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Identification of Closely Related Polymorphisms with Striga Resistance Using Next Generation Sequencing
Molecular markers are powerful tools to enhance speed of breeding. Recent advances of sequencing technology allow us to identify most polymorphisms in a genome. Whole genome resequencing of an F2 segregating population can identify those polymorphisms that co-segregate with a mutant phenotype, which theoretically include the gene responsible for Striga resistance gained through mutagenesis. This protocol explains the isolation of high-quality genomic DNA for sequencing and step-by-step procedures for single nucleotide polymorphism (SNP) identification from bulked F2 sequences.journal articl
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奈良先端科学技術大学院大学修士(工学)master thesi
ポリサルコシン ポリニュウサン コウブンシ アシバザイ ノ ヒト iPS サイボウ ブンカ ユウドウ エノ テキヨウ ニカンスル ケントウ
奈良先端科学技術大学院大学修士(理学)master thesi
Effect of Weak Localization on Microwave Conductivity in Two-Dimensional Superconductors at Absolute Zero
The weak localization correction for the linear absorption spectrum of the two-dimensional s-wave superconductor is calculated at absolute zero. This correction term does not diverge even in a two-dimensional system, and its dependence on frequency is similar to that of Mattis–Bardeen’s conductivity. The effect of the Coulomb interaction coupled with superconducting fluctuations mainly contributes to the correction term. The excitation energy of the collective mode due to this coupling remains low in a two-dimensional system, and it is proportional to the square root of the wavenumber. The dispersion of this excitation mode is related to the absolute value of the interaction vertex, and therefore affects the magnitude of the weak localization correction terms.conference pape