32970 research outputs found
Sort by
A case report of pulmonary amyloidosis recognized by detection of AA amyloid exclusively in alveolar macrophages
Get PDFAmyloidosis is a rare condition in which tissue deposits of inert fibrillar protein result in organ damage and dysfunction. There are several types of amyloid fibrils. Some of the most common forms are AL (amyloid light chain) protein and AA (amyloid-associated) type of amyloid fibril protein. Pulmonary amyloidosis is relatively common but is usually asymptomatic. Thus, the diagnosis may be easily overlooked. A 78-year-old male with a history of multiple myeloma followed by systemic amyloidosis presented with abnormal chest CT showing diffuse interlobular thickening in the whole lung field with bilateral pleural effusion. Bronchoalveolar lavage and transbronchial biopsy were performed. Due to the patient’s poor condition and hemorrhage, only one fragment was available from forceps biopsy. Histologically, there was no amyloid deposition in the lung parenchyma;
however, some histiocytes showed eosinophilic granular contents which prompted us to perform additional staining. The cytoplasmic material turned to be positive with direct fast scarlet (DFS) staining and AA amyloid immunostaining. Similar macrophages with AA amyloid were also found in the bronchoalveolar fluid. We experienced a case with AA amyloidosis affecting the lung diagnosed by the presence of intracytoplasmic amyloid in alveolar macrophages. The microscopic changes were so subtle that they may be overlooked. Recognition of amyloid deposition in alveolar macrophages may be an important clue to diagnose pulmonary amyloidosis. Such finding is of particular significance in the small-sized specimens, such as biopsies and cytologic smears
Quinone-based antibody labeling reagent for enzyme-free chemiluminescent immunoassays. Application to avidin and biotinylated anti-rabbit IgG labeling
No full textChemiluminescence-enzyme immunoassays make it possible to measure trace components with high sensitivity and selectivity due to the high specificity of the antigen-antibody reaction and the high sensitivity of chemiluminescence assays. However, using an enzyme-labeled antibody suffers from many problems such as low reproducibility due to the instability of the enzyme and inhibition of antigen-antibody reaction due to its steric effect. Therefore, herein we report an innovative non-enzymatic chemiluminescence immunoassays labeling reagent through using quinone as a signal-generating tag coupled with biotin as a binder, to overcome enzymatic labeling problems. Biotinylated-1,4-naphthoquinone (biotin-NQ) was synthesized and characterized and it could produce long-lasting chemiluminescence upon mixing with dithiothreitol and luminol based on the redox cycle of quinone. Biotin-NQ showed exceptional stability towards different stress factors that may be encountered during performing the immunoassay such as high temperatures, highly acidic and alkaline conditions, and repeated freeze-thaw cycles. On the other hand, all these conditions lead to decreased labeling enzyme reactivity due to possible denaturation of its protein structure. Finally, the measurement of the biotin-labeled antibody was successfully performed using biotin-NQ and avidin. As a result, the antibody could be detected down to 25.7 nM which is 2.5 times sensitive than biotin-HRP chemiluminescence-enzyme immunoassays. Moreover, our method was applied successfully for determination of avidin using immobilized biotinylated antibody and biotin-NQ, which simulates immunoassays. Avidin could be detected down to 23.4 nM with excellent linearity (r = 0.996). Accordingly, our developed reagent, biotin-NQ, could be used as a universal highly stable, cost-effective, and steric free non-enzymatic label for immunoassays
Study on High Efficiency and Effect of Stray Capacitance for Soft Switching Power Supply Connected Commercial Power Source
No full text長崎大学学位論文 [学位記番号]博（工）甲第78号 [学位授与年月日]令和2年2月19
The view of nature and human view in Chinese literature by Japanese modern Chinese scholars
No full text長崎大学学位論文 [学位記番号]博（水・環）甲第61号 [学位授与年月日]令和2年3月19
Don't rely too much on trees: Evidence from flood mitigation in China
No full textCombining a popular flood disaster dataset with climate data and satellite land cover data from China, this paper estimates how forests mitigate the frequency of flooding, resulting in two major findings. First, we confirm that an increase in forest area mitigates the possibility of flood occurrence even after controlling for socioeconomic and meteorological variables and time-invariant individual effects. Second, broadleaf trees and mixed-tree forests have a flood mitigation effect, whereas coniferous trees do not; these results are robust against alternative model specifications. This paper newly corroborates the concept of ecosystem-based disaster risk reduction. While there is an emerging consensus that ecosystems can mitigate natural disasters, there is limited evidence on how ecosystems mitigate disasters. To the best of the authors' knowledge, this study is the first to show that the type of forest is critical for mitigating floods in a rigorous econometric way (survival analysis) spanning numerous areas of interest