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The effect of single and combined use of base isolator and fluid viscous damper on seismic performance in a conventional RC building with torsional irregularity
No full textOzer, Esra/0000-0002-7778-0119This study investigates the effects of fluid viscous damper (FVD), lead rubber bearing (LRB) and friction pendulum slider (FPS) used both separately and in combination on seismic performance of a mid-rise reinforced concrete building with torsional irregularity. Three earthquake levels were used corresponding to frequent, design and rare earthquake ground motions. 528 different bi-directional nonlinear history analyses of 3-D 5-story building models were performed. Each earthquake level was represented using 11 spectrum-compatible ground motion record pairs. Nonlinear behavior of structural members, base isolators and fluid viscous dampers was considered in the models with torsional irregularity. The roof drift ratios, inter story drift ratios, absolute acceleration profiles and torsion irregularity coefficient values and plastic damage states were compared for seismic performance evaluation. When passive systems were added to the model, seismic performances improved as expected and torsional irregularity was largely eliminated compared to the fixed-base model. Although using only FVD improved seismic performance less than using only base isolators, it should be noted that FVDs used in different numbers and configurations may change the results. The findings underscore the benefits of combined damping systems, particularly FPS and FVD in mitigating torsional effects and enhancing overall structural performance. Compared to the fixed-base model, RDR, IDR and maximum acceleration demands in this model decreased at least by 91.2 %, 74.7 % and 80.3 %, respectively. Besides, it was concluded that using a limited number of ground motion records may lead to misleading results
Piaget’in bilişsel gelişim kuramına dayalı beslenme eğitiminin okul öncesi çocuklarda besin tüketimi, beslenme ve yeme davranışına etkisi: mix metod çalışma
No full textBu araştırmanın amacı; okul öncesi çocuklara Piaget’in bilişsel gelişim kuramı kullanılarak verilen beslenme eğitiminin çocukların besin tüketimi, beslenme ve yeme davranışı üzerine etkisini incelemektir. Araştırma nitel ve nicel araştırma yöntemlerinin kullanıldığı mix metod bir çalışmadır. Araştırmanın birinci aşamasında ön- test ve son-teste dayalı yarı deneysel araştırma tasarımı kullanılmıştır. Araştırmanın örneklemini Uşak merkez İl Milli Eğitim Müdürlüğü’ne bağlı ana okullarda bulunan deney (n=127) ve kontrol (n=111) olmak üzere 238 okul öncesi çocuk ve annesi oluşturmuştur. Deney grubundakilere 6 oturumluk beslenme eğitimi verilmiştir. Veriler Sosyodemografik Bilgi Formu, Besin Tüketim Sıklığı Anketi (BTSA), Beslenme Davranış Ölçeği (BDÖ) ve Çocuklarda Yeme Davranışı Anketi (ÇYDA) kullanılarak toplanmıştır. Birinci aşama verilerinin analizinde Mann-Whitney U test, Wilcoxon test yöntemi ve Pearson-χ2 çapraz tabloları ve normal dağılıma sahip olmayan iki nicel değişkenin ilişkilerinin incelenmesinde “Spearman” korelasyon katsayısı kullanılmıştır. Araştırmanın ikinci aşamasında nitel (kalitatif) araştırma yöntemlerinden odak grup görüşme yöntemi kullanılmıştır ve örneklemini Piaget’nin bilişsel gelişim kuramı kullanılarak verilen beslenme eğitimi alan çocukların anneleri (n=18) oluşturmuştur. Annelerin verileri yarı yapılandırılmış görüşme formu kullanılarak toplanmış, niteliksel veriler içerik analizi yöntemi ile değerlendirilmiştir. Yarı deneysel araştırmada; beslenme eğitimi sonrası deney grubunun kontrol grubuna kıyasla süt, meyve, sebze, kurubaklagil, tahıl ve et grubu tüketim oranlarında olumlu değişiklikler gözlenmiştir. Müdahale sonrası kontrol grubundakilerin ön test – son test süreçlere göre BDÖ puanları açısından istatistiksel olarak anlamlı fark görülmezken deney grubundakilerin son test BDÖ puanlarının, ön teste göre anlamlı düzeyde daha yüksek olduğu belirlenmiştir. Müdahale sonrasında gruplara göre ön test ve son test ÇYDA gıda hevesli, duygusal aşırı yeme, gıdadan keyif alma, içme tutkusu, tokluk heveslisi ve yemek seçiciliği puanları açısından istatistiksel olarak anlamlı fark olmadığı tespit edilmiştir. Araştırmanın ikinci aşaması olan niteliksel araştırmada eğitim sonrası annelerle yapılan kalitatif görüşmeler sonrası ‘’eğitimin çocuğun besin tüketimine etkisi’’, ‘’eğitimin çocuğun beslenme davranışına etkisi’’ ve ‘’eğitimin çocuğun yeme davranışına etkisi’’ olmak üzere üç tema belirlenmiştir. Çalışma sonucunda okul öncesi çocuklara Piaget’nin bilişsel gelişim kuramı kullanılarak verilen beslenme eğitiminin çocukların besin tüketimini ve beslenme davranışını olumlu yönde etkilediği ancak yeme davranışı üzerinde herhangi değişiklik oluşturmadığı görülmüştürThe aim of this study was to investigate the effect of nutrition education given to preschool children using Piaget's cognitive development theory on food consumption, nutritionbehavior, and the eating behavior of children. The research is a mixed-method study in which qualitative and quantitative research methods are used. In the first phase of the study, a quasi-experimental research design based on pre-test and post-test was used. The sample of the study consisted of 238 preschool children and their mothers in the experimental (n=127) and control (n=111) groups in kindergartens affiliated with Uşak Provincial Directorate of National Education. The experimental group received six sessions of nutrition education. Data were collected using the Sociodemographic Information Form, Food Consumption Frequency Questionnaire (FFCQ), Nutrition Behaviour Scale (NBS), and Children's Eating Behaviour Questionnaire (CEBQ). Mann-Whitney U test, Wilcoxon test method, and Pearson-χ2 cross-tabulations were used to analyze the first stage data, and ‘Spearman correlation coefficient was used to examine the relationship between two quantitative variables that did not have a normal distribution. In the second stage of the study, the focus group interview method, one of the qualitative research methods, was used, and the sample consisted of mothers (n=18) of children who received nutrition education using Piaget's cognitive development theory. The data of the mothers were collected using a semi-structured interview form, and the qualitative data were evaluated by content analysis method. In the quasi-experimental study, positive changes were observed in the consumption rates of milk, fruit, vegetables, legumes, cereals, and meat groups in the experimental group compared to the control group after nutrition education. After the intervention, while there was no statistically significant difference between the pre-test and post-test NBS scores of the control group, the post-test NBS scores of the experimental group were significantly higher than the pre-test. After the intervention, it was determined that there was no statistically significant difference between the groups in terms of pre-test and post-test CBEQ scores of food responsiveness, emotional overeating, enjoyment of food1, desire to drink, satiety responsiveness, and fussiness. In the qualitative research, which is the second stage of the study, three themes were determined ‘the effect of education on a child's food consumption’, ‘the effect of education on a child's feeding behavior’ and ‘the effect of education on child's eating behavior’ after qualitative interviews with mothers after the training. As a result of the study, it was seen that nutrition education given to preschool children using Piaget's cognitive development theory positively affected children's food consumption and nutrition behavior but did not change eating behavior
Minör cerrahi işlem planlanan çocukların otonomisine yönelik ebeveyn öz yeterliliğinin preoperatif anksiyeteye etkisinin incelenmesi
No full textCerrahi işlem planlanan çocukların yaşadığı preoperatif anksiyete, çocukların hastaneye yatış sürecinde oryantasyonlarını ve anestezi indüksiyonuna uyumunu olumsuz etkilemektedir. Ebeveynlerin bu süreçte çocukların ihtiyaçlarına yönelik sağladığı öz yeterlilik davranışları çocukların preoperatif anksiyete ile baş etmesini kolaylaştırabilmektedir. Bu araştırmanın amacı, minör cerrahi işlem planlanan çocukların otonomisine yönelik, ebeveyn öz yeterliliğinin preoperatif anksiyeteye etkisinin incelenmesidir. Kesitsel ve ilişki arayıcı tipte planlanan araştırmaya, dahil edilme kriterlerini karşılayan 3-6 yaş arası 100 çocuk ve ebeveyni alınmıştır. Araştırmanın evrenini Bursa İl Sağlık Müdürlüğü bünyesine bağlı Bursa Şehir Hastanesi Çocuk Cerrahi Kliniği’ne yatışı yapılan çocuk ve ebeveynleri oluşturmuştur. Verilerin toplanmasında ‘‘Tanımlayıcı Bilgi Formu’’, ‘‘Yale Modifiye Preoperatif Anksiyete Ölçeği Çocuk Formu (YMPAÖÇF)’’, ‘‘Minör Cerrahide Çocuk Otonomisine Yönelik Ebeveyn Öz Yeterlik Ölçeği (MCÇOYEÖS)’’ kullanılmıştır. İstatistiksel analizler SPSS (IBM SPSS Statistics 27) adlı paket program kullanılarak yapılmıştır. Araştırmanın yürütülebilmesi için Pamukkale Üniversitesi Girişimsel Olmayan Klinik Araştırmalar Tıbbi Etik Kurulu’ndan ve Bursa Valiliği İl Sağlık Müdürlüğü Şehir Hastanesi Başhekimliği’nden kurum izni alınmıştır. Çalışma sonucunda ebeveyn öz yeterliliğinin preoperatif anksiyeteye etkisinin olduğu (p0,05), ebeveyn öz yeterliliği arttıkça çocukların kendisine yapılacak tıbbi işlemler konusunda bilgisinin arttığı (p0,001), hastaneye yatış öyküsünün preoperatif anksiyeteyi etkilediği (p=0,024), çocuğun kendisine yapılacak işlemler hakkında bilgi varlığı preoperatif anksiyeteyi azalttığı (p0,001) kanıtlanmıştır.Preoperative anxiety experienced by children who are scheduled for surgery negatively affects their orientation and compliance with anesthesia induction during hospitalization. Self-efficacy behaviors provided by parents for the needs of children in this process may facilitate children to cope with preoperative anxiety. The aim of this study was to examine the effect of parental self-efficacy on preoperative anxiety regarding the autonomy of children scheduled for minor surgery. In this cross-sectional and correlational study, 100 children aged 3-6 years and their parents who met the inclusion criteria were included. The population of the study consisted of children and their parents who were hospitalized in Bursa City Hospital Pediatric Surgery Clinic under Bursa Provincial Health Directorate. ''Descriptive Information Form'', ''Yale Modified Preoperative Anxiety Scale Child Form (YMPCASC)'', ''Parental Self-Efficacy Scale for Child Autonomy in Minor Surgery (PPSASC)'' were used to collect the data. Statistical analyses were performed using the SPSS (IBM SPSS Statistics 27) package program. Institutional permission was obtained from Pamukkale University Non-Interventional Clinical Research Medical Ethics Committee and Bursa Governorship Provincial Health Directorate City Hospital Chief Physician's Office for the conduct of the study. As a result of the study, it was proved that parental self-efficacy had an effect on preoperative anxiety (p0,05), as parental self-efficacy increased, children's knowledge about the medical procedures to be performed on them increased (p0.001), hospitalization history affected preoperative anxiety (p=0,024), and the presence of information about the procedures to be performed on the child decreased preoperative anxiety (p0.001)
Player Experiences and Motivations in the Game Stages of an Mmorpg From the Perspective of Product Life Cycle Theory
No full textThis study aimed to explore the gaming motivations of the players in a specific MMORPG by suggesting an individual approach to the product life cycle theory. Five questions, focused on the product life cycle stages, were used to interview. A total of 257 meaningful statements were produced during interviews and used as research data. The content analysis was conducted with two cycle coding method; 182 in vivo codes for the first cycle and 21 pattern codes for the second cycle are revealed. A hierarchical framework was generated respectively from code, category, theme, and theory to describe product life cycle stages. 21 motivational categories formed 4 themes that have similar properties with the product life cycle stages. The results reveal the motivational experiences of gamers for each theme and suggest an individual evaluation process to the product life cycle theory on the market. The practical implications findings are also discussed. © The Author(s), under exclusive licence to Springer Nature B.V. 2024
The Effect Of Genetic Alterations Detected By The Circulating Tumor Dna-based Next-generation Sequencing Technique On Prognosis And Survival İn Metastatic Colorectal Cancer;
No full textPurpose: Studies conducted to date showed that circulating tumor DNA (ctDNA)-based next generation sequencing (NGS) panels are beneficial in the treatment strategies of patients with metastatic colorectal cancer (mCRC). In this study, we planned to determine the frequencies of various genetic alterations in patients with mCRC by ctDNA-based NGS analyses, evaluate the concordance rates by comparing these results with the results in standard polymerase chain reaction (PCR) analyses, and investigate the effect of the detected alterations on overall survival and progression-free survival. Materials and methods: The study was conducted by retrospective screening and analysis of the data on 48 patients, who were followed up with a diagnosis of mCRC and who received chemotherapy and/or biological agents. The data were analyzed using SPSS 25.0 [IBM SPSS Statistics 25 software (Armonk, NY: IBM Corp.)] package program. Results: In this study, ctDNA-based NGS analyses, compared to the quantitative PCR-based gold standard method, were found to have a sensitivity rate of 64.7%, specificity rate of 55.6% and concordance rate of 59.1% for KRAS mutation; a sensitivity rate of 100%, specificity rate of 86.7% and concordance rate of 87.1% for NRAS mutation; a sensitivity rate of 50%, specificity rate of 96.4% and concordance rate of 90.6% for BRAF mutation. In addition, concordance rates were evaluated based on the time elapsed between the time of taking the liquid biopsy and tissue biopsy samples. As a result, concordance rates for KRAS, NRAS, and BRAF mutations were found to be 60.9%, 100%, and 100% respectively, in cases where this elapsed time was less than 6 months; and were found to be 57.1%, 78.9%, and 85% respectively, in cases where this elapsed time was more than 6 months. Furthermore, the comprehensive analyzes revealed that the frequency of many molecular changes in mCRC as well as the relationship of these changes with clinicopathological features and survival times. Conclusion: Our study demonstrates the clinical benefit of ctDNA-based NGS analyzes in patients with mCRC. © 2025, Pamukkale University. All rights reserved
Effects of quercetin on adipokine profile in fructose-induced metabolic syndrome;
No full textPurpose: Metabolic syndrome (MetS) is a health condition characterized by obesity, insulin resistance, dyslipidemia and type 2 diabetes (T2DM). This study aimed to assess the effects of quercetin, a natural flavonoid on MetS induced by fructose in Sprague Dawley rats. Materials and methods: The rats, aged 8-10 weeks, were divided into 4 groups: control (C) group, metabolic syndrome (MetS) group, control+quercetin (C+Q) group, and metabolic syndrome+quercetin (MetS+Q) group. The MetS groups received a 20% fructose solution in drinking water for a duration of 10 weeks. For the last 4 weeks of the study, rats in the Q groups were administered 50 mg/kg/body weight quercetin. After 10 weeks, serum samples were tested using ELISA for Triglycerides (TG), High-Density Lipoprotein (HDL), fasting insulin, resistin, (Interleukin 6) IL6, Tumor Necrosis Factor-alpha (TNF-α), leptin, C-Reactive Protein (CRP) and, adiponectin (ADP). The body weights, Lee index and HOMA-IR scores were also measured. Results: Fructose-fed rats showed significant increases in body weight, Lee index, HOMA-IR scores and, fasting insulin with significant decrease in HDL compared to controls. In MetS group, ADP levels were significantly lower compared to control group. In MetS+Q group, there was a tendency for reduced levels of resistin, IL-6, and leptin compared to the untreated MetS group. Conclusion: These findings suggest that quercetin may be beneficial in managing MetS, though further research is needed to explore its mechanisms and effectiveness. © 2025, Pamukkale University. All rights reserved
Normal Faulting and Its Role in the Drainage Divide Migration in the Karıncalıdağ Region, Menderes Massif, Western Türkiye
No full textDrainage divide migration refers to the shifting boundaries between adjacent drainage basins over time, driven by processes such as tectonic uplift, differential erosion, stream capture, and lithological variations. This phenomenon has a significant impact on water flow patterns and basin extents, serving as an indicator of the landscape's response to active tectonic forces. One of the key drivers of divide migration is asymmetric uplift, which causes divides to shift from areas of lower uplift to regions experiencing higher uplift. Drainage divides are inherently dynamic, evolving over time as drainage networks develop and adjust to changing conditions. This study focuses on the migration of the main drainage divide along Kar ; imath;ncal ; imath;da ; gbreve;, located between Bozdo ; gbreve;an and Karacasu. It employs geomorphic analyses using metrics such as the normalized steepness index (ksn), Chi (chi), and Gilbert metrics. The main divide is categorized into four segments (D1-D4), with the Karacasu Fault, situated along the mountain's north-eastern boundary, identified as the primary factor influencing divide dynamics. Secondary factors include the relatively low elevation of Kar ; imath;ncal ; imath;da ; gbreve;, uniform lithology, and consistent rainfall patterns across the region. The results indicate that the main divide is currently stable, suggesting a balance between uplift and erosion. However, higher chi values in the D4 segment suggest that future erosion may dominate, potentially causing the divide to migrate toward the Bozdo ; gbreve;an Basin. These findings highlight the dynamic nature of drainage divides and the complex interplay of tectonic, erosional, and lithological processes that shape their evolution. Continued monitoring and advanced geomorphic analysis are essential for understanding the long-term stability of the divide and its response to future tectonic activity and erosional modifications
Human Anti-Apoptotic Bcl-2 and Bcl-Xl Proteins Protect Yeast Cells From Aging Induced Oxidative Stress
No full textAging is a degenerative, biological, and time-dependent process that affects all organisms. Yeast aging is a physiological phenomenon characterized by the progressive transformation of yeast cells, resulting in modifications to their viability and vitality. Aging in yeast cells is comparable to that in higher organisms in some respects; however, due to their straightforward and well-characterized genetic makeup, these cells present unique advantages when it comes to researching the aging process. Here, we assessed the impact of human anti-apoptotic Bcl-2 and Bcl-xL proteins on aging using a yeast model. The findings clearly showed that these proteins exhibited remarkable anti-aging properties in yeast cells. Our data indicate that the presence of both proteins enhanced the reproductive survival of aging cells, likely by effecting the components functioning as both pro- and anti-oxidants, depending on the stage of yeast cell lifespan. Both proteins partially protected yeast cells from aging-related morphological deformations and cellular damage during the aging period. In particular, Bcl-xL expressing yeast cells reached the maximum activity levels for almost all of the major antioxidant enzymes and the total antioxidant status on the 8th day of lifespan and could provide effective protection at the latest stage of the investigated aging period. The chemometric data analysis of IR spectra confirmed the findings of the morphological and biochemical analyses. In this regard, specifically, understanding the mechanism of action on the cellular redox state of Bcl-xL in yeast may facilitate comprehension of its indirect antioxidant function in higher eukaryotes. (c) 2024 Elsevier B.V. and Soci ; eacute;t ; eacute; Fran ; ccedil;aise de Biochimie et Biologie Mol ; eacute;culaire (SFBBM). All rights are reserved, including those for text and data mining, AI training, and similar technologies.Scientific Research Projects Unit of Pamukkale University [PAUBAP2022FEBE040]BOLD>Funding/BOLD> This study was supported by the Scientific Research Projects Unit of Pamukkale University (PAUBAP2022FEBE040)
Yenidoğan ratlarda hiperoksiyle indüklenmiş akciğer hasarında subkutan anne sütü eksozomunun etkileri
No full textBu çalışma Pamukkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi’nin 05.06.2024 tarih ve 2024TIPF011 nolu kararı ile desteklenmiştir.Prematüre bebekleri etkileyen en önemli morbidite ve mortalite nedenlerinden birisi olan bronkopulmoner displazi (BPD) için, etkinliği kanıtlanmış kesin bir tedavi veya önleyici strateji henüz mevcut değildir. Biz bu çalışmada subkutan anne sütü eksozomunun, hiperoksik akciğer hasarı (HAH) yöntemiyle BPD modeli oluşturduğumuz yenidoğan ratların akciğerlerinde meydana getirdiği histopatolojik ve biyokimyasal etkilerini araştırmayı planladık. HAH oluşturulacak Sprague-Dawley rat yavruları postnatal birinci günden yedinci güne kadar, pleksiglastan imal edilen kapalı bir alanda hiperoksiye (oksijen konsantrasyonu %85) maruz bırakıldı. Oksijen konsantrasyonu günde iki defa kontrol edildi (anestezik gaz monitörü, Drager, 1996) ve nem oranı %60-80 arasında tutulmaya çalışıldı. Oluşan karbondioksitin soda-lime ile ortamdan uzaklaştırılması sağlandı.
Rat yavruları her birinde sekiz adet olmak üzere altı gruba ayrıldı. Grup I (oda havası, kontrol grubu, n=8), Grup II (HAH modeli, n=8), Grup III (profilaksi plasebo grubu, HAH+1.-6. günler subkutan salin uygulandı, n=8), Grup IV (profilaksi tedavi grubu, HAH+1.-6. günler subkutan anne sütü eksozomu uygulandı, n=8), Grup V (plasebo tedavi grubu, HAH+7.-11. günler subkutan salin uygulandı, n=8), Grup VI (tedavi grubu, HAH+7.-11. günler anne sütü eksozomu uygulandı, n=8). Grup I, II, III ve grup IV postnatal 7. günde, grup V ve VI ise 11. günde ötenazi edildi. Ötenazi sonrası ratların sağ akciğer doku örneklerinde alveolar yüzey alanı skoru, fibrozis ve düz kas aktin skoru değerlendirmesi yapıldı. Sol akciğer doku örnekleri total antioksidan kapasite (TAS) ve total oksidan kapasite (TOS) düzeyleri için ticari ELISA kitleri ile ve kardiyak girişimle alınan kanda ticari ELISA kitleri ile TAS ve TOS düzeyleri antijen antikor reaksiyonuna dayalı sandviç immün ölçüm yöntemi ile Tıbbi Biyokimya Araştırma Laboratuvarında analize edildi. HAH modelinde hem akciğer alveolar yüzey alanı skoru hem de düz kas aktin skoru kontrol grubuna göre belirgin yüksek saptanmış (p0,05), ancak fibrozis bakımından farklılık gösterilememiştir (p>0,05). TOS düzeyleri hem akciğer dokusu
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hem de kanda HAH grubunda kontrol grubuna göre yine yüksek ancak istatistiksel olarak anlamlı bulunmamıştır. Anne sütü eksozomunun profilaktik uygulanması hem akciğer alveolar yüzey alanı skorunu hem de düz kas aktin skorunu Grup II, III, V ve VI’ya göre belirgin olarak düşürmüş (p0,05) ve kontrol grubuyla aralarında istatistiksel bir farklılık saptanmamıştır. Ayrıca profilaktik anne sütü eksozom uygulamasının akciğer dokusu TOS düzeylerini de hem HAH grubu hem de plasebo grubuna göre belirgin düşürdüğü görülmüştür (p0,05). Sonuç olarak, hiperoksik akciğer hasarı ile BPD modeli oluşturulan bu deneysel çalışmada profilaktik anne sütü eksozomu uygulamasının hem histopatolojik hem de biyokimyasal olarak akciğer hasarını önleyici etkisinin olduğu gösterilmiştir. Son yıllarda özellikle erken tanı ve önleyici stratejiler üzerinde durulmaktadır, bu yönüyle önleyici bir tedavi yöntemi olarak anne sütü eksozumunun BPD gelişme ihtimali yüksek olan bebeklere profilaktik olarak uygulanması bakımından çalışmamızın önemli olduğunu, ancak bu alanda daha kapsamlı ve ileri çalışmalara ihtiyaç duyulduğunu düşünüyoruz.Bronchopulmonary dysplasia (BPD), which is one of the most important causes of morbidity and mortality affecting premature infants, has no proven treatment or preventive strategy. In this study, we planned to investigate the histopathologic and biochemical effects of subcutaneous breast milk exosome in the lungs of neonatal rats in which BPD was induced by hyperoxic lung injury (HLI). Sprague-Dawley rat pups in which HLI was induced were exposed to hyperoxia (oxygen concentration 85%) in a closed space made of plexiglass from postnatal day one to postnatal day seven. Oxygen concentration was checked twice a day (anesthetic gas monitor, Drager, 1996) and humidity was kept between 60-80%. Carbon dioxide was removed from the environment with soda-lime.
Rat pups were divided into six groups of eight in each. Group I (room air, control group, n=8), Group II (HLI model, n=8), Group III (prophylaxis placebo group, HLI+1.-6. days subcutaneous saline administered, n=8), Group IV (prophylaxis treatment group, HLI+1.-6. days, subcutaneous breast milk exosome administered n=8), Group V (placebo treatment group, HLI+7.-11. days, subcutaneous saline administered, n=8), Group VI (treatment group, HLI+7.-11. days, subcutaneous breast milk exosome administered, n=8). Groups I, II, III and IV were euthanized on postnatal day 7, and groups V and VI on postnatal day 11. After euthanasia, alveolar surface area score, fibrosis and smooth muscle actin score were evaluated in the right lung tissue samples of the rats. Left lung tissue samples were analyzed for total antioxidant capacity (TAC) and total oxidant capacity (TOC) levels with commercial ELISA kits, and TAC and TOC levels in blood obtained by cardiac intervention with commercial ELISA kits were analyzed by antigen antibody reaction based sandwich immunoassay method in the Medical Biochemistry Research Laboratory.
Both lung alveolar surface area score and smooth muscle actin score were significantly higher in the HLI model compared to the control group (p0.05), but no difference was found in terms of fibrosis (p>0.05). TOC levels in both lung tissue and
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blood were found to be higher in the HLI group compared to the control group, but not statistically significant. Prophylactic administration of breast milk exosome significantly decreased both lung alveolar surface area score and smooth muscle actin score compared to groups II, III, V and VI (p0.05) and no statistical difference was found between the prophylactic treatment group and the control group. In addition, prophylactic breast milk exosome administration significantly decreased lung tissue TOS levels compared to both HLI group and placebo group (p0.05).
In conclusion, in this experimental study in which hyperoxic lung injury and BPD model were established, it was shown that prophylactic breast milk exosome administration has a preventive effect on lung injury both histopathologically and biochemically. In recent years, especially early diagnosis and preventive strategies have been emphasized. In this respect, we think that our study is important in terms of prophylactic administration of breast milk exosome as a preventive treatment method to infants with a high probability of developing BPD, but more comprehensive and further studies are needed in this field
Coronary Artery Dissection in Drug-Coated Balloon Angioplasty: Incidence, Predictors, and Clinical Outcomes
No full textCoronary dissection is a potential occurrence after lesion preparation for percutaneous coronary intervention (PCI). Unlike stents, drug-coated balloons (DCBs) do not allow to cover dissections, thus demanding an assessment of their safety in this setting. This study aimed to evaluate the incidence, predictors, and clinical outcomes of dissections occurring with DCB-based PCI for de novo coronary artery disease. Consecutive patients with de novo coronary artery disease who underwent PCI with intention-to-treat DCB angioplasty, with or without stent implantation, were retrospectively enrolled between 2018 and 2022 at 2 Italian centers. The decision whether to leave a dissection untreated or to proceed with bail-out stenting was based on a combined angiographic evaluation of Thrombolysis In Myocardial Infarction flow, residual minimal lumen diameter, and persistent extraluminal contrast hang-up. The primary end point at 2-year follow-up was target lesion failure, a composite of cardiac death, target vessel myocardial infarction, and clinically driven target lesion revascularization. Among 522 DCB-treated lesions (466 patients), dissections were angiographically evident in 39.1% of cases, with 21.1% which underwent bail-out stenting and 78.9% left untreated. The incidence of bail-out stenting increased from type A to type E dissections (p for trend 0.001). Left anterior descending artery involvement (odds ratio 1.64, 95% confidence interval 1.12 to 2.39) was the strongest risk factors for dissection. Target lesion failure at 2 years occurred in 2.7% of lesions with untreated dissection compared with 4.2% of those with no dissection (log-rank p = 0.324). In conclusion, coronary dissections often complicate PCI with DCB angioplasty but do not correlate with increased risk of adverse events at midterm follow-up. (c) 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies