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Phocoena phocoena (harbor porpoise) - CCSN-03-023
Phocoena phocoena (harbor porpoise) - CCSN-03-023 - female 1.2 m - Pelvic location - Museum of Osteology, Oklahoma City, OK
Phocoena phocoena (harbor porpoise) - MCZ-51697
Phocoena phocoena (harbor porpoise) - MCZ-51697 - unknown sex - unknown length - Pelvic location - Harvard Universit
Physeter catodon (sperm whale) - UF-30916
Physeter catodon (sperm whale) - UF-30916 - female - 10.7 m - University of Florid
Microbial functional diversity across biogeochemical provinces in the central Pacific Ocean
© The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Saunders, J. K., McIlvin, M. R., Dupont, C. L., Kaul, D., Moran, D. M., Horner, T., Laperriere, S. M., Webb, E. A., Bosak, T., Santoro, A. E., & Saito, M. A. Microbial functional diversity across biogeochemical provinces in the central Pacific Ocean. Proceedings of the National Academy of Sciences of the United States of America, 119(37),(2022): e2200014119, https://doi.org/10.1073/pnas.2200014119.Enzymes catalyze key reactions within Earth’s life-sustaining biogeochemical cycles. Here, we use metaproteomics to examine the enzymatic capabilities of the microbial community (0.2 to 3 µm) along a 5,000-km-long, 1-km-deep transect in the central Pacific Ocean. Eighty-five percent of total protein abundance was of bacterial origin, with Archaea contributing 1.6%. Over 2,000 functional KEGG Ontology (KO) groups were identified, yet only 25 KO groups contributed over half of the protein abundance, simultaneously indicating abundant key functions and a long tail of diverse functions. Vertical attenuation of individual proteins displayed stratification of nutrient transport, carbon utilization, and environmental stress. The microbial community also varied along horizontal scales, shaped by environmental features specific to the oligotrophic North Pacific Subtropical Gyre, the oxygen-depleted Eastern Tropical North Pacific, and nutrient-rich equatorial upwelling. Some of the most abundant proteins were associated with nitrification and C1 metabolisms, with observed interactions between these pathways. The oxidoreductases nitrite oxidoreductase (NxrAB), nitrite reductase (NirK), ammonia monooxygenase (AmoABC), manganese oxidase (MnxG), formate dehydrogenase (FdoGH and FDH), and carbon monoxide dehydrogenase (CoxLM) displayed distributions indicative of biogeochemical status such as oxidative or nutritional stress, with the potential to be more sensitive than chemical sensors. Enzymes that mediate transformations of atmospheric gases like CO, CO2, NO, methanethiol, and methylamines were most abundant in the upwelling region. We identified hot spots of biochemical transformation in the central Pacific Ocean, highlighted previously understudied metabolic pathways in the environment, and provided rich empirical data for biogeochemical models critical for forecasting ecosystem response to climate change.Funding for this research was provided by the Gordon and Betty Moore Foundation (grants 3782 and 8453), the US NSF (NSF grants OCE-1924554, 2123055, 2125063, 2048774, and 2026933), the Center for Chemical Currencies on a Microbial Planet (NSF grant OCE-2019589), and the US NIH General Medicine (grant GM135709-01A1). J.K.S. was supported by a NASA Postdoctoral Program Fellowship with the NASA Astrobiology Program, administered by Universities Space Research Association under contract with NASA. A.E.S. was supported by the Sloan Foundation, the Simons Foundation, and NSF grant OCE-1437310. A portion of this research used resources at the US Department of Energy JGI sponsored by the Office of Biological and Environmental Research and operated under contract DE-AC02-05CH11231 (JGI). C.L.D. and D.K. were supported by NSF grants OCE-1558453 and OCE-2049299. T.H. was supported by NSF grant OCE-2023456
Benthic invertebrate taxonomy, species and codes - St. John USVI survey 1988-2007 (St. John LTREB project)
Dataset: benthic invert codesFor a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/523731NSF Division of Environmental Biology (NSF DEB) DEB-0841441, NSF Division of Environmental Biology (NSF DEB) DEB-034357
Benthic invertebrates from 6 sites pooled, USVI: 1992-2007 (St. John LTREB project)
Dataset: inverts - pooledBenthic invertebrates from 6 sites pooled, USVI: 1992-2007.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/525499NSF Division of Environmental Biology (NSF DEB) DEB-0841441, NSF Division of Environmental Biology (NSF DEB) DEB-034357
Supplemental materials for book chapter: Microplastics in Marine Food Webs
The identification of microplastics (MPs; 1 µm - 5 mm) and the inferred presence of nanoplastics (NPs; <1 µm) in a wide variety of marine animals, including many seafood species, has raised important questions about the presence, movement, and impacts of these particles in marine food webs. Understanding microplastic dynamics in marine food webs requires elucidation of the processes involved, including bioaccumulation, trophic transfer, and biomagnification. However, in the context of microplastics and nanoplastics these concepts are often misunderstood. In this chapter, we provide a critical review of the literature on the behavior of plastic particles in marine food webs. We find clear evidence of trophic transfer, equivocal evidence for bioaccumulation, and no evidence for biomagnification. We also identify a number of knowledge gaps that limit our ability to draw firm conclusions at this time.
These supplemental documents are in support of an invited chapter to be published in this book: S.E. Shumway and J.E. Ward (Eds.) Plastics in the Sea: Occurrence and Impacts (Elsevier 2023).Preparation of this chapter was supported by Woods Hole Sea Grant (Award No. NA18OAR4170104, project R/P–89), a NSF Graduate Research Fellowship to J.A.P.; the Gerstner Family Foundation; and the March Marine Initiative, a program of March Limited, Bermuda
Seagrass metrics from from seagrass wasting disease mesocosm experiments conducted at Bodega Marine Laboratory from July-September 2015
Dataset: Mesocosm warming experiment seagrass metricsThis dataset includes seagrass metrics (diversity, biomass, growth, and wasting disease) from mesocosm experiments at Bodega Marine Laboratory in July-September 2015. Data were collected as part of a mesocosm study at the Bodega Marine Laboratory examining the independent and interactive effects of warming, host genotypic identity, and host genotypic diversity on the prevalence and intensity of infections of seagrass by the wasting disease parasite Labyrinthula zosterae.
These data were published in Schenck et al (2022).
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/879749NSF Division of Ocean Sciences (NSF OCE) OCE-165232
Microbial taxa (amplicon sequence variant or ASV) statistical analyses for two seagrass genotypes from wasting disease mesocosm experiments at Bodega Marine Laboratory in July-Sept of 2015
Dataset: Mesocosm microbial analyses resultsThis dataset includes outputs from statistical analyses of differences in microbial taxa (amplicon sequence variant or ASV) abundance among two groups of seagrass, Zostera marina, genotypes: those that showed reduced Labyrinthula zosterae parasites when warmed vs those that showed increased L. zosterae parasites when warmed; and two seawater temperature treatments: ambient or elevated +3.2oC. Data were collected as part of a mesocosm study at the Bodega Marine Laboratory examining the independent and interactive effects of warming, host genotypic identity, and host genotypic diversity on the prevalence and intensity of infections of seagrass by the wasting disease parasite L. zosterae.
These data were published in Schenck et al (2022). Related sequence data from this experiment is accessible from the National Center for Biotechnology Information (NCBI) BioProject PRJNA716355.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/883070NSF Division of Ocean Sciences (NSF OCE) OCE-165232
Fish and invertebrate transect survey dataset from the West coast of Leyte, the Philippines in the municipalities of Albuera (10.91667, 124.69667) and Bay Bay City (11.07611, 124.87525), 2014-2017
Dataset: Fish and Invertebrate TransectsFish and invertebrate transect survey dataset from the West coast of Leyte, the Philippines in the municipalities of Albuera (10.91667, 124.69667) and Bay Bay City (11.07611, 124.87525), 2014-2017.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/862405NSF Division of Ocean Sciences (NSF OCE) OCE-143021