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A block copolymer and its preparation method and ultrafiltration membrane and its preparation method
No full text本发明提供了一种嵌段共聚物及其制备方法和超滤膜及其制备方法，属于嵌段共聚物合成领域。本发明通过异丁酰溴封端的聚砜与酰胺单体反应制备了嵌段共聚物，所述嵌段共聚物为ABA结构的三嵌段共聚物，所述ABA结构中的A段为聚酰胺，B段为异丁酰溴封端的聚砜。本发明还通过嵌段共聚物配置成铸膜液，所述铸膜液经过相转化制备成超滤膜；所述超滤膜为多孔结构，具有两亲性，在富集过程中抗污染、防堵塞；所述超滤膜为双层结构，分为截留层和支撑层。本发明所述超滤膜孔隙率高，在海水富集过程中的水通量高，富集倍数高，抗污染能力强，解决了孔径易堵塞的问题，能实现海水中胶体态物质的快速分离和富集
The present invention relates to shipborne on-site seawater filtration pretreatment equipment
No full text本实用新型涉及海水过滤装置，具体地说是一种船载现场海水过滤前处理装置，包括箱体及过滤组件，箱体上密封安装有能够推拉的过滤组件，过滤组件包括承载网及过滤棉，承载网能够相对箱体进行推拉，过滤棉附着在承载网上，通过更换承载网上过滤棉的过滤精度进而更换处理装置的过滤精度；过滤组件上方的箱体上分别设有带开关的宝塔进水口及宝塔堵头，过滤组件下方的箱体上分别设有带开关的宝塔出水口A及带开关的宝塔出水口B。本实用新型可以实现船载现场原位过滤，而且过滤精度可以根据自己的需求随意切换，过滤精度较高
The present invention relates to a method for high-sensitively detecting cyanide at normal temp. and its nano colorimetric analysis kit
No full text本发明公开了一种在常温下高灵敏检测氰化物的方法及纳米比色分析试剂盒，属于化学检测技术领域。该方法包括以下步骤：(1)制备并纯化、浓缩AuNBPs胶体溶液；(2)向Tris‑NaOH缓冲液中添加TCEP、浓缩的AuNBPs胶体溶液、CTAB和实际样品，于25℃反应40min，检测溶液的紫外吸收光谱图；(3)根据金纳米双锥体的轴向LSPR峰位移与CN‑浓度对数形式的线性方程计算实际样品中CN‑的含量。本发明的有益之处在于：(1)通过引入TCEP，大幅度的提高了CN‑检测的灵敏度(大约提高了两个数量级)；(2)使用TCEP加速反应进行，避免了加热问题，扩宽了纳米比色传感器的使用范围
The invention relates to a method for detecting tumor MSI mismatch repair protein based on multi-channel Raman probe
No full text本发明公开了一种基于多通道拉曼探针肿瘤MSI错配修复蛋白的检测方法，属于蛋白检测技术领域。该检测方法包括以下步骤：(1)将多通道拉曼分子SEE、STE、SPE和MDBM组装成O‑GERTs；(2)选取针对MLH1、MSH2、MSH6和PMS2靶向的单克隆抗体对O‑GERTs进行修饰；(3)用抗体修饰后的拉曼探针对错配修复蛋白进行正交缝隙增强拉曼检测。本发明的有益之处在于：本发明提供的检测方法通过使用一组多通道O‑GERTs，可以实现单次对MLH1、MSH2、MSH6和PMS2四种错配修复蛋白同时进行检测，极大提升了检测效率和精度
A nitrogen content monitoring device
No full text本实用新型涉及连续流动分析仪辅助设备技术领域，具体的公开了一种含氮量监测装置，包括防护外壳，所述防护外壳内安装有处理罐，所述防护外壳端部设置有连通着处理罐的酸碱中和组件，所述防护外壳上设置有用于向处理罐内进行水体交换的水箱组件，所述处理罐内侧设置有用于混合样本的混合机构；本实用新型通过导料管向处理罐内导入原料，通过水箱组件向处理罐内导入水体，此时利用驱动电机带动转动轴与过滤环网在处理罐内旋转，将土壤和植物原料混合均匀，通过PH值传感器测量混合液PH值，可通过酸碱中和组件向处理罐内导入酸碱液进行中和，使其呈中性，利用监测组件进行混合液中的含氮量监测
Design and Synthesis of Fluorine-Containing Embedded Carbon Dots Stationary Phase for Separation of Versatile Analytes
No full textThus far, numerous new stationary phases have been developed. A fluorine-containing embedded carbon dots (F3-CDs-SiO2) stationary phase was first designed and synthesized. The resulting F3-CDs-SiO2 stationary phase was characterized carefully by scanning electron microscopy, transmission electron microscopy, elemental analysis, X-ray photoelectron spectroscopy, and Brunauer-Emmett-Teller experiment. The F3-CDs-SiO2 stationary phase was slurry packed into the HPLC column (150 x 2.1 mm) for evaluation. Furthermore, the F3-CDs-SiO2 column was successfully used for separation of pesticides, nucleosides, sulfonamides, alkaloids, and alkylbenzenes. The retention mechanism (including hydrophobic interaction, F-F, hydrogen bond interaction, ion-exchange, dipole-dipole interaction, electrostatic interaction, etc.) was investigated carefully. Meanwhile, the F3-SiO2 stationary phase was synthesized and used to evaluate the role of CDs. Furthermore, various commercial stationary phases (including amino-SiO2, diol-SiO2, C18-SiO2, and PFP-SiO2) were used for comparison. Moreover, the F3-CDs-SiO2 column possessed good repeatability, reproducibility, and stability in separation of versatile analytes
Root endodermal suberization induced by nitrate stress regulate apoplastic pathway rather than nitrate uptake in tobacco (Nicotiana tabacum L.)
No full textNitrogen levels and distribution in the rhizosphere strongly regulate the root architecture. Nitrate is an essential nutrient and an important signaling molecule for plant growth and development. Hydroponic experiments were conducted to investigate the differences in endodermal suberization in tobacco (Nicotiana tabacum L.) roots at three nitrate levels. Nitrogen accumulation was detected in the roots, shoots, and xylem sap. Nitrate influx on the root surface was also measured using the non-invasive self-referencing microsensor technique (SRMT). RNA-Seq analysis was performed to identify the genes related to endodermal suberization, nitrate transport, and endogenous abscisic acid (ABA) biosynthesis. The results showed that root length, root-shoot ratio, nitrate influx on the root surface, and NiA and NRT2.4 genes were regulated to maintain the nitrogen nutrient supply in tobacco under low nitrate conditions. Low nitrate levels enhanced root endodermal suberization and hence reduced the apoplastic transport pathway, and genes from the KCS, FAR, PAS2, and CYP86 families were upregulated. The results of exogenous fluridone, an ABA biosynthesis inhibitor, indicated that suberization of the tobacco root endodermis had no relevance to radial nitrate transport and accumulation. However, ABA enhances suberization, relating to ABA biosynthesis genes in the CCD family and degradation gene ABA8ox1
Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis
No full textNonylphenol (NP), an endocrine disruptor, has been demonstrated to be a harmful environmental contaminant and toxic to organisms. In this study, to address concerns regarding the immunotoxicity of NP, we treated clam Ruditapes philippinarum hemocytes with NP in vitro and explored the underlying mechanisms of NP-induced extracellular traps (ETs). NP could induce the formation of hemocytes ETs in a dose-dependent manner. Transcriptomics analysis revealed changes of signaling pathway involved in immunity and energy metabolism in hemocytes after NP stimulation. In this process, both reactive oxygen species (ROS) and myeloperoxidase (MPO) were up-regulated. Moreover, mitogen-activated protein kinase (MAPK) signaling pathway was proved to be activated in the formation of NP-induced ETs, manifested as enhanced phosphorylation of extracellular signal-regulated kinase (ERK) but not p38 or c-Jun N-terminal kinase (JNK). In the presence of U0126, an ERK phosphorylation inhibitor, the NP-induced expression of NADPH oxidase enzyme (NOX) was significantly decreased, which further alleviated the ROS production and ultimately limited the release of ETs. NP exposure increased glucose uptake, along with enhanced activities of glycolysis-related enzymes such as hexokinase (HK) and pyruvate kinase (PK). After inhibiting glycolysis by the inhibitor 2-DG, the formation of NP-induced ETs was significantly suppressed. ERK could regulate mTOR signaling and the PI3K/AKT pathway, potentially directing ETs formation by orchestrating the glycolysis through the activation of key transcription factors c-Myc and HIF-1 alpha. Collectively, the results preliminary confirm that the ERK-NOX-ROS axis and glycolysis are involved in NP-induced ETs formation, contributing to the cellular immunotoxicity in clam
Synergistic corrosion inhibition of 4-hydroxypyridine and halogen ions: Insights from interfacial nonlinear spectroscopy
No full text4-Hydroxypiridine (4-HPy) is a green chemistry corrosion inhibitor for low-carbon steel, valued for its environmental compatibility and low toxicity. Despite lower initial effectiveness than 4-mercapto/4-aminopyridine, 4-HPy's performance is markedly enhanced by halogen ions. By employing second harmonic generation (SHG) spectroscopy combined with electrochemical methods, Raman spectroscopy, atomic force microscopy, x-ray photoelectron spectroscopy, and in situ UV spectroscopy, this study elucidates the synergistic enhancement mechanism of 4-HPy with Cl-, Br-, and I- in 0.5 mol/L HCl solution. Time-dependent SHG measurements showed a two-step process of rapid adsorption and subsequent orientation change, with a proposed mechanism to interpret the temporal changes in SHG intensity. Deducing the adsorption kinetic equations and their application to the experimental data yields the adsorption rate (k(ad)) and orientation change rate (K-re). Halogens reduce the orientation angle of 4-HPy, facilitating its adsorption on the substrate surface and effectively inhibiting corrosion via distinct mechanisms. Cl- and Br- ions primarily adsorb onto the metal surface, forming an adsorption film that not only enhances the subsequent adsorption of 4-HPy but also provides a protective effect for the metal surface. Conversely, I- forms mainly complexes with 4-HPy in solution, co-adsorbs onto the metal surface, and demonstrates a significant synergistic effect. This study revealed the synergistic efficacy hierarchy among halogen ions, with the order 4HPy + NaCl < 4HPy + NaBr < 4HPy + NaI. This study enhances our molecular-level understanding of the synergistic mechanism between halogen ions and corrosion inhibitors and provides valuable insights for designing and developing effective corrosion inhibitors
Research progress and application of chitosan dressings in hemostasis: A review
No full textHemorrhage affects human health, and severe bleeding remains a leading contributor to trauma-related mortality. The speed and effectiveness of the application of hemostatic materials are critical. Conventional hemostatic dressings such as bandages and gauze are gradually being replaced by new types of hemostatic dressings due to their poor hemostatic and antibacterial properties. Chitosan, a biopolymer, is biodegradable and nontoxic and possesses hemostatic and antibacterial properties. Chitosan induces hemostasis through direct contact with red corpuscles and platelets, independent of the coagulation pathways of the host, rendering it an optimal hemostatic dressing. It is widely used in wound care, particularly to stop bleeding, promote wound healing, and provide antimicrobial properties. This article reviews the recent research and development of chitosan-based hemostatic dressings, focusing on trauma hemostasis, burn hemostasis, diabetic skin ulcer hemostasis and other aspects. It also emphasizes the significance of chitosan dressings in wound hemostasis and healing, identifies their research opportunities in hemostasis and wound healing, and explores new research directions