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Léxico de El rapto de Helena de Coluto
Full text linkEl léxico de El rapto de Helena de Coluto recoge las voces de la obra de acuerdo a la
edición francesa de Orsini (1972), nuestra edición de referencia. En la organización
interna de un artículo se contemplan los siguientes apartados: 1. métrica y frecuencia, 2.
morfología, 3. cuerpo del artículo que incluye: significados, indicaciones sintácticas,
semánticas o contextuales, contextos griegos, referencias al texto e información textual
significativa y opcionalmente según los datos obtenidos del trabajo de investigación
realizado: 4. loci similes y 5. comentario. Para ello se ha consultado bibliografía
especializada que ilustra el objeto de investigación y se han seguido los principios
lexicográficos y las convenciones formales del Diccionario Griego-Español
Design and formulation of nanocarriers for the treatment of diabetic eye diseases
No full textThe eye is a crucial organ that allows us to experience the world visually,
and the loss of vision is a fear for many. One significant threat to eye health
is diabetic retinopathy, which can result in consequences as severe as
blindness if left untreated. Current treatments for diabetic retinopathy
involve injections and laser exposure, but they only slow down the
degeneration of the retina. Therefore, there exists a need for preventive
treatments to protect patients' vision. One potential solution is the use of a
small molecule called epalrestat, which can block a metabolic pathway
involved in the development of diabetic retinopathy. To enhance the delivery
of epalrestat to the eye, drug carriers have been investigated. Niosomes and
oleogels were formulated, characterized, and tested in various models
including ex vivo, in ovo, and in vivo assays. The self-assembly process of
the niosomes was investigated with the help of a monolayer model. These
carriers provided a saf e and e:fficient way to help with the transport of
epalrestat across the various ocular barriers.2025-01-1
Dispersal limitation shapes distance‐decay patterns of European spiders at the continental scale
Full text linkAim
To assess the relative relevance of dispersal limitation and species sorting as drivers of spatial turnover between spider faunas of European territories.
Location
Continental Europe.
Time period
Present.
Major taxa studied
Spiders (Order Araneae).
Methods
We analysed how distance-decay patterns differ between northern and southern Europe (broadly, territories covered vs. not covered by ice sheets during the last glacial maximum, respectively) in 15 spider families, using standardized distances to allow a direct comparison between parameters (i.e. slope and intercept) of climatic and spatial distance-decay models. Thus, we assessed North–South differences in parameters of spatial and, independently, climatic distance-decay models, and whether those differences are explained by family-specific traits related to dispersal ability.
Results
Climatic and spatial distance-decay patterns are very similar in northern Europe, where climatic and spatial distances are highly correlated. In contrast, slopes are steeper in spatial than in climatic distance-decay curves in southern Europe, where climatic and spatial distances are decoupled. Moreover, family traits related to dispersal ability explained the North–South difference in spatial distance-decay slopes, as well as the amount of nestedness-resultant dissimilarity between southern and northern spider faunas.
Main conclusions
Our results suggest that differences in beta diversity patterns between northern and southern Europe reflect the strength of dispersal limitation in spiders, which varies across families and leads to different degrees of disequilibrium with current climatic conditions depending on the taxon. Moreover, in the South of Europe, where spatial and climatic distances are uncorrelated, spatial distance-decay models are steeper and have larger explanatory power than climatic distance-decay models, which suggests that dispersal limitation is the main factor shaping current beta diversity patterns of European spiders at the continental scaleMinisterio de Educación, Cultura y Deporte. Grant Number: FPU17/03016
Ministerio de Ciencia e Innovación. Grant Number: PID2020-116587GB-I00S
Recepción de formatos audiovisuais interculturais Galicia-Portugal emitidos pola TVG: Análise de audiencias en Galicia e na emigración galega da Área Metropolitana de New York
No full textA investigación parte da premisa do acaído, tanto en termos sociais como económicos, dun achegamento da industria audiovisual galega cara os espazos lusófonos, valéndose a tal efecto da capacidade xeradora dun contexto favorable a tal fin a través das canles públicas de televisión, coa conseguinte tentativa de, ao tempo, avanzar na identificación de novos públicos potenciais ao abeiro da incardinación no devandito bloque xeolingüístico
Nervous necrosis virus (NNV) vaccination of carrier Senegalese sole (Solea senegalensis)
Full text linkViral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), is one of the most threatening diseases affecting marine farmed fish, especially in early developmental stages. In addition, subclinical infections are commonly detected in both farmed and wild fish. In recent years numerous efforts have been made to achieve effective vaccines for the protection of different fish species against NNV infection. Vaccination experiments are always conducted on pathogen-free animals, but subclinically infected individuals may respond differently to immunization. In this study, we have observed a different response between experimentally-induced-NNV carrier Senegalese sole and NNV-free animals when immunized with a BEI-inactivated vaccine, whereas mock-vaccinated carrier fish behaved like the NNV-free group. Analysis of the immune response after vaccination showed specific antibody production as well as up-regulation of the genes coding for the proinflammatory cytokines IL-6 and IL-8, the antiviral protein HERC4 and the cytotoxic T lymphocyte (CTL) and T helper markers CD8 and CD4 in vaccinated NNV-free and mock-vaccinated carrier individuals. However, no significant antibody production was detected and only cd4 up-regulation was recorded in the vaccinated carrier fish. After challenge, viral replication was similar in the vaccinated groups up to 15 days post-challenge (dpc), although thereafter the viral load in the vaccinated carrier fish reached values similar to the control fish, whereas in the vaccinated NNV-free sole remained 1.5–2 log lower. At 2 dpc 10% mortality was recorded in the vaccinated carrier group that did not correlate with a higher viral titre, but with an overexpression of inflammation-related genes. These results suggest that an acute inflammatory reaction could have been the cause of the fish death. Excepting the values recorded in this episode, poor modulation of the immune response was observed at the early days post-challenge. However, at 30 dpc the IgM levels in the vaccinated carrier fish were significantly higher than those of the NNV-free sole. Longer post-vaccination studies would be necessary to clarify if this antibody increase may be relevant for fish protectionThis research was funded by Ministerio de Ciencia, Innovación y Universidades (MICIU), the Agencia Estatal de Investigación (AEI) and FEDER, grant number RTI2018-094687-B-C21. Dr. Sandra Souto was funded with a postdoctoral grant from Consellería de Cultura, Educación y Universidad, Xunta de Galicia (postdoctoral grant ED481D-2022/024). The authors are grateful to Stolt Sea Farm for kindly providing the fish, J. Franqueira for technical assistance and Native English School of Languages for the English revisionS
Regulación hipotalámica del balance energético. Papel de AMPKalfa2 y AMPKgamma2
No full textAMPK constituye el principal sensor energético de las células eucariotas, encargándose de
la monitorización del estado energético celular y gobernándolo. A nivel central, también ha
demostrado ser un sensor energético global, teniendo un papel clave a nivel hipotalámico en la
regulación del balance energético. Durante situaciones fisiológicas como una exposición a frío,
la activación de la maquinaria termogénica es clave para la supervivencia. En esta Tesis se
demuestra como la subunidad AMPKα1 en el VMH es clave durante la aclimatación de los
ratones a 4ºC. Evidencias recientes, también apuntan a AMPKα1 en las neuronas SF1 del VMH
como un importante regulador de la termogénesis en el BAT, siendo su inhibición o deleción
capaz de revertir la obesidad inducida por dieta o genética. Sin embargo, el papel de otras
subunidades de AMPK en las neuronas SF1 todavía no se ha descrito. En esta Tesis hemos
estudiado paralelamente el resultado metabólico de la eliminación de AMPKα2 y AMPKγ2 en
neuronas SF1. El modelo SF1-Cre AMPKα2 no presenta alteraciones metabólicas en
condiciones de dieta estándar, pero si un aumento de la masa corporal y adiposidad en
condiciones de HFD, mostrando un fenotipo opuesto al resultado de la eliminación de AMPKα1
en las mismas neuronas. La eliminación de AMPKγ2 en las neuronas SF1 provoca un aumento
de la masa corporal y adiposidad en ambos sexos en condiciones estándar, pero solo en machos
en condiciones de HFD. Estos datos demuestran el papel clave de AMPK y sus diferentes
subunidades en las neuronas SF1 en el control del balance energético.2025-03-1
Deciphering the non-coding genome in Autism Spectrum Disorders (ASD): in silico characterization of common, splicing and regulatory variants
Full text linkThrough a combination of bioinformatics methods and diverse
genetic data collections, we have uncovered insights into
a broad spectrum of genetic variation. In summary, the findings
from this in silico characterization of common, splicing,
and regulatory variants include the identification of specific
genes most likely to be affected by non-coding common
variation, a deeper understanding of how splicing variation may
mediate disease risk, and the establishment of a crucial
role for chromatin conformation in ASD
Aryne‐Based Synthesis of Cyclobutadiene‐Containing Oligoacenes and Related Extended Biphenylene Derivatives
Full text linkA previously undescribed aryne derived from a π-extended biphenylene, 2,3-didehydrobenzo[b]biphenylene, has been developed. The participation of this new aryne building block in [4+2] and palladium-catalyzed [2+2+2] cycloaddition reactions has been applied to the synthesis of a variety of polycyclic conjugated hydrocarbons (PCHs) with appealing structures which combine (aromatic) benzene and (antiaromatic) cyclobutadiene (CBD) rings. Among them, a family of unsubstituted (or barely substituted) CBD-oligoacenes has been accessed by iterative Diels-Alder reactions of the new aryne with furans and/or isobenzofurans, followed by deoxygenative aromatization of the resulting epoxy-derivatives. The experimental and computational studies of the newly synthesized PCHs suggest an important degree of electron delocalization along the polycyclic skeleton, more pronounced in the linearly fused derivatives. The computed ACID plots reveal clockwise current density vectors at the peripheral bonds, originating from the σ contributions of the antiaromatic cyclobutadiene rings.Financial support from grants PID2019-110037GB-I00, PID2022-140845OB-C62 and PID2022-139933NB-I00, funded by MCIN/AEI/10.13039/501100011033, the European Union’s Horizon 2020 (FET-Open project SPRING, Grant No. 863098), and ERC Synergy Grant MOLDAM (951519), the Xunta de Galicia (ED431C 2020/22, ED431H 2020/21 and Centro singular de investigación de Galicia accreditation 2019–2022, ED431G 2019/03) and the European Union (European Regional Development Fund-ERDF) is gratefully acknowledged. The authors thank the Centro de Supercomputación de Galicia (CESGA) for generous allocation of computer time. BA and JJ thank the Agencia Estatal de Investigación for the award of pre-doctoral fellowships (BES-2017-079748 and PRE2020-
092897, respectively)S
Inquiry-based activities with woodlice in Early Childhood Education
Full text linkThis study aims to describe the implementation of a teaching sequence where preschool-age
children participate in activities related with woodlice. Although there is extensive literature on
inquiry-based activities, most studies have been conducted in higher educational stages. Data were
collected though audio and video recordings of the sessions, which have then been transcribed. The
children participated in research activities in which they had no difficulty in posing hypotheses and
making predictions. In relation to the experimental design, we observed difficulties in understanding
the purpose of the experiment and how to reach conclusions from the experimental results. From the
results of this study, we can conclude that children from an early age can engage in inquiry activities
where they are given opportunities to make predictions, formulate hypotheses, and, with the help of
the teacher, plan simple experiments to test their ideasThis research was developed within RODA (Ref. ED431C2021/05) research group and
was funded by the Spanish Ministry of Science, Innovation and Universities, partly funded by the
European Regional Development Fund (ERDF). Grant code PID2022-138166NB-C21S
Alginate aerogels by spray gelation for enhanced pulmonary delivery and solubilization of beclomethasone dipropionate
Full text linkAerogel technology is an emerging platform that offers alternative and cost-effective dry carriers with advanced performances for the pharmaceutical industry. The innovative combination of compressed air-assisted spray gelation and “green” supercritical fluid (SCF) technology was used in this study to produce alginate aerogel microparticles with adequate properties for pulmonary drug delivery. Beclomethasone dipropionate (BDP), a poorly water-soluble anti-inflammatory drug for asthma treatment, was loaded into alginate aerogel particles by SCF-assisted impregnation ensuring high loading, as well as the amorphization of the drug. The production of aerogels was optimized through the fine-tuning of parameters in compressed air-assisted spray gelation, specifically adjusting the air flow rate and the pump speed. Alginate aerogels were aimed to be produced with an appropriate aerodynamic diameter from 1 to 5 µm, measured by in vitro deposition tests with Next Generation impactor and potential for deep lung penetration. Nuclear magnetic resonance (NMR) relaxometry was used to assess aerogel hydration and unveiled structure–property relationships leading to the sudden release of the drug. Finally, in vitro cytotoxicity tests in fibroblasts and ex vivo permeability tests were conducted. These biological tests confirmed the excellent biocompatibility of the aerogel formulations and demonstrated the efficient deposition of BDP in porcine bronchial tissues assisted by the porous alginate aerogel carrierWork supported by MICINN [PID2020-120010RB-I00/AEI/10.13039/501100011033], Xunta de Galicia [ED431C 2020/17], Agencia Estatal de Investigación [AEI] and FEDER funds. This publication is based on work from AERoGELS COST Action (ref. CA18125) supported by COST (European Cooperation in Science and Technology). Th. D. acknowledges the support of Diputación Provincial de A Coruña for research in health science 2022-2023 (BINV-CS/2022) and of AERoGELS COST Action (ref. CA18125) for a Short Term Scientific Missions (STSM) grant, enabling for the collaboration with HUN-REN-DE Mechanisms of Complex Homogeneous and Heterogeneous Chemical Reactions Research Group, Department of Inorganic and Analytical Chemistry, University of Debrecen (Hungary). The authors would like to thank Ezequiel Vázquez Fernández (University of Santiago de Compostela, Spain), Laura Juhász and Krisztián Moldován (University of Debrecen, Hungary) for their valuable support and technical help with Raman spectroscopy, X-ray diffraction, IR-ATR and SEM analysesS