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Stem cell regulators drive a G1 duration gradient during plant root development
No full textOrganogenesis relies on the coordination of cell proliferation with developmental programs. In meristems, where new plant organs initiate, the cell proliferation potential depends on stem cell regulators, but the mechanisms linking their local activity with the cell cycle machinery remain unknown. Here we show a positional gradient of G1 duration in the Arabidopsis root meristem spanning from ~2 h near the meristem boundary to more than 20 h in the early stem cell derivatives. Mutations in the stem cell regulatory PLETHORA (PLT) genes and the cell cycle RETINOBLASTOMA-RELATED 1 gene shortened G1 duration, abolishing the G1 duration gradient, whereas PLT2 overexpression increased G1 duration. Data-driven computer modelling supported the presence of an incoherent feed-forward loop. We found that PLT genes are drivers with simultaneous and opposing roles in maintaining stem cell activity and inhibiting G1 progression through a cascade involving the CDK inhibitor KRP5, a PLT target and RETINOBLASTOMA-RELATED 1. The G1 duration gradient is developmentally regulated and established after the emergence of lateral roots from the primary root and correlates with increased tolerance to genome damage. Our study establishes a previously undescribed proximal–distal G1 duration gradient during root development that is shaped by the balanced activity of stem cell maintenance and cell cycle regulators.journal articl
Optimizing Speech Translation for Low Latency and High Robustness
Full text link奈良先端科学技術大学院大学博士（工学）doctoral thesi
Physical Layer Security in mmWave Wireless Communication Systems
Full text link奈良先端科学技術大学院大学博士（工学）doctoral thesi
CDK inhibitor-mediated regulation of stemness in the Arabidopsis columella root cap
Full text link奈良先端科学技術大学院大学博士（ﾊﾞｲｵｻｲｴﾝｽ）doctoral thesi
ペプチドリンカー オ リヨウ シタ カンジョウ シトクロム c555 タリョウタイ ノ コウチク ト イオン ニ ヨル シトクロム c2 リョウタイ ノ アンテイカ
Full text link奈良先端科学技術大学院大学博士（理学）doctoral thesi
ダイキボ ゲンゴ モデル オ モチイタ ヒント セイセイ シュホウ ノ プログラミング エンシュウ ニ オケル ユウヨウセイ ヒョウカ
Full text link奈良先端科学技術大学院大学修士（工学）master thesi
Quantifying uncertainty using Gaussian processes with microbial community dissimilarities
Full text link奈良先端科学技術大学院大学博士（工学）doctoral thesi
タンイツ ガクシュウ ニ ヨル ヘンブン ジョウホウ ボトル ネック ホウ ノ トレードオフ ノ タンサク
Full text link奈良先端科学技術大学院大学博士（工学）doctoral thesi
Artificial induction of the UPR by Tet-off system-dependent expression of Hac1 and its application in Saccharomyces cerevisiae cells
No full textIn response to endoplasmic reticulum (ER) stress, yeast Saccharomyces cerevisiae cells produce Hac1, which is a transcription factor responsible for the unfolded protein response (UPR). When Hac1 is unregulatedly expressed from a constitutive promoter, the ER is artificially enforced and enlarged, even without ER stress stimuli. However, such cells are unsuitable for applicative bioproduction because they grow quite slowly and quickly lose their high-UPR phenotype upon their long-term storage. To avoid this problem, we constructed S. cerevisiae plasmids for Hac1 expression under the control of the inducible Tet-off promoter. Yeast cells carrying these plasmids did not exhibit a considerable UPR and grew rapidly when the Tet-off promoter was repressed by doxycycline. In contrast, under the Tet-off inducing condition, these plasmids caused UPR induction, growth retardation, and ER expansion, depending on the copy number of the plasmid. Moreover, as expected, lipidic molecule production was increased under these conditions.journal articl