Institutional Repository of Yantai Institute of Coastal Zone Research, CAS
Not a member yet
13079 research outputs found
Sort by
Magnetic multi-template molecularly imprinted polymers for selective simultaneous extraction of chlorophenols followed by determination using HPLC
No full textMagnetic multi-template molecularly imprinted polymers (M-mt-MIPs) were successfully synthesized by surface imprinting and multi-template imprinting strategy, using polydopamine coated magnetic multi-walled carbon nanotubes as supporting materials, five typical chlorophenols (CPs) as templates, methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker. Compared to non-imprinted polymers (NIPs), the as-prepared M-mt-MIPs showed high adsorption capacity (32.58-80.63 mg g- 1), rapid mass transfer and specific selectivity for the five targeted CPs, which were applied as magnetic solid-phase extraction (MSPE) adsorbents. Parameters affecting MSPE efficiency were detailed investigated, such as adsorbents dosage, sample pH, extraction time, type and volume of desorption solvent and salt effect. Combined with HPLC-DAD, a simple, rapid and sensitive method was established, showing good linearity (2-200 mu g L- 1), low limits of detection (0.32-0.49 mu g L- 1), and high enrichment factors (35.2-108). The developed M-mt-MIPs-MSPE-HPLC method was applied to enrich and determine CPs in tannery wastewater, wet-blue and crust leather, and satisfactory spiking recoveries were attained in the range of 73.95-109.7% with relative standard deviations (RSDs) of 2.13-8.48%. This study provided a new alternative material and method to rapid simultaneously extract and analyze low concentration of typical CPs in complicated matrices
Magnetic multi-template molecularly imprinted polymers for selective simultaneous extraction of chlorophenols followed by determination using HPLC
No full textMagnetic multi-template molecularly imprinted polymers (M-mt-MIPs) were successfully synthesized by surface imprinting and multi-template imprinting strategy, using polydopamine coated magnetic multi-walled carbon nanotubes as supporting materials, five typical chlorophenols (CPs) as templates, methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linker. Compared to non-imprinted polymers (NIPs), the as-prepared M-mt-MIPs showed high adsorption capacity (32.58-80.63 mg g- 1), rapid mass transfer and specific selectivity for the five targeted CPs, which were applied as magnetic solid-phase extraction (MSPE) adsorbents. Parameters affecting MSPE efficiency were detailed investigated, such as adsorbents dosage, sample pH, extraction time, type and volume of desorption solvent and salt effect. Combined with HPLC-DAD, a simple, rapid and sensitive method was established, showing good linearity (2-200 mu g L- 1), low limits of detection (0.32-0.49 mu g L- 1), and high enrichment factors (35.2-108). The developed M-mt-MIPs-MSPE-HPLC method was applied to enrich and determine CPs in tannery wastewater, wet-blue and crust leather, and satisfactory spiking recoveries were attained in the range of 73.95-109.7% with relative standard deviations (RSDs) of 2.13-8.48%. This study provided a new alternative material and method to rapid simultaneously extract and analyze low concentration of typical CPs in complicated matrices
High-yield magnetosome production of <i>Magnetospirillum magneticum</i> strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation
No full textObjectives Developing a simplified flask fermentation strategy utilizing magnetotactic bacterium AMB-1 and optimized iron supplementation for high-yield magnetosome production to address the challenges associated with magnetosome acquisition.
Results A reliable processing for the pure culture of AMB-1 was established using standard laboratory consumables and equipment. Subsequently, the medium and iron supplementation were optimized to enhance the yield of AMB-1 magnetosomes. The mSLM supported higher biomass accumulation in flask fermentation, reaching an OD565 of similar to 0.7. The premixed solution of ferric quinate and EDTA-Fe (at a ratio of 0.5:0.5 and a concentration of 0.4 mmol/L) stabilized Fe3+ and significantly increased the reductase activity of AMB-1. Flask fermentations with an initial volume of 15 L were then conducted employing the optimized fermentation strategy. After two rounds of iron and nutrient supplementation, the magnetosome yield reached 185.7 +/- 9.5 mg/batch (approximately 12 mg/L), representing the highest AMB-1 flask fermentation yield to our knowledge.
Conclusion A flask fermentation strategy for high-yield magnetsome production was developed, eliminating the need for bioreactors and greatly simplifying the process of magnetosome acquisition
Food shortage driven crop residue burning and health risk
No full textEnding global hunger is one of the greatest challenges humanity faces. The second Sustainable Development Goal (SDG 2), Zero Hunger, seeks to simultaneously address global environmental sustainability and food security challenges. However, ending hunger and food shortage would lead to the increase of agricultural activities and crop yields, which in turn might rise crop residue biomass burning, leading to adverse environmental and health consequences. This study estimated the health risk using modeled airborne benzo[a]pyrene (BaP), a common indicator of carcinogenic polycyclic aromatic hydrocarbons (PAH) released from crop residue biomass burning, and demonstrated the dependence of incremental lifetime lung cancer risk (ILCR) on the prevalence of undernourishment (PoU). Results showed that 10.4% of the global population are exposed to BaP concentration levels exceeding the EU target value (1 ng/m(3)) due to crop residue burning. The dependence of ILCR on PoU was attributed to increasing post-harvest crop residue biomass burning consequent to growing crop yield motivated by food shortage. Although global mean ILCR from crop residue burning BaP emission was positively correlated with the mean PoU at a correlation coefficient of r = 0.87 (p < 0.0001), positive and negative associations between PoU and ILCR were found among different less-developed countries. Eighty-nine less-developed countries were clustered into five groups, and the response of each group's ILCR to their respective PoU was assessed, which was interpreted by a human development index (HDI) that measured key dimensions of human development. Results also revealed that sustainable agriculture and implementation of air emission control measures play key roles in the association between hunger and ILCR
Food shortage driven crop residue burning and health risk
No full textEnding global hunger is one of the greatest challenges humanity faces. The second Sustainable Development Goal (SDG 2), Zero Hunger, seeks to simultaneously address global environmental sustainability and food security challenges. However, ending hunger and food shortage would lead to the increase of agricultural activities and crop yields, which in turn might rise crop residue biomass burning, leading to adverse environmental and health consequences. This study estimated the health risk using modeled airborne benzo[a]pyrene (BaP), a common indicator of carcinogenic polycyclic aromatic hydrocarbons (PAH) released from crop residue biomass burning, and demonstrated the dependence of incremental lifetime lung cancer risk (ILCR) on the prevalence of undernourishment (PoU). Results showed that 10.4% of the global population are exposed to BaP concentration levels exceeding the EU target value (1 ng/m(3)) due to crop residue burning. The dependence of ILCR on PoU was attributed to increasing post-harvest crop residue biomass burning consequent to growing crop yield motivated by food shortage. Although global mean ILCR from crop residue burning BaP emission was positively correlated with the mean PoU at a correlation coefficient of r = 0.87 (p < 0.0001), positive and negative associations between PoU and ILCR were found among different less-developed countries. Eighty-nine less-developed countries were clustered into five groups, and the response of each group's ILCR to their respective PoU was assessed, which was interpreted by a human development index (HDI) that measured key dimensions of human development. Results also revealed that sustainable agriculture and implementation of air emission control measures play key roles in the association between hunger and ILCR
A Nanofluorescent Probe for Evaluating the Fluctuation of Aminopeptidase N in Nonalcoholic Fatty Liver Disease and Hepatic Fibrosis
No full textAminopeptidase N (APN/CD13) is a widely expressed transmembrane ectoenzyme that is crucial for maintaining normal physiological activities. It exhibits abnormal activity closely associated with hepatic fibrosis and nonalcoholic fatty liver disease (NAFLD). Therefore, there is a high demand for noninvasive detection of aminopeptidase N (APN) in the diagnosis and research of related diseases. Here, we developed a small molecule fluorescent probe, Hcy-APN, which is a fluorescent probe with high sensitivity and selectivity for the detection of APN. Furthermore, we synthesized the fluorescent nanoprobe Hcy-APN@MSN by self-assembling Hcy-APN and mesoporous silica nanoparticles in solution using a combination of molecular probe design and nanofunctionalization strategies. The detection limit of this probe was 1.5 ng/mL. Hcy-APN@MSN exhibits more stable spectral characteristics compared to Hcy-APN and is suitable for detecting APN activity in live cells and mice. Hcy-APN@MSN was utilized for in vivo and intracellular imaging of NAFLD and hepatic fibrosis at different stages, as well as for a systematic assessment of APN levels in the liver. The results confirm an elevation in the expression levels of APN in NAFLD and hepatic fibrosis models. Furthermore, we investigated the inhibitory effect of the APN inhibitor bestatin in nonalcoholic fatty liver and hepatic fibrosis disease models, confirming its regulatory effect on APN levels in cells and in vivo in both disease models. Therefore, this study may offer diagnostic possibilities for detecting NAFLD and hepatic fibrosis
Turnover structures in macrobenthic communities rather than nestedness in the Yellow River Delta wetland, China
No full textComprehending the triggers, such as turnover and nestedness, in structuring of macrobenthic communities is crucial for informing wetland biodiversity conservation strategies. Previous studies on macrobenthic communities in the Yellow River Delta wetland lacked beta-diversity analysis and did not consider the role of turnover and nestedness in different ecological zones. Therefore, we analyzed taxonomic, phylogenetic and functional alpha- and beta-diversities in the Yellow River Delta wetland, intertidal and offshore zones. The study revealed that the intertidal zone had higher polychaete abundance of species, while the offshore zone was dominated by a more mobile crustaceans and fish species. The findings of this study showed that alpha-diversity metrics for taxonomic, phylogenetic and functional dimensions were higher in offshore stations, whereas beta-diversity was higher in intertidal communities. Turnover contributed more significantly to taxonomic, phylogenetic and functional beta-diversities, highlighting changes not only happened in species composition but also in phylogenetic relationships and functional traits between the two zones. Additionally, the intertidal zone exhibited higher turnover, while the offshore zone had an increased proportion of nestedness component contribution, especially for functional beta-diversity. Correlation analysis indicated significant associations among three beta-diversities and their respective turnover and nestedness components. The correlation between taxonomic and phylogenetic aspects was stronger compared to the correlations between taxonomic and functional aspects as well as between functional and phylogenetic aspects. These findings enhance our understanding of the drivers of biodiversity in macrobenthic communities and underscore the importance of considering phylogenetic and functional dimensions in beta-diversity studies
Real-world emission characteristics of VOCs from typical cargo ships and their potential contributions to secondary organic aerosol and O<sub>3</sub> under low-sulfur fuel policies
No full textMandatory use of low-sulfur fuel according to the global sulfur limit regulation has reduced the emissions of SO2 and particulate matter (PM) significantly on ships, and it also leads to a very large uncertainty in volatile organic compound (VOC) emissions. Therefore, an on-board test of VOCs from nine typical cargo ships with low-sulfur fuels in China was carried out in this study. Results showed that the emission factor of VOCs (EFVOCs) varied largely from 0.09 to 3.01 g kg(-1) of fuel, with domestic coastal cargo ships (CCSs) having the highest level, followed by inland cargo ships (ICSs) and ocean-going vessels (OGVs). The switch of fuels from heavy fuel oil (HFO) to diesel increased EFVOCs by 48 % on average, which enhanced both O-3 and secondary organic aerosol (SOA) formation potential, especially for OGVs. Besides, the use of low-sulfur fuels for OGVs also led to a significant increase in naphthalene emission. These indicated that the implementation of a global ultra-low-sulfur oil policy in the near future needs to be optimized. Moreover, aromatics were the most important common contributors to O-3 and SOA in ship exhausts, which need to be controlled as a matter of priority. It was also found that the benzene, toluene, and ethylbenzene ratio of 0.5: 0.3: 0.2 on average could be considered a diagnostic characteristic to distinguish ship emissions from other emission sources
Nitrogen fixation and calcium activation of oyster shell during composting and its performance on acidic soil amendment
No full textChicken manure, corn straw and oyster shell powder (OSP) were used as raw materials for organic-inorganic combined composting. Experiments were designed by adding different amounts of OSP (0 %, 20 %, and 40 %), designed as CK, T1, and T2, respectively. In final composts, the pH value increased to 8.72-9.08, and the electrical conductivity ranged from 2.08-3.82 mS center dot cm(-1). The co-composting could effectively reduce ammonia emissions by 54.65 % and could activate the calcium component by 37.5 %. Addition of OSP promoted the humification and the humic acid could increased 86.1 %. This calcium-based compost of oyster shells (OSs) activated by composting was used to plant Brassica napus by pot experiment. Compared with the addition of OSP after composting, the calcium-based compost activated by composting significantly promoted the growth of Brassica napus because of more nitrogen, humus and available calcium in the calcium-based compost. In addition, this calcium-based compost significantly increased pH, organic matter (OM) and exchangeable calcium content in the acidic soil. In general, composting is an effective method to realize the fertilizer utilization of OSs. This composting process could make full use of calcium in oyster shells to achieve nitrogen retention and humification enhancement during composting, meanwhile calcium morphology in oyster shells can be activated and high-quality acidic soil conditioner can be obtained
Preparation and properties of Eu3+doped tungstate red phosphor powders
No full textNaY(WO4)2:Eu3+ phosphors were synthesized using high-temperature solid state method. The effects of Eu3+ doping concentrations on structure and photoluminescence properties of the powders were investigated. The results indicate that the pure NaY(WO4)2:Eu3+ phase can be produced by calcining at 800 degrees C where Eu3+ doping does not alter the main phase structure. Eu3+ ions were successfully incorporated into the crystal lattice, replacing Y3+ at the B-site. This caused gradual shifts of the characteristic X-ray diffraction peaks to lower 2 theta angles. Morphological characterization revealed that the sample particles had irregular polygonal shapes with sizes ranging from 100 to 300 nm. There was minimal variation in morphology among samples with different doping concentrations. The samples exhibited the characteristic spectral emission of Eu3+ and emitted red light at a wavelength of 616 nm when excited by 394 nm near-ultraviolet light. The luminescence intensity of the nanophosphor gradually increased with increasing Eu3+ doping concentration, reaching its maximum at a doping ratio of 0.6, followed by a decrease due to the concentration quenching. The quenching mechanism was analysed to be primarily dipole-dipole interaction, in accordance with the Dexter's theory of doping concentration. The luminescence positions of the phosphors slightly changed with varying Eu3+ doping concentrations, and the colour coordinates closely matched the standard value of the red colour in the National Television Standards Committee, confirming the stability of the obtained powders as red phosphors