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Los autógrafos teatrales de Luis Vélez de Guevara. Borrón y verso nuevo
No full textEste es el primer estudio de conjunto de los autógrafos dramáticos de Luis Vélez de Guevara. Más allá del análisis particular de cada una de las piezas, se pondrá especial interés en la definición de los elementos comunes y en la identificación de las marcas del proceso de composición, tanto del poeta, como de los censores, autores de comedias e intérpretes que intervienen en ellas. Tras la revisión de las fuentes se descarta la consideración de autógrafos de cinco de las propuestas (Los agravios perdonados, La creación del mundo, La mesa redonda, La Ninfa del cielo y Los novios de Hornachuelos), se deja abierto el debate sobre una (seis páginas de La cristianísima lis) y se confirma la autenticidad de cinco (El águila del agua, la tercera jornada de El catalán Serrallonga, El conde don Pero Vélez, El rey en su imaginación y La serrana de la Vera)
Simple analytical approach to determine the microbiological profile of bee pollen by MALDI-TOF
No full textProducción CientíficaBee pollen is a natural food supplement collected by honeybees that has significant nutritional value and ther-
apeutic qualities, including antioxidant, antibacterial, and anticarcinogenic properties. Due to its hygroscopic
nature, bee pollen readily promotes microbial growth; however, there is no specific legislation for bee pollen in
the European Union. Microbiological contamination therefore stands out as a crucial factor in determining its
quality. To address this, the study focused on isolating bacterial and fungal colonies from bee pollen using
various culture media, followed by a protein extraction based on a lysis process. Identification was achieved
through the Biotyper-MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization coupled to Time-of-Flight de-
tector) system. The greenness and blueness of the methods were assessed using different tools, which classified
them as environmentally friendly and practical. Thirty-two samples were analyzed using proposed sample
treatment approaches. Data were processed using chemometric techniques based on linear models. The results
revealed the presence of both beneficial and potentially pathogenic species, including key representatives such as
Bacillus licheniformis, Micrococcus luteus, and Aspergillus fumigatus. These findings highlight the importance of
microbiota characterization in bee pollen and demonstrate that MALDI-TOF is a rapid and effective tool for
profiling culturable microbial communities in this matrix.Ministerio de Ciencia e Innovación - MICIU/AEI750/10.13039/501100011033 y por FEDER, UE (grant PID2022-141679OR-C33
On the image of a curve in a normal surface by a plane projection
No full textProducción CientíficaWe consider a finite analytic morphism φ = (f, g) defined from a complex analytic normal surface (Z, z) to
C2. We describe the topology of the image by φ of a reduced curve on (Z, z) by means of iterated pencils defined recursively for each branch of the curve from the initial one . This result generalizes the one obtained in a previous paper for the case in which (Z, z) is smooth and the curve irreducible. The methods we use also permit us to describe the topological type of the discriminant curve of φ, in particular, the topological type of each branch of the discriminant can be obtained from the map without previous knowledge of the critical locus.Ministerio de Ciencia e Innovación (MCIN) / Agencia Estatal de Investigación (AEI): PID2022-138906NB-C21 (MCIN/AEI/10.13039/501100011033 / FEDER, EU)Agence Nationale de la Recherche: ANR-17-CE40-0023Open access funding provided by FEDER European Funds and the Junta de Castilla y León under the Research and Innovation Strategy for Smart Specialization (RIS3) of Castilla y León 2021-2027
Hydrothermal pretreatment of brewer's spent grain: A pathway to sustainable biogas production and waste valorization
No full textProducción CientíficaBrewer’s spent grain (BSG), a by-product of the brewing industry, faces significant waste management challenges
but holds potential as a substrate for biogas production via anaerobic digestion (AD). However, its lignocellulosic
complexity limits microbial degradation and methane yield. In previous studies by our research group, various
treatment strategies for BSG were explored, including approaches aimed at recovering high-value compounds.
Building upon this foundation, the present work evaluates the potential of hydrothermal pretreatment to enhance
methane production from BSG via AD. The novelty lies in the combination of a continuous hydrothermal pre-
treatment reactor with meta-transcriptomic analysis of the anaerobic digestion process, enabling a direct cor-
relation between operational performance and microbial functional shifts. Hydrothermal pretreatment was
applied to partially hydrolyze cellulose and hemicellulose into fermentable sugars, improving biodegradability
while minimizing the formation of inhibitory compounds. Results showed that hydrothermally pretreated BSG
supported methane productions over 1500 mL CH4/L_reactor⋅day, markedly higher than the 100–500 mL CH4/
L_reactor⋅day observed for untreated BSG. Volatile solids degradation efficiency improved by 30 %, while
methane content in biogas increased from 30 % to 65 %. Genomic analysis of the microbial consortium revealed
enhanced activity of methanogenic archaea and fermentative bacteria associated with the increased methane
production. This integrated approach not only disrupts lignocellulosic barriers more effectively but also provides
deeper insights into microbial functionality, reinforcing hydrothermal pretreatment as a viable strategy for
boosting biogas yield and advancing sustainable waste-to-energy solutions.BiOBreW project, which is funded by the European Union's Horizon MSCA 2021-PF-01 research and innovation programme, under grant agreement N° 101065428Junta de Castilla y León y del programa FEDER de la UE (UIC 379)Agencia Estatal de Investigación (AEI) (Project TED2021-129837B-C42)Junta de Castilla y León y del programa FEDER de la UE (CLU-2019-04)Ministerio de Ciencia, Innovación y Universidades de España (beca «Beatriz Galindo» BEAGAL18/00247
Circular economy approach to coffee processing residue valorization: Bioactive by-product extracts for managing pre- and post-harvest fungal pathogens
No full textProducción CientíficaThe coffee industry generates a large amount of waste that is usually discarded, creating an environmental and
economic problem. However, these by-products can be a valuable source of bioactive compounds with antimi-
crobial properties and present an opportunity for use in crop protection, either pre- or post-harvest. Following
the principles of the circular economy, this study proposes the extraction and characterization of bioactive
products from coffee by-products, as well as the evaluation of their antifungal activity against pathogens that
affect coffee plants and/or stored coffee beans, such as Fusarium xylarioides, Aspergillus flavus, A. niger, and
Penicillium verrucosum. In vitro activity assays demonstrate high antimicrobial activity of the husk, parchment,
defective green beans with silverskin, and silverskin extracts, with minimum inhibitory concentrations ranging
from 15.6 to 375 μg mL 1 against F. xylarioides, 31.2–1000 μg mL 1 against A. flavus, 62.5–1000 μg mL 1 against
A. niger, and 62.5–1500 μg mL 1 against P. verrucosum, depending on the by-product extract used. The most
effective extract, derived from silverskin, was evaluated for pre-harvest protection of coffee plants and
demonstrated complete inhibition of F. xylarioides-induced tracheomycosis at 15.6 μg mL 1. In turn, a concen-
tration of 62.5 μg mL 1 of the silverskin extract was sufficient to prevent fungal growth of A. flavus, A. niger, and
P. verrucosum on coffee beans. This concentration also prevented mycotoxin production by A. flavus, while a
higher concentration of 125 μg mL 1 was required to prevent aflatoxin production by A. niger. The reported
findings support coffee by-products extracts as promising alternatives to synthetic fungicides, with the potential
to improve the sustainability of the coffee industry.Junta de Castilla y León con cofinanciación FEDER (proyecto VA148P23
Evaluating the experimental CO2 stripping performance of a new generation multicapillary Taylor flow reactor
No full textProducción CientíficaAmong all physicochemical technologies commercially available to upgrade biogas to biomethane, chemical
scrubbing with carbonated solutions stand as an easy-to-scale technology. However, the regeneration of the
solvent via air-assisted CO2 stripping is highly energy-intensive, representing >80 % of the total process costs.
This study proposes the use of innovative Taylor flow reactors to enhance the liquid-gas mass transfer of CO2 and
therefore lower the cost of regeneration of carbonated solutions. In this study, a 200-glass capillary Taylor flow
reactor supporting unprecedented mass transfer coefficients (kLa) was tested for the first time for CO2 stripping
from carbonated solutions. The Taylor flow reactor achieved kLa coefficients higher than 100 h-1 at an inorganic
concentration (IC) of 1000 mgC L-1, and kLa > 400 h-1 at an optimal gas superficial velocity of 0.30 m s-1.
Moreover, increasing IC concentrations to 2000 and 3000 mgC L-1 resulted in a kLa increase of 250 % and 65 %,
respectively, whilst no significant increase was recorded at an initial IC concentration of 4000 mgC L-1. Indeed,
multicapillary Taylor flow reactors demonstrated a superior and competitive performance during CO2 stripping
from carbonated solutions, representing a promising technology for solvent regeneration during biogas
upgrading at industrial scale.Agencia Española de Investigación (PDC2022–133394-I00
Bone marrow-versus adipose tissue-derived mesenchymal stem cells for corneal failure in an experimental model of limbal stem cell deficiency
No full textProducción CientíficaOcular limbal stem cell deficiency (LSCD) occurs because of corneal epithelial stem cell destruction or
dysfunction at the limbal niche. LSCD can cause corneal blindness, and the current therapy based on limbal stem
cell transplantation is continuously improving. The aim of this work was to compare the safety and efficacy of
human mesenchymal stem cells (hMSCs) derived from bone marrow (hBM-MSCs) and adipose tissue (hAT-MSCs)
when transplanted to a rabbit model of LSCD. Both hMSC types expressed the corneal and limbal epithelial cell
markers CK3, CK12, ZO-1, and ABCG2 under standard culture conditions. A few hBM-MSCs expressed CK7 and E-
cadherin, while hAT-MSCs expressed more CK7 but no E-cadherin. The hMSCs were seeded onto amniotic
membranes and transplanted onto the ocular surface of a LSCD rabbit model. Both hMSC types were well
tolerated without immunosuppression and were primarily located in the superior limbal stroma eight weeks post-
transplantation. The hBM-MSC–treated group showed less superficial neovascularization, while the hAT-
MSC–treated group showed less conjunctival invasion and fewer corneal stromal blood vessels. Compared to the
untreated LSCD group, both hMSC-treated groups had less corneal opacity, less corneal and limbal stromal
inflammation, and more corneal epithelial layers that partially recovered the corneal and limbal epithelial
markers CK3, CK15, and p63. Overall, transplantation of hBM-MSCs and hAT-MSCs in a rabbit LSCD model
reduced the development of corneal opacity, neovascularization, inflammation, and partially restored corneal
and limbal tissue structure and epithelial cell phenotypes. Therefore, both types of hMSCs could become valid
alternatives for LSCD treatment.Ministerio de Economía y Competitividad y el Fondo Europeo de Desarrollo Regional, España (subvención SAF2015-63594-R MINECO/FEDER, UE)Ministerio de Ciencia e Innovación (subvención PID2019-105525RB-100 AEI/10.13039/501100011033)Instituto Nacional de Salud Carlos III, CIBER-BBN (CB06/01/003 MICINN/FEDER, UE)Junta de Castilla y León (Consejería de Educación) y el Fondo Europeo de Desarrollo Regional (subvención CLU-2023-1-04
Updated research data for "Static structure, microscopic dynamics and electronic properties of the Fe-Ni and Fe-Ni-S liquid alloys. An ab initio molecular dynamics study."
No full textResearch data corresponding to the paper "Static structure, microscopic dynamics and electronic properties of the Fe-Ni and Fe-Ni-S liquid alloys. An ab initio molecular dynamics study." submitted to Journal of Physics: Condensed Matter for publication.Departamento de Física Teórica, Atómica y ÓpticaGIR Propiedades Nanométricas de la Materi
Sistema modular de almacenamiento y motorización de la calidad del aire en Castilla y León
No full textDesarrollo e implementación de un sistema modular de almacenamiento y monitorización de la calidad del aire en Castilla y León. El sistema esta
construido a partir de Docker, utilizando herramientas como Apache Nifi, MinIO, InfluxDB y Grafana. Mediante los servicios anteriores se captura,
transforma, almacena, organiza y visualiza la información necesaria para la comprensión y estudio de la calidad del aire.
La solución puede ser ejecutada desde un único comando Docker, levantando cada uno de los contenedores independientes que contienen todo lo
necesario para el funcionamiento del total de las partes. El resultado final del proceso ETL quedará plasmado en múltiples paneles gráficos. Cuenta
también con un entorno centralizado de almacenamiento tipo Datalake.
El sistema dada su arquitectura basada en componentes es modular, sencillo de desplegar y muy escalable.Grado en Ingeniería Informática y de Servicios y Aplicacione
Creative narratives in the age of Artificial Intelligence
No full textProducción CientíficaEl presente trabajo expone un diagnóstico inicial del uso de las herramientas de inteligencia artificial (IA) en las aulas de los centros de educación primaria (EP) de Castilla y León, observando desde el conocimiento e interés de los docentes por las mismas, hasta la implementación de una intervención con IA, pasando por la consideración de los valores didácticos y la valoración del potencial del fomento del espíritu crítico en dicho alumnado. Tanto el estudio como la propuesta de intervención se diseñan desde una perspectiva interdisciplinar, con el trabajo de las habilidades comunicativas como eje principal. El estudio se lleva a cabo en varias fases para tener una visión más completa, tanto del alumnado que las aplica en su actividad, como del profesorado: su acceso a formación, su experiencia personal, sus propias habilidades de uso y una reflexión crítica orientada a la aplicación de estas herramientas para la mejora de las habilidades comunicativas del alumnado.This study offers an initial diagnosis of the integration of artificial intelligence (AI) tools in Primary Education classrooms in Castilla y León (Spain). It examines teachers’ levels of awareness, interest, and training, as well as their own experiences and practical skills in employing such tools. The analysis extends to the implementation of an AI-based proposal of intervention, with particular attention to the didactic value of these technologies and their potential to foster students’ critical thinking. Both the research and the intervention design adopt an interdisciplinary perspective, focusing on the development of communicative skills. The study is structured in various phases (from a survey study with teachers and school management teams) to provide a comprehensive perspective that encompasses not only students’ engagement with AI in learning activities, but also teachers’ access to professional development, personal experiences, and critical reflections on the pedagogical use of AI for enhancing students’ communicative competences