13197 research outputs found
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トクテイ ノ セイシツ オ モツ ブンシ コウゾウ オ セイセイ スル ジコ カイキ ガタ セイセイ モデル ノ ヒカク ケンショウ
Get PDF奈良先端科学技術大学院大学修士(工学)master thesi
Phosphorylation of PACSIN2 at S313 Regulates Podocyte Architecture in Coordination with N-WASP
No full textChanges in the dynamic architecture of podocytes, the glomerular epithelial cells, lead to kidney dysfunction. Previous studies on protein kinase C and casein kinase 2 substrates in neurons 2 (PACSIN2), a known regulator of endocytosis and cytoskeletal organization, reveal a connection between PACSIN2 and kidney pathogenesis. Here, we show that the phosphorylation of PACSIN2 at serine 313 (S313) is increased in the glomeruli of rats with diabetic kidney disease. We found that phosphorylation at S313 is associated with kidney dysfunction and increased free fatty acids rather than with high glucose and diabetes alone. Phosphorylation of PACSIN2 emerged as a dynamic process that fine-tunes cell morphology and cytoskeletal arrangement, in cooperation with the regulator of the actin cytoskeleton, Neural Wiskott$2013Aldrich syndrome protein (N-WASP). PACSIN2 phosphorylation decreased N-WASP degradation while N-WASP inhibition triggered PACSIN2 phosphorylation at S313. Functionally, pS313-PACSIN2 regulated actin cytoskeleton rearrangement depending on the type of cell injury and the signaling pathways involved. Collectively, this study indicates that N-WASP induces phosphorylation of PACSIN2 at S313, which serves as a mechanism whereby cells regulate active actin-related processes. The dynamic phosphorylation of S313 is needed to regulate cytoskeletal reorganization.journal articl
Pseudo-haptics -past, present and future research trends-
No full textvideo/mp4Pseudo-haptics technique is a technique that enables the modification of haptic perception by appropriately changing multimodal (mainly visual) feedback to body movements using the interaction of sensory information in the cognitive process. Pseudo-haptics technique has attracted much attention, especially in the fields of virtual reality (VR) and user interfaces, because it can present haptic perception without bulky devices. In this presentation, we will overview the past, present and future research trends of pseudo-haptics technique including my work.講演日:2023年6月15日 3限講演場所: 情報科学棟 エーアイ大講義室(L1)vide
Abstractive Document Summarization with Summary-length Prediction
No full textRecently, we can obtain a practical abstractive document summarization model by fine-tuning a pre-trained language model (PLM). Since the pre-training for PLMs does not consider summarization-specific information such as the target summary length, there is a gap between the pre-training and fine-tuning for PLMs in summarization tasks. To fill the gap, we propose a method for enabling the model to understand the summarization-specific information by predicting the summary length in the encoder and generating a summary of the predicted length in the decoder in fine-tuning. Experimental results on the WikiHow, NYT, and CNN/DM datasets showed that our methods improve ROUGE scores from BART by generating summaries of appropriate lengths. Further, we observed about 3.0, 1,5, and 3.1 point improvements for ROUGE-1, -2, and -L, respectively, from GSum on the WikiHow dataset. Human evaluation results also showed that our methods improve the informativeness and conciseness of summaries.conference pape
ギョウ タンイ ノ イゾン カンケイ オ モチイタ テスト センタク シュホウ ノ テイアン
Get PDFテスト実行のコストを削減するための手法の１つとして，テストケース選択が活用されている．既存研究では，ファイルやメソッドレベルでプロダクトコードとテストの依存関係を取得し，テスト選択を行うことで，テスト実行のコストを大幅に削減している．しかし，メソッド単位で依存関係を取得した場合でも，分岐等によって実際に実行されない命令が存在する場合があり，不要なテストケースが含まれている可能性がある．そこで本研究では，プロダクトコードの行とテストの依存関係を利用したテストケース選択手法を提案する．journal articl
Synthesis of polyacene by using a metal$2013organic framework
No full textThe acene series, an important class of linearly polycyclic aromatic hydrocarbons, are of interest owing to their unique physicochemical features. With an increase in the number of fused benzene rings, acenes display an evolution of electronic structure and properties. Thus, efforts have been devoted to the synthesis of longer acenes, with dodecacene being the longest acene (12 fused benzene rings) reported to date. However, the formation of polymeric acenes with numerous benzene rings, namely polyacene, has yet to be realized. Herein, we present a methodology for the synthesis of polyacene mediated by a metal2013organic framework and the subsequent dehydro-aromatization reaction produced polyacene that was overwhelmingly longer than the previously reported acenes. The scalable synthesis of polyacene allowed us to unveil the stability and electronic properties of polyacene, paving the way for their widespread applications in optoelectronic and magnetic devices.journal articl
Root PRR7 Improves the Accuracy of the Shoot Circadian Clock through Nutrient Transport
No full textThe circadian clock allows plants to anticipate and adapt to periodic environmental changes. Organ- and tissue-specific properties of the circadian clock and shoot-to-root circadian signaling have been reported. While this long-distance signaling is thought to coordinate physiological functions across tissues, little is known about the feedback regulation of the root clock on the shoot clock in the hierarchical circadian network. Here, we show that the plant circadian clock conveys circadian information between shoots and roots through sucrose and K+. We also demonstrate that K+ transport from roots suppresses the variance of period length in shoots and then improves the accuracy of the shoot circadian clock. Sucrose measurements and qPCR showed that root sucrose accumulation was regulated by the circadian clock. Furthermore, root circadian clock genes, including PSEUDO-RESPONSE REGULATOR7 (PRR7), were regulated by sucrose, suggesting the involvement of sucrose from the shoot in the regulation of root clock gene expression. Therefore, we performed time-series measurements of xylem sap and micrografting experiments using prr7 mutants and showed that root PRR7 regulates K+ transport and suppresses variance of period length in the shoot. Our modeling analysis supports the idea that root-to-shoot signaling contributes to the precision of the shoot circadian clock. We performed micrografting experiments that illustrated how root PRR7 plays key roles in maintaining the accuracy of shoot circadian rhythms. We thus present a novel directional signaling pathway for circadian information from roots to shoots and propose that plants modulate physiological events in a timely manner through various timekeeping mechanisms.journal articl
Multi-Region Microdialysis Imaging Platform Revealed Dorsal Raphe Nucleus Calcium Signaling and Serotonin Dynamics during Nociceptive Pain
No full textIn this research, we combined our ultralight micro-imaging device for calcium imaging with microdialysis to simultaneously visualize neural activity in the dorsal raphe nucleus (DRN) and measure serotonin release in the central nucleus of the amygdala (CeA) and the anterior cingulate cortex (ACC). Using this platform, we observed brain activity following nociception induced by formalin injection in the mouse’s hind paw. Our device showed that DRN fluorescence intensity increased after formalin injection, and the increase was highly correlated with the elevation in serotonin release in both the CeA and ACC. The increase in calcium fluorescence intensity occurred during the acute and inflammatory phases, which suggests the biphasic response of nociceptive pain. Furthermore, we found that the increase in fluorescence intensity was positively correlated with mouse licking behavior. Lastly, we compared the laterality of pain stimulation and found that DRN fluorescence activity was higher for contralateral stimulation. Microdialysis showed that CeA serotonin concentration increased only after contralateral stimulation, while ACC serotonin release responded bilaterally. In conclusion, our study not only revealed the inter-regional serotonergic connection among the DRN, the CeA, and the ACC, but also demonstrated that our device is feasible for multi-site implantation in conjunction with a microdialysis system, allowing the simultaneous multi-modal observation of different regions in the brain.journal articl
A novel eukaryotic RdRP-dependent small RNA pathway represses antiviral immunity by controlling an ERK pathway component in the black-legged tick
No full textSmall regulatory RNAs (sRNAs) are involved in antiviral defense and gene regulation. Although roles of RNA-dependent RNA Polymerases (RdRPs) in sRNA biology are extensively studied in nematodes, plants and fungi, understanding of RdRP homologs in other animals is still lacking. Here, we study sRNAs in the ISE6 cell line, which is derived from the black-legged tick, an important vector of human and animal pathogens. We find abundant classes of ~22nt sRNAs that require specific combinations of RdRPs and sRNA effector proteins (Argonautes or AGOs). RdRP1-dependent sRNAs possess 5’-monophosphates and are mainly derived from RNA polymerase III-transcribed genes and repetitive elements. Knockdown of some RdRP homologs misregulates genes including RNAi-related genes and the regulator of immune response Dsor1. Sensor assays demonstrate that Dsor1 is downregulated by RdRP1 through the 3’UTR that contains a target site of RdRP1-dependent repeat-derived sRNAs. Consistent with viral gene repression by the RNAi mechanism using virus-derived small interfering RNAs, viral transcripts are upregulated by AGO knockdown. On the other hand, RdRP1 knockdown unexpectedly results in downregulation of viral transcripts. This effect is dependent on Dsor1, suggesting that antiviral immunity is enhanced by RdRP1 knockdown through Dsor1 upregulation. We propose that tick sRNA pathways control multiple aspects of immune response via RNAi and regulation of signaling pathways.journal articl
Rational design of alcoholic fermentation targeting extracellular carbon
Get PDFBreeding yeast strains for industrial alcoholic fermentation requires laborious screening due to the lack of in vivo modification strategies. Here we show that quiescence-specific cell wall thickening via synthesis of a major component, 1,3-β-glucan, critically antagonizes cellular fermentation ability by sequestering the available cytoplasmic carbon sources. This study provides insights into glycolytic control and reports an effective and reliable rational fermentation design.journal articl