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    Ultrahigh-voltage integrated micro-supercapacitors with designable shapes and superior flexibility

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    With the development of power source-integrated electronics, the miniaturization of high-voltage integrated micro-supercapacitors (IMSCs) with multiple innovative form factors is urgently required but remains unsolved. Here, we demonstrate a universal, costeffective, industrially applicable protocol for fast and scalable fabrication of graphene-based planar IMSCs, with shape diversity, aesthetic versatility, outstanding flexibility and superior modularization. Using highly-conducting graphene ink, we directly screenprint shape-designable IMSCs in several seconds, consisting of hundreds to thousands of individual MSCs on arbitrary substrates. The resulting IMSCs are free of external metal current collectors and interconnects as well as separators, and exhibit exceptional electrical double-layer capacitive behaviors and remarkable flexibility. Notably, the output voltage and capacitance of IMSCs are readily adjustable through connection in well-defined arrangements of MSCs. As a proof of concept, a tandem energy storage pack of IMSCs with 130 MSCs can output a recorded voltage exceeding 100 V, demonstrative of superior modularization and performance uniformity

    National Natural Science Foundation of China[91326102]

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    煤炭清洁高效转化技术进展及发展趋势

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    我国石油、天然气资源匮乏,煤炭储量相对丰富,在未来相当长时间内,煤炭在能源消费结构中仍将占主导地位。煤炭清洁高效利用是能源生产与消费革命的主要内容,而煤炭清洁高效转化是煤炭清洁高效利用的重要途径,对保障国家能源安全、经济社会可持续发展意义重大。文章分析了国内外煤炭清洁高效转化技术的研究现状,以及未来我国煤炭高效转化的布局、研究方向和发展趋势,以期为提高我国煤炭清洁高效转化水平、加快推进我国能源消费革命提供技术支撑

    National Natural Science Foundation of China[21132004]

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    hierarchicalactivationofcompartmentalizedpoolsofampkdependsonseverityofnutrientorenergystress

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    AMPK, a master regulator of metabolic homeostasis, is activated by both AMP-dependent and AMP-independent mechanisms. The conditions under which these different mechanisms operate, and their biological implications are unclear. Here, we show that, depending on the degree of elevation of cellular AMP, distinct compartmentalized pools of AMPK are activated, phosphorylating different sets of targets. Low glucose activates AMPK exclusively through the AMP-independent, AXIN-based pathway in lysosomes to phosphorylate targets such as ACC1 and SREBP1c, exerting early anti-anabolic and pro-catabolic roles. Moderate increases in AMP expand this to activate cytosolic AMPK also in an AXIN-dependent manner. In contrast, high concentrations of AMP, arising from severe nutrient stress, activate all pools of AMPK independently of AXIN. Surprisingly, mitochondrion-localized AMPK is activated to phosphorylate ACC2 and mitochondrial fission factor (MFF) only during severe nutrient stress. Our findings reveal a spatiotemporal basis for hierarchical activation of different pools of AMPK during differing degrees of stress severity

    National Natural Science Foundation of China[91545202 and21703237]

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    China Postdoctoral Science foundation[2018M633455]

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    Identification and expression analysis of critical microRNA-transcription factor regulatory modules related to seed development and oil accumulation in developing Hippophae rhamnoides seeds

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    Sea buckthorn (Hippophae rhamnoides) seed oil is rich in omega fatty acids and various other bioactive compounds, which not only have high antioxidant activity but may also prevent cardiovascular diseases. However, the molecular mechanisms of oil biosynthesis and fatty acid accumulation in developing sea buckthorn seeds remain unknown. Altering the levels of certain miRNAs and transcription factors can affect the expression of multiple genes involved in seed development and fatty acid biosynthesis, resulting in changed seed oil contents. Considering that previous studies have focused mainly on these two regulators separately, we attempted to combine the study of miRNAs and transcription factors to construct a coregulatory network. In the present study, we generated cDNA libraries and small RNA libraries from the seeds of high-oil-content and low-oil-content sea buckthorn cultivars at four developmental stages, and then used high-throughput sequencing technology and bioinformatics methods to identify 140 differentially expressed transcription factors, 137 known miRNAs, and 250 novel miRNAs. Through target relationship prediction, 31 potential transcription factors were found to be associated with 27 miRNAs in 18 independent networks, showing the multiplicity of the regulators. In particular, 9 miRNAs and 5 transcription factors were found as hubs in the network. Furthermore, we hypothesized that 12 regulatory modules might play combinatory regulatory roles in seed development and oil accumulation. In developing seeds, the expression levels of these miRNAs and transcription factors were significantly different between the two cultivars. Taken together, these data describe the first miRNA-transcription factor coregulatory network in developing seeds of sea buckthorn. These findings will be helpful for understanding seed development and oil accumulation at the molecular level in sea buckthorn

    Identification and expression analysis of critical microRNA-transcription factor regulatory modules related to seed development and oil accumulation in developing Hippophae rhamnoides seeds

    No full text
    Sea buckthorn (Hippophae rhamnoides) seed oil is rich in omega fatty acids and various other bioactive compounds, which not only have high antioxidant activity but may also prevent cardiovascular diseases. However, the molecular mechanisms of oil biosynthesis and fatty acid accumulation in developing sea buckthorn seeds remain unknown. Altering the levels of certain miRNAs and transcription factors can affect the expression of multiple genes involved in seed development and fatty acid biosynthesis, resulting in changed seed oil contents. Considering that previous studies have focused mainly on these two regulators separately, we attempted to combine the study of miRNAs and transcription factors to construct a coregulatory network. In the present study, we generated cDNA libraries and small RNA libraries from the seeds of high-oil-content and low-oil-content sea buckthorn cultivars at four developmental stages, and then used high-throughput sequencing technology and bioinformatics methods to identify 140 differentially expressed transcription factors, 137 known miRNAs, and 250 novel miRNAs. Through target relationship prediction, 31 potential transcription factors were found to be associated with 27 miRNAs in 18 independent networks, showing the multiplicity of the regulators. In particular, 9 miRNAs and 5 transcription factors were found as hubs in the network. Furthermore, we hypothesized that 12 regulatory modules might play combinatory regulatory roles in seed development and oil accumulation. In developing seeds, the expression levels of these miRNAs and transcription factors were significantly different between the two cultivars. Taken together, these data describe the first miRNA-transcription factor coregulatory network in developing seeds of sea buckthorn. These findings will be helpful for understanding seed development and oil accumulation at the molecular level in sea buckthorn

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