Institutional Repository of Institute of Process Engineering, CAS (IPE-IR）
Not a member yet
40778 research outputs found
Sort by
Open Research Fund Program of Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry[CP-2020-YB4]
No full text
The fabrication of Ti4O7 particle composite modified PbO2 coating electrode and its application in the electrochemical oxidation degradation of organic wastewater
No full textA novel Ti/PbO2 electrode modified with Ti4O7 was synthesized through electrochemical deposition method. The surface morphology and electrochemical performance of the as-prepared electrodes were characterized by Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), Transmission Electron Microscope (TEM), Cyclic Voltammetry (CV), Linear Sweep Voltammetry (LSV), Electrochemical Impedance Spectroscopy (EIS), etc. The characterization results indicated that Ti4O7 composite modification improved the surface morphology and crystal plane orientation of the PbO2 coating electrodes and reduced the interplanar spacing of PbO2. Phenol was chosen as the model pollutant to evaluate electrochemical activity of the electrode. The Ti4O7 with 1.0 g L-1 below 500 mesh modification electrodes presented the highest removal efficiency (97.8%) on degradation of phenol, which could be attributed to its high oxygen evolution potential (OEP) and more electrode active sites. (C) 2021 Published by Elsevier B.V
Schematic synthesis of cobalt-oxide (Co3O4) supported cobalt-sulfide (CoS) composite for oxygen evolution reaction
No full textDevelopment of electrocatalysts has received great attention for storage and energy conversion technologies. Different electrocatalysts for oxygen evolution reaction (OER) have been produced and investigated but their role is not sufficient to mark of Ruthenium oxide (RuO2). Therefore, it is global requirement to produce an efficient, lower cost and earth-abundant electrocatalyst for OER. Herein, cobalt oxide-cobalt sulfide (Co3O4-CoS2) composite have been synthesized via hydrothermal chemical method with active performance OER. The desired overpotential is 280 mV to achieve current density of 20 mA cm(-2) of as prepared composite with Tafel slope value of 74 mV dec(-1). As prepared Co3O4-CoS2 composite has efficient stability of 30 hours for long term electrochemical performance
The Overexpression of Phasin and Regulator Genes Promoting the Synthesis of Polyhydroxybutyrate in Cupriavidus necator H16 under Nonstress Conditions
No full textCupriavidus necator H16 is an ideal strain for polyhydroxybutyrate (PHB) production from CO2. Low-oxygen stress can induce PHB synthesis in C. necator H16 while reducing bacterial growth under chemoautotrophic culture. The optimum growth and PHB synthesis of C. necator H16 cannot be achieved simultaneously, which restricts PHB production. The present study was initiated to address the issue through comparative transcriptome and gene function analysis. First, the comparative transcriptome of C. necator H16 chemoautotrophically cultured under low-oxygen stress and nonstress conditions was studied. Three types of genes were discovered to have differential levels of transcription: those involving PHB enzymatic synthesis, PHB granulation, and regulators. Under low-oxygen stress conditions, acetoacetyl-coenzyme A (CoA) reductase gene phaB2, PHB synthase gene phaC2, phasins genes phaP1 and phaP2, and regulator genes uspA and rpoN were upregulated 3.0-, 2.5-, 1.8-, 2.7-, 3.5-, and 1.6-fold, respectively. Second, the functions of upregulated genes and their applications in PHB synthesis were further studied. It was found that the overexpression of phaP1, phaP2, uspA, and rpoN can induce PHB synthesis under nonstress conditions, while phaB2 and phaC2 have no significant effect. Under the optimum conditions, the PHB percentage content in C. necator H16 was increased by 37.2%, 28.4%, 15.8%, and 41.0%, respectively, with overexpression of phaP1, phaP2, uspA, and rpoN, and the corresponding PHB production increased by 49.8%, 42.9%, 47.0%, and 77.5%, respectively, under nonstress chemoautotrophic conditions. Similar promotion by phaP1, phaP2, uspA, and rpoN was observed in heterotrophically cultured C. necator H16. The PHB percentage content and PHB production were increased by 54.4% and 103.1%, respectively, with the overexpression of rpoN under nonstress heterotrophic conditions.
IMPORTANCE Microbial fixation of CO2 is an effective way to reduce greenhouse gases. Some microbes, such as C. necator H16, usually accumulate PHB when they grow under stress. Low-oxygen stress can induce PHB synthesis when C. necator H16 is autotrophically cultured with CO2, H-2, and O-2, while under stress, growth is restricted, and total PHB yield is reduced. Achieving the optimal bacterial growth and PHB synthesis at the same time is an ideal condition for transforming CO2 into PHB by C. necator H16. The present study was initiated to clarify the molecular basis of low-oxygen stress promoting PHB accumulation and to realize the optimal PHB production by C. necator H16. Genes upregulated under nonstress conditions were identified through comparative transcriptome analysis and overexpression of phasin, and regulator genes were demonstrated to promote PHB synthesis in C. necator H16
