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Akut miyeloid lösemi hastalarında “peroksizom proliferatör aktive edici reseptör-gama” geninin epigenetik düzenlenmesi
No full text2-s2.0-85075076224Objective: Peroxisome proliferator activated receptor gamma (PPAR) is a member of the nuclear hormone receptor family that regulates cellular growth, differentiation and apoptosis. PPAR gamma expression is present in normal bone marrow and peripheral CD34+ cells. There are studies showing that this regulation is decreased in acute myeloblastic leukemia (AML). However, there is no study of whether this reduction is transcriptional or epigenetic. For this purpose we investigated the expression and methylation levels of the PPAR gamma gene in AML and healthy control group. Material and Methods: Thirty-one newly diagnosed cases of AML and 25 healthy individuals were included in the study. Differences in the PPAR gamma gene expression between the study group and the control group were analyzed by real time polymerase chain reaction (RT-PCR) technique. Methylation-specific PCR was performed using the Epitect bisulfite protocol. After primers were designed, bisulfite DNA transformation was performed. The methylation level of the PPAR gamma gene was measured by the Epitect HRM PCR protocol. Results: As a result of RT-PCR, the expression of PPAR gamma gene in AML cases was found to be reduced by about 1.5 times compared to healthy individuals. When the methylation status of the PPAR gamma promoter region was examined by methylation-specific PCR in the cases where the PPAR gamma expression was decreased, methylation in the candidate gene region in AML cases was significantly increased (29% in AML cases and 8% in control group, p<0.001). Conclusion: In our study, the expression of the PPAR gamma gene was significantly reduced in patients with AML. It has also been shown that over-methylation of the promoter region may be responsible for suppression of this gene expression. © 2019 by Türkiye Klinikleri
Atriyal fibrilasyon için monopolara kıyasla bipolar radyofrekans ablasyon işleminin uzun dönem sonuçları
No full textBackground: In this study, we aimed to evaluate the long-term outcomes of monopolar or bipolar radiofrequency ablation concomitant to mitral valve surgery in patients with atrial fibrillation. Methods: We retrospectively evaluated a total of 167 patients (67 males, 100 females; mean age 56.8±6.9 years; range, 48 to 65 years) with atrial fibrillation who underwent monopolar or bipolar radiofrequency ablation concomitant to mitral valve surgery between September 2001 and January 2015. The patients were divided into two groups according to the procedure applied as those undergoing monopolar ablation (group 1, n=68) and those undergoing bipolar ablation (group 2, n=99). All patients were followed by electrocardiogram and 24-h Holter monitoring. Echocardiography was performed before discharge, at three and 12 months postoperatively, and annually thereafter. Left atrial volume index, left atrial diameter, and left ventricular ejection fraction were recorded. Results: There was no significant correlation between the procedure applied and hypertension, hyperlipidemia, diabetes mellitus, chronic obstructive pulmonary disease, history of the cerebrovascular events (p>0.05). The mean preoperative left atrial diameter decreased from 5.3±0.5 cm to 4.9±0.5 cm postoperatively in all patients (p=0.0001). The mean preoperative left atrial volume index decreased from 53.8±0.4 mL/m2 t o 43.7±6.2 m L/m2 i n t he postoperative period (p=0.0001). During follow-up, 61.8% (n=42) of the patients in group 1 and 62.6% (n=62) of the patients in group 2 remained in sinus rhythm. One patient (1.5%) in group 1 and two patients (2.0%) in group 2 developed early postoperative cerebrovascular accident. Conclusion: Monopolar and bipolar ablation methods are safe and effective methods to ensure long-term sinus rhythm. Both procedures do not increase the morbidity risk with very low thromboembolic complication rates.Amaç: Bu çalışmada, atriyal fibrilasyonlu hastalarda mitral kapak cerrahisine ek olarak monopolar veya bipolar radyofrekans ablasyon tedavisinin uzun dönem sonuçları değerlendirildi. Çalışma planı: Eylül 2001-Ocak 2015 tarihleri arasında mitral kapak cerrahisine ek olarak monopolar veya bipolar radyofrekans ablasyon yapılan toplam 167 atriyal fibrilasyonlu hasta (67 erkek, 100 kadın; ort. yaş 56.8±6.9 yıl; dağılım, 48-65 yıl) retrospektif olarak değerlendirildi. Hastalar uygulanan işleme göre monopolar ablasyon yapılanlar (grup 1, n=68) ve bipolar ablasyon yapılanlar (grup 2, n=99) olmak üzere iki gruba ayrıldı. Tüm hastaların takibi elektrokardiyografi ve 24 saatlik Holter monitörizasyonu ile yapıldı. Ekokardiyografi taburculuktan önce ve ameliyat sonrası üç ve 12. ayda ve sonrasında yıllık olarak çekildi. Sol atriyal hacim indeksi, sol atriyal çap ve sol ventrikül ejeksiyon fraksiyonu kaydedildi. Bulgular: Yapılan işlem ve hipertansiyon, hiperlipidemi, diabetes mellitus, kronik obstrüktif akciğer hastalığı ve serebrovasküler olay öyküsü arasında anlamlı bir ilişki bulunamadı (p>0.05). Tüm hastalarda ameliyat öncesi ortalama sol atriyal çap 5.3±0.5 cm’den ameliyat sonrası 4.9±0.5 cm’ye düştü (p=0.0001). Ameliyat öncesi sol atriyal hacim indeksi 53.8±0.4 mL/m2’den ameliyat sonrası dönemde 43.7±6.2 mL/m2’ye geriledi (p=0.0001). Takip döneminde grup 1’deki hastaların %61.8’i (n=42) ve grup 2’deki hastaların %62.6’i (n=62) sinüs ritmindeydi. Grup 1’de bir hastada (%1.5) ve grup 2’de iki hastada (%2.0) ameliyat sonrası erken serebrovasküler olay gelişti. Sonuç: Uzun dönem sinüs ritminin sağlanmasında monopolar ve bipolar ablasyon yöntemleri güvenli ve etkilidir. Her iki işlem de çok düşük tromboembolik komplikasyon oranları ile morbidite riskini artırmaz
Kalp Cerrahisinde Postoperatif Perikardiyal Efüzyonun Önlenmesi İçin Posterior Perikardiyal Pencere Tekniği
No full textABSTRACT INTRODUCTION: The aim was to determine the effectiveness of the posterior pericardial window (PW) technique in preventing the development of pericardial effusion (PE) following coronary artery bypass grafting surgery (CABG). METHODS: Patients undergoing coronary surgery were randomly divided into a control or a PW group. We divided 220 patients randomly into 2 groups, the posterior pericardial window group (n=110) and the control group (n=110). Preoperative, intraoperative and postoperative clinical data were collected retrospectively, including incidence of pericardial tamponade, drainage volume, ventilation time and moderate to large pericardial effusion. Evaluations were completed preoperatively, before discharge, and on postoperative 7 and 30 days including electrocardiography, chest radiography, echocardiography. Postoperative causes of morbidity, the duration of intensive care unit and hospital stay were recorded. RESULTS: There was no significant difference in demographic data between two groups (P>.05). Echocardiography evaluations revealed no significant difference between groups preoperatively; however, before discharge the control group had a significantly higher number of patients with early and late cardiac tamponade compared with the PW group (P.05). Ekokardiyografi incelemeleri ameliyat öncesi gruplar arasında anlamlı bir farklılık yoktu; bununla birlikte, taburcu edilmeden önce, kontrol grubunda, perikardiyal pencere grubuna kıyasla, erken ve geç kardiyak tamponad anlamlı olarak yüksek bulundu (P <.05). Perikardiyal pencere grubunda postoperatif plevral efüzyon insidansı kontrol grubundan anlamlı olarak yüksek olarak tespit edildi. Yeni başlangıçlı atriyal fibrilasyon, kontrollerde perikardiyal pencere grubundan anlamlı olarak daha yaygındı (P <.05). TARTIŞMA ve SONUÇ: Posterior perikardiyal pencere tekniği, ciddi komplikasyonlar olmadan kolay uygulanabilir ve güvenli bir yöntemdir. Bu prosedür, geç kardiyak tamponadı ve CABG hastalarında ölümcül bir komplikasyon olabilen efüzyonla ilişkili atriyal fibrilasyonu azaltabilir
Ebelik ve Hemşirelik Öğrencilerinin Premenstrual Sendrom ve Nonfarmakolojik Yöntemlerine İlişkin Bilgi ve Tutumlarının Değerlendirilmesi
No full text1. ÖZET
Ebelik ve Hemşirelik Öğrencilerinin Premenstrual Sendrom ve Nonfarmakolojik Yöntemlerine İlişkin Bilgi ve Tutumlarının Değerlendirilmesi Öğrencinin Adı: NagihanTUFAN
Danışmanı: Dr. Öğr. Üyesi Anita KARACA
Anabilim Dalı: Ebelik
Amaç: Bu çalışma, Ebelik ve Hemşirelik öğrencilerinin premenstrual sendrom ve nonfarmakolojik ağrı yöntemlerine ilişkin bilgi ve tutumlarını değerlendirmek ve etkileyen faktörleri belirlemek amacıyla gerçekleştirildi.
Gereç ve Yöntem: Bu araştırma tanımlayıcı nitelikte bir araştırmadır. Araştırma bir vakıf üniversitesinin Ebelik ve Hemşirelik bölümlerinde 19.12.2018-24.12.2018 tarihleri arasında yapılmıştır. Araştırmanın örneklemini bu tarihlerde okulda olan ve araştırmaya katılmayı kabul eden toplam 172 kız öğrenci oluşturmuştur. Araştırma veriler “Öğrenci Bilgi Formu” ve “Premenstrual Sendrom Ölçeği” kullanılarak toplanmıştır. “Premenstrual Sendrom Ölçeği” 9 alt boyut 44 ifadeden oluşmaktadır. Bulgular: Çalışmaya katılan öğrencilerin %18,6’sı Ebelik, %81,4’ü Hemşirelik öğrencisidir. Öğrencilerin %72,1’inin geliri giderini karşılamakta ve %53,5’i ailesi ile birlikte yaşamaktadır. Öğrencilerin %78,5’inin düzenli adet gördüğü, %44,8’inin dismenore yaşadığı, %76,2’sinin premenstrual dönemde öncelikli olarak nonfarmakolojik yöntemleri kullandığı ve en fazla (%75,6) sıcak uygulama yaptıkları belirlenmiştir. Ayrıca, %25,2’si ağrı geçmeyince farmakolojik yönteme başvurduğunu belirtmiştir. Premenstrual Sendrom Ölçeği toplam ortalama değeri 139,000±36,607 (min.51-max.220)’dir. Bu ölçeğe ilişkin en yüksek alt boyutun (23,256±6,600) “Depresif Duygulanım” ile ilgili olduğu bulunmuştur.
Sonuç: Bu çalışmada, öğrencilerde premenstrual sendromun yüksek olduğu bulunmuştur. Öğrenci ebe ve hemşirelerin büyük çoğunluğu premenstrual dönemde nonfarmakolojik yöntemleri kullanmaktadır. Bu dönemde en fazla yaşadıkları sorun olan depresif duygulanıma ilişkin önlemlerin alınması önerilebilir.
2. SUMMARY
Evaluation of Knowledge and Attitudes about Premenstrual Syndrome and Nonpharmacological Methods of Midwifery and Nursing Students
Name of Student: Nagihan TUFAN
Supervisor: Dr. Lecturer. Member Anita KARACA
Department: Midwifery
Objective: The aim of this study was to evaluate the knowledge and attitudes of the -Midwifery and Nursing students associated with premenstrual syndrome and nonpharmacological pain methods and to determine the factors affecting them.
Materials and Methods: This research is a descriptive study. The research was conducted in Nursing and Midwifery departments of a foundation university between 19.12.2018-24.12.2018. The sample of the study consisted of 172 female students who were in school at this time and agreed to participate in the research. The resarch datas were collected by using ‘‘Student Information Form’’ and ‘‘Premenstrual Syndrome Scale’’ which consists of 9 sub-dimensions and 44 expressions.
Results: 18.6% midwifery and 81.4% nursing students participated in the study. 72.1% of the students meet their expenses and 53.5% of them live with their family. It was determined that 78.5% of the students had regular menstrual periods, 44.8% of them had dysmenorrhea, and 76.2% of them used nonpharmacological methods in the premenstrual period and made the most (75.6%) hot application. In addition, 25.2% stated that he had applied to the pharmacological method without pain. The total average value of Premenstrual Syndrome Scale was 139.000 ± 36.607 (min.51- max.220). The highest sub-dimension of this scale (23.256 ± 6.600) was found to be related to “DepressiveAffection”.
Conclusion: In this study, the total score of premenstrual syndrome was found to be below the mean value of the students. Most of the midwives and nurses used nonpharmacological methods in the premenstrual period. It may be advisable to take measures related to depressive affection which is the most common problem in this period
Which dialysis modality is better for the heart?
No full textPubMed ID: 30874518[No abstract available
Impact of the odors of vanilla extract and breast milk on the frequency of apnea in preterm neonates
No full textAim: We developed this study using an experimental design to determine the impact of the odors of vanilla extract and breast milk on apnea frequency in preterm neonates. Materials and methods: We obtained the study data from 42 preterm neonates (16 vanilla, 13 breast milk, 13 control group) who had been admitted to the Neonatal Intensive Care Unit of Istanbul University meeting the case selection criteria. All groups were monitored on the first day without any intervention. On the second and third days, breast milk was placed in the incubators of the babies in the breast milk group and the sterile gauze that was vanilla-impregnated was placed in the incubators of the babies in the vanilla group. On the fourth day, the babies were watched without any interference. Results: On the second, third and fourth days of the study, we determined that apnea frequency in the vanilla group neonates was significantly lower (P <.00) as compared to the breast milk and control groups. Conclusion: Using vanilla extract in neonates suffering from apnea to reduce its frequency is an effective method. © 2019 Japan Academy of Nursing Scienc
Scoring Systems and Postoperative Outcomes in Pediatric Liver Transplantation
No full textPurpose: The aim of this study is to investigate the effects of risk scores (Pediatric End-stage Liver Disease [PELD], Child-Turcotte-Pugh [CTP], and Pediatric Risk of Mortality [PRISM-III]) of pediatric liver transplant patients on the postoperative period. Method: Seven cadaveric and 45 living donors, totaling 52 pediatric liver transplantation (LT) patients, were reviewed retrospectively. PELD and CTP scores were calculated based on data at hospital admission. PRISM-III score was calculated from data during the first 24 hours of intensive care unit (ICU) admission. Hospital length of stay (LOS), ICU LOS, patients who developed acute kidney injury (AKI), requirement for inotropic-vasopressor therapy, hospital mortality, long-term mortality, duration of mechanical ventilation, metabolic disease, and demographic features were documented.For CTP score, class C was defined as high, and A and B as low. Cutoff values of PELD and PRISM-III scores were detected by using receiver operating characteristic curves. According to these cutoff values, patients were divided into 2 groups as high and low for each score. Documented data was analyzed and compared in groups for each score. Results: Hospital LOS was significantly longer in the high-PELD (P = .01) and high-CTP (P = .01) groups. ICU LOS was significantly longer in the high-PRISM-III group (P = .01). Requirement for inotropic-vasopressor therapy was significantly higher in the high-PELD (P = .04) and high-CTP (P = .04) groups. Conclusion: Hemodynamic instability and long hospital LOS can be expected in pediatric post-LT patients with high PELD or CTP scores; there is also the risk that AKI maybe higher for high-PELD score patients. Unexpectedly, the PRISM-III score did not have any correlation with the severity of physiological condition and mortality. © 2019 Elsevier Inc
IDENTIFICATION OF OXA-GENE IN ACINETOBACTER BAUMANNII ISOLATES OBTAINED FROM CLINICAL SPECIMENS AND THE CLONALITY BETWEEN THESE ISOLATES
No full textAtik, Bulent/0000-0002-6876-2963; Baylan, Orhan/0000-0002-6529-7824WOS: 000508954100007Objective: Our study aimed to reveal the OXA-like carbapenernase genes and molecular epidemiological relationship in A. baumannii isolates and compare antibiotic susceptibility status-resistance genes-Pulsed Field Gel Electrophoresis (PFGE) patterns. Material and Method: A total of 100 A. baumannii isolates obtained from several clinical specimens were examined by VITEK2 automated identification system (Biomerieux, France) for the antibiotic susceptibility profile, by multiplex polymerase chain reaction (PCR) method for OXA-type beta-lactamuses and by PFGE method for the clonal relationship. Results: 57% of the isolates were obtained from respiratory system specimens. Among all the antibiotics, colistin was the most effective agent with 100% sensitivity, followed by tigecycline with 93%. All of the isolates were resistant to piperacillin, piperacillinitazobaaam and ciprofloxacin. Resistance against both imipenem and meropenem were detected as 95%. OXA-51 gene was found in all and oxil-23 gene was found in 92 (92%) of the isolates. None of the isolates had OXA-24 or OXA-58 genes. We identified 19 different clonal clusters among 100 isolates by PFGE method. We revealed that some of the clones were clustered in a certain period of time, and this was supported by the antibiogram results and OXA gene profiling. Conclusion: Our study identified high rates OXA-23 gene locus positivity, presented the current clonal similarity and time relationship among the clonal clusters. These results emphasize the importance of molecular epidemiological methods as well as standard infection control programs to prevent spreading of A. baumannii
International prevalence and risk factors evaluation for drug-resistant Streptococcus pneumoniae pneumonia
No full textPubMed: 31299410Objective: Streptococcus pneumoniae is the most frequent bacterial pathogen isolated in subjects with Community-acquired pneumonia (CAP) worldwide. Limited data are available regarding the current global burden and risk factors associated with drug-resistant Streptococcus pneumoniae (DRSP) in CAP subjects. We assessed the multinational prevalence and risk factors for DRSP-CAP in a multinational point-prevalence study. Design: the prevalence of DRSP-CAP was assessed by identification of DRSP in blood or respiratory samples among adults hospitalized with CAP in 54 countries. Prevalence and risk factors were compared among subjects that had microbiological testing and antibiotic susceptibility data. Multivariate logistic regressions were used to identify risk factors independently associated with DRSP-CAP. Results: 3,193 subjects were included in the study. the global prevalence of DRSP-CAP was 1.3% and continental prevalence rates were 7.0% in Africa, 1.2% in Asia, and 1.0% in South America, Europe, and North America, respectively. Macrolide resistance was most frequently identified in subjects with DRSP-CAP (0.6%) followed by penicillin resistance (0.5%). Subjects in Africa were more likely to have DRSP-CAP (OR: 7.6; 95%CI: 3.34-15.35, p<0.001) when compared to centres representing other continents. Conclusions: This multinational point-prevalence study found a low global prevalence of DRSP-CAP that may impact guideline development and antimicrobial policies. © 2019Quality Enhancement Research Initiative, QUERI: HX002263-01A1 U.S. Department of Veterans Affairs, VAThis project was unfunded. However, Nilam Soni's time is partially funded by the Department of Veterans Affairs, Quality Enhancement Research Initiative (QUERI) Partnered Evaluation Initiative Grant ( HX002263-01A1 ). the content is solely the responsibility of the authors and does not necessarily represent the official views of the Department of Veterans Affairs, nor the United States Government
Electroencephalographic Differences in Kindling-Induced Afterdischarges Among Genetic Absence Rat Models and Wistar Rats
No full text33rd International Epilepsy Congress -- JUN 22-26, 2019 -- Bangkok, THAILANDWOS: 000493585100287[No abstract available