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The L-DBF vaccine cross protects mice against different serotypes after prior exposure to the pathogen
No full textShigellosis is endemic to low- and middle-income regions of the world where children are especially vulnerable. In many cases, there are pre-existing antibodies in the local population and the effect of prior exposure should be considered in the development and testing of vaccines against infection. Our study shows that L-DBF-induced immune responses are not adversely affected by prior exposure to this pathogen. Moreover, somewhat different cytokine profiles were observed in the lungs of vaccinated mice not having been exposed to , suggesting that the immune responses elicited by infection and L-DBF vaccination follow different pathways
Microgravity Spherical Droplet Evaporation and Entropy Effects
No full textRecent efforts to understand low-temperature combustion (LTC) in internal combustion engines highlight the need to improve chemical kinetic mechanisms involved in the negative temperature coefficient (aka cool flame) regime. Interestingly, microgravity droplet combustion experiments demonstrate this cool flame behavior, allowing a greater focus on chemistry after buoyancy, and the corresponding influence of the conservation of momentum is removed. In Experimental terms, the LTC regime is often characterized by a reduction in heat transfer losses. Novel findings in this area demonstrate that lower entropy generation, in conjunction with diminished heat transfer losses, could more definitively define the LTC regime. As a result, the simulation of the entropy equation for spherical droplet combustion under microgravity could help us to investigate fundamental LTC chemical kinetic pathways. To provide a starting point for researchers who are new to this field, this effort first provides a comprehensive and detailed derivation of the conservation of entropy equation using spherical coordinates and gathers all relevant information under one cohesive framework, which is a resource not readily available in the literature. Subsequently, the well-known d law analytical model is determined and compared to experimental data that highlight shortcomings of the law. The potential improvements in the d law are then discussed, and a numerical model is presented that includes entropy. The resulting codes are available in an online repository to ensure that other researchers interested in expanding this field of work have a fundamental starting point
The Place of World History in South Dakota’s Failed 2021 Social Studies Standards Revision Process
No full textAn alarming feature of public education in the United States since 2020 has been a highly coordinated and fiercely combative movement from the right seeking to root out and remove Critical Race Theory (CRT) in the schools. CRT is a legal theory developed decades ago that is only rarely a part of publicly funded curricula at the primary or secondary level. In practice, the term CRT is used in a nebulous fashion that 1 bears little resemblance to the theory it purports to oppose. This movement is a reaction to the provocative 1619 Project published by the New York Times specifically, and to movements for racial justice such as Black Lives Matter more broadly. Responding to 2 the 1619 Project in September of 2020, the Trump administration created the 1776 Commission to historically justify American exceptionalism, largely by downplaying the centrality of slavery and by highlighting the significance of American founding ideals as expressed in the late 18th century. Over the past two years dozens of state legislatures 3 have passed legislation banning CRT or the teaching of ‘divisive concepts’ in public schools.
Most of the scholarly and public attention surrounding the anti-CRT movement has rightly focused on American history education. World history, the second-most 5 important history course taught in U.S. public schools, is only rarely considered within this context. Using the contentious standards revision process of 2021 in South Dakota 6 as a case study, this article will trace how the strident nationalism undergirding the anti-CRT movement can affect world history curricula
Physical and Performance Characteristics of 3×3 Professional Male Basketball Players
Get PDFDespite exponential growth in popularity over the last decade and recently becoming an Olympic sport, the amount of scientific literature focused on depicting a profile of successful 3×3 basketball players is sparse. Thus, the purpose of this study was to present the physical and performance characteristics of professional 3×3 male basketball players and how they differ between elite and non-elite athletes. The anthropometrics, vertical jump, agility, and sprint performance parameters collected from ten players during regular training sessions were (x¯
± SD): height (193.7 ± 4.5 cm), weight (89.2 ± 4.1 cm), wingspan (196.5 ± 5.2 cm), squat jump (43.5 ± 4.6 cm), countermovement jump with (53.3 ± 4.4 cm) and without an arm swing (46.3 ± 4.0 cm), reactive strength index (2.4 ± 0.3 m/s), t-test (10.3 ± 0.3 s), 505 drill (2.4 ± 0.2 s), 10 m sprint (1.5 ± 0.1 s), 30 m sprint (4.0 ± 0.3 s), shuttle run (27.7 ± 1.7 s), and bench press (98.2 ± 10.0 kg) and back squat (139.5 ± 17.6 kg) one repetition maximum. Additionally, the average and maximal heart rate (HR) responses during simulated games were 160.6 ± 8.0 and 188.5 ± 6.3 bpm, with players spending 6.3 ± 4.2, 11.4 ± 5.2, 13.9 ± 3.5, 26.4 ± 10.4, and 42.1 ± 10.0% of the total time in HR Zones 1–5, respectively. Interestingly, no statistically significant differences in the aforementioned physical and performance parameters were noted between elite and non-elite players. Overall, the findings of the present study provide coaches, sports scientists, and strength and conditioning practitioners with information that can aid in the athlete selection process, detection of areas for further improvement, and development of training regimens that resemble 3×3 basketball on-court competitive demands
Wearable Sensing System for NonInvasive Monitoring of Intracranial BioFluid Shifts in Aerospace Applications
Get PDFThe alteration of the hydrostatic pressure gradient in the human body has been associated with changes in human physiology, including abnormal blood flow, syncope, and visual impairment. The focus of this study was to evaluate changes in the resonant frequency of a wearable electromagnetic resonant skin patch sensor during simulated physiological changes observed in aerospace applications. Simulated microgravity was induced in eight healthy human participants (n = 8), and the implementation of lower body negative pressure (LBNP) countermeasures was induced in four healthy human participants (n = 4). The average shift in resonant frequency was −13.76 ± 6.49 MHz for simulated microgravity with a shift in intracranial pressure (ICP) of 9.53 ± 1.32 mmHg, and a shift of 8.80 ± 5.2097 MHz for LBNP with a shift in ICP of approximately −5.83 ± 2.76 mmHg. The constructed regression model to explain the variance in shifts in ICP using the shifts in resonant frequency (R2 = 0.97) resulted in a root mean square error of 1.24. This work demonstrates a strong correlation between sensor signal response and shifts in ICP. Furthermore, this study establishes a foundation for future work integrating wearable sensors with alert systems and countermeasure recommendations for pilots and astronauts
Formulation Studies to Develop Low-cost, Orally-delivered Secretory IgA Monoclonal Antibodies for Passive Immunization Against Enterotoxigenic Escherichia coli (Dataset)
No full textEnterotoxigenic Escherichia coli (ETEC) is a common cause for diarrheal infections in children in low- and middle-income countries (LMICs). To date, no ETEC vaccine candidates have been approved. Passive immunization with low-cost, oral formulations of secretory IgA (sIgA) against ETEC is an alternative approach to protect high-risk populations in LMICs. Using a model sIgA monoclonal antibody (anti-LT sIgA2-mAb), the stability profiles of different formulations were assessed during storage and in in vitro digestion models (mimicking in vivo oral delivery). First, by employing various physicochemical techniques and an LT-antigen binding assay, three formulations with varying acid-neutralizing capacity (ANC) were evaluated to stabilize sIgA2-mAb during stress studies (freeze-thaw, agitation, elevated temperature) and during exposure to gastric phase digestion. Next, a low-volume, in vitro intestinal digestion model was developed to screen various additives to stabilize sIgA2-mAb in the intestinal phase. Finally, combination of high ANC buffers and decoy proteins were assessed to collectively protect sIgA2-mAb during in vitro sequential (stomach to intestine) digestion. Based on the results, we demonstrate the feasibility of low-cost, ‘single-vial’, liquid formulations of sIgA-mAbs delivered orally after infant feeding for passive immunization, and we suggest future work based on a combination of in vitro and in vivo stability considerations
Simultaneous N-Deglycosylation and Digestion of Complex Samples on S-Traps Enables Efficient Glycosite Hypothesis Generation
Get PDFN-linked glycosylation is an important post-translational modification that is difficult to identify and quantify in traditional bottom-up proteomics experiments. Enzymatic deglycosylation of proteins by peptide:N-glycosidase F (PNGase F) prior to digestion and subsequent mass spectrometry analysis has been shown to improve coverage of various N-linked glycopeptides, but the inclusion of this step may add up to a day to an already lengthy sample preparation process. An efficient way to integrate deglycosylation with bottom-up proteomics would be a valuable contribution to the glycoproteomics field. Here, we demonstrate a proteomics workflow in which deglycosylation and proteolytic digestion of samples occur simultaneously using suspension trapping (S-Trap). This approach adds no time to standard digestion protocols. Applying this sample preparation strategy to a human serum sample, we demonstrate improved identification of potential N-glycosylated peptides in deglycosylated samples compared with non-deglycosylated samples, identifying 156 unique peptides that contain the N-glycosylation motif (asparagine-X-serine/threonine), the deamidation modification characteristic of PNGase F, and an increase in peptide intensity over a control sample. We expect that this rapid sample preparation strategy will assist in the identification and quantification of both known and potential glycoproteins. Data are available via ProteomeXchange with the identifier PXD037921
KU-phoria! A collection of stories about KU traditions, KU Info and KU basketball
Get PDFIn his fourth decade of service to the University of Kansas, Curtis Marsh has developed an obsessive love for his school. As an undergrad, he led one of the first organized camping groups for KU basketball games and a nationally prominent student entrepreneurship club. In his early career at KU, he helped develop a technology business incubator which has become KU Innovation Park. He also co-founded the Osher Lifelong Learning Institute before serving as Director of KU Info for 15 years. He was the first and only director of the DeBruce Center: the shrine for the original rules of basketball. He now serves as a director of development at KU Endowment. The common thread through all these activities is his love for KU and his desire to share that love with the entire Jayhawk Nation
Search for light Higgs bosons from supersymmetric cascade decays in pp collisions at √=13TeV
Get PDFA search is reported for pairs of light Higgs bosons (H1) produced in supersymmetric cascade decays in final states with small missing transverse momentum. A data set of LHC pp collisions collected with the CMS detector at √=13TeV and corresponding to an integrated luminosity of 138fb−1 is used. The search targets events where both H1 bosons decay into pairs that are reconstructed as large-radius jets using substructure techniques. No evidence is found for an excess of events beyond the background expectations of the standard model (SM). Results from the search are interpreted in the next-to-minimal supersymmetric extension of the SM, where a “singlino” of small mass leads to squark and gluino cascade decays that can predominantly end in a highly Lorentz-boosted singlet-like H1 and a singlino-like neutralino of small transverse momentum. Upper limits are set on the product of the squark or gluino pair production cross section and the square of the branching fraction of the H1 in a benchmark model containing almost mass-degenerate gluinos and light-flavour squarks. Under the assumption of an SM-like branching fraction, H1 bosons with masses in the range 40–120GeV arising from the decays of squarks or gluinos with a mass of 1200–2500GeV are excluded at 95% confidence level
Using Social Network Analysis to Link Community Health and Network Strength
Get PDFSocial network analysis (SNA) is a technique used to analyze social networks, whether it be composed of people, organizations, physical locations, or objects. It is being increasingly applied across a variety of sectors to gain insight into patterns of behavior and connectivity, the flow of information and behaviors, and to track and predict the effectiveness of interventions or programs. A key area associated with network strength using SNA is the health and wellness of individuals and communities. Both network strength and health and wellness are measured in many ways, which can obfuscate the association, so more consistency and further research is required. Despite this, the existing research using SNA to link characteristics of social networks to health and wellness find that stronger, more connected networks tend to be associated with better health outcomes. These results also present opportunities and insights for effective program implementation in response to disasters, to increase resilience, and to improve outcomes for individuals and communities