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“On an island by myself”: implications for the inclusion of autistic students in self-contained classrooms in public elementary schools
No full textIntroduction
Autistic students have limited access to inclusive classes and activities in their schools. Principals and special education teachers who directly teach and administer programs for autistic elementary students can offer critical insight into factors, such as educators’ attitudes, that may impact inclusive opportunities in schools. These attitudes may serve as barriers to or facilitators of promoting an inclusive school setting.
Methods
Semi-structured interviews with 26 elementary school principals and 26 special education teachers explored their experiences implementing evidence-based practices for autistic students (pivotal response training, discrete trial training, and visual schedules) in 26 self-contained classrooms in the United States. Autism-specific culture and inclusion emerged as a theme, which was analyzed for this paper.
Results
An inductive approach to thematic analysis revealed principals’ and special education teachers’ perspectives regarding the “autism-specific culture” in the school, including attitudes towards and inclusion of autistic students in self-contained classrooms in the broader school environment. Analysis of text related to “autism-specific culture” detailed aspects of inclusion, factors (i.e., barriers and facilitators) affecting inclusion, principals’ and special education teachers’ attitudes towards autistic students placed in self-contained classrooms, attitudes of other school staff towards teachers in self-contained classrooms, and recommendations to support an inclusive school environment for autistic students.
Discussion
Results suggest that valuing “equal” access to classes and activities for autistic students in self-contained classrooms may not be sufficient for promoting an inclusive school environment, Educators may benefit from targeted strategies to facilitate inclusion. Strategies range from supporting educators’ attitudes and knowledge of autism to shifting physical aspects of the school environment (e.g., location of classrooms). Additional implications for supporting the true inclusion (i.e., inclusion that goes beyond physical inclusion) involves of autistic students in self-contained classrooms schools are discussed
Are we saving water? Simple methods for assessing the effectiveness of groundwater conservation measures
Get PDFSubstantial storage reductions by irrigation pumping in many of the world’s major aquifers jeopardize future food production. As a result, new conservation measures are being utilized to reduce pumping and extend aquifer lifespans. The key question is how effective are these practices in attaining true water conservation (i.e., water use reduction) for a given area? Relationships between pumping and precipitation help provide an answer, as precipitation explains most of the variation in annual irrigation water use for aquifers in semi-arid to sub-humid climates when surface water supplies are limited. Our objective is to utilize correlations between radar precipitation and irrigation groundwater use at a range of spatial scales to assess the effectiveness of conservation approaches in the High Plains aquifer in the central USA. Linear regressions between pumping and precipitation for a conservation area established in 2013 in northwest Kansas indicate that water use and water use per irrigated area were over 27 % less and 25 % less, respectively, during 2013–2021 compared to the same climatic conditions during 2005–2012. Similar regressions found over a 38 % reduction and 23 % reduction in irrigation water use and use per irrigated area, respectively, during 2018–2021 compared to the same conditions during 2005–2017 in a west-central Kansas county with conservation areas. A decrease in irrigated area accounted for most of the difference between these reductions. Higher R2 values after conservation area establishment imply that irrigation tracks precipitation better due to use of soil moisture sensors and other measures as part of increased irrigation efficiency and enhanced water management. The precipitation and water use relationships, which are statistically significant for a wide range of spatial scales, have great potential for assessing the effectiveness of conservation practices in areas with high-quality water use and precipitation data
Urbanization as a limiter and catalyst of watershed-scale sediment transport: Insights from probabilistic connectivity modeling
Get PDFThe conversion of rural lands to urban areas exerts considerable influence on the hydrologic processes governing sediment transport at the watershed scale. While the effects of urbanization on hydrology have been well-studied, the corresponding impact to the spatial and temporal variability of sediment detachment, transport, and connectivity is less certain. To address this knowledge gap, we apply process-based hydrologic simulation, probabilistic connectivity modeling, and in situ turbidity sensing to five watersheds positioned along a steep land use gradient in Kansas, USA. Connectivity modeling results show that urbanization systematically decreases the maximal extent of watershed-scale connectivity on the wettest days of the study period, from 51 % in the most rural watershed to 28 % in the most urban watershed. On the other hand, urbanization focuses sediment transport into fewer, more frequently wetted pathways, such as roadway drainage networks, which are activated 3.5 times more frequently than the equivalent pathways in rural basins. In this way, urbanization limits maximal connectivity as impervious surfaces indefinitely disconnect source zones from the sediment cascade, but also catalyzes hot spots of connectivity as these same impervious areas generate excess runoff and channel it to drainage systems. The 23.9 ± 4.2 % of days that exhibit watershed-scale functional connectivity account for 85.0 ± 9.5 % of sediment export with most of the export tied to a few highly connected days. Sensing results show that increases in watershed-scale connectivity only translate to larger fluvial sediment loads after a connectivity threshold (the median connected day) has been exceeded, suggesting a transition from functional to structural connectivity control on sediment dynamics after sufficient wetting. This study highlights the role of land use impacts on the sources and mechanisms of sediment transport, which will be an important consideration for land managers as urban areas continue to expand to accommodate global migration patterns
Biodiversity of Philippine marine fishes: A DNA barcode reference library based on voucher specimens
Get PDFAccurate identification of fishes is essential for understanding their biology and to ensure food safety for consumers. DNA barcoding is an important tool because it can verify identifications of both whole and processed fishes that have had key morphological characters removed (e.g., filets, fish meal); however, DNA reference libraries are incomplete, and public repositories for sequence data contain incorrectly identified sequences. During a nine-year sampling program in the Philippines, a global biodiversity hotspot for marine fishes, we developed a verified reference library of cytochrome c oxidase subunit I (COI) sequences for 2,525 specimens representing 984 species. Specimens were primarily purchased from markets, with additional diversity collected using rotenone or fishing gear. Species identifications were verified based on taxonomic, phenotypic, and genotypic data, and sequences are associated with voucher specimens, live-color photographs, and genetic samples catalogued at Smithsonian Institution, National Museum of Natural History. The Biodiversity of Philippine Marine Fishes dataset is released herein to increase knowledge of species diversity and distributions and to facilitate accurate identification of market fishes
Assessing Breast Cancer Molecular Subtypes Using Extracellular Vesicles’ mRNA
No full textThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © 2023 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.3c00624.Extracellular vesicles (EVs) carry RNA cargo that is believed to be associated with the cell-of-origin and thus have the potential to serve as a minimally invasive liquid biopsy marker for supplying molecular information to guide treatment decisions (i.e., precision medicine). We report the affinity isolation of EV subpopulations with monoclonal antibodies attached to the surface of a microfluidic chip that is made from a plastic to allow for high-scale production. The EV microfluidic affinity purification (EV-MAP) chip was used for the isolation of EVs sourced from two-orthogonal cell types and was demonstrated for its utility in a proof-of-concept application to provide molecular subtyping information for breast cancer patients. The orthogonal selection process better recapitulated the epithelial tumor microenvironment by isolating two subpopulations of EVs: EVEpCAM (epithelial cell adhesion molecule, epithelial origin) and EVFAPα (fibroblast activation protein α, mesenchymal origin). The EV-MAP provided recovery >80% with a specificity of 99 ± 1% based on exosomal mRNA (exo-mRNA) and real time–droplet digital polymerase chain reaction results. When selected from the plasma of healthy donors and breast cancer patients, EVs did not differ in size or total RNA mass for both markers. On average, 0.5 mL of plasma from breast cancer patients yielded ∼2.25 ng of total RNA for both EVEpCAM and EVFAPα, while in the case of cancer-free individuals, it yielded 0.8 and 1.25 ng of total RNA from EVEpCAM and EVFAPα, respectively. To assess the potential of these two EV subpopulations to provide molecular information for prognostication, we performed the PAM50 test (Prosigna) on exo-mRNA harvested from each EV subpopulation. Results suggested that EVEpCAM and EVFAPα exo-mRNA profiling using subsets of the PAM50 genes and a novel algorithm (i.e., exo-PAM50) generated 100% concordance with the tumor tissue
The Building Healthy Eating and Self-Esteem Together for University Students Mobile App to Treat Eating Disorders: User-Centered Research Design and Feasibility Study
Get PDFBackground:
University students are an at-risk group for the development of eating disorders (EDs); however, many college campuses lack sufficient resources to provide ED specialty care. Students report unique reasons for not seeking ED treatment, including the desire to solve the problem on their own (eg, seeking help from friends, self-medicating, or waiting to see if their problems improve), inability to afford treatment, lack of time to participate in the treatment, fear of seeing their primary care physician, and lack of recognition of their issues as an ED. Mobile health (mHealth) apps may be a cost-effective, helpful adjunctive tool to overcome personal and systemic barriers and encourage help seeking.
Objective:
This paper describes the development, usability, and acceptability of the Building Healthy Eating and Self-Esteem Together for University Students (BEST-U) mHealth smartphone app, which is designed to fill critical gaps in access to ED treatment on college campuses.
Methods:
We undertook a 4-phase iterative development process that focused on user-centered design. The 4 phases included needs assessment based on literature reviews, prototype development and initial evaluation in a pilot trial, redesign, and further pilot-testing to assess the usability and acceptability of the final version of the mHealth app. Acceptability and user satisfaction were assessed using an ad hoc survey that ranged from 1 (strongly disagree) to 7 (strongly agree).
Results:
Our needs assessment identified a lack of accessible and affordable treatments for university students. To help meet this need, the BEST-U prototype was designed as an 11-week program that provided interactive, weekly modules that focused on second- and third-wave cognitive behavioral skills. The modules focused on topics such as psychoeducation, reducing thought distortions and body checking, improving body image, interpersonal effectiveness, and behavior chain analysis. The content included interactive quizzes, short answer questions, daily and weekly logs, and surveys completed in the app. BEST-U was paired with brief 25-30 minutes of weekly telehealth coaching sessions provided by a licensed provider or supervised trainee. Pilot-testing revealed minor issues with one module of the app content, which some participants viewed as having low relevance to their experience and therapist concerns about the organization of the app content. These issues were addressed through the removal, addition, and reorganization of BEST-U modules, with the help of therapists-in-training across 2 workshops. The revised version of the BEST-U app had a grand mean acceptability rating of 5.73 out of 7. The participants completed 90.1% (694/770) of the BEST-U modules, indicating high compliance.
Conclusions:
BEST-U is a new, acceptable, and user-friendly mHealth app to help therapists deliver brief, evidence-based cognitive behavioral interventions. Owing to its acceptability and user-friendly nature, BEST-U has high user compliance and holds promise for future implementation and dissemination in university mental health settings
Activity of Delafloxacin and Comparator Fluoroquinolones against Multidrug-Resistant Pseudomonas aeruginosa in an In Vitro Cystic Fibrosis Sputum Model
Get PDFDelafloxacin (DLX) is a recently approved fluoroquinolone with broad activity against common cystic fibrosis (CF) pathogens, including multidrug-resistant Pseudomonas aeruginosa (MDR-Psa). Delafloxacin has been previously shown to have excellent lung and biofilm penetration and enhanced activity at lower pH environments, such as those that would be observed in the CF lung. We analyzed six Psa strains isolated from CF sputum and compared DLX to ciprofloxacin (CPX) and levofloxacin (LVX). Minimum inhibitory concentrations (MICs) were determined for DLX using standard culture media (pH 7.3) and artificial sputum media (ASM), a physiologic media recapitulating the CF lung microenvironment (pH 6.9). Delafloxacin activity was further compared to CPX and LVX in an in vitro CF sputum time-kill model at physiologically relevant drug concentrations (Cmax, Cmed, Cmin). Delafloxacin exhibited 2- to 4-fold MIC reductions in ASM, which corresponded with significant improvements in bacterial killing in the CF sputum time-kill model between DLX and LVX at Cmed (p = 0.033) and Cmin (p = 0.004). Compared to CPX, DLX demonstrated significantly greater killing at Cmin (p = 0.024). Overall, DLX demonstrated favorable in vitro activity compared to alternative fluoroquinolones against MDR-Psa. Delafloxacin may be considered as an option against MDR-Psa pulmonary infections in CF
Genome sequence of Ophryocystis elektroscirrha, an apicomplexan parasite of monarch butterflies: cryptic diversity and response to host-sequestered plant chemicals
Get PDFApicomplexa are ancient and diverse organisms which have been poorly characterized by modern genomics. To better understand the evolution and diversity of these single-celled eukaryotes, we sequenced the genome of Ophryocystis elektroscirrha, a parasite of monarch butterflies, Danaus plexippus. We contextualize our newly generated resources within apicomplexan genomics before answering longstanding questions specific to this host-parasite system. To start, the genome is miniscule, totaling only 9 million bases and containing fewer than 3,000 genes, half the gene content of two other sequenced invertebrate-infecting apicomplexans, Porospora gigantea and Gregarina niphandrodes. We found that O. elektroscirrha shares different orthologs with each sequenced relative, suggesting the true set of universally conserved apicomplexan genes is very small indeed. Next, we show that sequencing data from other potential host butterflies can be used to diagnose infection status as well as to study diversity of parasite sequences. We recovered a similarly sized parasite genome from another butterfly, Danaus chrysippus, that was highly diverged from the O. elektroscirrha reference, possibly representing a distinct species. Using these two new genomes, we investigated potential evolutionary response by parasites to toxic phytochemicals their hosts ingest and sequester. Monarch butterflies are well-known to tolerate toxic cardenolides thanks to changes in the sequence of their Type II ATPase sodium pumps. We show that Ophryocystis completely lacks Type II or Type 4 sodium pumps, and related proteins PMCA calcium pumps show extreme sequence divergence compared to other Apicomplexa, demonstrating new avenues of research opened by genome sequencing of non-model Apicomplexa
Food Insecurity and Rural Populations
No full textPresented at “Topics in Eating Disorder Treatment: Food Insecurity and Rural Populations,” University of Kansas, March 31, 2023. Center for the Advancement of Research on Eating Disorders (CARE): https://care.ku.edu/
Dr. Kelsie Forbush is a Professor of Clinical Child Psychology and the CARE and COPE Lab Director at the University of Kansas
Dr. Hana Zickgraf is an Assistant Professor of Pediatrics at the Emory University School of Medicine
Dr. Kara Christensen is an Assistant Professor in the Psychology Department at the University of Nevada Las Vegas
Dr. Heather Davis is an Assistant Professor in the Psychology Department at Virginia TechIn this panel discussion, Dr. Kelsie Forbush meets with Dr. Hana Zickgraf, Dr. Kara Christensen, and Dr. Heather Davis to discuss how food insecurity and rural populations can affect eating disorder treatment. Topics discussed include prevalence of food insecurity in eating disorders, the feast or famine cycle, higher BMIs, diet culture and the thin ideal, utilizing trauma informed care, resource acquisition, intersectionality, supplements, and measurements to assess food insecurity
Relationships among cost, citation, and access in journal publishing by an ecology and evolutionary biology department at a U.S. university
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The scripts for obtention of data are available at KU ScholarWorks: https://doi.org/10.17161/1808.32587.
The data are available at KU ScholarWorks: https://doi.org/10.17161/1808.32708.Background:
Optimizing access to high-quality scientific journals has become an important priority for academic departments, including the ability to read the scientific literature and the ability to afford to publish papers in those journals. In this contribution, we assess the question of whether institutional investment in scientific journals aligns with the journals where researchers send their papers for publication, and where they serve as unpaid reviewers and editors.
Methods:
We assembled a unique suite of information about the publishing habits of our Department of Ecology and Evolutionary Biology, including summaries of 3,540 journal publications by 35 faculty members. These data include economic costs of journals to institutions and to authors, benefits to authors in terms of journal prestige and citation rates, and considerations of ease of reading access for individuals both inside and outside the university. This dataset included data on institutional costs, including subscription pricing (rarely visible to scholars), and “investment” by scholars in supporting journals, such as time spent as editors and reviewers.
Results:
Our results highlighted the complex set of relationships between these factors, and showed that institutional costs often do not match well with payoffs in terms of benefits to researchers (e.g., citation rate, prestige of journal, ease of access). Overall, we advocate for greater cost-benefit transparency to help compare different journals and different journal business models; such transparency would help both researchers and their institutions in investing wisely the limited resources available to academics