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Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira
No full textCyclic diguanosine monophosphate (c-di-GMP) is a second messenger in bacteria that regulates multiple biological functions, including biofilm formation, virulence, and intercellular communication. However, c-di-GMP signaling is virtually unknown in economically important filamentous cyanobacteria, Arthrospira. In this study, we predicted 31 genes encoding GGDEF-domain proteins from A. platensis NIES39 as potential diguanylate cyclases (DGCs). Phylogenetic distribution analysis showed five genes (RS09460, RS04865, RS26155, M01840, and E02220) with highly conserved distribution across 25 Arthrospira strains. Adc1 encoded by RS09460 was further characterized as a typical DGC. By establishing the genetic transformation system of Arthrospira, we demonstrated that the overexpression of Adc1 promoted the production of extracellular polymeric substances (EPS), which in turn caused the aggregation of filaments. We also confirmed that RS04865 and RS26155 may encode active DGCs, while enzymatic activity assays showed that proteins encoded by M01840 and E02220 have phosphodiesterase (PDE) activity. Meta-analysis revealed that the expression profiles of RS09460 and RS04865 were unaffected under 31 conditions, suggesting that they may function as conserved genes in maintaining the basal level of c-di-GMP in Arthrospira. In summary, this report will provide the basis for further studies of c-diGMP signal in Arthrospira
Spatial distribution of soil organic carbon across diverse vegetation types in a tidal wetland
No full textTidal wetlands are significant contributors to global "blue carbon" resources. The water and salt gradients in tidal wetlands shape vegetation distribution and store significant amounts of soil organic carbon (SOC). We selected four distinct regions within the intertidal zone comprising three distinct vegetation types: low-tide saltmarsh Suaeda salsa (LS), high-tide saltmarsh Suaeda salsa (HS), mid-high-tide Phragmites australis (P), and high-tide Tamarix chinensis (T). Through field sampling and indoor analysis, we found significant differences in SOC levels across various vegetation types along the land-sea gradient. Among these, LS exhibited the highest SOC levels, while P had the lowest. Additionally, there were vertical variations of SOC within a 1-m range among different vegetation types. Mantel analysis and SEM demonstrated that SWC influences SOC content by manipulating vegetation types, thereby regulating total soil carbon. Overall, our findings provide valuable insights for further investigating the effects of vegetation succession on soil carbon pool evolution
A portable measuring device for quickly detecting sulfide is disclosed
No full text本实用新型涉及海水检测技术领域，具体公开了一种快速检测硫化物的便携式测定设备，用以解决了现有技术中携带不方便的问题；该设备包括一个测定外壳，所述测定外壳上端一侧设有一个弹性卡板，所述测定外壳内部设有一个检测腔，检测腔上端口位置滑动设有一个滑动套，所述滑动套内部滑动设有一个检测柱，所述检测柱和滑动套之间通过复位件连接，所述检测柱下端设有检测缺口。本实用新型针对现有需要进行设计，方便检测人员外出携带，提高了检测的便携性，同时还方便将样品送入和取走，在转移的过程中还设置透明防护套，避免样品四溅影响设备后续检测精度，另外通过设置内置的滴加结构，简化了检测操作，有助于提高检测效率
The present invention relates to a method for high-sensitively detecting cyanide at normal temp. and its nano colorimetric analysis kit
No full text本发明公开了一种在常温下高灵敏检测氰化物的方法及纳米比色分析试剂盒，属于化学检测技术领域。该方法包括以下步骤：(1)制备并纯化、浓缩AuNBPs胶体溶液；(2)向Tris‑NaOH缓冲液中添加TCEP、浓缩的AuNBPs胶体溶液、CTAB和实际样品，于25℃反应40min，检测溶液的紫外吸收光谱图；(3)根据金纳米双锥体的轴向LSPR峰位移与CN‑浓度对数形式的线性方程计算实际样品中CN‑的含量。本发明的有益之处在于：(1)通过引入TCEP，大幅度的提高了CN‑检测的灵敏度(大约提高了两个数量级)；(2)使用TCEP加速反应进行，避免了加热问题，扩宽了纳米比色传感器的使用范围
An ester-terminated polyethersulfone block copolymer and its preparation method and use
No full text本发明提供了一种酯基封端的聚醚砜嵌段共聚物及其制备方法和用途，属于聚醚砜嵌段共聚物技术领域。本发明通过双酚S和双(4‑氟苯基)砜聚合制备得到聚醚砜，并通过引入2‑溴异丁酰溴对所述聚醚砜进行封端，得到了改性聚醚砜，再通过丙烯酸酯单体与所述改性聚醚砜的聚合制备得到酯基封端的聚醚砜嵌段共聚物，所述酯基封端的聚醚砜嵌段共聚物的分子量高，分子量分布均匀，因此制备出的超滤膜强度高，且超滤膜的选择透过性好、水通量高，在过滤海水后水中的生化需氧量、总悬浮物、溶解氧及大肠菌群数低，说明超滤膜的分离效果好
The present invention relates to an ACE inhibiting peptide and its preparation method and application
No full text本发明涉及生物活性肽技术领域。本发明提供了一种ACE抑制肽及其制备方法和应用。本发明通过葡聚糖Sephadex G‑25凝胶层析方法，对金枪鱼肽粉进行分离纯化，选取活性最佳组分进行氨基酸序列鉴定，筛选出具有ACE抑制活性的新氨基酸序列；本发明制备的ACE抑制肽LTFSY、STHPHF降压活性突出、生物安全性好，其IC50值为20.72μg/mL、30.71μg/mL，比现有大部分ACE抑制肽活性更高；通过分子对接等现代手段初步评价其构效关系，并通过细胞实验验证其跨膜转运机制，提高了该ACE抑制肽在慢性病治疗领域的应用价值
A soil and organic material mixing device
No full text本实用新型公开了一种土壤与有机物料混匀装置，涉及农业施肥领域，包括主体，所述主体的内部位置设置有搅拌机构，所述搅拌机构包括支撑柱，所述支撑柱的顶端位置设置有横板，所述横板顶端的右侧位置设置有第一电机，所述横板底端的右侧位置设置有第一转轴，所述第一转轴侧壁的顶部与底部位置均设置有支撑圈板；本实用新型的优点在于：该装置可以对土壤与有机物料进行充分的搅拌，且便于将含有的较大颗粒绞碎，从而提高了土壤与有机物料搅拌的效果，使植物可以均匀的吸收营养，且在施肥的过程中防止了物料随风飘走，使该装置便于进行灵活的移动，且放置较稳定，使用较便捷，从而提高了实用性
Spatial effects of shrub encroachment on wetland soil pH and salinity in the Yellow River Delta, China
No full textThe encroachment of woody shrubs in grass-dominated coastal salt marsh ecosystems has potentially influenced soil physical and chemical processes at various scales. Understanding the effects of shrub encroachment on those ecosystems requires assessments of the spatial heterogeneity in soil variables across multiple scales. Combined with GIS spatial tools and sampling data, we evaluated the vertical (0-80 cm) and spatial characteristics of soil pH and soil salinity (or electrical conductivity, EC) in a coastal wetland that experienced 40 years of shrub (specifically, the Tamarix chinensis species) encroachment. In order to obtain the information of the spatial patterns and scale domains of soil pH and EC, we employed the lacunarity analysis method. Furthermore, we utilized both Mantel test and Pearson correlation to identify their relationship. The results revealed that shrub encroachment had notable impacts on the magnitude and spatial patterns of soil pH and EC. These effects were driven by specific encroaching processes. Specifically, large shrub patches (SP) resulted in a significant rise in soil pH and a noteworthy reduction in soil EC. In addition, small shrub clusters (SC) caused a notable reduction in soil EC, but did not have significant impacts on soil pH. The impacts of shrubs on soil pH were primarily observed in 0-20 cm, whereas their effects on soil EC extended to deeper soil layers (e.g., up to 60 cm). Shrub encroachment also increased the landscape heterogeneity of both soil pH and EC, with the effects being more pronounced on soil EC. The lacunarity analysis revealed that the spatial heterogeneity of soil EC differed significantly among four depths, 0-20, 20-30, 30-60, and 60-80 cm, while the heterogeneity of soil pH varied significantly between two depths of 0-60 and 60-80 cm. Furthermore, soil pH and soil EC showed a strong spatial association, with a negative relationship observed between the two variables within each soil layer. Our study at the landscape scale highlights the opposite responses of soil pH and EC in saline-alkali wetlands to shrub encroachment. By understanding the impacts of shrubs on soil properties, we can enhance our ability to effectively restore and manage these degraded ecosystems
Shining light on carbon dots: Toward enhanced antibacterial activity for biofilm disruption
No full textIn spite of tremendous efforts dedicated to addressing bacterial infections and biofilm formation, the post-antibiotic ear continues to witness a gap between the established materials and an easily accessible yet biocompatible antibacterial reagent. Here we show carbon dots (CDs) synthesized via a single hydrothermal process can afford promising antibacterial activity that can be further enhanced by exposure to light. By using citric acid and polyethyleneimine as the precursors, the photoluminescence CDs can be produced within a one-pot, one-step hydrothermal reaction in only 2 h. The CDs demonstrate robust antibacterial properties against both Gram-positive and Gram-negative bacteria and, notably, a considerable enhancement of antibacterial effect can be observed upon photo-irradiation. Mechanistic insights reveal that the CDs generate singlet oxygen (1O2) when exposed to light, leading to an augmented reactive oxygen species level. The approach for disruption of biofilms and inhibition of biofilm formation by using the CDs has also been established. Our findings present a potential solution to combat antibacterial resistance and offer a path to reduce dependence on traditional antibiotics.
Shining carbon dots (CDs) produced by a single-step hydrothermal method can afford enhanced antibacterial activity against both Gram-positive and Gram-negative bacteria. Mechanistic insights reveal that the CDs generate singlet oxygen (1O2) under light irradiation, contributing to an augmented ROS level, facilitating disruption of biofilms and inhibition of biofilm formation. imag
Immobilization of Au nanoparticles on black phosphorus modified spherical covalent organic frameworks for the flow-through reduction of 4-nitrophenol
No full textCovalent organic frameworks (COFs), due to their unique structure and morphology, manifest great potential as active substrates to support noble metals for sustainable catalytic applications. However, it is still a challenge for efficient and effective catalysis using these kinds of COFs-based catalysts. Here, black phosphorus (BP) nanosheets modified spherical COFs (SCOFs) were synthesized, followed by the immobilization of Au nanoparticles (Au NPs) to fabricate Au NPs/BP/SCOFs microspheres. The as-prepared Au NPs/BP/SCOFs microspheres were then filled in a column for the continuous catalytic reduction of 4-nitrophenol. A fast and highly efficient flowthrough catalysis of 4-nitrophenol with a reduction efficiency of 99 % under the flux of 2500 L m- 2 h-1 was obtained. Furthermore, a stable catalytic performance under flow-through process was exhibited with only 0.13 wt% of total Au NPs releasing after testing for 24 h. The strategy for designing COFs-based substrates with enhanced electron transfer and controllable morphology would develop a new route to promote the application of these kinds of COFs-based catalysts