological innovation is a key factor to increase farm's viability. It requires the identification of the appropriate technologies to develop, later on, a set of technological indicators associated to results that support the decision-making process. The objectives of this research were: First, the selection of innovations and its grouping in a technological innovation set. Second, assess the relationship with other indicators; mainly technological and structural, productive and economic performance data set.
Information from 157 dairy sheep farms in Castilla La Mancha was used. The questionnaires included 77 pre–selected technological innovations and 150 questions on productive, economic and social data. The selection of innovations and its grouping into technological innovation areas, took place according to a qualitative, consensus and participatory methodology. Canonical correlation is a multivariate statistical technique used for analyzing the relationship amongst different set of indicators. 38 technologies were identified, grouped into a set of six indicators (T1, management; T2, feeding; T3, animal health; T4, land use; T5, milking equipment; T6, reproduction-genetic). Results derived from the canonical correlation showed a robust relationship between technological and structural, productive and economic performance data set. Land use, reproductive-genetic and management were amongst the main technologies that mainly explained this relationship, followed by those associated to milking equipment and dairy. Technological innovation demands a proper structure and an integral vision of dairy system that considers interactions amongst technological areas. It would be necessary to go deep in its knowledge in order to increase farm's viability
Peripheral oxidative stress in relapsing–remitting multiple sclerosis
No full textEmbargado hasta 01/01/2100.Objectives: To evaluate levels of oxidative stress in blood samples in patients with relapsing–remitting MS (RR-MS). Design and methods: Peripheral blood samples were collected from 24 RR-MS patients and 15 healthy controls. Levels of the following were measured: carbonylated proteins, 8-hydroxy-2′deoxyguanosine (8OHdG), total glutathione, reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG ratio, superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRd), glutathione-S- transferase (GST), myeloperoxidase (MPO), antioxidant gap, total antioxidant capacity (PAO), global oxida- tive stress (GOS), serum vascular cell adhesion molecule-1 (sVCAM-1) and serum inter-cellular adhesion molecule 1 (sICAM-1).
Results: Values for carbonylated proteins, 8OHdG, total glutathione, GSH, GSH/GSSG ratio, SOD, GRd and GOS were significantly higher in RR-MS patients than in healthy controls. By contrast, PAO, GSSG, GPx and GST were lower in RR-MS patients.
Conclusion: Oxidative stress plays a major role in MS and is observed prior to relaps
A Weierstrass type representacion for translating solitons and singular minimal surfaces
No full textEmbargado hasta 01/01/2100En este artículo proporcionamos una fórmula de representación de Weierstrass para solitones traslación y superficies mínimas singulares en el espacio Euclídeo R3. Como aplicación estudiamos cuando la aplicación de Gauss tiene un argumento armónico y resolvemos un problema general de Cauchy (problema de Björling) en esta clase de superficies
EHP-101, an oral formulation of the cannabidiol aminoquinone VCE-004.8, alleviates bleomycin-induced skin and lung fibrosis
No full textEmbargado hasta 01-01-2100Systemic sclerosis (SSc) or scleroderma is a chronic multi-organ autoimmune disease characterized by vascular,immunological, and fibrotic abnormalities. The etiology of SSc is unknown, but there is growing evidence that dysfunction of the endocannabinoid system (ECS) plays a critical role in its development. Since the semi-synthetic cannabinoquinoid VCE-004.8 could alleviate bleomycin (BLM)-induced skin fibrosis, we have investigated an oral lipid formulation (EHP-101) of this dual PPARγ/CB2 receptors activator for the prevention of skin- and lung fibrosis and of collagen accumulation in BLM challenged mice. Immunohistochemistry analysis of the skin showed that EHP-101 could prevent macrophage infiltration as well as the expression of Tenascin C (TNC), vascular cell adhesion molecule 1 (VCAM1), and the α-smooth muscle actin (SMA). EHP-101 could also prevent the reduced expression of vascular CD31 typical of skin fibrosis. RNAseq analysis of skin biopsies showed a clear effect of EHP-101 in the inflammatory and epithelial-mesenchymal transition transcriptomic signatures. TGF-β- regulated genes [matrix metalloproteinase-3 (Mmp3), cytochrome b-245 heavy chain (Cybb), lymphocyte antigen 6E (Ly6e), vascular cell adhesion molecule-1 (Vcam1) and Integrin alpha-5 (Itga5)] were induced in BLM mice and repressed by EHP-101 treatment. By intersecting differentially expressed genes in EHP-101-treated mice with a dataset of human scleroderma intrinsic genes, 53 overlapped genes were discovered, including biomarkers of SSc like the C-C motif chemokine 2 (Ccl2) and the interleukin 13 receptor subunit alpha 1 (IL-13Ra1) genes. Taken together, these data provide a rationale for further developing VCE-004.8 as an orally active agent to alleviate scleroderma and, possibly, other fibrotic diseases as well
Ciudad líquida y liquidada: lugar histórico frente a superficie virtual
No full textEmbargado hasta 01/01/210
Estudio comparado del concepto de obligación civil en Derecho romano y Derecho vigente
No full textEmbargado hasta 01/01/2100Es objeto de esta aportación resumir el concepto de obligación, su evo-lución histórica y su régimen jurídico con fin instructivo y didáctico. Por otro lado, se pretende con cada uno de sus epígrafes evidenciar la importancia del Derecho Romano en los estudios de derecho por su influencia directa en nuestro ordenamiento jurídico vigente. A partir del siglo VI el Derecho Romano continúa vivo como referente directo del Derecho común de los pueblos germanas que dominaron Europa hasta que se institucionaliza como Ius Commune europeo a partir del siglo XII y se desarrolla a partir sobre todo del siglo XVI como base del Derecho de los estados europeos. En nuestro actual ordenamiento jurídico la vigencia indirecta del Derecho Romano se manifiesta en multiplicidad de principios y axiomas jurídicos recogidos legislativamente y mencionados en los considerandos de las sentencias de nuestro más alto tribunal. En materia de obligaciones, constatamos esta realidad y nos permitimos mostrar su regulación comparándola con los artículos correspondientes del Código Civil.The purpose of this chapter is to summarize the concept of obligation, its historical evolution and its legal regime for instructive and educational purposes. On the other hand, it is intended with each of its headings to show the importance of Roman Law in law studies by its direct influence on our current legal system. From the sixth century on, Roman Law continued to be a direct reference point for the common law of the Germanic peoples who dominated Europe until it was institutionalized as the European Tus Commune from the twelfth century onwards and developed from the six-teenth century onwards as a basis for Law of European states. In our current legal order, the indirect validity of Roman law manifests itself in a multi-plicity of legal principies and axioms, which have been incorporated into legislation and mentioned in the recitals of the judgments of our highest court. In terms of obligations, we confirm this reality and allow ourselves to show its regulation by comparing it with the corresponding articles of the Civil Code
La enseñanza bilingüe y la integración de las inteligencias múltiples: una aportación diferente en el ámbito educativo
No full textEmbargado hasta: 01/01/2100La implantación de la enseñanza bilingüe y el desarrollo de la Teoría de las Inteligencias Múltiples formulada por Howard Gardner, psicólogo e investigador de la Universidad de Harvard, han tenido un tremendo impacto en el ámbito educativo. La educación bilingüe se ha convertido en uno de los objetivos prioritarios en Europa, potenciando la creación de acciones para la mejora del aprendizaje y de la enseñanza de la lengua extranjera. Las bases psicopedagógicas basadas en los últimos avances de la neurociencia como la teoría de las Inteligencias Múltiples nos llevarán a comprender mejor el proceso de enseñanza y aprendizaje en los contextos de bilingüismo educativo. La teoría de que todos los seres humanos poseemos diferentes inteligencias que no son inamovibles sino educables revoluciona el ámbito educativo captando la atención de los educadores a nivel mundial. En definitiva, nos encontramos ante una nueva visión pluralista de la mente, que reconoce en los seres humanos diferentes potenciales cognitivos y que ha repercutido considerablemente en contextos de bilingüismo educativo. En esta obra se establece una aproximación a la definición de los conceptos de bilingüismo, plurilingüismo y multilingüismo analizando los modelos y tipos que podemos diferenciar, así como los factores y las variables que influyen en este campo y se analiza la repercusión que la teoría de las Inteligencias múltiples ha tenido en la enseñanza bilingüe, así como su aplicación en el aula
