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An antibacterial peptide derived from urechis unicinctus, mutant and application thereof
No full text本发明公开了一种天然抗菌肽及其在抗菌方面的应用。本发明的抗菌肽来源于单环刺螠。通过检索单环刺螠基因数据库，筛选分析获得抗菌肽成熟肽序列，其氨基酸序列如序列表中SEQ ID NO : 1所示。通过对比分析，该天然抗菌肽与目前已知所有抗菌肽的氨基酸序列存在明显差异，属于一种新型抗菌肽。抗菌实验结果表明，本发明的抗菌肽对革兰氏阳性细菌、革兰氏阴性细菌和真菌具有极强的抗菌活性，抗菌作用广谱高效，可用于制备抗菌、抑制细菌生长的药物、防腐剂、兽药、动物饲料以及化妆品
Soil sampling device for repairing wetland suaeda salsa planting substrate
No full text本发明专利涉及土壤修复检测采样的技术领域，具体为湿地碱蓬种植底质修复用土壤采样装置，包括行走机构、取样机构和探测机构；行走机构包括基座和安装在基座左右两侧的多个支腿结构，每个支腿结构外侧面均设有一取样机构；取样机构包括动力单元、取样筒和收纳筒，取样筒为轴向贯通的套筒结构，动力单元一端安装于取样筒内，动力单元包括伸出和回缩两个状态，动力单元在伸出状态向回缩状态切换这个过程中，动力单元的底部将其正下方的土壤吸入取样筒内；本发明使用时只需要每次画一个点位，就能对其附近的多个固定距离延伸点位进行同时采样，提升了采样效率，并且由于施工人员在湿地上面行走较为困难，本采样装置能够自行在湿地上表面行走
A kind of nucleic acid aptamer that recognizes protopolyalginic acid-1 and its application
No full text本发明属于分子生物学技术领域，特别涉及一种识别原多甲藻酸‑1的核酸适配体及其应用。核酸适配体包括下列选择。(1)：AZA‑Apt‑02所示的核苷酸序列；或(2)：AZA‑Apt‑09所示的核苷酸序列；或(3)：AZA‑Apt‑23所示的核苷酸序列；或(4)：AZA‑Apt‑23b48所示的核苷酸序列；或(5)：具有与(1)或(2)或(3)或(4)的序列至少90%同源性的核苷酸序列；或(6)：(1)、(2)、(3)、(4)的序列或具有与(1)或(2)或(3)或(4)的序列至少90%同源性的核苷酸序列被化学修饰的核酸序列。本发明的核酸适配体可作为一种有效的分子识别元件
Effects of different soil water matric potentials on growth traits and yield characteristics of sunflower (<i>Helianthus annuus</i> Linn.) under drip irrigation in a salinized farmland in northern China
No full textCrop irrigation based on controlling the matric potential of soil water at the depth of soil fixation in the root zone is more suitable than deficit irrigation for promoting cultivation in arid agricultural areas. However, it remains challenging to determine how controlled irrigation (CI) quantifies crop the response to water limitation and to establish the appropriate CI thresholds based on soil water matric potential. This study was conducted on sunflower fields in Hetao Irrigation District, a typical arid agricultural area in China where this crop is widely grown. Five CI treatments were applied for different soil water matric potentials (-8, -16, -24, -32, and - 40 kPa) to study the effects of CI on the soil environment and the relationship with crop yield. The following conclusions were drawn: (1) after 2 years of CI treatments, secondary salinization was significantly reduced below the defined criteria for saline soils (4 dS/m), achieving desalination of the entire soil profile; (2) the application of CI as an irrigation strategy in the management of sunflower fields significantly improved physiological traits during the crucial period of sunflower yield formation by increasing plant height while reducing stem thickness; (3) two treatments with the greatest difference in the lower limit of control of soil water matric potential under this irrigation strategy exhibited a yield variance ratio that increased from 0.14% in the first year to 15.37% in the second year; and (4) the treatment that controlled soil water matric potential at -24 kPa obtained the highest yield of all treatments in the test cycle, leading to an increase in seed yield of up to 1266.66 kg/ha in 2022 compared to the previous year
Effects of seawater acidification and warming on morphometrics and biomineralization-related gene expression during embryo-larval development of a lightly-calcified echinoderm
No full textCO2-induced ocean acidification and warming pose ecological threats to marine life, especially calcifying species such as echinoderms, who rely on biomineralization for skeleton formation. However, previous studies on echinoderm calcification amid climate change had a strong bias towards heavily calcified echinoderms, with little research on lightly calcified ones, such as sea cucumbers. Here, we analyzed the embryo-larval development and their biomineralization-related gene expression of a lightly calcified echinoderm, the sea cucumber (Apostichopus japonicus), under experimental seawater acidification (OA) and/or warming (OW). Results showed that OA (- 0.37 units) delayed development and decreased body size (8.58-56.25 % and 0.36-19.66 % decreases in stage duration and body length, respectively), whereas OW (+3.1 degrees C) accelerated development and increased body size (33.99-55.28 % increase in stage duration and 2.44-14.41 % enlargement in body length). OW buffered the negative effects of OA on the development timing and body size of A. japonicus. Additionally, no target genes were expressed in the blastula stage, and only two biomineralization genes (colp3 alpha, cyp2) and five TFs (erg, tgif, foxN2/3, gata1/2/3, and tbr) were expressed throughout the embryo-larval development. Our findings suggest that the low calcification in A. japonicus larvae may be caused by biomineralization genes contraction, and low expression of those genes. Furthermore, this study indicated that seawater acidification and warming affect expression of biomineralization-related genes, and had an effect on body size and development rate during the embryo-larval stage in sea cucumbers. Our study is a first step toward a better understanding of the complexity of high pCO(2) on calcification and helpful for revealing the adaptive strategy of less-calcified echinoderms amid climate change
Nonfluorescent Near-Infrared Surface-Enhanced Resonance Raman Nanoprobes with Ultrahigh Brightness and Synergistic Photothermal Effect
No full textNear-infrared (NIR) surface-enhanced resonance Raman (SERRS) nanoprobes have found wide applications in biomedicine; however, almost all of these nanoprobes are fluorescent because the resonant Raman dyes used cannot be fully quenched onto the underlying plasmonic nanoparticles. Therefore, suppressing the fluorescence backgrounds in resonant Raman spectroscopy imaging is extremely important. In this work, we use a black hole quencher, IQ1, as a Raman dye to develop absolutely nonfluorescent NIR resonant SERRS NPs. Ultrafast spectroscopy clarifies that the nonfluorescent mechanism of the dyes is attributed to the ultrafast internal conversion at the subpicosecond scale, which quenches the fluorescence of excited states. The resultant nanoprobes exhibit zero fluorescent background, femtomolar-level sensitivity (100 fM) as well as superb photostability (tau = 10006 s) without fluorescence photobleaching, outperforming that of fluorescent counterparts. More importantly, the SERRS NPs show a synergistic photothermal effect originating from the dye molecule-plasmon interactions, achieving a high photothermal conversion efficiency of 64.94%. Featuring these excellent properties, these SERRS NPs allow for longitudinally photostable cellular imaging and enhanced photothermal elimination of cancer cells. To the best of our knowledge, this is the first example of absolutely nonfluorescent NIR SERRS NPs, opening up promising applications for improved phototheranostics
Nonfluorescent Near-Infrared Surface-Enhanced Resonance Raman Nanoprobes with Ultrahigh Brightness and Synergistic Photothermal Effect
No full textNear-infrared (NIR) surface-enhanced resonance Raman (SERRS) nanoprobes have found wide applications in biomedicine; however, almost all of these nanoprobes are fluorescent because the resonant Raman dyes used cannot be fully quenched onto the underlying plasmonic nanoparticles. Therefore, suppressing the fluorescence backgrounds in resonant Raman spectroscopy imaging is extremely important. In this work, we use a black hole quencher, IQ1, as a Raman dye to develop absolutely nonfluorescent NIR resonant SERRS NPs. Ultrafast spectroscopy clarifies that the nonfluorescent mechanism of the dyes is attributed to the ultrafast internal conversion at the subpicosecond scale, which quenches the fluorescence of excited states. The resultant nanoprobes exhibit zero fluorescent background, femtomolar-level sensitivity (100 fM) as well as superb photostability (tau = 10006 s) without fluorescence photobleaching, outperforming that of fluorescent counterparts. More importantly, the SERRS NPs show a synergistic photothermal effect originating from the dye molecule-plasmon interactions, achieving a high photothermal conversion efficiency of 64.94%. Featuring these excellent properties, these SERRS NPs allow for longitudinally photostable cellular imaging and enhanced photothermal elimination of cancer cells. To the best of our knowledge, this is the first example of absolutely nonfluorescent NIR SERRS NPs, opening up promising applications for improved phototheranostics
Crude Oil Biodegradation by a Biosurfactant-Producing Bacterial Consortium in High-Salinity Soil
No full textBioremediation is a promising strategy to remove crude oil contaminants. However, limited studies explored the potential of bacterial consortia on crude oil biodegradation in high salinity soil. In this study, four halotolerant strains (Pseudoxanthomonas sp. S1-2, Bacillus sp. S2-A, Dietzia sp. CN-3, and Acinetobacter sp. HC8-3S), with strong environmental tolerance (temperature, pH, and salinity), distinctive crude oil degradation, and beneficial biosurfactant production, were combined to construct a bacterial consortium. The inoculation of the consortium successfully degraded 97.1% of total petroleum hydrocarbons in 10 days, with notable removal of alkanes, cycloalkanes, branched alkanes, and aromatic hydrocarbons. Functional optimization showed that this consortium degraded crude oil effectively in a broad range of temperature (20-37 degrees C), pH (6-9), and salinity (0-100 g/L). In salt-enriched crude-oil-contaminated soil microcosms, the simultaneous treatment of bioaugmentation and biostimulation achieved the highest crude oil degradation rate of 568.6 mg/kg/d, compared to treatments involving abiotic factors, natural attenuation, biostimulation, and bioaugmentation after 60 days. Real-time PCR targeting the 16S rRNA and alkB genes showed the good adaptability and stability of this consortium. The degradation property of the constructed bacterial consortium and the engineered consortium strategy may have potential use in the bioremediation of crude oil pollution in high-salinity soil
Crude Oil Biodegradation by a Biosurfactant-Producing Bacterial Consortium in High-Salinity Soil
No full textBioremediation is a promising strategy to remove crude oil contaminants. However, limited studies explored the potential of bacterial consortia on crude oil biodegradation in high salinity soil. In this study, four halotolerant strains (Pseudoxanthomonas sp. S1-2, Bacillus sp. S2-A, Dietzia sp. CN-3, and Acinetobacter sp. HC8-3S), with strong environmental tolerance (temperature, pH, and salinity), distinctive crude oil degradation, and beneficial biosurfactant production, were combined to construct a bacterial consortium. The inoculation of the consortium successfully degraded 97.1% of total petroleum hydrocarbons in 10 days, with notable removal of alkanes, cycloalkanes, branched alkanes, and aromatic hydrocarbons. Functional optimization showed that this consortium degraded crude oil effectively in a broad range of temperature (20-37 degrees C), pH (6-9), and salinity (0-100 g/L). In salt-enriched crude-oil-contaminated soil microcosms, the simultaneous treatment of bioaugmentation and biostimulation achieved the highest crude oil degradation rate of 568.6 mg/kg/d, compared to treatments involving abiotic factors, natural attenuation, biostimulation, and bioaugmentation after 60 days. Real-time PCR targeting the 16S rRNA and alkB genes showed the good adaptability and stability of this consortium. The degradation property of the constructed bacterial consortium and the engineered consortium strategy may have potential use in the bioremediation of crude oil pollution in high-salinity soil
Highly Monodisperse Stable Gold Nanorod Powder for Optical Sensor
No full textGold nanorods (GNRs) as plasmonic metal nanoparticles are valuable for optical applications due to their tunable plasmonic properties. However, conventional colloidal GNRs face significant optical instability during storage, which limits their practical use. In this work, we developed a highly dispersible GNR powder using an octadecyl trimethylammonium bromide (C18TAB)-assisted freeze-drying method, preserving the optical and chemical sensing properties of GNRs for over 4 months. Compared with C16TAB, C18TAB significantly enhances the GNRs dispersibility even at lower concentrations. Our study demonstrates that C18TAB forms a sponge-like crystal structure that prevents aggregation during the freeze-drying process. The resulting GNR powder retains its plasmonic features and water dispersibility, achieving near-identical optical properties to those of fresh GNR solutions. This stability enabled creation of a liquid-free colorimetric test kit with a long shelf life. This work marks a significant step forward in the use of GNRs as standard analytical reagents, opening new avenues for real-world applications