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Phytoplasma sap11 effector destabilization of tcp transcription factors differentially impact development and defence of Arabidopsis versus maize
No full textAuthor summary Phytoplasmas are parasites of a wide range of plant species and are transmitted by sap-feeding insects, such as leafhoppers. Phytoplasma-infected plants are often easily recognized because of their dramatic symptoms, including shoot proliferations (witch's brooms) and altered leaf shapes, leading to severe economic losses of crops, ornamentals and trees worldwide. We previously found that the virulence protein SAP11 of aster yellows witches' broom phytoplasma (AY-WB) interferes with a specific group of plant transcription factors, named TCPs, leading to witches' brooms and leaf shape changes of the model plant Arabidopsis thaliana. SAP11 has been characterized in a number of other phytoplasmas. However, it is not known how phytoplasmas and their SAP11 proteins modulate processes in crops, including cereals such as maize. We identified a SAP11 homolog in Maize bushy stunt phytoplasma (MBSP), a pathogen that can cause severe yield losses of maize. We found that SAP11 interactions with TCPs are conserved between maize and Arabidopsis, and that MBSP SAP11 interferes with less TCPs compared to AY-WB SAP11. This work provides new insights into how phytoplasmas change maize architecture and corn production. Moreover, we found that TCPs regulate leaf defence responses to phytoplasma leafhopper vectors in Arabidopsis, but not in maize. Phytoplasmas are insect-transmitted bacterial pathogens that colonize a wide range of plant species, including vegetable and cereal crops, and herbaceous and woody ornamentals. Phytoplasma-infected plants often show dramatic symptoms, including proliferation of shoots (witch's brooms), changes in leaf shapes and production of green sterile flowers (phyllody). Aster Yellows phytoplasma Witches' Broom (AY-WB) infects dicots and its effector, secreted AYWB protein 11 (SAP11), was shown to be responsible for the induction of shoot proliferation and leaf shape changes of plants. SAP11 acts by destabilizing TEOSINTE BRANCHED 1-CYCLOIDEA-PROLIFERATING CELL FACTOR (TCP) transcription factors, particularly the class II TCPs of the CYCLOIDEA/TEOSINTE BRANCHED 1 (CYC/TB1) and CINCINNATA (CIN)-TCP clades. SAP11 homologs are also present in phytoplasmas that cause economic yield losses in monocot crops, such as maize, wheat and coconut. Here we show that a SAP11 homolog of Maize Bushy Stunt Phytoplasma (MBSP), which has a range primarily restricted to maize, destabilizes specifically TB1/CYC TCPs. SAP11(MBSP) and SAP11(AYWB) both induce axillary branching and SAP11(AYWB) also alters leaf development of Arabidopsis thaliana and maize. However, only in maize, SAP11(MBSP) prevents female inflorescence development, phenocopying maize tb1 lines, whereas SAP11(AYWB) prevents male inflorescence development and induces feminization of tassels. SAP11(AYWB) promotes fecundity of the AY-WB leafhopper vector on A. thaliana and modulates the expression of A. thaliana leaf defence response genes that are induced by this leafhopper, in contrast to SAP11(MBSP). Neither of the SAP11 effectors promote fecundity of AY-WB and MBSP leafhopper vectors on maize. These data provide evidence that class II TCPs have overlapping but also distinct roles in regulating development and defence in a dicot and a monocot plant species that is likely to shape SAP11 effector evolution depending on the phytoplasma host range
New approach studying interactions regarding trade-off between beef performances and meat qualities
No full textThe beef cattle industry is facing multiple problems, from the unequal distribution of
added value to the poor matching of its product with fast-changing demand. Therefore, the aim of
this study was to examine the interactions between the main variables, evaluating the nutritional
and organoleptic properties of meat and cattle performances, including carcass properties, to assess
a new method of managing the trade-off between these four performance goals. For this purpose,
each variable evaluating the parameters of interest has been statistically modeled and based on data
collected on 30 Blonde d’Aquitaine heifers. The variables were obtained after a statistical pretreatment
(clustering of variables) to reduce the redundancy of the 62 initial variables. The
sensitivity analysis evaluated the importance of each independent variable in the models, and a
graphical approach completed the analysis of the relationships between the variables. Then, the
models were used to generate virtual animals and study the relationships between the nutritional
and organoleptic quality. No apparent link between the nutritional and organoleptic properties of
meat (r = −0.17) was established, indicating that no important trade-off between these two qualities
was needed. The 30 best and worst profiles were selected based on nutritional and organoleptic
expectations set by a group of experts from the INRA (French National Institute for Agricultural
Research) and Institut de l’Elevage (French Livestock Institute). The comparison between the two
extreme profiles showed that heavier and fatter carcasses led to low nutritional and organoleptic
quality
Imaging spectroscopy for soil mapping and monitoring
No full textThere is a renewed awareness of the finite nature of the world's soil resources, growing concern about soil security and significant uncertainties about the carrying capacity of the planet. Regular assessments of soil conditions from local through to global scales are requested, and there is a clear demand for accurate, up-to-date and spatially referenced soil information by the modelling scientific community, farmers and land users, and policy- and decision-makers. Soil and imaging spectroscopy, based on visible-near-infrared and shortwave infrared (400-2500nm) spectral reflectance, has been shown to be a proven method for the quantitative prediction of key soil surface properties. With the upcoming launch of the next generation of hyperspectral satellite sensors in the next years, a high potential to meet the demand for global soil mapping and monitoring is appearing. In this paper, we briefly review the basic concepts of soil spectroscopy with a special attention to the effects of soil roughness on reflectance and then provide a review of state of the art, achievements and perspectives in soil mapping and monitoring based on imaging spectroscopy from air- and spaceborne sensors. Selected application cases are presented for the modelling of soil organic carbon, mineralogical composition, topsoil water content and characterization of soil crust, soil erosion and soil degradation stages based on airborne and simulated spaceborne imaging spectroscopy data. Further, current challenges, gaps and new directions toward enhanced soil properties modelling are presented. Overall, this paper highlights the potential and limitations of multiscale imaging spectroscopy nowadays for soil mapping and monitoring, and capabilities and requirements of upcoming spaceborne sensors as support for a more informed and sustainable use of our world's soil resources
Blockade of IL-33R/ST2 signaling attenuates Toxoplasma gondii Ileitis depending on IL-22 expression
No full textOral T. gondii infection (30 cysts of 76K strain) induces acute lethal ileitis in sensitive C57BL/6 (B6) mice with increased expression of IL-33 and its receptor ST2 in the ileum. Here we show that IL-33 is involved in ileitis, since absence of IL-33R/ST2 attenuated neutrophilic inflammation and Th1 cytokines upon T. gondii infection with enhanced survival. Blockade of ST2 by neutralizing ST2 antibody in B6 mice conferred partial protection, while rmIL-33 aggravated ileitis. Since IL-22 expression further increased in absence of ST2, we blocked IL-22 by neutralizing antibody, which abrogated protection from acute ileitis in ST2 deficient mice. In conclusion, severe lethal ileitis induced by oral T. gondii infection is attenuated by blockade of ST2 signaling and may be mediated in part by endogenous IL-22
Milk fatty acids can predict enteric methane emissions from dairy cows fed various diets
No full textCoupling water fluxes with cell wall mechanics in a multicellular model of plant development
No full textThe growth of plant organs is a complex process powered by osmosis that attracts water inside the cells; this influx induces simultaneously an elastic extension of the walls and pressure in the cells, called turgor pressure; above a threshold, the walls yield and the cells grow. Based on Lockhart’s seminal work, various models of plant morphogenesis have been proposed, either for single cells, or focusing on the wall mechanical properties. However, the synergistic coupling of fluxes and wall mechanics has not yet been fully addressed in a multicellular model. This work lays the foundations of such a model, by simplifying as much as possible each process and putting emphasis on the coupling itself. Its emergent properties are rich and can help to understand plant morphogenesis. In particular, we show that the model can display a new type of lateral inhibitory mechanism that amplifies growth heterogeneities due e.g to cell wall loosening
Acidity drop and coloration in clementine: implications for fruit quality and harvesting practices
No full textThe commercial quality of fruit is the result of a combination of internal (acidity, sugars, juice, etc.) and external characteristics (shape, size, color, visual defects, etc.). On citrus, the internal maturity of fruit is often reached prior and independently to their external maturity, inducing the use of degreening practices to artificially color fruit. However, for some sectors where degreening is not authorized, such as organic farming or upmarket, it is important to understand the co-occurrence between fruit coloration and internal ripening, and its impact on fruit quality and harvesting management. Our study was based on a monitoring of the color and acidity of Protected Geographical Indication “Clémentine de Corse” orchards of producers in 2013 and 2014. Our results show that: (i) the dynamics of acidity drop during maturation are similar from one plot to another but staggered in time; (ii) fruit coloring occurs at different times during acidity drop; (iii) the synchronization between the coloring process and acidity drop determines both the quality of harvested fruit and the period during which orchards are harvestable, which we called the “harvestability window.” This study sheds new light on the quality of citrus harvested without fruit degreening and leads to propose actions to anticipate internal maturity evolution according to the coloring and spreading of the harvest period. The fruit acidity model obtained in this study will be extended to a practical application tool to predict fruit acidity and quality for a better-controlled harvest management
Active Food Packaging Based on Biopolymers and Aroma Compounds: How to Design and Control the Release
No full textAroma compounds are known to be efficient active agents for a broad range of applications (antimicrobial, anti-oxidant, insect repellent…) that are highly sought when aiming at extending shelf life of food or biological products. However, they are intrinsically odorant and volatile at ambient temperature, which restricts the processing routes used to introduce them in a polymeric matrix and can affect their mode of action and limit efficiency. Indeed, due to their high sensitivity toward temperature they can be lost or transformed during processing. Acting after being released in the headspace, their concentration has to be controlled to avoid any odorant contamination of the targeted products. Hence, the ability for an aroma compound to be retained in a polymeric matrix, and then released when submitted to a triggering effect, are the two main requirements that should be satisfied. The volatile nature of the aroma compound offer the possibility when introduce in the packaging to act by direct or indirect contact with the product and thus to be used in different ways; as a coating layer directly applied on the product surface, as a self-supported film or as coated paper when associated with a paper sheet, as well as an object that could be inserted in the package. As biopolymers such as proteins and polysaccharides are able to retain aroma compounds but also to favor their release by modification of their structure when the relative humidity (RH) and temperature change, they are relevant carriers of these specific aroma compounds. Examples of how active packaging systems with limonene, eugenol and carvacrol as active agents were designed and elaborated. These examples will be presented with a special focus on the processing conditions and the way to improve their aroma compound retention and the release control (biopolymer nature, cyclodextrin clay addition…). Avrami's equation has been used to model the transfer of aroma compound and to advantageously compare it taking into account the mechanism in relation to the biopolymer structural changes