50107 research outputs found
Sort by
Reporter-Mediated Evaluation of the Circadian Oscillations of SNAIL Across In Vitro Models
No full textThe protein SNAIL has been widely studied for its roles in promoting cancer invasion and resistance to apoptosis. There are multiple contributors to its expression, including self- and circadian regulation, and it has been posited that SNAIL oscillates in a circadian manner. Given the multiple factors involved, we sought to determine whether this is indeed the case. We developed a luciferase reporter that was used to demonstrate SNAIL’s rhythmic nature (SNAIL:luc) in the circadian model cell line, U2OS. Considering SNAIL’s relevance in breast cancer, we also assessed its oscillations in cellular models representing different levels of aggression. We incorporated the SNAIL:luc reporter in MCF10A breast epithelial cells, and MCF7 and MDA-MB-231 breast cancer cell lines, which are less and more aggressive, respectively. We found that SNAIL oscillations were present but weak in MCF7 and arrhythmic in MDA-MB-231 cells, correlating with those of core clock genes in these models. Surprisingly, MCF10A cells, whose core clock genes possess robust circadian expression patterns, did not have rhythmic oscillations of SNAIL. Our findings suggest that SNAIL is under circadian control and sets the stage for additional studies to better understand the impacts of various factors contributing to its expression
Low-Carbon Climate Resilience Opportunities in Small Limited Resource Cities
No full textAs many communities pursue low-carbon, climate-resilient futures, medium to large cities have often taken the lead, supported by organizations like the C40 Network, Bloomberg Philanthropies, and the former Rockefeller 100 Resilient Cities initiative. However, smaller communities, especially those with median or below-median income levels, frequently lack access to these resources and opportunities. This gap is significant, as more Americans reside in smaller cities and communities than in large cities. This report examines the unique opportunities and challenges faced by smaller cities, particularly those with limited financial resources and specialized staff.
This case study explores how Northampton, Massachusetts—a small city with a population of just under 30,000—has navigated these challenges by setting ambitious climate targets, aligning its planning and capital improvement efforts with sustainability and resilience goals, and establishing a dedicated climate action department. Northampton's achievements, which include the highest score in the former Massachusetts Commonwealth Capital sustainability program, the first 5-STAR rating in the Sustainability Tools for Assessing and Rating Communities, and a LEED for Cities Gold certification, provide valuable insights into how small, median-income cities can make meaningful progress on climate change. The lessons learned from Northampton’s successes and setbacks offer actionable guidance for other smaller communities aiming to address climate resilience and sustainability.Lincoln Institute for Land Policy
Association of Collegiate Schools of Plannin
Reducing carbon emissions from hotel room air conditioner use – Closing the attitude-behavior gap
No full textOne of the main sources of carbon emissions generated by hotel operations are in-room air conditioners used for both heating and cooling. This study tests several message-based approaches that leverage promising latent theoretical constructs (e.g., social norms, compassion, perceived anthropomorphism, and humor) to encourage hotel guests to set the air conditioner in a specific range, which reduces emissions without compromising guest experience in both winter and summer. Several messages are effective in triggering the targeted theoretical constructs, with eight (4 in winter and 4 in summer) messages also translating into sustainable air conditioner use. This study demonstrates how simple and cheap messages can influence behavior and substantially reduce hotel-generated carbon emissions; a change in 1 degree can reduce emission by up to 6%
Should I stay or should I go? Place detachment in the face of overtourism
No full textOvertourism has become a major issue worldwide and will remain a key issue for local residents, tourism actors and local authorities in years to come. Whilst academics have researched tourism impacts for decades, overtourism is more complex and its various consequences have been met by a fierce opposition from local residents in various destinations. This paper aims to revisit older models of tourism impacts to provide a more detailed approach of the overtourism stages and its associated consequences. It details the shock wave that overtourism represents for local populations and how this process gradually pushes them away from their place of daily living. The research also investigates residents who have made the conscious choice to move away from their destination, explaining how place detachment occurs and its main causes
More Negative, More Memorable: The Role of Social Sharing of Negative Emotions in Shaping Dark Tourism Experiences
No full textVisiting dark tourism sites—destinations associated with death and suffering— often exposes tourists to emotional conditions that trigger Social Sharing of Emotions (SSE). This paper explores how sharing negative emotions influences tourists' emotional experiences in dark tourism contexts. Study 1 shows that sharing negative emotions intensifies the sharer's emotions, making the experience more memorable. Study 2 reveals that recipient feedback moderates this effect, while Study 3 finds that the impact varies depending on the sharing mode and the relationship between the sharer and recipient. These findings challenge the ‘emotional venting’ belief and suggest that sharing with strong ties is not always more beneficial than sharing with weak ties. From a managerial perspective, encouraging social sharing enhances emotional engagement and create deeper, lasting memories. Operators should create spaces for emotional expression and tailor sharing strategies based on visitors’ relationship dynamics
From Memories to Intentions: How Memorable Travel Experiences Enhance Well-being and Drive Future Travel
No full textThis study investigates how memorable travel experiences (MTE) influence tourists' emotions, psychological well-being, and future travel intentions, also testing for the potential mediating effects within these relationships. Survey data from 400 Korean participants reveal that MTEs positively influence positive emotions and negatively impact negative emotions, both of which affect PWB. PWB, in turn, is a significant predictor of future travel intentions. Structural Equation Modeling results confirm that positive emotions mediate the relationship between MTEs and PWB, while negative emotions also show partial mediation effects. These findings offer insights into designing tourism experiences that foster well-being, supporting sustainability and growth in the tourism industry
Sustainable Skiing - How do locals and tourists perceive sustainable development of ski tourism?
No full textGlobal warming poses major challenges for Alpine tourism and ski resorts in particular. Consequences such as shifts of seasons or technical snowmaking raise doubts about the sustainability of the industry. This study investigates the perceived economic, ecological, and socio-cultural impacts of ski tourism on the residents’ and tourist support for tourism development. The perception theory serves as a theoretical basis for this study. Following a quantitative research design, 340 local and tourist skiers in a major Tyrolean glacier ski resort were interviewed using an online survey. The results reveal that perceived ecological and socio-cultural benefits of sustainability positively affect the support for tourism development. This study could not confirm any significant differences between the perceptions of locals and tourists. The study's findings provide valuable insights into how ski resorts can more effectively communicate their sustainability efforts to locals and tourists
Exploring cross-cultural perceptions of craft beer: employing Q methodology in tourism research
No full textThe burgeoning interest in craft beer represents a dynamic aspect of the global tourism landscape, offering rich opportunities for cultural exchange and endogenous economic development. While the craft beer movement is well-established in Western contexts, its emergence in China reflects broader patterns of globalization and cultural adaptation. This qualitative methods workshop will discuss the strengths and limitations of using Q methodology to explore and compare the meanings attributed to craft beer by Chinese and Western consumers. By delving into the subjective perceptions of these groups, Q methodology appears particularly well suited to unveil the cultural nuances and consumer motivations that influence the craft beer sector and consequently bear strong implications for the beer tourism segment and destinations’ competitiveness
Exploring engagement, social marketing, and destination decision making to reduce health risks in coastal waters: a systematic review with insights from Ireland and New England, USA
No full textOcean based recreation is a major community asset for local residents and visitors alike. Individuals seek out coastal beaches for swimming, boating, fishing, surfing, and wildlife viewing. Water quality can be a key variable that facilitates ocean recreation. The associated economic and social benefits gained for both individuals and communities through coastal recreation can be impacted positively and negatively by water quality and individual decision making processes. We are undertaking a systematic review of peer reviewed studies combined with grey literature of community case studies and insights from both New England and Ireland. The systematic review will provide important insight into decision making processes that both residents and visitors work through when they are choosing destinations to visit and spend their time and money. Water quality monitoring and decision making are important topics for tourism professionals, especially in coastal communities, and we hope this discussion provides food for thought
Segmenting the Audience for Social Marketing Campaigns: Case of Orphanage Tourism
No full textThis study conducted a Twitter audience segmentation to enhance social marketing campaigns by applying topic modeling, keyword matching, and geo-location mapping. Using orphanage tourism as a case study, the study analyzed Twitter users' profiles and posts to extract demographic, geographic, and psychographic information. The findings identified commonalities among the target audience, segmenting them into homogeneous subgroups. Results revealed audience segments that were both novel and consistent with past studies, validating the method's applicability. Key segments for anti-orphanage tourism campaigns included Christians, adults, teachers, and university students, with geographic hotspots in South Africa, the United States, and the United Kingdom. This study offers practical value for governments and non-profit organizations by enabling the development of cost-effective, targeted social media campaigns