Obihiro University of Agriculture and Veterinary Medicine
OAK Obihiro University Archives of KnowledgeNot a member yet
5082 research outputs found
Sort by
Harimaru: A new potato variety for a local specialty
Get PDFapplication/pdf‘Harimaru’ is a new potato variety bred from a cross between ‘Saikai 35’ as a female parent and ‘Pike’ as a male parent. Marker selection was performed for 1,647 seedlings to combine resistance genes to late blight (R1), Potato virus Y (Rychc), Potato virus X (Rx1), and golden cyst nematode (H1). In total, 194 selected clones were evaluated in the field, among which the best clone was officially released as ‘Harimaru’. Its yield was slightly lower than the local standard variety, ‘May Queen’. However, it produces tasty potatoes, that do not become mushy with long boiling times despite its high starch content. ‘Harimaru’ may become a local specialty potato and its multiple resistance to potato viruses may allow cultivation using homemade seed tubers from the previous season’s crop. © 2018, Japanese Society of Breeding. All rights reserved.journal articl
重度の喘鳴を呈したホルスタイン子牛にみられた喉頭膿瘍の1症例
Get PDFapplication/pdf4カ月齢のホルスタイン種子牛が著しい喘鳴を呈し、抗菌薬とステロイド投与に反応しなくなったため病性鑑定となった。喉頭の内視鏡検査では、披裂軟骨部の小角結節に顕著な腫大が観察された。また、血液検査では慢性炎症像が確認された。病理解剖により、披裂軟骨部の粘膜欠損部位と連続する喉頭粘膜下膿瘍が確認され、また組織検索ではグラム陽性球菌および短桿菌が確認された。本症例の喉頭膿瘍の原因は、飼料摂取に起因する粘膜損傷から細菌感染が生じたためと考えられた。journal articl
Serological and molecular surveys of babesia bovis and babesia bigemina among native cattle and cattle imported from thailand in hue, Vietnam
Get PDFapplication/pdfSerum and DNA from blood samples collected from Vietnamese yellow cattle (n=101) and cattle imported from Thailand (n=54) at a Vietnamese slaughter house were screened for Babesia bovis and Babesia bigemina infections by enzyme-linked immunosorbent assay (ELISA) and PCR. The positive rates determined by ELISA (B. bovis and B. bigemina) or PCR (B. bigemina) in the Vietnamese cattle were significantly higher than those found in Thai cattle. Some PCR-positive Vietnamese animals were ELISA-negative, whereas all PCR-positive Thai cattle were ELISA-positive, suggesting that the animals were infected in Thailand. Importing Babesia-infected cattle may lead to the introduction of new parasite strains, possibly compromising the development of anti-Babesia immune control strategies in Vietnam. © 2018 The Japanese Society of Veterinary Science.journal articl
Characterization of the suppressive effects of extremely-low-frequency electric fields on a stress-induced increase in the plasma glucocorticoid level in mice.
Get PDFapplication/pdfWe recently suggested that an increase in the plasma glucocorticoid (GC) level in immobilized mice is suppressed by a 50-Hz electric field (EF) in an EF strength-dependent manner. The present study aimed to assess the anti-stress effect of EFs in three scenarios: exposure to an EF of either 50 or 60 Hz, which are the standard power frequencies in most regions; varying levels of environmental brightness during EF exposure; complete or partial shielding of the mouse from the EF. We compared the GC levels and blood parameters among control, EF-alone, immobilization-alone, and co-treatment groups. There was no difference between EFs of 50 and 60 Hz in terms of the suppression of the immobilization-induced increase in GC, that is, the anti-stress effect upon EF exposure. Examination of the effects of three environmental illuminance levels, 0, 200, and 490 lux, revealed that the effect of the EF was influenced by environmental illuminance. Shielding of the mice from the EF by wrapping the animals with an electrically conductive sheet inhibited the EF effect, which showed a negative correlation with the area shielded. Hence, environmental illuminance and the body area exposed to the EF might influence the effects of an EF on stress-induced increases in plasma GC levels in mice. Because stress plays an important role in the onset and progression of various diseases, these findings may have broad implications for understanding the efficacy of EFs in health. Bioelectromagnetics. 39:516-528, 2018 © 2018 Wiley Periodicals, Inc.journal articl
The role of interferon-tau (IFNT) secreted from Day-7 embryo in uterine immune modulation in cattle
Get PDF帯広畜産大学博士（畜産衛生学）2018application/pdf博士学位論文大学院畜産学研究科畜産衛生学専攻Doctor’s Course of Animal and Food Hygienedoctoral thesi
脳梗塞を続発した脾梗塞の犬の1症例
Get PDFapplication/pdf11歳8カ月，避妊雌のミニチュア・ダックスフントが血小板減少，多発性の脾臓腫瘤の精査のために来院した．症例は，来院前日に突然の左前後肢の麻痺，左眼の視覚障害を呈した．血液凝固系検査ではD-ダイマーの上昇が認められた．腹部超音波検査では脾臓に低エコー性を呈する血流を欠く楔型の病変が複数認められた．頭部MRI検査では，右側頭葉を中心とした脳梗塞と合致する所見が得られた．以上より，脾梗塞，脳梗塞と診断し，血栓予防療法を行った．その後の経過は良好であり，第118病日に治療終了とし，その後11カ月経過した現在も再発は認められていない．本症例は，過去に膵炎に類似した臨床症状を呈しており，また血栓傾向を引き起こす他の疾患は否定的であったことから，多発性の梗塞病変は膵炎に起因する凝固亢進状態に伴うものであったと考えられた．journal articl
Milk Processing System in Barbasia of Sardinia (Italy), Mediterranean Area
Get PDFapplication/pdfThe purpose of this paper is to 1) understand the cheesemaking process practiced in Sardinia, 2) analyze how the people utilized the ecology to mature cheeses in the summer heat, and 3) discuss the history of the development of matured cheeses in Sardinia, Italy. The matured hard cheese named Casu crudu (S)/Fiore Sardo (I) has processed in Sardinia by the pasta cruda method. The transhumant pastoralists in Sardinia used to immediately sell or consume the freshly-made cheeses in the winter and spring when they resided in the lowland. It was also possible to mature cheeses in the lowland, because the temperature was low in the winter and spring. In the hot and dry summer, they migrated to the highland and made matured cheeses as they utilized the cold environment of the highland and their cool residence. They coated the cheese surfaces with olive oil to keep the cheeses from drying and succeeded in maturing the cheeses. They were able to mature cheeses and cope with the Mediterranean climate of hot and dry summer by skillfully utilizing the vertical migration from their year-round transhumance. The geography of Sardinia (comprising of lowland and highland of over 1,000 m above sea level), use of the environment (grazing in the lowland in the winter and fall and in the highland in the summer), and the transhumant lifestyle all contributed to the creation of matured hard cheeses unique to Sardinia.本研究の目的は，ヨーロッパ乳文化の熟成チーズ発達史を考察する一環として，1）サルデーニャで実践されているチーズ加工を把握し，2）暑熱環境下においてどのように生態環境を利用してチーズの熟成を実現させているかを分析することを通じて，3）サルデーニャにおける熟成チーズの発達史を考察することにある。サルデーニャでは，凝乳からホエイを抜く際に加熱しない pasta cruda 法によりカズ・クルドゥ(S)/フィオレ・サルド(I)と呼ばれる熟成ハードチーズをつくってきた。サルデーニャの移牧民はかつて，低地に滞在する冬・春期はつくりだてのチーズを主にすぐに売却したり食に供したりしていた。冬・春期は低地でも気温が低いため，チーズを熟成させることも可能であった。夏期には高地に移動して熟成チーズを加工していた。夏期の高温乾燥となる自然環境には，高地の涼しさとより涼しくできる住居を利用し，乾燥を防ぐにはオリーブオイルを表面に塗布し，チーズの熟成を実現させてきた。移牧という年間の高度差移動を巧みに利用し，冬期低温・夏期高温乾燥となる地中海性気候に対処してチーズの熟成を実現させてきた。それは，凝乳をホエイの中での保存から空気中への保存，凝乳のカッティングによる凝乳からの急速なホエイ排除，燻煙による脱水，加塩，涼しい場所を選んでの静置という加工法の発見・発達であったとも言える。サルデーニャの地形（低地から標高1000 m 以上の高地），環境利用（冬・秋期間に低地，夏期に高地を放牧），ライフスタイル（移牧）が，サルデーニャ独自の熟成ハードチーズを生み出していったといえる。journal articl
A 2-deoxyglucose-resistant mutant of saccharomyces cerevisiae shows enhanced maltose fermentative ability by the activation of mal genes
Get PDFapplication/pdfSaccharomyces cerevisiae MCD4 is a 2-deoxyglucose (2-DOG)-resistant mutant derived from the wild-type strain, AK46, wherein the 2-DOG resistance improves the maltose fermentative ability. In the MAL gene cluster, mutations were detected in MAL11 and MAL31, which encode maltose permeases, and in MAL13 and MAL33, which encode transcriptional activators. In maltose medium, the expression of MAL11 and MAL31 in MCD4 was 2.1 and 4.2 times significantly higher than that in AK46, respectively. Besides, the expression of MAL13 and MAL33 also tended to be higher than that of AK46. Although no mutations were found in MAL12 and MAL32 (which encode α-glucosidases), their expression was significantly higher (4.9 and 4.4 times, respectively) than that in AK46. Since the expression of major catabolite repression-related genes did not show significant differences between MCD4 and AK46, these results showed that the higher maltose fermentative ability of MCD4 is due to the activation of MAL genes encoding two maltose permeases and two α-glucosidases. © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).journal articl