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Appraisal of biological activities of crude extracts with subfractions and phytochemical content of endemic Nepeta nuda L. subsp. lydiae from Turkey.
Antioxidant activity of crude water extract (CWE) of this plant and its five sub-fractions (namely,
hexane fraction (HF), chloroform fraction (CFF), ethyl acetate fraction (EAF), n-butyl alcohol fraction (BAF)
and rest of the fractions (RF) were measured using in vitro two antioxidant assays: DPPH and ferrous ion
chelating. Also, quantitative content of total flavonoids and phenols were carried out by colorimetric methods,
using aluminum chloride method and Folin Ciocalteu reagent respectively. CWE and five sub-fractions possessed
different antioxidant activities in antioxidant assays. EAF showed the most potent activity on DPPH
radicals and ferrous ion chelating activities with IC50 values of 10.45 and 16.76 μg /ml respectively. EAF had the
highest total phenolics (145.06 μg GAE/mg dried extract or fraction) and total flavonoids (50.16 μg QEE/mg
dried extract or fraction) contents. The essential oil of the aerial parts of this plant was analyzed by headspace gas
chromatography mass spectroscopy (HS-GC/MS) for the first time and fifty nine components were identified
representing 95.91% essential oil. 1,8-cineole (28.79%), nepetalactone (9.93%), sabinene (7.88%) and α-pinene
(5.71%) were identified as the major components. The essential oil analyzes results have given some clues on the
commercial usable, renewable resources and potentials usable of the Nepeta taxa
Bingöl İli Merkez İlçesi Yelesen ve Dikme Köyleri Meralarının Farklı Yöney ve Yükseltilerinde Yer Alan Bitki Türleri
Bu araştırma; Bingöl ili merkez ilçesi Yelesen ve Dikme köyleri meralarının farklı yöney ve yükseltilerinde yer alan bitki türlerinin tespiti ve birbirleriyle karşılaştırılması amacıyla 2012 ve 2013 yıllarında yürütülmüştür. İki yıllık araştırmanın sonucunda; toplam 29 bitki familyasının, 96 farklı cinsinde, 155 bitki taksonu tespit edilmiştir. Taksonların 15’i azalıcı, 9’u çoğalıcı ve 131 tanesinin de istilacı olduğu belirlenmiştir. Botanik kompozisyonda azalıcıların oranı %14.86, çoğalıcıların oranı %14.56 ve istilacıların oranı ise %70.59 olarak belirlenmiştir. Saptanan taksonların %11.0’ının buğdaygil, %18.1’inin baklagil ve %71.0’ının diğer familyalardan türlere ait olduğu ve sırasıyla en çok baklagiller (Fabaceae 28 adet), ballıbabagiller (Lamiaceae 20 adet), papatyagiller (Asteraceae 19 adet) ve buğdaygiller (Poaceae 17 adet) familyalarında yer alan bitkilerden olduğu saptanmıştır. Yöneyler bakımından; tür çeşitliliğinin en fazla olduğu yöneyin 90 tür ile kuzey yöneyi olduğu, bunu 83 tür ile batı, 74 tür ile doğu ve 71 tür ile güney yöneylerinin takip ettiği belirlenmiştir. Yükseltiler bakımından; üçüncü yükseltilerin (1704 m) 102 tür ile en zengin çeşitliliğine sahip olduğu, bunu 85 tür ile ikinci yükseltilerin (1876 m) takip ettiği, en düşük değeri ise 80 tür ile birinci yükseltilerin (1992 m) verdiği gözlenmiştir. Çalışmada; batı ve kuzey yöneylerinin tür varlığı açısından daha zengin olduğu, yükseltiler bakımından ise aşağılara doğru inildikçe tür zenginliğinin arttığı tespit edilmiştir
Essential oil composition of two Prangos Lindl. (Apiaceae) species from Turkey
The essential oil of the dried flowering aerial parts of Prangos pabularia Lindl. and Prangos peucedanifolia Fenzl. were analyzed by means of HS-SPME / GC-MS. As a result thirty four and thirty seven components were identified representing 91.3% and 89.5% of the oil of P. pabularia and P. peucedanifolia, respectively. The main constituents of P. pabularia were -pinene (32.4%), -3-carene (12.4%), germacrene D (8.1%), limonene (6.4%) and bicyclogermacrene (6.2%); whereas -pinene (38.1%), bicyclogermacrene (11.3%) and-3-carene (9.2%) were the major constituents of P. peucedanifolia. With this study, chemotypes of P. pabularia and P. peucedanifolia were detected -pinene and -3-carene. Studied plant samples were found to be rich in respect to essential oils. The results were discussed in consideration of natural products, renewable resources, chemotaxonomy and potential medical uses of these plants
Some Aromatic and Medicinal Plants From Bingöl (Turkey).
The use of aromatic and medicinal plants in developing countries has been widely observed. The increasing human population and demands in the late decades has led to over exploitation of land in many areas thus reducing the biodiversity of medicinal plants. Aromatic and medicinal plants possess odorous volatile substances and the characteristic aroma is due to a variety of complex chemical compounds. This study was carried out in order to contribute aromatic and medicinal plants knowledge of Eastern Anatolia Region of Turkey. This investigation included twenty medicinal or aromatic plant specimens collected and photographed from Bingöl provinces during the vegetation seasons 2016. With this study some medicinal and aromatic plants recorded and photographed; that might be useful for health-care programme, aromatic and medicinal plants knowledge, aromateraphy, phytoteraphy, economic agricultural policy development, alternative food programme, ethnobotany and development of drug sector
Some Natural Plants Possible to be Used as Ornamental Plants in Bingöl.
The native plants are the plant species that best
match the local environmental conditions and above
all, the plantation of the local plants in the large
ecological scales provide significant contributions
to the life of the natural living communities. The use
of local plants in landscaping and gardens provides
advantages in many aspects such as diversity in landscape,
conservation of gene resources, reduction of
storage costs, and prevention of erosion as well. The
most important advantage of using a native plant is
that decreasing the damage caused by the naturally
collection of the plant species used in the landscape
Essential oils and fatty acids of Stachys L. taxa, a chemotaxonomic Approach
The aerial parts and seeds of ten Stachys L. taxa were investigated for their essential oil and fatty
acid composition. Linanyl acetate, α-terpineol, germacrene D, β-myrcene, α-pinene, linalool, caryophyllene
oxide, β-caryophyllene, spathulenol were detected as the major components of the essential oils. Almost all
Stachys taxa have the same fatty acid pattern, except for minor differences. The most abundant components
were linoleic, oleic and palmitic acid. A cluster analysis was carried out for comparison and characterisation
of the seed and essential oil from the Stachys taxa
Chemical Composition and Antibacterial Activity of Cardamine uliginosa Bieb. Growing Wild in Eastern Region of Turkey.
Cardamine uliginosa Bieb. is a native plant belonging to Brassicaceae family which can used in ethnomedicine. This study was carried out to evaluate the essential oils composition of Cardamine uliginosa and its antibacterial activity. The plant sample was analyzed by GC/GC-MS system. Eventually twenty four components representing 91.2% of the total oil were identified. The obtained results proved the presence of twenty four components. The major component essential oil of this plant were identified as; limonene (32.6%), caryophyllene oxide (28.5%), -caryophyllene (8.4%), p-cymene (3.4%), α-terpinolene (3.3%), β-pinene (2.4%), α-copaene (2.1%), α-longipinene (1.3%), -cadinene (1.2%) and -ocimene (1.0%) was determined. The antibacterial activity of the essential oil was determined against sixteen bacterium isolates by measuring inhibition zones produced by the oil. The antibacterial activity of C. uliginosa essential oil was tested using the disc diffusion method wherein the essential oil has shown notably antibacterial effect with the inhibition zone in diameter from 2 mm (for Pseudomonas aeruginosa DSM 50070) to 11 mm (for Bacillus subtilis IM 622), with the exception of Salmonella enterica ATCC 1331 and Salmonella typhimurium NRRLE 4413 where the oils haven’t shown antibacterial activity. Also weak inhibitory effect were observed against Enterobacter aerogenes CCM 2531, Escherichia coli ATCC 25922, Pseudomonas aeruginosa DSM 50070 and Proteus vulgaris. Chemical composition and antibacterial activity of the tested Cardamine uliginosa essential oil, obtained from plant material from the eastern region of Turkey, display a significant phytomedical potential
Bitlis İli Hizan İlçesinde Üretilen Ballarda Antibiyotik Kalıntılarının Belirlenmesi
Honey has the nutritional value and by reason of be a healty food ingredient, is a valuable nutrient of animal origin consumed by humans. Diseases such as American foulbrood and European foulbrood sighted in honey bees cause great loss in bee colony. Antibiotics residues in the honey cause problem such as antibiotic resistance and allergic reactions in people who consume honey. In this study, the tetracyline group (oxytetracyline, chlorotetracyline, tetracyline, 4-epitetracyline, 4-epichlortetracyline, doxycycline) and the sulfanamide group (sulfadiazine, sulfathiazole, sulfadoxine, sulfameter, sulfabenzamide, sulfamethazine, sulfachlorpyridazine, sulfisoxazole, sulfamethoxazole, sulfadimethoxine) antibiotics residues were tried to be determined by LC/MS/MS method, honey samples taken from the filtered bees in the 20 different regions where the members of the association of honey producers in Hizan district were placed in Bitlis province. The maked analyses results in this study samples did not found antibiotics residues
In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker.
Muscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanization and grazing. Therefore, this study aimed to accelerate multiplication by devising a strategy for an efficient in vitro bulblet regeneration system of Muscari aucheri using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2, 0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet regeneration on explants. Maximum mean number of bulblets per explant was noted on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The regenerated bulblets were isolated from stem explants and cultured on MS medium containing 40 g/l sucrose; where they gained diameter and roote