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Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization
No full textNine transformants of Rhodosporidium toruloides with significant changes in the carotenoid profile were obtained by Agrobacterium tumefaciens-mediated transformation, including a white, three red, and four yellow mutants. A red mutant Al-15-BRQ that showed a high torularhodin production was selected for culture condition optimization. Results indicated that the torularhodin yield was boosted with glucose as the carbon source, at a carbon/nitrogen ratio of 22, a loading volume of 75 mL, and 28 degrees C. The torularhodin yield of 21.3 mg/L consisting of 94.4% total carotenoids was obtained by Box-Behnken design experiments. The torularhodin yield was 17.0-fold higher than that of the wild type, with time shortened from 9 to 3 days. This study reports an effective strategy for improving torularhodin production and provides a candidate R toruloides strain for highly selective production of torularhodin
Tuning the functionalization degree of graphene: Determining critical conditions for inhibiting the corrosion promotion activity of graphene/epoxy nanocomposite coatings
No full textGraphene functionalization helps to improve the barrier property of graphene-reinforced coatings, however, it may also increase the electrical conductivity of coatings and result in galvanic corrosion between coating and metal. To demonstrate that fine-tuned functionalization of graphene is vital for its applications in metal protection, reduced graphene oxide (rGO) with various functionalization degree was precisely prepared. Compared with pristine rGO, rGO with a low functionalization degree possesses an enhanced corrosion promotion activity (CPA), and rGO with a high functionalization degree exhibits an inhibited CPA. Critical functionalization degree for inhibiting the CPA of rGO/epoxy nanocomposite coatings was determined. (C) 2019 Elsevier B.V. All rights reserved
