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Thermal Tolerance Across Depth-Segregated Orbicella Corals
No full textCoral bleaching (loss of symbiotic algae) has decimated coral reefs globally due to rising seawater temperatures. However, some corals continue to thrive under conditions that are lethal to others, potentially due to unrecognized genotypes with varying physiological thresholds. This study examines the thermal tolerance of two coral species, Orbicella faveolata and Orbicella franksi, which inhabit different depths at Media Luna Reef, Puerto Rico. We investigated their survivorship following reciprocal transplant experiments across opposite depths (5 vs 20 m) and assessed their physiological responses to acute thermal stress using the Coral Bleaching Automated Stress System (CBASS). We employed low-coverage whole-genome sequencing (lcWGS) to characterize coral genomic variation and analyzed their photosymbiotic algal communities through ITS2 genotyping. Additionally, we studied their photo-physiology by examining photosynthesis versus irradiance (PE) curves and measuring protein, chlorophyll a, and Symbiodiniaceae density. Environmental assessments revealed significant differences in light conditions, but not in temperature or oxygen levels across depths, which influenced coral photo-physiology. Our results indicate that O. faveolata and O. franksi have diverged both genetically and physiologically over a narrow depth and light gradient. These differences are evident in their associations and densities of Symbiodiniaceae communities, respiration rates, and responses to acute thermal stress, with O. franksi demonstrating greater thermal tolerance. These findings provide valuable insights into the selective pressures shaping the evolutionary trajectories of these closely related taxa and their potential resilience to global warming
Cancelled - Genomic Analysis of Novel Sample Types Using Advanced Metagenomic and Microbiome Techniques
No full textDue to unforseen circumstances, Dr. Scott Tighe\u27s lecture (Genomic Analysis of Novel Sample Types Using Advanced Metagenomic and Microbiome Techniques), orginally scheduled for Friday February 21, 2025 has been cancelled.
On Feburary 21st, the Biological Sciences seminar series will now feature a lecture by Dr. Matthew He, entitled:
Mathematics of Bioinformatics: Algebraic Biology, Matrix Genetics, and Genetic Intelligence.
The ability to perform metagenomic techniques on samples collected from novel sample types demands high performance reagents and techniques not commonly used in most labs today. The Extreme Microbiome Project was established in 2015 to develop novel sampling and DNA sequencing protocols for both routine samples as well as the most difficult. Samples have been collected and sequenced from around the world including, soil, sea and fresh water, air, deep ocean, thermophilic, halophilic, acidic, and alkaline ecosystems from locations such as Greenland, Antarctica, the Red Sea, Romanian sulfur caves, and the International Space Station. Techniques including novel concentrating devices, high performance lysis enzymes, ultra-low biomass handling, biofilm disrupting, and working with high host background from eukaryotic organisms have all been applied. In all cases, DNA, RNA, and PCR amplicon sequencing are performed using a combination of short and long-read technologies including llumina, Singular G4, and Oxford Nanopore Technologies for characterizing functional and taxonomic features as well as MAG assembly of dark matter.
About the Presenter
Scott Tighe is currently technical director of the University of Vermont Genomics Facility and has expertise in all areas of genomics and microbiology including next generation sequencing (Illumina, Singular G4, and Oxford Nanopore MinION, GridION, and PromethION instrument), Sanger sequencing, PCR (PCR, RTqPCR, ddPCR), single cell analysis, flow cytometry, metagenomics, microbiome, biophotonics, and biomolecular detection of ultra low biomass nucleic acids in the environment. Scott first began working in microbiology in 1985 at Northern Arizona University and currently has over 200 manuscripts and presentations in molecular and environmental science both nationally and internationally. He is currently Chair of the ABRF Metagenomics Research Group and Extreme Microbiome Project (XMP), co-leader of the International Metagenomics and Microbiome Standards Alliance at NIST, Board member of the Genomics Standards Consortium, and developer the NASA uTitan payload deployed to the International Space Station to study DNA extractions in zero-gravity
Creating Annotated Bibliographies Using Academic Writer
Get PDFThis video will guide you through starting an annotated bibliography in Academic Writer, how to enter annotations, and how to format your annotated bibliography.https://nsuworks.nova.edu/library_learn_all/1073/thumbnail.jp
Telehealth and Telemedicine Part 1
No full texthttps://nsuworks.nova.edu/gwep_modules/1021/thumbnail.jp
Patricia Leavy: Social Fiction Icon
No full textThis article explores Patricia Leavy’s social fiction novels. The article details how and why Leavy’s work has been a source of inspiration, how the development of “social fiction” broadened the arts-based research landscape, and how this works makes possible new forms of creative research. Lasczik then reviews in detail much of Leavy’s extensive fiction catalog, her use of different genres, the messages embedded in her novels, and what those novels mean to readers
Patricia Leavy’s Spark as a Novel and Metaphor for Creative Interdisciplinary Research
No full textSpark by Patricia Leavy follows Sociology Professor Peyton Wilde as she travels to remote Iceland to participate in a mysterious scholarly seminar. Set in a moody grand manor with an enigmatic host and an eclectic cast of characters, the story blends intellectual intrigue with personal discovery and highlights the strength of Leavy’s social fiction: her characterisation, her use of motifs, and her attention to questions at the heart of scholarly work. This article reflects on the research tensions, interpersonal differences and everyday materialities that animate the story and raise valuable questions for interdisciplinary collaboration. For those interested in fiction and research, whether through creative writing or literary analysis, Spark shows how to blend social research and storytelling in vibrant, imaginative and poetic ways. It demonstrates the possibilities of fusing these realms to create a compelling and thought-provoking narrative