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Seawater concentration data from an ocean acidification exposure experiment on adult Eastern oysters from Plum Island Sound in 2017
Full text linkDataset: Seawater and Extrapallial fluid ConcentrationTrace, minor, and major element data from adult Eastern oyster ocean acidification exposure experiments were conducted at the Ries Lab at the Northeastern University Marine Science Center on samples from Plum Island Sound in 2017. This dataset represents the phenotypic and molecular responses in the extrapallial fluid in the adult eastern oyster (Crassostrea virginica) exposed to experimental ocean acidification (OA) over 80 days.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/888887NSF Division of Ocean Sciences (NSF OCE) OCE-163542
Trapping of nicotinic acetylcholine receptor ligands assayed by in vitro cellular studies and in vivo PET imaging
No full textAuthor Posting. © Society for Neuroscience, 2023. This article is posted here by permission of Society for Neuroscience for personal use, not for redistribution. The definitive version was published in The Journal of Neuroscience 43(1), (2023): 2-13, https://doi.org/10.1523/JNEUROSCI.2484-21.2022.A question relevant to nicotine addiction is how nicotine and other nicotinic receptor membrane-permeant ligands, such as the anti-smoking drug varenicline (Chantix), distribute in brain. Ligands, like varenicline, with high pKa and high affinity for α4β2-type nicotinic receptors (α4β2Rs) are trapped in intracellular acidic vesicles containing α4β2Rs in vitro. Nicotine, with lower pKa and α4β2R affinity, is not trapped. Here, we extend our results by imaging nicotinic PET ligands in vivo in male and female mouse brain and identifying the trapping brain organelle in vitro as Golgi satellites (GSats). Two PET 18F-labeled imaging ligands were chosen: [18F]2-FA85380 (2-FA) with varenicline-like pKa and affinity and [18F]Nifene with nicotine-like pKa and affinity. [18F]2-FA PET-imaging kinetics were very slow consistent with 2-FA trapping in α4β2R-containing GSats. In contrast, [18F]Nifene kinetics were rapid, consistent with its binding to α4β2Rs but no trapping. Specific [18F]2-FA and [18F]Nifene signals were eliminated in β2 subunit knock-out (KO) mice or by acute nicotine (AN) injections demonstrating binding to sites on β2-containing receptors. Chloroquine (CQ), which dissipates GSat pH gradients, reduced [18F]2-FA distributions while having little effect on [18F]Nifene distributions in vivo consistent with only [18F]2-FA trapping in GSats. These results are further supported by in vitro findings where dissipation of GSat pH gradients blocks 2-FA trapping in GSats without affecting Nifene. By combining in vitro and in vivo imaging, we mapped both the brain-wide and subcellular distributions of weak-base nicotinic receptor ligands. We conclude that ligands, such as varenicline, are trapped in neurons in α4β2R-containing GSats, which results in very slow release long after nicotine is gone after smoking.This work was supported in part by National Institutes of Health (NIH) Grants R01 DA044760-01 (to J.M., C.-T.C. and W.N.G.), RF1 AG029479 (to J.M.), and T32 DA043469 (to M.Z.). The authors acknowledge the assistance from the Integrative Small Animal Imaging Research Resources (iSAIRR) supported in part by NIH Grants P30 CA14500 and S10 OD025265 and from the Cyclotron Facility of the University of Chicago.2023-07-0
Sponge Density, Morphology, and Assemblages from repeated surveys in St. Thomas, U.S. Virgin Islands, before and after the 2017 hurricane season
No full textDataset: Sponge Density, Morphology, and AssemblagesPrior to the 2017 hurricanes, six shallow (8-15 meter depth) reef sites had been selected from the Virgin Islands Territorial Coral Reef Monitoring Program’s (TCRMP) permanent monitoring sites to study variation in sponge communities- Black Point (BP), Coculus Rock (CR), and Magens Bay (MB), which are in embayments with heavily developed watersheds. Buck Island (BI) and Savana Island (SI) are located near undeveloped offshore cays. Botany Bay (BB) is a nearshore site in a bay with a low level of watershed development.
This dataset represents sponge density, morphology, and assemblages from these repeated surveys before and after the 2017 hurricane season. We used three randomly selected transects out of the six permanently established 10-meter TCRMP transects at each site. The same three transects at each site were re-surveyed repeatedly in August 2016 (pre-hurricanes), December 2017 (10 weeks post-hurricanes), March 2018 (24 weeks post-hurricanes), November 2018 (61 weeks post-hurricanes), and July 2019 (93 weeks post-hurricanes).
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/890333NSF Division of Ocean Sciences (NSF OCE) OCE-18078072024-01-0
Optimally Interpolated O2 anomalies based on World Ocean Database 2018
Full text linkDataset: OIO2-WOD2018OIO2 is a gridded data product of dissolved oxygen interpolated from shipboard observations archived in the World Ocean Database 2018 (WOD18). The quality-controlled WOD18 data are averaged for each bin at 1°x1° and monthly resolution where mean, variance, and sample size are recorded from 1965 to 2014 for the bottle data, and from 1987 to 2014 for the CTD-O2 data.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/886218NSF Division of Ocean Sciences (NSF OCE) OCE-212354
Intracellular pH (pHi) data collected as part of a study of pCO2 variability on the reef-building coral Pocillopora damicornis conducted at Heron Island Research Station, Heron Island, southern Great Barrier Reef in 2021
No full textDataset: Growth and physiology of Pocillopora damicornis: pHiThis dataset contains intracellular pH (pHi) data. These data were published in Brown et al. (2022).
Abstract for all data from the study (Brown et al., 2022) including this dataset:
Ocean acidification is a growing threat to coral growth and the accretion of coral reef ecosystems. Corals inhabiting environments that already endure extreme diel pCO2 fluctuations, however, may represent acidification resilient populations capable of persisting on future reefs. Here, we examined the impact of pCO2 variability on the reef-building coral Pocillopora damicornis originating from reefs with contrasting environmental histories (variable reef flat vs. stable reef slope) following reciprocal exposure to stable (218 ± 9) or variable (911 ± 31) diel pCO2 amplitude (μtam) in aquaria over eight weeks. This study measured: growth (net calcification, extension, CaCO3 density) and physiology (dark respiration, light-enhanced dark respiration, host soluble protein, mycosporine-like amino acids, net photosynthesis, photosynthetic efficiency, endosymbiont density, chlorophyll a concentration, intracellular pH) of P. damicornis across treatment and origin.
See all datasets related to this publication (https://www.bco-dmo.org/related-resource/885684).
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/885646NSF Division of Ocean Sciences (NSF OCE) OCE-192374
Marine parasites in island-like disturbed habitats
Full text linkSubmitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2023.Parasites are taxonomically and functionally diverse members of biological communities, and can play key roles in species interactions, community structure, and ecosystem functioning. For their reliance on host species, parasites are theorized to be particularly sensitive to disturbances that alter host diversity and abundance, especially in isolated habitats, which present
challenges to introduction and establishment. In this thesis, I investigate habitat isolation and disturbance as drivers of parasite diversity, with an emphasis on parasite life history strategies related to colonization and persistence. I focus on an island-like, frequently disturbed habitat, deep sea hydrothermal vents at 9°50’N on the East Pacific Rise, to explore the boundaries of
parasite persistence in an extreme environment. First, I analyze recovery in the vent community for 11 years after a catastrophic eruption in 2006 to test successional hypotheses in a new setting with distinct fauna and a chemosynthesis-based food web. Second, I compare parasite diversity at isolated, disturbed vents to marine ecosystems that are similarly isolated but undisturbed (atoll sandflat) and both well connected and undisturbed (kelp forest). Overall, parasite diversity within host species was not significantly lower at vents, but the vent community had many fewer parasite species because there are fewvertebrate predator species (fish). Parasites with indirect (multi-host) life cycles were relatively diverse in the disturbed environment, which contradicts expectation based on theory. To explore this further, I investigate the three-host life cycles of trematodes at vents, whichwas the most diverse and abundant parasite taxon. All life stages of the trematode life cyclewere discovered in vent fauna and several taxawere traced across multiple life stages via morphology and genetics. Finally, I use a computational model to investigate how different parasite strategies (colonization capability and impact on hosts) contribute to parasite success under a range of disturbance conditions in island habitats. Parasites that reduce host reproduction reached higher densities than parasites that cause mortality across all disturbance frequencies explored, and disturbance facilitated the evolution of more virulent parasites. These studies demonstrate that life history traits and the ability to adapt allow diverse parasite taxa to persist in isolated, ephemeral environments.I extend my thanks to the grantors and funding agencies who supported my research, including NSF grants OCE-1829773 and OCE-84773500, the Grassle Student Fellowship Fund, and the Ocean Ventures Fund
Mass transfer and chemical interactions in subduction zones
Full text linkSubmitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2023.Subduction zones are important sites of material recycling on Earth, with volatiles playing key roles in mass transfer processes and magma formation. This thesis investigates outstanding questions associated with a continuum of interrelated processes that occur as oceanic plates descend in subduction zones by integrating petrological and geochemical constraints from exhumed high-pressure rocks and erupted arc magmas, high pressure-temperature laboratory experiments, and thermodynamic calculations. Chapters 2 and 3 investigate the fluid-mediated reactions between mafic and ultramafic rocks at conditions relevant to the slab-mantle interface and show that Mg-metasomatism of mafic rocks to form chlorite-rich assemblages is favored and is likely more pervasive in subduction zones than in oceanic settings. Contrary to common belief, talc is unlikely to form in high abundance in ultramafic rocks metasomatized by Si-rich slabderived fluids. This means that talc-rich assemblages formed via Si-metasomatism along the slabmantle interface are less likely to be playing prominent roles in volatile transport, in facilitating slow-slip events, and in controlling the decoupling-coupling transition of the plate interface. Chapter 4 experimentally investigates the phase equilibria, melting, and density evolution of mélange rocks that formed by mixing and fluid-rock interactions. Results show that melting of
mélanges is unlikely to occur along slab-tops at pressures ≤ 2.5 GPa. Accordingly, diapirism into the hotter mantle wedge would be required to initiate melting. The density contrast between mélanges and the overlying mantle would allow for buoyancy-driven diapirism at relatively low pressures and melting could subsequently occur in the hotter mantle wedge during ascent.
However, diapir buoyancy may be limited at higher pressures due to the formation of abundant garnet especially in mélange rocks with peraluminous composition. Chapter 5 experimentally investigates the compositions of melts and mineral residues from melting of a mantle wedge hybridized with small amounts of mélange rocks to simulate an end-member scenario where solid
mélange diapirs dynamically interact with the mantle wedge. Results from laboratory experiments show that melting of a mélange-hybridized mantle wedge can produce melts that display compositional characteristics similar to arc magmas. Finally, Chapter 6 presents new interpretations on the evolution of slab-to-mantle transfer mechanisms from subduction initiation to arc maturity. Analyses of published magma compositions from global arcs reveal that melting of mélange plays an increasingly important role in magma formation as slab-tops cool and arcs mature over time. This trend is attributed to the deepening of the decoupled plate interface during subduction where mélange zones can form more extensively and contribute to the melting process more significantly. Taken together, this thesis highlights (i) the dynamic connection between mechanical mixing of different lithologies and fluid-rock interactions along the slab-mantle interface, (ii) how these processes modify the petrophysical and geochemical properties of subducted materials, and (iii) how these processes collectively influence the mechanisms of slabto-mantle transfer, elemental cycles, and the formation of arc magmas worldwide.This work has been supported financially by the National Science Foundation-Office of International Science & Engineering, Petrology & Geochemistry (NSF-OISE) PIRE, Award# 1545903 to F.K. and the Geoprisms program Collaborative Research Award# 1852610 to V.L.R. Research support through the WHOI Ocean Ventures Fund, US Science Support Program E-FIRE European Training Fund, WHOI Conference Fund are awarded to E.A.C
Physiology color score extracted from pictures taken during a heatwave experiment done September to November 2018 using reef building corals collected in Kāne'ohe Bay, O'ahu, Hawai'i.
No full textDataset: Heatwave Experiment: Color Score PhysiologyTwo common reef-building corals, Montipora capitata and Pocillopora acuta, were subjected to a extended heatwave scenario. Fragments were allowed to acclimate in experimental tanks for two weeks prior to exposure to one of the following four treatments: Ambient Temperature Ambient pCO2 (ATAC), Ambient Temperature High pCO2 (ATHC), High Temperature Ambient pCO2 (HTAC), and High Temperature High pCO2 (HTHC). The treatment period lasted for a two month period, starting on September 22nd, 2018 and lasting through November 17th, 2018. Following the stress period, coral fragments were exposed to a two-month recovery period in ambient conditions.
Throughout the entire four-month experiment, photos of the coral individuals were taken to extract a "color score". This dataset contains the processed data to calculate "color score" values.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/884208NSF Division of Ocean Sciences (NSF OCE) OCE-175662
Bottle chemistry data from hydrothermal vents sampled by CTD rosette during R/V Atlantis cruise AT37-12 in April and May 2017
Full text linkDataset: Bottle chemistry data from AT37-12The Eastern Tropical North Pacific (ETNP), like the other marine oxygen deficient zones (ODZs), is characterized by an anoxic water column, nitrite accumulation at the anoxic core, and fixed nitrogen loss via nitrite reduction to N2O and N2 gases. Here, we constrain the relative contribution of biogeochemical processes to observable features such as the secondary nitrite maximum (SNM) and local pH maximum by simultaneous measurement of inorganic nitrogen and carbon species. Bottle chemistry data for discrete depths were sampled by CTD rosette from hydrothermal vents in the Eastern Tropical North Pacific during R/V Atlantis cruise AT37-12 in April and May 2017.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/889539NSF Division of Ocean Sciences (NSF OCE) OCE-1559198, NSF Division of Ocean Sciences (NSF OCE) OCE-155904
Elemental and isotopic noble gas ratios from the Bermuda Atlantic Time-series (BATS) on cruise 10391 on R/V Atlantic Explorer (AE2208) from 30 April 2022 to 05 May 2022
Full text linkDataset: Elemental and isotopic noble gas ratios and N2/Ar from the North Atlantic: BATS dataThis dataset includes new observations of heavy noble gas ratios (elemental and isotopic ratios) from the Bermuda Atlantic Time-series (BATS) on cruise 10391 on R/V Atlantic Explorer (AE2208) from 30 April 2022 - 05 May 2022.
These data were used, along with measurements of Kr/Ar and N2/Ar ratios in stored dissolved gas samples from the Transient Tracers in the Ocean (TTO) program, to model simulations of these tracers using the Transport Matrix Method (TMM). Together these new measurements and model simulations provide insight into physical processes governing gas exchange in the high-latitude regions of North Atlantic Deep Water formation, and a comparison of physical simulations of N2/Ar ratios to observations in TTO samples reveals excess N2 that arises from benthic denitrification in the deep North Atlantic.
For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/890342NSF Division of Ocean Sciences (NSF OCE) OCE-2122427, NSF Division of Ocean Sciences (NSF OCE) OCE-192391