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School of Global Humanities and Social Sciences, Nagasaki University
No full textJapanese Immigration Control Regime established in 1990 necessitated multiplication of diverse side doors. New law of 2018 admits legal immigrant workers on the basis of their specified skills. This article will examine the ambiguity of the concept of “skills” and will show the contradictory aspects of newly established “specified skills” visa
Expression of a Constitutively Active Form of Hck in Chondrocytes Activates Wnt and Hedgehog Signaling Pathways, and Induces Chondrocyte Proliferation in Mice
Get PDFRunx2 is required for chondrocyte proliferation and maturation. In the search of Runx2 target genes in chondrocytes, we found that Runx2 up-regulated the expression of hematopoietic cell kinase (Hck), which is a member of the Src tyrosine kinase family, in chondrocytes, that Hck expression was high in cartilaginous limb skeletons of wild-type mice but low in those of Runx2–/– mice, and that Runx2 bound the promoter region of Hck. To investigate the functions of Hck in chondrocytes, transgenic mice expressing a constitutively active form of Hck (HckCA) were generated using the Col2a1 promoter/enhancer. The hind limb skeletons were fused, the tibia became a large, round mass, and the growth plate was markedly disorganized. Chondrocyte maturation was delayed until E16.5 but accelerated thereafter. BrdU-labeled, but not terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL)-positive, chondrocytes were increased. Furthermore, Hck knock-down reduced the proliferation of primary chondrocytes. In microarray and real-time RT-PCR analyses using hind limb RNA from HckCA transgenic mice, the expression of Wnt (Wnt10b, Tcf7, Lef1, Dkk1) and hedgehog (Ihh, Ptch1, and Gli1) signaling pathway genes was upregulated. These findings indicated that Hck, whose expression is regulated by Runx2, is highly expressed in chondrocytes, and that HckCA activates Wnt and hedgehog signaling pathways, and promotes chondrocyte proliferation without increasing apoptosis
肝移植後Ｃ型肝炎ウィルスに再感染した患者におけるNS5A領域薬剤耐性関連基質の変化のDeep sequenceでの解析
Get PDF長崎大学学位論文 [学位記番号]博（医歯薬）甲第1210号 [学位授与年月日]令和2年3月4
日本の土壌伝播蠕虫は制圧されているのか？分子生物学的手法を用いた日本の土壌伝播蠕虫感染状況調査結果
Get PDF長崎大学学位論文 [学位記番号]博（医歯薬）甲第1250号 [学位授与年月日]令和2年3月31
Studies on the development and maintenance mechanisms of winter planktonic diatom blooms in the inner western part of Ariake Bay and its contribution to nutrient dynamics in tidal flat sediments
No full text長崎大学学位論文 [学位記番号]博（水・環）乙第8号 [学位授与年月日]令和2年2月19
Protective effects of a cystine and theanine mixture against acute radiation injury in rats
No full textThis study aims to examine the effects of cystine and theanine (CT), which increases glutathione biosynthesis, on the survival rate and acute radiation injury of the small intestine and bone marrow using a rat model. CT pre-treatment (280 mg/kg for 5 days) significantly improved weight loss and survival rate of rats as compared with the control group after 5 Gy. CT pre-treatment significantly increased the rate of mucosa and crypt length, and decreased the number of apoptotic cells, TUNEL and cleaved caspase-3 positive cells, while increasing the number of mitotic cells and Ki-67 positive cells in jejunal crypts and villi compared to control rats post-irradiation. CT also suppressed bone marrow cell loss and reduced the number of apoptotic cells in bone marrow. These results suggest a protective effect of CT pre-treatment for acute injury after irradiation through apoptosis inhibition and increased proliferative activity in jejunal crypt cells and bone marrow cells