Nelson Mandela African Institution of Science and Technology

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    Evaluating performance of the improved hybrid passive and active mode solar dryers for processing Mangoes and Pineapples

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    A Dissertation Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Life Sciences of the Nelson Mandela African Institution of Science and TechnologyFarmers in East Africa have no access to modern processing technologies because they are costly and high-energy demanding to operate. As a result, they solely rely on the traditional open sun drying (OSD) method, which is ineffective and causes further loss of the produce. In response, a modified passive-mode hybrid solar dryer dubbed ‘Improved Solar Dryer (ISD)’ unit was developed. A conventional active mode hybrid solar dryer nicknamed a ‘Solar Photovoltaic and Electric (SPE)’ dryer was fabricated. In this study, the drying performance of the individual ISD and SPE dryers was evaluated and compared against that of the OSD method. The effect of the individual ISD and SPE dryers on the nutritional content and sensory quality of the solar-dried fruits, namely mangoes and pineapples, was also assessed. The fruits were dried using the ISD and SPE dryers, and OSD methods while following randomized complete block design experimental procedures. Results show that the drying performance of the ISD and SPE dryers was better than that of the OSD method. The SPE and ISD dryers took 10 and 18 hours to effectively dry the fruit products, respectively, as opposed to the 30 hours taken by the OSD method. The mean daily ambient temperature and relative humidity were 26.8oC and 26.7%, respectively. The mean drying air temperatures achieved by the ISD and SPE dryers were 27.7 and 40.3oC, respectively. The mean relative humidity achieved by the ISD and SPE dryers during the entire food drying process were 25.8 and 24.6%, respectively. The mean solar thermal energy recorded for the ISD dryer, SPE dryer, and OSD method were 4795, 5994, and 3595 W/m2 (Watts per square meter), respectively. The results suggest that the SPE dryer exhibited better drying performance than the ISD dryer. The results also confirm that the drying conditions attained by both the SPE and ISD dryers were superior to that of the OSD method. Results confirm an enhanced capacity and superior role of the ISD and SPE dryers in retaining both the sensory quality and nutritional content. Therefore, the ISD and SPE dryers are recommended for drying fruits

    Assessing intensification options of common bean cultivation to improve food security on smallholder farms in the northern highlands of Tanzania

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    A Thesis Submitted in Fulfilment of the Requirements for the Degree of Doctor of Philosophy in Life Sciences of the Nelson Mandela African Institution of Science and TechnologyComplementarities of common bean (Phaseolus vulgaris L.) with non-legume food crops and their significances to the agricultural systems are underexploited. Based on the description of this study, eight options were assessed for the sustainable intensification of common bean cultivation (through manipulations of intercropping and rotation) against the monocultures of maize (Zea mays L.), and the improved and local varieties of common bean in the northern highlands of Tanzania. The factors assessed were the cropping seasons/years (S) (2015 to 2017), agro-ecological zones (A) above sea level (lower 843 m, middle 1051 m, upper 1743 m), cropping systems (C) (sole, intercrop, rotation), and bean varieties (V) (improved Lyamungu 90 and local Mkanamna) and their interactions. Results indicated that S, A, C, and S×A, S×C, S×A×C were significant and bean grain yields increased in intercrops ranging from 1.5 to 2.9 t ha-1 with land equivalent ratio (LER) of 1.58. Intercropping over five cropping seasons indicated that with S×V grain yields increased from 0.2 to 3.5 t ha-1 in bean and from 2.3 to 2.6 t ha-1 in maize with LERs of 1.48 and 1.55. In rotations, higher bean grain yields were attributed to S (3.3 t ha-1 ), C (3.4 t ha-1 ), and V (2.7 t ha-1 ) and for maize were in C (2.9 t ha-1 ) and S (2.6 t ha-1 ). In conclusion, out of eight assessed options, this study found two main useful options for improving food security on smallholder farms in the northern highlands of Tanzania. The options were continuous cultivation of the improved and/or local varieties of common bean in intercrops with the maize throughout two rainy seasons of the year (long and short). Another option was cultivation of the improved and/or local varieties of common bean intercropped with maize in the long rainy season and rotating of these intercrops with the maize cultivated in the short rainy seasons. Importantly, the improved bean variety Lyamungu 90 was heavier in weight, using the same number of seeds, than the local bean variety Mkanamna, which provided additional factors to be considered to improve income where weight is the acceptable standard in the market

    Sanger sequence automatic analysis tool development

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    A Dissertation Submitted in Partial Fulfillment of the Requirements for the Degree of Master’s in Information and Communication Science and Engineering of the Nelson Mandela African Institution of Science and TechnologyThe Sanger sequencing technique still remains the cornerstone methods for the Deoxyribonucleic acid (DNA) sequencing. This is due to its high accuracy in targeting smaller genomic regions in a large number of samples, sequencing of variable regions and validating results of other DNA sequencing platforms such as those from next-generation sequencing studies. The analysis of Sanger sequence DNA data is computationally intensive requiring efficient and high computational power software tools. The most preferred tools are the proprietary licensed tools since they offer user-friendly interface and they offer more DNA analysis functionalities. However, the affordability of the tools may be limited especially for individual researchers or students due to their expensive licenses. Nevertheless, free and open source licensed tools are available and but are not user-friendly, have steep learning curve since some lack graphical user interface, operating system platform dependent and have limited functionalities. This study presents a Sanger Sequence Automatic Analysis Tool (SSAAT), a software tool designed and implemented as a web application that provides a user-friendly graphical interface such as those provided in proprietary tools. The tool has abilities to extract raw data from sequence AB1 files, make base-calls, plot chromatogram, polymorphism detection, sequence alignment and report generation. Furthermore, the tool is free and open source that can be easily accessed online through standard web browser applications. With the above-mentioned features, SSAAT can be used by molecular biologist as an alternative to proprietary tools and get comparable experience and DNA sequence analysis result

    Mobile-based business-to-business platform for the pharmaceutical industry in Tanzania

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    A Dissertation Submitted in Partial Fulfilment of the Requirements for the Degree of Masters of Science in Embedded and Mobile Systems (EMoS) of the Nelson Mandela African Institution of Science and TechnologyThe right to the uppermost attainable standard of health is a fundamental human right. In everyday life, mobile phones have become essential devices for most people in both developed and developing countries. Paper-based ordering of medicines in the pharmaceutical industry is time consuming and can enhance the spread of diseases such as COVID-19. Electronic ordering and stock management can solve these challenges, but while it has been widely adopted in developed countries, it remains underused in Tanzania. This study aimed to develop a mobile application (DawaFasta) and web application which links wholesale and retail pharmacies in Tanzania, to support electronic ordering and stock management. System and user requirements were collected through questionnaires, interviews and observations from 105 wholesale and retail pharmacies in Arusha, Dar es salaam and Kilimanjaro regions of Tanzania. The developed applications were evaluated for acceptability and usability by 9 wholesale and 15 retail pharmacy personnel to assess serviceability and usefulness. The results show that 96% found it very useful. The main reason is to bridge the gap between wholesale and retail pharmacies and provide easy access to medications. The 4% were not sure because they were unable to distinguish between legitimate and illegitimate online pharmacies. The application was assessed as having good usability for online pharmacy business purposes by wholesale and retail pharmacies. This application would need further development in order to raise awareness and add more feature

    Assessment of nutritional status and dietary aflatoxins exposure in Children in Singida District Council

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    A Dissertation Submitted in Partial Fulfillment of the requirements for the Degree of Master’s in Life Sciences of the Nelson Mandela African Institution of Science and TechnologyFeeding practices are the critical determinant of the growth and development of children during the first 1000 days of life with a long time effect on adulthood. This study aimed at assessing the diversity and safety complementary foods in terms of aflatoxins (AF). A total of 290 households with infants aged between 6-24 months were recruited from 10 villages in Singida District. Mothers were interviewed on infant feeding practices and handling of food crops using a structured questionnaire. Anthropometric measurements of index child were taken, followed by collection of 1 to 3 cereals based food samples used in complementary feeding in 180 households. A total of 218 flour samples made from maize, sorghum, millet, composite and fermented-germinated sorghum locally known as magai and magai drink; from magai flour were collected. Food samples were analyzed for aflatoxins using High-Performance Liquid Chromatography (HPLC). Anthropometric measurements showed that 37.7% were stunted; 20.3% underweight and 8.3% wasted. Furthermore, aflatoxin B1 was detected in 80 (36.7%) out of 218 complementary food samples. Aflatoxin B1 levels ranged from 0.33 to 23.75 µg/kg whereas, total aflatoxins were detected in 185 (84.9%) of 218 samples in a range of 0.47 to 289.28 µg/kg. However, mean dietary exposure to all food were; magai drink (133.36 ng/kg body weight per day), composite flour (106.0 ng/kg body weight per day), maize flour (111.70 ng/kg body weight per day), sorghum flour (94.68 ng/kg body weight per day) and millet flour (92 ng/kg body weight per day)

    Modeling the effect of binding kinetics in spatial drug distribution in the brain

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    A Dissertation Submitted in Partial Fulfilment of the Requirements for the Degree of Masters in Mathematical and Computer Sciences and Engineering of the Nelson Mandela African Institution of Science and TechnologyKey factors in the process of drug delivery in the human brain are blood-brain barrier, drug dis tribution and drug binding kinetics. Since human brain is entirely unavailable for experimenta tion, mathematical models have become vital for improved visualization of how each of the key factors affects the delivery of drugs in the the human brain. In this study, a 3-dimensional math ematical model that incorporates drug transport across the blood-brain barrier, binding kinetics and drug distribution within the brain extracellular fluid with a bidirectional bulk flow of the brain extracellular fluid was developed and simulated. The model was developed assuming a cube volume of a brain unit that is a union of the blood-brain barrier, brain extracellular fluid and the blood plasma sub-domains. The model includes a set of partial differential equations and boundary conditions that characterize the processes in the specified sub-domains. To determine the effect of drug binding kinetics, the model equations together with their prescribed boundary conditions were discretized by employing the finite difference method implicit schemes and the model was analysed and simulated using MATLAB coding. Effects of drug binding kinetics were investigated by varying the binding parameter values for both specific and non-specific binding sites. All variations of binding parameter values were discussed and the results show the improved visualization of the effect of binding kinetics in drug distribution within the brain. For more realistic visualization, it was suggested to integrate more brain components that make up the large volume of the brain tissu

    Optimized processing method for producing Avocado and Mango seed-based composite flour for functional Foods

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    A Dissertation Submitted in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in Life Sciences of the Nelson Mandela African Institution of Science and TechnologyAvocado and mango seed kernels are considered as wastes although they are rich in essential nutrients and bioactive compounds for human health. Lack of commercial application, presence of anti-nutrients, difficulty in processing and little information on nutritional and functional values contribute significantly to their underutilization. These factors underscore the need for processing these seeds into consumable products to utilize them as functional foods. The aim of this study was to optimize a processing technique capable of reducing antinutrients to acceptable levels (above 50%), retain nutrients and functional values, and improve sensory qualities of avocado and mango seeds-based composite flour for functional foods. Different processing conditions for probiotic fermentation, boiling and soaking techniques were studied to optimize processing conditions for the seeds. The antinutrients, antioxidant activity, total phenolics and selected nutrients of the seeds were analyzed using standard analytical methods. Furthermore, avocado and mango seeds-based composite flour was formulated using Nutrasheets integrated with the USDA National Nutrient Database for Standard Reference. The formulated composite flour was assessed for safety and quality parameters for a period of 84 days under different storage temperatures. The results showed that all processing techniques significantly (p < 0.05) reduced the antinutrients by over 50% for processed avocado and mango seed kernels. The highest total phenolics and antioxidant activity (IC50) for both seeds were observed at a fermentation temperature of 37oC with Lactobacillus plantarum. Soaking and boiling reduced the analyzed minerals in both avocado and mango seed kernel except for potassium and zinc on soaked mango seeds, whereas probiotic fermentation retained 100 % or increased the concentration of the analyzed minerals, ascorbic acid and α-tocopherol. In contrast, boiling and soaking reduced the contents of ascorbic acid and antioxidant activity of the analyzed samples. The Nutrasheets analysis showed that the formulated composite flour can achieve the nutritional and functional values for the functional foods. The shelf life analysis showed that 115 days (4 months) is the maximum shelf life for the flour where all the assessed safety and quality parameters can still be within the maximum recommended tolerable limits for human consumption. Therefore, the results of this study indicate that unlike boiling and soaking methods, the probiotic fermentation is an optimized processing method which can significantly reduce the antinutrients to acceptable levels, retain nutrients and bioactive compounds, and improved sensory attributes of avocado and mango seeds-based composite flour for functional foods

    Cropping patterns along an altitudinal gradient and their implications to wildlife conservation in Rombo, Tanzania

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    This research article published by Elsevier B.V., 2021Ecosystem and livelihood sustainability of small-scale farmers in Eastern Africa are often challenged by climate change and unsustainable land use practices. Little is known on the small-scale temporal processes of cropping systems in relation to long-term climatic conditions. To understand the climatic influence on the cropping patterns, and its implications to wildlife/elephant conservation, our study used a combination of time-series datasets on crop and climate as well as geo-spatial layers spanning more than three decades. To validate the information analyzed from secondary data, we carried out participatory observation and interviews, whereby about 5% of village households were selected along an altitudinal gradient of Rombo area, Tanzania. We generated land use change maps using Geographical Information System (ArcGIS 10.4) and used Spearman’s correlation analysis to assess the influence of rainfall, temperature, and crop yield on the observed longitudinal changes in the crop types and other land use patterns both within and across altitudinal gradients. We found that perennial crops like coffee and banana concentrated in upper agro-ecological zones, with a clear division line above 1230 masl while seasonal crops like maize and beans were mostly cultivated in lowlands (below 1230 masl). The results further showed that coffee yields significantly declined with higher average annual minimum temperatures over the last 16 years. Within the same time period, there was a disproportionate shift from coffee to seasonal crop cultivation such as maize and beans. Elephant numbers declined by about 38%, while human settlement area increased by 28% over the last 30 years. We conclude that changes in land use and shifts in climate and cropping patterns have important implications for elephant conservation and sustainability of the ecosystem in Rombo area, Tanzania

    Detection of Username Enumeration Attack on SSH Protocol: Machine Learning Approach

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    This research article published by MDPI, 2021Over the last two decades (2000–2020), the Internet has rapidly evolved, resulting in symmetrical and asymmetrical Internet consumption patterns and billions of users worldwide. With the immense rise of the Internet, attacks and malicious behaviors pose a huge threat to our computing environment. Brute-force attack is among the most prominent and commonly used attacks, achieved out using password-attack tools, a wordlist dictionary, and a usernames list—obtained through a so-called an enumeration attack. In this paper, we investigate username enumeration attack detection on SSH protocol by using machine-learning classifiers. We apply four asymmetrical classifiers on our generated dataset collected from a closed-environment network to build machine-learning-based models for attack detection. The use of several machine-learners offers a wider investigation spectrum of the classifiers’ ability in attack detection. Additionally, we investigate how beneficial it is to include or exclude network ports information as features-set in the process of learning. We evaluated and compared the performances of machine-learning models for both cases. The models used are k-nearest neighbor (K-NN), naïve Bayes (NB), random forest (RF) and decision tree (DT) with and without ports information. Our results show that machine-learning approaches to detect SSH username enumeration attacks were quite successful, with KNN having an accuracy of 99.93%, NB 95.70%, RF 99.92%, and DT 99.88%. Furthermore, the results improve when using ports information

    IoT-based system for automated floodwater detection and early warning in the East Africa Region: a case study of Arusha and Dar es salaam, Tanzania

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    A Dissertation Submitted in Partial Fulfilment of the Requirements for the Degree of Master of Science in Embedded and Mobile Systems of the Nelson Mandela African Institution of Science and TechnologyClimate change is a major cause of the increase of environmental natural disasters like floods, droughts, and storms. Although several countries have been affected by such natural disasters, the East African region is one of the most affected. This work focuses on floods as the most frequent disaster in the East African region where 280 died and about 2.8 million people were affected from the floods that occurred in 2019 alone. Different techniques have since been developed to mitigate the effects of floods. However, the methodologies used have not responded to the identified problems that include lack of community awareness, information inadequacy, and low-cost systems. To solve these problems, the present study aims at developing a low-cost system that detects and alerts the community on upcoming flood incidents. The proposed floodwater detection and early warning system comprise of three units. The sensing unit continuously monitors environmental parameters using ultrasonic, temperature and humidity sensors. The processing unit processes and analyses the collected data from sensors then, the alerting unit alerts the community and local authorities using a buzzer and a Short Message Service notification. The system uses the Global System for Mobile Communication to provide internet connectivity which enables data to be collected, stored, and monitored in the cloud. The system was implemented at Themi river and results showed that floods can be detected and the community near the flood-prone area alerted early. Therefore, the use of the developed system in flood-prone areas can contribute to environmental disaster risk mitigation

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