13197 research outputs found
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Membrane remodeling activity of a mitochondrial protein
Get PDF奈良先端科学技術大学院大学博士（ﾊﾞｲｵｻｲｴﾝｽ）doctoral thesi
Interactions between Lipid II flippase and a toxic peptide that affects bacterial peptidoglycan biosynthesis
Get PDF奈良先端科学技術大学院大学博士（ﾊﾞｲｵｻｲｴﾝｽ）doctoral thesi
Behavioral and molecular analysis of fear response using zebrafish model
Get PDF奈良先端科学技術大学院大学博士（ﾊﾞｲｵｻｲｴﾝｽ）doctoral thesi
イネ ニ オケル セイチョウ ソクシン キョウセイ サイキン ノ ドウテイ ト キョウセイ セイギョ キコウ ノ カイセキ
Get PDF奈良先端科学技術大学院大学博士（ﾊﾞｲｵｻｲｴﾝｽ）doctoral thesi
Studies on construction and stabilization of hemoprotein supramolecules with helical linkers
Get PDF奈良先端科学技術大学院大学博士（理学）doctoral thesi
アデニルサン キナーゼ ノ コウゾウ ジョウタイ オ ニンシキ スル ジンコウ ケツゴウ タンパクシツ ノ ソウセイ ト フクゴウタイ ケイセイ キコウ ノ カイメイ
Get PDF奈良先端科学技術大学院大学博士（理学）doctoral thesi
ヒト サンジゲン バイヨウ カクマク ジョウヒ オ モチイタ テンガンザイ ノ キュウシュウ ソクシンザイ ト シテノ サイボウ トウカセイ ペプチド ノ タンサク ケンキュウ
Get PDF奈良先端科学技術大学院大学博士（工学）doctoral thesi
Comparison of the Metabolite Profiles of HT-29 Colorectal Cancer Cells Treated with Curcumin, Cisplatin, 5-Fluorouracil and Doxorubicin in a Metabolomic Approach
No full textChemotherapy for colorectal cancer often leads to significant adverse effects on patients, underscoring the need for alternative treatments. Herbal medicines like curcumin are considered a valuable complementary therapy due to their low toxicity profile and potential to mitigate the side effects of chemotherapy. Curcumin's mechanism of action targets multiple pathways, with untargeted metabolomic analysis helping to understand its exact mechanisms and subsequent treatment response. The aim of this study was to compare HT-29 cancer cell metabolites after curcumin and chemotherapy drug interventions to identify metabolites that can predict similar mechanisms of action between these treatments. Principal Component Analysis (PCA) of Fourier transform infrared spectroscopy (FTIR) absorption spectrum showed similar metabolite profiles in HT-29 cell culture media treated with curcumin and the chemotherapeutic cisplatin. Five cell metabolomes emerged after additional gas chromatography mass spectrometry/mass spectrometry (GC-MS/MS) and MS-DIAL data annotation: 1-bromo-2-chloroethane, 2-cyanoacetamide, dimethylamine (DMA), 2-nitrobenzo acid, and butane. The confusion matrix of these five annotated metabolites could be distinguished in HT-29 cell cultures treated with curcumin, but not in control cell cultures or those treated with the drugs cisplatin, doxorubicin, or 5-fluorouracil (5-FU). 2-cyanoacetamide in particular can be used as a marker of HT-29 cells' response to treatment with curcumin based on a p-value of < 0.05. According to these findings, no metabolite can predict the resemblance of curcumin's mechanism of action to chemotherapeutic medicines. Further study should therefore focus on in vivo experimental validation and upgrading metabolomic analysis technologies to further establish the similarities in the metabolite profiles of curcumin and cisplatin treatments.journal articl
Front-light Structure with Excitation Light Polarized for Enhancing Lensless Fluorescence Imaging
No full textIn this study, we introduce a high-performance front-light structure integrated with optical polarization control to improve low-contrast fluorescent imaging in lensless devices. Lensless fluorescence imaging is an advanced technology with the potential to enhance the performance of biological and neurological imaging. However, there are still challenges to achieving high image quality, particularly in low-contrast fluorescent signals due to light scattering and absorption. The performance of a low-fluorescence stripe diffractor, fabricated using Norland Optical Adhesive 63 (NOA 63), was evaluated in comparison to polydimethylsiloxane (PDMS) material as a light guide. The diffractor is designed to operate with a hybrid emission filter on the image sensor. As a result, by coupling a 450 nm laser with either P- or S-polarization, it was shown that NOA 63 is notable as a diffractor material. Notably, S-polarized light showed a great excitation intensity and an enhanced contrast in fluorescent bead imaging compared with P-polarized light. Furthermore, the system was validated for biological applications by successfully detecting fluorescent bead emission in agarose-embedded samples of various thicknesses, simulating brain tissue. These results emphasize the system’s ability to capture lowintensity fluorescence signals from deep layers of biological tissue.journal articl