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Somatic cell nuclear transfer in non-enucleated goldfish oocytes: understanding DNA fate during oocyte activation and first cellular division
No full textNuclear transfer consists in injecting a somatic nucleus carrying valuable genetic information into
a recipient oocyte to sire a diploid ofspring which bears the genome of interest. It requires that the
oocyte (maternal) DNA is removed. In fsh, because enucleation is difcult to achieve, non-enucleated
oocytes are often used and disappearance of the maternal DNA was reported in some clones. The
present work explores which cellular events explain spontaneous erasure of maternal DNA, as
mastering this phenomenon would circumvent the painstaking procedure of fsh oocyte enucleation.
The fate of the somatic and maternal DNA during oocyte activation and frst cell cycle was studied using
DNA labeling and immunofuorescence in goldfsh clones. Maternal DNA was always found as an intact
metaphase within the oocyte, and polar body extrusion was minimally afected after oocyte activation.
During the frst cell cycle, only 40% of the clones displayed symmetric cleavage, and these symmetric
clones contributed to 80% of those surviving at hatching. Maternal DNA was often fragmented and
located under the cleavage furrow. The somatic DNA was organized either into a normal mitotic spindle
or abnormal multinuclear spindle. Scenarios matching the DNA behavior and the embryo fate are
proposed
Identification of the Toxoplasma gondii mitochondrial ribosome, and characterisation of a protein essential for mitochondrial translation
No full textApicomplexan parasites cause diseases such as malaria and toxoplasmosis. The apicomplexan mitochondrion shows striking differences from common model organisms, including in fundamental processes such as mitochondrial translation. Despite evidence that mitochondrial translation is essential for parasites survival, it is largely understudied. Progress has been restricted by the absence of functional assays to detect apicomplexan mitochondrial translation, a lack of knowledge of proteins involved in the process and the inability to identify and detect mitoribosomes. We report the localisation of 12 new mitochondrial proteins, including 6 putative mitoribosomal proteins. We demonstrate the integration of 3 mitoribosomal proteins in macromolecular complexes, and provide evidence suggesting these are apicomplexan mitoribosomal subunits, detected here for the first time. Finally, a new analytical pipeline detected defects in mitochondrial translation upon depletion of the small subunit protein 35 (TgmS35), while other mitochondrial functions remain unaffected. Our work lays a foundation for the study of apicomplexan mitochondrial translation
Puberty is a critical window for the impact of diet on mammary gland development in the rabbit
No full textBACKGROUND:
Nutritional changes can affect future lactation efficiency. In a rabbit model, an obesogenic diet initiated before puberty and pursued throughout pregnancy enhances mammary differentiation, but when started during the neonatal period can cause abnormal mammary development in early pregnancy. The aim of this study was to investigate the impact of an unbalanced diet administered during the pubertal period only.
RESULTS:
Consuming an obesogenic diet at puberty did not affect either metabolic parameters or certain maternal reproductive parameters at the onset of adulthood. In contrast, at Day 8 of pregnancy, epithelial tissue showed a lower proliferation rate in obesogenic-diet fed rabbits than in control-diet fed rabbits. Wap and Cx26 genes, mammary epithelial cell differentiation markers, were upregulated although Wap protein level remained unchanged. However, the expression of genes involved in lipid metabolism and in alveolar formation was not modified.
CONCLUSION:
Taken together, our results demonstrate that the consumption for 5 weeks of an obesogenic diet during the pubertal period initiates mammary structure modifications and affects mammary epithelial cell proliferation and differentiation. Our findings highlight the potentially important role played by unbalanced nutrition during critical early-life windows in terms of regulating mammary epithelial cell differentiation and subsequent function in adulthood
Implication de la fraction minérale dans l’encrassement des évaporateurs à flot tombant
No full textLes poudres laitières sont obtenues par concentration du produit liquide (membranes, évaporation sous vide) puis séchage du concentré. Contrairement au séchage par atomisation, peu de travaux ont été menés sur la concentration par évaporation sous vide. Pourtant, la maîtrise du schéma global de production des poudres
gagnerait en une meilleure compréhension de cette opération réalisée dans des évaporateurs à flot tombant. Le présent travail vise à étudier comment améliorer les performances des évaporateurs ; il s’appuie
sur deux facteurs limitants de l’opération : l’encrassement des surfaces d’échange et la viscosité maximale en sortie des évaporateurs. La contribution des différents constituants des produits à l’encrassement a
été étudiée grâce à une méthodologie développée sur un évaporateur pilote. Les résultats obtenus ont permis d’identifier les éléments minéraux qui contribuent à l’encrassement lors de la concentration de microfiltrat et d’ultrafiltrat de lait, de lactosérums acides chlorhydrique et lactique. Afin de dépasser l’extrait sec
maximal en sortie des évaporateurs à flot tombant, deux évaporateurs rotatifs à couche mince, de conceptions différentes, ont été testés à l’échelle pilote. Les études de faisabilité ont été menées jusqu’à la poudre. Le potentiel des équipements a été évalué suivant les propriétés fonctionnelles des poudres et le coût énergétique d’élimination de l’eau. Dans l’un des cas, le remplacement de la tour de séchage par deux évaporateurs rotatifs en série constitue une rupture technologique pour la production de poudres de perméat et diminue significativement les coûts énergétiques associés
A millennium-long reconstruction of damaging hydrological events across Italy
No full textDamaging hydrological events are extreme phenomena with potentially severe impacts on human societies. Here, we present the hitherto longest reconstruction of damaging hydrological events for Italy, and for the whole Mediterranean region, revealing 674 such events over the period 800-2017. For any given year, we established a severity index based on information in historical documentary records, facilitating the transformation of the data into a continuous time-series. Episodes of hydrological extremes disrupted ecosystems during the more severe events by changing landforms. The frequency and severity of damaging hydrological events across Italy were likely influenced by the mode of the Atlantic Multidecadal Variability (AMV), with relatively few events during the warm Medieval Climate Anomaly dominated by a positive phase of the AMV. More frequent and heavier storms prevailed during the cold Little Ice Age, dominated by a more negative phase of the AMV. Since the mid-19th century, a decreasing occurrence of exceptional hydrological events is evident, especially during the most recent decades, but this decrease is not yet unprecedented in the context of the past twelve centuries
Osteogenic effect of fisetin doping in bioactive glass / polycaprolactone hybrid with dual cortical / trabecular structure.
No full textDemonstration of facilitation between microalgae to face environmental stress
No full textPositive interactions such as facilitation play an important role during the biological colonization and species succession in harsh or changing environments. However, the direct evidence of such ecological interaction in microbial communities remains rare. Using common freshwater microalgae isolated from a High Rate Algal Pond HRAP treating wastewaters, we investigated with both experimental and modeling approaches the direct facilitation between two algal strains during the colonization phase. Our results demonstrate that the first colonization by microalgae under a severe chemical condition arose from the rapid growth of pioneer species such as Chlorella sorokiniana, which facilitated the subsequent colonization of low growth specialists such as Scenedesmus pectinatus. The pioneer species rapidly depleted the total available ammonia nitrogen favoring the specialist species initially inhibited by free ammonia toxicity. This latter species ultimately dominated the algal community through competitive exclusion under low nutrient conditions. We show that microbial successions are not only regulated by climatic conditions but also by interactions between species based on the ability to modify their growth conditions. We suggest that facilitation within the aquatic microbial communities is a widespread ecological interaction under a vast range of environmental stress
Validation of Copernicus Sentinel-2 Cloud Masks Obtained from MAJA, Sen2Cor, and FMask Processors Using Reference Cloud Masks Generated with a Supervised Active Learning Procedure
No full textThe Sentinel-2 satellite mission, developed by the European Space Agency (ESA) for the Copernicus program of the European Union, provides repetitive multi-spectral observations of all Earth land surfaces at a high resolution. The Level 2A product is a basic product requested by many Sentinel-2 users: it provides surface reflectance after atmospheric correction, with a cloud and cloud shadow mask. The cloud/shadow mask is a key element to enable an automatic processing of Sentinel-2 data, and therefore, its performances must be accurately validated. To validate the Sentinel-2 operational Level 2A cloud mask, a software program named Active Learning Cloud Detection (ALCD) was developed, to produce reference cloud masks. Active learning methods allow reducing the number of necessary training samples by iteratively selecting them where the confidence of the classifier is low in the previous iterations. The ALCD method was designed to minimize human operator time thanks to a manually-supervised active learning method. The trained classifier uses a combination of spectral and multi-temporal information as input features and produces fully-classified images. The ALCD method was validated using visual criteria, consistency checks, and compared to another manually-generated cloud masks, with an overall accuracy above 98%. ALCD was used to create 32 reference cloud masks, on 10 different sites, with different seasons and cloud cover types. These masks were used to validate the cloud and shadow masks produced by three Sentinel-2 Level 2A processors: MAJA, used by the French Space Agency (CNES) to deliver Level 2A products, Sen2Cor, used by the European Space Agency (ESA), and FMask, used by the United States Geological Survey (USGS). The results show that MAJA and FMask perform similarly, with an overall accuracy around 90% (91% for MAJA, 90% for FMask), while Sen2Cor's overall accuracy is 84%. The reference cloud masks, as well as the ALCD software used to generate them are made available to the Sentinel-2 user community