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Tracing long-distance electron transfer and cable bacteria in freshwater sediments by agar pillar gradient columns.
No full textCable bacteria (CB) perform electrogenic sulphur oxidation (e-SOX) by spatially separating redox-half-reactions over cm-distances. For freshwater systems, the ecology of CB is not yet well understood, partly because they proved difficult to cultivate. This study introduces a new "agar pillar" approach to selectively enrich and investigate CB-populations. Within sediment columns, a central agar pillar is embedded, providing a sediment-free gradient-system in equilibrium with the surrounding sediment. We incubated freshwater sediments from a streambed, a sulfidic lake, and a hydrocarbon polluted aquifer in such agar pillar columns. Microprofiling revealed typical patterns of e-SOx, such as the development of a suboxic zone and the establishment of electric potentials. The bacterial communities in the sediments and agar pillars were analysed over depth by PacBio near-full-length 16S rRNA gene amplicon sequencing, allowing for a precise phylogenetic placement of taxa detected. The selective niche of the agar pillar was preferentially colonized by CB related to Candidatus Electronema for surface-water sediments, including several potentially novel species, but not for putative groundwater CB affiliated with Desulfurivibrio spp. The presence of CB was seemingly linked to co-enriched fermenters, hinting at a possible role of e-SOx-populations as an electron sink for heterotrophic microbes. These findings add to our current understanding of the diversity and ecology of CB in freshwater systems, and to a discrimination of CB from surface and groundwater sediments. The agar pillar approach provides a new strategy that may facilitate the cultivation of redox gradient-dependent microorganisms, including previously unrecognized CB populations
Heterogeneous nature of the carbonaceous chondrite breccia Aguas Zarcas – Cosmochemical characterization and origin of new carbonaceous chondrite lithologies.
No full textOn April 23rd, 2019, the Aguas Zarcas meteorite fall occurred in Costa Rica. Because the meteorite was quickly recovered, it contains valuable extraterrestrial materials that have not been contaminated by terrestrial processes. Our X-ray computed tomography (XCT) and scanning electron microscopy (SEM) results on various pre-rain fragments from earlier work (Kerraouch et al., 2020; 2021) revealed several distinct lithologies: Two distinct metal-rich lithologies (Met-1 and Met-2), a CM1/2 lithology, a C1 lithology, and a brecciated CM2 lithology consisting of different petrologic types. Here, we further examined these lithologies in the brecciated Aguas Zarcas meteorite and report new detailed mineralogical, chemical, isotopic, and organic matter characteristics. In addition to petrographic differences, the lithologies also display different chemical and isotopic compositions. The variations in their bulk oxygen isotopic compositions indicate that the various lithologies formed in different environments and/or under diverse conditions (e.g., water/rock ratios). Each lithology experienced a different hydration period during its evolution. Together, this suggests that multiple precursor parent bodies may have been involved in these processes of impact brecciation, mixing, and re-assembly. The Cr and Ti isotopic data for both the CM1/2 and Met-1 lithology are consistent with those of other CM chondrites, even though Met-1 displays a significantly lower ε50Ti isotopic composition that may be attributable to sample heterogeneities on the bulk meteorite scale and may reflect variable abundances of refractory phases in the different lithologies of Aguas Zarcas. Finally, examination of the organic matter of the various lithologies also suggests no strong evidence of thermal events, but a short-term heating cannot completely be excluded. Raman parameters indicate that the peak temperature has been lower than that for Yamato-793321 (CM2, ∼400 °C). Considering the new information presented in this study, we now better understand the origin and formation history of the Aguas Zarcas daughter body
Short-term effects of cold spells on plasma viscosity: Results from the KORA cohort study in Augsburg, Germany.
No full textAs the underlying mechanisms of the adverse effects of cold spells on cardiac events are not well understood, we explored the effects of cold spells on plasma viscosity, a blood parameter linked to cardiovascular disease. This cross-sectional study involved 3622 participants from the KORA S1 Study (1984-1985), performed in Augsburg, Germany. Exposure data was obtained from the Bavarian State Office for the Environment. Cold spells were defined as two or more consecutive days with daily mean temperatures below the 3rd, 5th, or 10th percentile of the distribution. The effects of cold spells on plasma viscosity were explored by generalized additive models with distributed lag nonlinear models (DLNM). We estimated cumulative effects at lags 0-1, 0-6, 0-13, 0-20, and 0-27 days separately. Cold spells (mean temperature <3rd, <5th or <10th percentile) were significantly associated with an increase in plasma viscosity with a lag of 0-1 days [%change of geometric mean (95% confidence interval): 1.35 (0.06-2.68), 1.35 (0.06-2.68), and 2.49 (0.34-4.69), respectively], and a lag of 0-27 days [18.81 (8.97-29.54), 17.85 (8.29-28.25), and 7.41 (3.35-11.0), respectively]. For the analysis with mean temperature <3rd or 10th percentile, we also observed significant associations at lag 0-20 days [8.34 (0.43-16.88), and 4.96 (1.68, 8.35), respectively]. We found that cold spells had significant immediate and longer lagged effects on plasma viscosity. This finding supports the complex interplay of multiple mechanisms of cold on adverse cardiac events and enriches the knowledge about how cold exposure acts on the human body
Interplay of the human exposome, metabolome and gut microbiome in dementia and major depression.
No full textBackground: The pathogenesis of dementia and depression is complex involving the interplay of genetic and environmental risk factors including diet, life-style and the gut microbiome. Dementia and depression co-occur and metabolomics studies may shed light on the interplay of the various risk factors. Methods: We have studied the metabolome of 118,466 individuals including 8462 cases with a history of major depression (MDD) and 1,364 patients who developed dementia during follow-up from the UK Biobank (UKB). The human metabolome was profiled using the Nightingale platform. Result: For both disorders, we find direct evidence that metabolites involved in the tricarboxylic acid (TCA) cycle are altered in patients, albeit that different metabolites emerge as the most significant drivers in the two disorders. Both dementia and MDD dementia patients show a marked change in the HDL/VLDL axis in blood, with similar changes in particular small and extra large HDL subfractions seen in patients with MDD and those who develop depression in the future. The two patients groups further show similar changes in fat metabolism as measured by omega 3, omega 6 and PUFA levels. When comparing metabolic profiles over environmental risk factors for MDD and dementia, we find that MDD clusters with dementia risk factors physical activity, history of previous smoking and social isolation. Integrating the metabolic profiles of major depression and the gut microbiome we find that the gut microbiome may be a key mediator in the relationship between various metabolites involved in the HDL subfractions associated to both MDD and dementia. Conclusion: Our study shows that energy and fat metabolism is disturbed in patients with MDD as well as patients who develop dementia in the future and that the interplay between the genome, exposome, gut microbiome, human metabolome may play role in the co-occurrence of major depression and dementia
Kidney tubular epithelial cell ferroptosis links glomerular injury to tubulointerstitial pathology in lupus nephritis.
No full textFerroptosis is a druggable, iron-dependent form of cell death that is characterized by lipid peroxidation but has received little attention in lupus nephritis. Kidneys of lupus nephritis patients and mice showed increased lipid peroxidation mainly in the tubular segments and an increase in Acyl-CoA synthetase long-chain family member 4, a pro-ferroptosis enzyme. Nephritic mice had an attenuated expression of SLC7A11, a cystine importer, an impaired glutathione synthesis pathway, and low expression of glutathione peroxidase 4, a ferroptosis inhibitor. Lipidomics of nephritic kidneys confirmed ferroptosis. Using nephrotoxic serum, we induced immune complex glomerulonephritis in congenic mice and demonstrate that impaired iron sequestration within the proximal tubules exacerbates ferroptosis. Lupus nephritis patient serum rendered human proximal tubular cells susceptibility to ferroptosis which was inhibited by Liproxstatin-2, a novel ferroptosis inhibitor. Collectively, our findings identify intra-renal ferroptosis as a pathological feature and contributor to tubular injury in human and murine lupus nephritis
Health, pleasure, and fullness: changing mindset affects brain responses and portion size selection in adults with overweight and obesity.
No full textBackground Increased portion size is an essential contributor to the current obesity epidemic. The decision of how much to eat before a meal begins (i.e. pre-meal planning), and the attention assigned to this task, plays a vital role in our portion control. Objective We investigated whether pre-meal planning can be influenced by a shift in mindset in individuals with overweight and obesity in order to influence portion size selection and brain activity. Design We investigated the neural underpinnings of pre-meal planning in 36 adults of different weight groups (BMI < 25 kg/m(2) and BMI >= 25 kg/m(2)) by means of functional magnetic resonance imaging. To examine the important role of attentional focus, participants were instructed to focus their mindset on the health effects of food, expected pleasure, or their intention to stay full until dinnertime, while choosing their portion size for lunch. Results We observed that participants of all weight groups reduced their portion size when adopting a health mindset, which was accompanied by enhanced activation of the self-control network (i.e. left prefrontal cortex). Fullness and pleasure mindsets resulted in contrasting reward responses in individuals with overweight and obesity compared to normal-weight individuals. Under the pleasure mindset, persons with overweight and obesity showed heightened activity in parts of the taste cortex (i.e. right frontal operculum), while the fullness mindset caused reduced activation in the ventral striatum, an important component of the reward system. Moreover, participants with overweight and obesity did not modify their behaviour under the pleasure mindset and selected larger portions than the normal-weight group. Conclusions We were able to identify specific brain response patterns as participants made a final choice of a portion size. The results demonstrate that different brain responses and behaviours during pre-meal planning can inform the development of effective strategies for healthy weight management
On the robustness of noise-blind low-rank recovery from rank-one measurements.
No full textWe prove new results about the robustness of well-known convex noise-blind optimization formulations for the reconstruction of low-rank matrices from an underdetermined system of random linear measurements. Specifically, our results address random Hermitian rank-one measurements as used in a version of the phase retrieval problem; that is, each measurement can be represented as the inner product of the unknown matrix and the outer product of a given realization of the standard complex Gaussian random vector. We obtain our results by establishing that with high probability the measurement operator consisting of independent realizations of such a random rank-one matrix exhibits the so-called Schatten-1 quotient property, which corresponds to a lower bound for the inradius of their image of the nuclear norm (Schatten-1) unit ball. We complement our analysis by numerical experiments comparing the solutions of noise-blind and noise-aware formulations. These experiments confirm that noise-blind optimization methods exhibit comparable robustness to noise-aware formulations
Aerosol emissions from a marine diesel engine running on different fuels and effects of exhaust gas cleaning measures.
No full textThe emissions of marine diesel engines have gained both global and regional attentions because of their impact on human health and climate change. To reduce ship emissions, the International Maritime Organization capped the fuel sulfur content of marine fuels. Consequently, either low-sulfur fuels or additional exhaust gas cleaning devices for the reduction in sulfur dioxide (SO2) emissions became mandatory. Although a wet scrubber reduces the amount of SO2 significantly, there is still a need to consider the reduction in particle emissions directly. We present data on the particle removal efficiency of a scrubber regarding particle number and mass concentration with different marine fuel types, marine gas oil, and two heavy fuel oils (HFOs). An open-loop sulfur scrubber was installed in the exhaust line of a marine diesel test engine. Fine particulate matter was comprehensively characterized in terms of its physical and chemical properties. The wet scrubber led up to a 40% reduction in particle number, whereas a reduction in particle mass emissions was not generally determined. We observed a shift in the size distribution by the scrubber to larger particle diameters when the engine was operated on conventional HFOs. The reduction in particle number concentrations and shift in particle size were caused by the coagulation of soot particles and formation/growing of sulfur-containing particles. Combining the scrubber with a wet electrostatic precipitator as an additional abatement system showed a reduction in particle number and mass emission factors by >98%. Therefore, the application of a wet scrubber for the after-treatment of marine fuel oil combustion will reduce SO2 emissions, but it does not substantially affect the number and mass concentration of respirable particulate matters. To reduce particle emission, the scrubber should be combined with additional abatement systems
The role of DNA methylation and histone modifications in blood pressure: A systematic review.
No full textEpigenetic mechanisms might play a role in the pathophysiology of hypertension, a major risk factor for cardiovascular disease and renal failure. We aimed to systematically review studies investigating the association between epigenetic marks (global, candidate-gene or genome-wide methylation of DNA, and histone modifications) and blood pressure or hypertension. Five bibliographic databases were searched until the 7th of December 2018. Of 2984 identified references, 26 articles based on 25 unique studies met our inclusion criteria, which involved a total of 28,382 participants. The five studies that assessed global DNA methylation generally found lower methylation levels with higher systolic blood pressure, diastolic blood pressure, and/or presence of hypertension. Eighteen candidate-gene studies reported, in total, 16 differentially methylated genes, including renin-angiotensin-system-related genes (ACE promoter and AGTR1) and genes involved in sodium homeostasis and extracellular fluid volume maintenance system (NET promoter, SCNN1A, and ADD1). Between the three identified epigenome-wide association studies (EWAS), lower methylation levels of SULF1, EHMT2, and SKOR2 were found in hypertensive patients as compared with normotensive subjects, and lower methylation levels of PHGDH, SLC7A11, and TSPAN2 were associated with higher systolic and diastolic blood pressure. In summary, the most convincing evidence has been reported from candidate-gene studies, which show reproducible epigenetic changes in the interconnected renin-angiotensin and inflammatory systems. Our study highlights gaps in the literature on the role of histone modifications in blood pressure and the need to conduct high-quality studies, in particular, hypothesis-generating studies that may help to elucidate new molecular mechanisms
Design and characterization of dietary assessment in the German National Cohort.
No full textBackground/Objectives The aim of the study was to describe a novel dietary assessment strategy based on two instruments complemented by information from an external population applied to estimate usual food intake in the large-scale multicenter German National Cohort (GNC). As proof of concept, we applied the assessment strategy to data from a pretest study (2012-2013) to assess the feasibility of the novel assessment strategy.Subjects/Methods First, the consumption probability for each individual was modeled using three 24 h food lists (24h-FLs) and frequencies from one food frequency questionnaire (FFQ). Second, daily consumed food amounts were estimated from the representative German National Nutrition Survey II (NVS II) taking the characteristics of the participants into account. Usual food intake was estimated using the product of consumption probability and amounts.Results We estimated usual intake of 41 food groups in 318 men and 377 women. The participation proportion was 100, 84.4, and 68.5% for the first, second, and third 24h-FL, respectively. We observed no associations between the probability of participating and lifestyle factors. The estimated distributions of usual food intakes were plausible and total energy was estimated to be 2707 kcal/day for men and 2103 kcal/day for women. The estimated consumption frequencies did not differ substantially between men and women with only few exceptions. The differences in energy intake between men and women were mostly due to differences in estimated daily amounts.Conclusions The combination of repeated 24h-FLs, a FFQ, and consumption-day amounts from a reference population represents a user-friendly dietary assessment approach having generated plausible, but not yet validated, food intake values in the pretest study