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Efficacy of adalimumab therapy in experimental rat sclerosing encapsulated peritonitis model
No full textWOS: 000506357400006PubMed: 31686457Aim To investigate the efficacy of adalimumab treatment in an experimental rat sclerosing encapsulated peritonitis (SEP) model. Methods the study involved 40 Wistar albino rats divided into four groups: chlorhexidine (CH) group, control group, CH + adalimumab group, and CH + resting group. the control group received normal saline intraperitoneally (i.p.). Other groups received 0.1% CH gluconate, 15% ethanol, and normal saline mixture i.p. for three weeks in order to induce SEP. CH + adalimumab group received 5 mg/kg adalimumab i.p. at the beginning of week 4 and week 6, while CH + resting group was followed-up for three weeks without applying any procedure after the onset of SEP. Rats in groups CH and control group were sacrificed on day 21, and rats in group CH + adalimumab and CH + resting were sacrificed on day 42. All groups were evaluated for peritoneal thickness, inflammation, vascularization, and fibrosis. Results CH + adalimumab group showed a significant decrease in peritoneal thickness, fibrosis score, and vascular score compared with CH group and CH + resting group. Conclusion Adalimumab can prevent SEP development.Canakkale Onsekiz Mart University Scientific Research Projects UnitCanakkale Onsekiz Mart University [TTU-2016-840]The study was supported by Canakkale Onsekiz Mart University Scientific Research Projects Unit (TTU-2016-840)
Impact of fingolimod on CD4+T cell subset and cytokine profile of relapsing remitting multiple sclerosis patients
No full textTurkoglu, Recai/0000-0001-9724-851X; Adin-Cinar, Suzan/0000-0002-8330-7010; Gunduz, Tuncay/0000-0003-4241-0908WOS: 000498318800020PubMed: 31526917Fingolimod inhibits the egress of lymphocytes from lymphatic tissues and also directly affects their functions by modulation of the sphingosine-1 -phosphate receptor 1 (S1P1). Our aim was to evaluate the impact of fingolimod on diverse CD4+ T cell subsets, and cytokines. Sixty-six relapsing remitting multiple sclerosis (RAMS) patients were treated with oral fingolimod (0.5 mg) for 6 months, and blood samples were collected at baseline, 3 months, and 6 months. Serum levels of seven cytokines and five chemokines were measured by multiplex immunoassay, and frequencies of peripheral blood mononuclear cell subsets were assessed by flow cytometry, and compared with those of 60 healthy controls. CCL2 (p = 0.039), and CCL5 (p = 0.001) levels were significantly higher in fingolimod-treated patients than healthy controls, whereas end-of-study serum levels of IL-6, IL-8, IL-17A, IL-22, IL-23, TNF-alpha, CXCL10, and CXCL13 were comparable to the baseline levels. Six months of fingolimod treatment reduced CD3+ T cell (mean +/- standard deviation, 72.9% +/- 5.5 vs. 60.1% +/- 11.1, p < 0.001), CD4+ T cell (62.2% +/- 8.5 vs. 24.6% +/- 12.9, p < 0.001), CD4+CD25hi regulatory T cell (Treg) (3.4% +/- 1.3 vs. 2.0% +/- 1.4, p < 0.01), and CD19+ B cell (13.2% +/- 5.8 vs. 5.3% +/- 2.7, p < 0.001) frequencies, while CD8+ T cells (31.8% +/- 7.8 vs. 57.8% +/- 13.2, p < 0.001) were increased, and NK and NKT cells remained unchanged. the proportions of intracytoplasmic IL-4, IL-10, IFN-gamma, and TNF-alpha-producing T cells were increased, whereas IL-17-producing cells remained relatively constant as measured by flow cytometry. Fingolimod appears to primarily diminish lymphocyte subsets involved in antigen presentation (CD19+ B and CD4+ T cells) rather than immune cells (CD8+ T, NK, and NKT cells) in charge of host defense against pathogens. in contrast, a relative increase is observed in pro- and anti-inflammatory cytokine-producing T helper subsets (IFN-gamma, TNF-alpha, IL-4, and IL-10-producing CD4+ T cells), suggesting that effector T cells are suppressed to a lesser degree by S1P1 modulation.Novartis PharmaceuticalsNovartisThis study was sponsored by Novartis Pharmaceuticals
A Rare Complication of Chronic Constipation: Lipoid Pneumonia
No full textLipoid pnömoni nadir görülür. Özellikle yaşlılarda kronik konstipasyon amacı ile kullanılan mineral yağlarda aspirasyon riski vardır. Burada zeytinyağı aspirasyonu sonucu gelişen lipoid pnömoni olgusu anlatılmaktadır.Yetmiş yaşında erkek hasta iki aydır devam eden öksürük, balgam şikayetleri ile başvurdu.Toraks BT’de sağ akciğer alt lob posterior segmentte ve sağ orta lobda yaygın buzlu cam dansiteleri saptandı.Nonspesifik antibiyoterapiden bir ay sonra çekilen Toraks BT’de orta lobda bulguların sebat ettiği görüldü. Sağ orta lobdan bronkoalveolar lavaj yapıldı. ARB, nonspesifik kültür, mantar ve sitoloji incelemeleri tanıya ulaştırmadı. Yeni toraks BT’de sağ orta lobda volüm kaybının geliştiği görüldü. Tanı ve tedavi amaçlı sağ orta lobektomi yapıldı. Patolojik incelemesi lipoid pnömoni ile uyumlu bulundu. Tekrar sorgulandığında kronik konstipasyonu için hergün sekiz yemek kaşığı zeytinyağı içtiği öğrenildi. Buzlu cam infiltrasyonu olan olgularda lipoid pnömoni akılda bulundurulmalıdır. Özellikle çocuklarda, yaşlılarda, reflü, yutma güçlüğü veya nörolojik hastalığı olan kişilerde laksatif amaçlı mineral yağ kullanımı bıraktırılmalıdır.Lipoid pneumonia is a rare lung disease. Consuming mineral oils for the specific treatment of chronic constipation can lead to aspiration. Herein, a case of lipoid pneumonia is presented resulting from the consumption of olive oil to treat constipation. A 70- year-old male referred with a cough and sputum expectoration. A patchy ground glass opacity was noted in right lower lobe posterobasal segment and the middle lobe in a thoracic computerized tomography (CT). The findings persisted in the middle lobe after broad spectrum antibiotherapy. An examination of the bronchoalveolar lavage fluid was inconclusive. A loss of volume was noted in the middle lobe in a new CT. A middle lobectomy was carried out, and the pathological examination revealed lipoid pneumonia. Under deep questioning, the patient disclosed that he consumed olive oil every morning for chronic constipation. Lipoid pneumonia can be an alternative diagnosis for ground glass opacities. Consuming mineral oil should be avoided, especially in the elderly and children, and in patients with impaired swallowing, reflux or neurological disorders
PERCUTANEOUS TREATMENT OF BILE LEAKAGE AFTER LIVING DONOR LIVER TRANSPLANTATION
No full textAmaç: Biliyer kaçak (BK), canlı donör karaciğer nakli (CDKN) sonrası sık görülen komplikasyonlar arasındadır. Radyolojik yöntemler, tanıda ve tedavide önemlidir. Endoskopik yaklaşımlar BK’larda ilk basamak tedavi seçeneği kabul edilmekle birlikte günümüzde artan deneyim girişimsel radyolojik yaklaşımların rolunü de giderek arttırmaktadır. Çalışmamızda, CDKN sonrası gelişen BK’ların tanı ve tedavisinde deneyimimizi analiz etmeyi amaçlıyoruz. Gereç ve Yöntemler: Ocak 2015 – Aralık 2018 arasında merkezimizde gerçekleştirilen ardışık 361 CDKT olgu retrospektif olarak analiz edildi. BK şüphesi bulunan hastalarda kaçak tanısı ve yeri, safra yollarından ekskrete edilen gadoksetate disodyum kontrast madde kullanarak alınan manyetik rezonans kolanjiyopankreatografi (MRKP) ile gösterildi. Safra kaçakları, endoskopik, girişimsel radyolojik yaklaşımlar, cerrahi ve konservatif olarak tedavi edildi. Bulgular: Total 361 olgunun 27’sinde (%7,4) BK mevcut idi. BK’ların 26’sı (%96,2) anastomotik, 1’i ise (%3,7) kesi yüzey kaçağı idi. Bir hasta (%3,7) endoskopik, 23 hasta (% 85,1) girişimsel radyolojik, iki hasta (%7,4) cerrahi ve bir hasta (%3,7) konservatif yaklaşım ile tedavi edildi. Girişimsel radyolojik işlemler sırasında herhangi minor ve major komplikasyon gelişmedi. İşlem sonrasında ise 6 hastada (%26) minor komplikasyon [4 (%17,4) hafif kolanjitik atak, 2 (%8,6) hafif ödematöz pankreatit] gelişti. Sonuç: CDKT sonrası safra yollarının çoklu anastomozlu olması endoskopik tedavi yaklaşımlarını zorlaştırmaktadır. Girişimsel radyolojik yaklaşımlar deneyim gerektirmekle birlikte alternatif yöntem olarak uygulanabilir.Objective: Biliary leakage (BL) is one of the most frequent complications following living donor liver transplantation (LDLT). Radiological methods are important in diagnosis and treatment. Although endoscopic approaches are considered as the firstline treatment option in BL, nowadays, increasing experience is strengthening the role of interventional radiological approaches. In our article, we aim to analyze our experience in the diagnosis and treatment of BLs developed after LDLT. Material and methods: Between January 2015 and December 2018, 361 LDLT cases performed consecutive at our center were analyzed retrospectively. The leakage diagnosis and location was demonstrated by magnetic resonance cholangiopancreatography (MRCP) with hepatobiliary excreted contrast agent gadoxetate disodium in patients with suspected leakage. BLs were treated by endoscopic, interventional radiological, surgical or conservative approaches. Results: Of the total 361 cases, 27 (7.4%) had BL. Twenty six (96%) of the BL were anastomotic and 1 (3,7%) was incision surface leakage. One patient (3.7%) was treated by endoscopic sphincterotomy methods, 23 (85,1%) patients by interventional radiological approaches, two patients (7.4%) by surgery and 1 patient (3,7%) by conservative approaches. There were no minor and major complications during interventional radiological procedures. After the procedure, minor complications (mild cholangitic attack) in 4 (17.4%), mild edematous pancreatitis in 2 (8,6%) developed in 6 (26%) patients. Conclusion: Endoscopic treatment approaches may be complicated in patients with multiple anastomosis of biliary tract after LDLT. Although interventional radiological approaches require experience, they can be performed as an alternative treatment method
EFFECTS OF FLUORESCENT MARKED MAACKIA AMURENSIS-LECTIN-1 AND WHEAT GERM AGLUTIN ON THE CELL SURFACE GLYCAN PROFILES IN TWO DIFFERENT BREAST CANCER CELL LINES
No full textAmaç: Meme kanseri teşhisinde patolojik tetkikler gibi birçok yöntem kullanılmaktadır. Tüm bu tetkiklerin ve yöntemlerin yanı sıra, kanser araştırmalarında glikobiyolojinin de önemi oldukça artmıştır. ER (+) özellik gösteren MCF-7 ve ER (-) özellik gösteren MDAMB-231 meme kanseri hücre hatlarının zar yüzeylerinde görülen şeker rezidüleri miktar farklılıkları FITC-(Maackia Amurensis-Lektin-1) Ve FITC-(Wheat Germ Aglutin) ile spesifik olarak işaretlenmiş ve ışıma yoğunluğu göreceli olarak irdelenmiştir. Bu yöntem ile meme kanseri hücre soylarının membranlarında bulunan sialik asit birimlerindeki farklılıkları iki saat gibi kısa bir zamanda, hassas olarak ayrılabildiğinin açıkça ortaya çıkarılması amaçlanmıştır. Gereç ve Yöntem: Çalışmamızda MCF-7 ve MDA-MB-231 hücre soyları kullanılmıştır. Çalışmada kullanılması planlanan floresan işaretli lektinler [FITC-(Maackia Amurensis-Lektin-1) Ve FITC-(Wheat Germ Aglutin)] ilgili firmalardan satın alınmış ve kullanım protokolüne uygun olarak hazırlanıp işaretlemeler yapılmıştır. Bulgular: MCF-7 ve MDA-MB-231 meme kanseri hücre hatlarına FITC işaretli Maackia amurensis lektini-1 uygulanmıştır. Buna göre; MDA-MB-231 hücre hattı MCF-7’a göre göreceli olarak daha yoğun bir ışıma göstermiştir. Aynı kanser hücre hatlarına FITC işaretli Wheat Germ Aglutinin uygulanmıştır. Bu işaretleme sonucunda da her iki kanser hattında da benzer yoğunlukta ışıma tespit edilmiştir. Sonuç: Çalışmada kullanılan MCF-7 ve MDA-MB-231 hücre hat arının membranların da görülen bu farklılıklar önemli bulunmuştur. Hücrelere uygulanan iki saatlik boyama yöntemi ile iki farklı meme kanseri hücre tipi hızlı bir şekilde ayrılmıştır. Aynı zamanda immmünofloresan işaretli lektinler yardımıyla, glikokonjugatların, hızlı ve özgün ayrımda kullanılabilirliği gösterilmiştir.Objective: Many methods such as pathological examinations are used to diagnose breast cancer. In addition to all these tests and methods, the use of glycobiology has also increased significantly in cancer research. Differences in sugar residues observed on the membrane surfaces of breast cancer cell lines ER (+) -creating MCF-7 and ER (-) MDA-MB-231 were specifically marked with FITC-(Maackia Amurensis-Lectin-1) Ve FITC-(Wheat Germ Aglutinin) The intensity of radiation is discussed relatively. The aim of using this method is to clearly reveal that the differences in the sialic acid units in the membranes of breast cancer cell lines can be precisely separated in as little as two hours. Material and Method: MCF-7 and MDA-MB-231 ) cells were used in our study. [FITC-(Maackia Amurensis-Lectin-1) Ve FITC- (Wheat Germ Aglutin)] were purchased from the respective companies. Following this step, the FITC marked products were prepared and marked according to usage protocols. Results: FITC-labeled Maackia amurensis Lectin-1 was applied to MCF-7 and MDA-MB-231 breast cancer cell lines. Subsequently, the MDA-MB-231 cell line showed a relatively more intense radiation than the MCF-7. The FITC marked Wheat germ agglutinin was applied to the same cancer cell lines. As a result of this marking, radiation of similar intensity was detected in both cancer lines. Conclusion: These differences seen in the membranes of the MCF-7 and MDA-MB-231 cell lines used in the study are significant. Two different types of breast cancer cell types were rapidly separated using the two hour staining method applied to the cells. At the same time, with the help of immunofluorescent-labeled lectins, the availability of glyconoconjugates was demonstrated for rapid and specific discrimination
Comparison of the Effect of Phytohemagglutinin and Interleukin-2 on Human-T-Cells
No full textAmaç: Bu çalışmada, CD3+T-hücrelerini fitohemaglutinin (phytohemagglutinin,PHA), İnterlökin-2(interleukin-2,IL-2) ve PHA+IL-2 ile birlikte uyararak, farklı uyarıcılarla uyarılmanın CD3+T-hücre çoğalım ve aktivasyon molekülleri ekspresyonuna etkisinin belirlenmesi amaçlandı. Gereç ve Yöntem: Sağlıklı 15 kadının herbirinden 6 mL periferik kan örneği alınarak RosetteSep yöntemiyle 3x106 adet CD3+T-hücre izole edildi. CD3+T-hücreleri faz-kontrast mikroskopta sayıldı ve izolasyon saflıkları immunfloresan ve akım sitometrik yöntemlerle belirlendi. 12 kuyucuklu kültür kabının 3’er kuyucuğuna 2x105 adet paylaştırılan hücrelere 10 ?g/mL PHA, 13?L/mL IL-2 ve 10 ?g/mL PHA+13?L/mL IL-2 uygulanarak 24 saat inkübatörde inkübe edildi. Uyarıcıyla uyarılmamış CD3+T-hücreleriyle (kontrol grubu) karşılaştırılan uyarılmış CD3+T–hücre gruplarının; çoğalımları spektrofotometrik yöntemle WST-1 testi ve akım sitometride CFSE testi ile aktivasyon moleküllerinin ekspresyonları CD25,CD38,CD69,CD71 ve HLA-DR antikorları ile akım sitometride ölçüldü. Bulgular: WST-1 ve CFSE test değerleri gruplar arasında istatistiksel olarak karşılaştırıldığında; PHA-CD3+T-hücrelerinin (p <0.001) ve PHA+IL- 2-CD3+T-hücrelerinin (p <0.001) IL-2-CD3+T-hücrelerinden anlamlı olarak daha fazla çoğaldıkları yani uyarıldıkları tespit edildi. Akım sitometride ölçülen aktivasyon moleküllerinin düzeyleri gruplar arasında istatistiksel olarak karşılaştırıldığında; IL-2-CD3+T-hücrelerine kıyasla, PHA-CD3+Thücrelerinde ve PHA+IL-2-CD3+T-hücrelerinde CD25 (p <0.001), CD38 (p <0.001), CD71 (p <0.001) ve HLA-DR (p <0.001) % ekspresyon düzeylerinde anlamlı artış tespit edildi. Sonuç: PHA ve PHA+IL-2 ile uyarılan CD3+T-hücrelerinde hücre proliferasyonunun ve aktivasyon molekülleri düzeylerdinin benzer olduğu ve IL-2 ile uyarılan hücrelere kıyasla fazla olduğu tespit edildiğinden, invitro çalışmalarda CD3+T-hücre uyarıcısı olarak PHA kullanılmasının başka bir uyarıcıya gerek duyulmaksızın tek başına etkin olduğu ve CD3+T-hücrelerde IL-2’ye kıyasla daha fazla çoğalımı ve uyarılmayı sağladığı sonucuna varıldı.Objective: In this study, it is aimed to determine the effect of stimulation with different stimuli on CD3+T-cells proliferation and activation molecules by stimulating phytohemagglutinin (PHA), Interleukin-2 (IL-2) and PHA+IL-2 together. Material and Method: Six mL peripheral blood samples were obtained from each 15 healthy women and their 3x106 CD3+T-cells were isolated by the RosetteSep method. CD3+T-cells were counted on a phase-contrast microscope and their isolation purity were determined by immunofluorescence and flow cytometric methods. 2x105 cells were apportioned in a 3-well dish of a 12-well culture dish, applied 10?g/mL PHA, 13?L/mL IL-2 and 10?g/mL PHA+13 ?L/mL IL-2 and incubated in incubator for 24 hours. Cells groups of stimulated CD3+T-cells compared with CD3+T-cells(control group) not stimulated with any stimulants; their proliferations measured by spectrophotometric method using WST-1 test and flow cytometry by CFSE test, their expressions of activation molecules measured by flow cytometry with CD25, CD38, CD69, CD71 and HLA-DR antibodies. Results: When WST-1 and CFSE test values were compared statistically between groups; It was determined that PHA-CD3+T-cells (p <0.001) and PHA + IL-2-CD3+T-cells (p <0.001) were significantly more proliferated, in other words activated than IL-2-CD3+T-cells. When levels of activation molecules evaluated by flow cytometry were compared statistically between groups; in comparison to IL-2-CD3+T-cells, significantly increased % expression values of CD25 (p <0.001), CD38 (p <0.001), CD71 (p <0.001) and HLA-DR (p <0.001) were detected in PHA-CD3+T-cells and PHA+IL-2-CD3+T-cells. Conclusion: Since the proliferation and activation molecule levels in CD3+T-cells stimulated by PHA and PHA + IL-2 were similar and more than than stimulated by IL-2, it was concluded that the use of PHA as a CD3+T-cell stimulant in invitro studies alone was effective with no need for other stimulants and provided more proliferation and stimulation compared to IL-2 in CD3+T-cells
Effects of boric-acid-applied green tea on the expressions of heat shock proteins in MCF-7 cells
No full textWOS: 000458742100009Purpose: The study aims to examine the effects of boric acid at different concentrations applied to green tea on heat shock protein (HSP) 70 and HSP 90 in MCF-7 cells. Materials and Methods: Boric acid in sodium tetraborate buffer of 0 mg/m(2) (group 1), 100 mg/m(2) (group 2), 300 mg/m(2) (group 3), and 500 mg/m(2) (group 4) were applied to soil where Camelia sinensis (green tea) grown. The collected green tea leaves were dried and brewed in water at 80 degrees C for 40 minutes. Cytotoxicity was analyzed by MTT method. The prepared green tea extracts (250 mu g/ml) were applied to MCF-7 cells. The mRNA expressions of HSP 70 and HSP 90 were determined by qRT-PCR. The HSP 70 and HSP 90 protein levels were measured by Western blotting method. Results: 100 and 500 mg/m(2) boric-acid-applied green tea extracts increased HSP 90 mRNA expressions in MCF-7 cells compared to green tea extract without boric acid. HSP 70 and HSP 90 protein levels did not show any change between MCF-7 cells with boric-acid-applied green tea extract and green tea extract without boric acid. Conclusion: According to our results, the protein expressions of HSP 70 and HSP 90 in MCF-7 cells changed by neither boric-acid-applied green tea extracts nor green tea extracts without any boric-acid-applied
The Impact of Peritonitis on Clinical Outcomes of PD Patients: A Single Center Experience
No full textWOS: 000461206800012Objective: The aim of the present study was to investigate peritonitis episodes and causative agents in patients undergoing peritoneal dialysis (PD), to examine the effects of peritonitis on technical and patient survival, and to determine whether the number of peritonitis episodes and causative agents was a risk factor or not. Materials and Methods: The medical records of 387 patients who started PD between January 2001 and January 2015 were evaluated retrospectively. Patients without peritonitis (Group 1 (n=123 patients)) and with detected peritonitis (Group 2 (n=243 patients)) were divided into two groups. Group 2 patients were subdivided according to the number of peritonitis (Group 2a 1 episode and Group 2b >= 2 episodes). Sociodemographic data and clinical courses were compared, and the reasons for PD withdrawal were obtained between the groups. Survival analysis was performed, and the effects of peritonitis on mortality were investigated. Results: A total of 427 peritonitis episodes were detected. The most common organism was Staphylococcus aureus (36%). The leading cause of death was cardiovascular disease in Group 1, whereas it was infection in Group 2a and Group 2b. Technique survival and mortality rates were similar among the groups. Risk factors for patient survival were history of peritonitis more than once and history of catheter exit site/tunnel infection. History of catheter exit site/tunnel infection was the only risk factor for technique survival. Conclusion: Our study has shown that even though the causes for mortality were different, mortality rates, and technique survival were similar between the two main groups. Infectious complications may affect patient and technical survival
The effects of diffusion and perfusion MRI mismatch on the admission blood glucose and blood pressure values measured in the first 12 hours of acute stroke
No full textPubMed ID: 31261574Patients with severe stroke and salvageable brain tissue at admission, who have higher glycaemic and blood pressure levels, may have a risk of iatrogenic hypoglycemia/iatrogenic hypotension. In this study, we examined the relationship between the presence of diffusion-weighted imaging (DWI)/perfusion-weighted imaging (PWI) mismatch, admission blood glucose level, and admission blood pressure level in patients who were admitted in the first 12 hours after onset. We studied 212 patients who were prospectively and consecutively registered to the stroke unit from 2006 to 2009. Correlations between mismatch and admission blood pressure level (ABPL) and admission blood glucose level (ABGL) were analyzed using multivariate logistic regression. Mismatch (P?=?.064, adjusted OR?=?2.297, 95% CI, 0.953-5.536) was not associated with a high ABGL in the whole group. However, after excluding patients with diabetes mellitus (DM) (n?=?67, 35%), mismatch (P?=?.033, adjusted OR?=?3.801, 95% CI, 1.110-13.015), an impaired level of consciousness, use of anti-DM medication, glycated hemoglobin levels, and cardioembolic aetiology were independent predictors of a high ABGL. The presence of mismatch or proximal vessel occlusion was not associated with ABPL. Female sex (P?=?.048) and total anterior circulation stroke (P?=?.008) were independent predictors associated with a higher ABPL. We conclude that patients with hyperacute ischemic stroke with PWI/DWI mismatch are more likely to have hyperglycemia
Body Mass Index and Unenhanced CT as a Predictor of Hepatic Steatosis in Potential Liver Donors
No full textBackground: In living-donor liver transplantation, donor hepatic steatosis is crucial for both the donor and the recipient. Body mass index (BMI) and the unenhanced computed tomography liver attenuation index (CT LAI) are noninvasive methods to predict hepatic steatosis in living-donor liver candidates. Aim: To analyze the diagnostic accuracy of CT LAI in conjunction with different BMI values for macrovesicular steatosis in living-donor liver candidates. Methods: A total of 264 potential liver donors were included. The diagnostic accuracy of 2 CT LAI cut-offs and 3 BMI cut-off values for the assessment of hepatic steatosis ?15% and ?5% was determined. Results: Using CT LAI, the area under the receiver operating characteristic curve was 0.97 (95% CI = 0.89–0.99) for hepatic steatosis ?15% in donors with BMI <25 kg/m2. For detecting hepatic steatosis ?15%, a CT LAI ?0 had specificities of 100%, 76.2%, and 55.6% and positive predictive values of 100%, 95.5%, and 93.5% for patients with BMI values <25 kg/m2, 25 to 29.9 kg/m2, and ?30 kg/m2, respectively. According to logistic regression analyses, only CT LAI ?0 was found to be independently associated with hepatic steatosis ?15%. Conclusions: In donors with BMI <30 kg/m2 and a CT LAI value >6, liver biopsy might be avoided. Biopsy may be reserved solely for donors with CT LAI value >0 and BMI ?30 kg/m2 as the diagnostic accuracy of computed tomography for predicting hepatic steatosis decreases with increasing BMI. © 2019 Elsevier Inc