idUS. Depósito de Investigación Universidad de Sevilla
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Pablo de Rokha's Epopeya de las comidas y las bebidas de Chile: a defence of culinary tradition at the beginning of globalization
No full textInclusive policies in performing arts: a case study of Chile’s approach to disability
No full textThis study investigates the impact of cultural policies on performing
arts attendance among individuals with disabilities in Chile,
highlighting how accessibility, sociodemographic factors, and
institutional practices shape cultural participation. Drawing on
data from the 2017 National Survey of Cultural Participation in
Chile (N = 12,151), this research employs quantitative analysis to
explore barriers faced by people with disabilities. Our findings
underscore the limited efficacy of current policies in promoting
inclusivity within the performing arts sector in Chile. We argue
that without targeted policy reforms aimed at improving
accessibility and fostering inclusivity, cultural institutions will
continue to marginalize individuals with disabilities. The study
contributes to the discourse on inclusive policy, advocating for
systemic changes to create equitable opportunities for cultural
engagement and strengthen the commitment to diversity and
inclusion within Chile’s cultural landscape
Assessment of carbon dioxide transcritical cycles for electrothermal energy storage with geological storage in salt cavities
No full textCarbon capture, utilisation, and storage (CCUS) technologies are envisaged as critical actors in the energy transition and climate change mitigation framework. Despite the recent advances in CCUS implementation, there is still significant scope for their growth and improvement. On the other hand, developing new large-scale energy storage systems is a key factor for the massive deployment of renewable energy systems. Technologies such as the electrothermal energy storage system based on carbon dioxide transcritical cycles incorporating geological storage (CEEGS) can contribute to both fields. Preliminary studies have shown that the integrated storage system can operate with roundtrip efficiencies exceeding 50%, injecting over 1 million tons of captured carbon dioxide (CO2) annually. It is an early-stage technology with open challenges for successful development, such as an adequate balance between the high- and low-temperature thermal storage reservoirs or the operation definition between the electrothermal system and the carbon dioxide injection and recovery. Underground pressures will oscillate with the charge/discharge process, with operational implications and constraints. Advancing in the concept requires the definition of optimised operation. This work presents a novel analysis of the adaptation of CO2 transcritical cycles to include injection and production processes with the specific scenario of salt cavities. It considers new modifications to adapt the transcritical cycles in closed-mode operation to the conditions required by the salt cavities injection and production processes. Different case studies are evaluated, evaluating the impact of injection and geological storage, production and surface storage, and reinjection into the geological formation. The analyses show roundtrip efficiencies within the range of 49.1–73.0% when the injection, production, and reinjection processes are executed
Research paper's dataset: “The nutrient status of halophytes demonstrates a species-specific pattern, which depends more on microorganisms and enzymal activities than on the physical-chemical characteristics of the soil”
No full textThis experiment is part of the project "Analysis of the contribution of endophytic bacteria to the tolerance mechanisms of halophytes against salinity and climate change (PID2021-124750NB-I00)". This specific work analyzes the nutritional content of halophytes of different functional types (three hydrohalophytes and three xerohalophytes), the physicochemical and nutrient characteristics of the soil where they grow, and the enzymatic activities and fatty acid content of the soil.Este experimento se engloba en el proyecto “Análisis de la contribución de las bacteriaas endófitas a los mecanismos de tolerancia de las halófitas frente a la salinidad y el cambio climático (PID2021-124750NB-I00)”. En este trabajo concreto se analiza el contenido nutricional de halófitas de distinto tipo funcional (tres hidrohalófitas y tres xerohalófitas), las características físico-químicas y nutrientes del suelo donde crecen, y las actividades enzimáticas y contenido de ácidos grasos del suelo
Efecto del verbascósido en la producción de mediadores inflamatorios en células SW982
No full textIntroducción: La artritis reumatoide (AR) es una enfermedad inflamatoria inmunomediada decarácter crónico y con afectación sistémica, que afecta la cavidad sinovial de las articulaciones, provocando hiperplasia, sinovitis y destrucción progresiva del cartílago. En este proceso, los fibroblastos sinoviales (FS) adquieren un fenotipo alterado que favorece la producción de mediadores proinflamatorios y de degradación tisular, además de contribuir a la formación del pannus. El verbascósido (VB), un compuesto fenólico presente en plantas como Olea europaea, ha mostrado ejercer importantes propiedades antioxidantes y antiinflamatorias en estudios previos, aunque no se han investigado aún sus efectos antirreumáticos ni los mecanismos moleculares y vías de señalización involucradas en FS humanos.
Objetivo: Examinar la capacidad antiinflamatoria del VB en células sinoviales humanas SW982, tras su estimulación con interleucina (IL)-1β, mediante la evaluación de la generación de mediadores inflamatorios y el estudio de las vías de señalización relacionadas.
Metodología: Las células SW982 se cultivaron en DMEM y se estimularon con IL-1β, en presencia o ausencia de VB. Se evaluó la viabilidad celular mediante el test de sulforodamina B (SRB), y los niveles de producción de citocinas proinflamatorias TNF-α e IL-6 por ELISA. Además, se analizó la expresión proteica de las enzimas proinflamatorias COX-2 y mPGES-1, junto con la vía de señalización celular antioxidante Nrf2/HO-1 mediante Western blot.
Resultados: El tratamiento con VB redujo significativamente los niveles de las citocinas por- inflamatorias IL-6 y TNF-α y la expresión proteica de la enzima COX-2. Por el contrario, no se obtuvieron resultados significativos sobre la expresión proteica de m-PGES-1 ni de la vía antioxidante Nrf2/HO-1 a las concentraciones ensayadas.
Conclusión: El VB podría integrarse en las nuevas estrategias terapéuticas para tratar y/o prevenir la inflamación articular. Sin embargo, aun faltan estudios in vivo que validen el posible potencial antirreumático de este compuesto fenólico.Introduction: Rheumatoid arthritis (RA), a chronic immune inflammatory disease that affects the synovial cavity of the joints, leading to hyperplasia, synovitis, and progressive cartilage destruction. In this process, synovial fibroblasts (SF) acquire an altered phenotype that promotes the production of inflammatory mediators and tissue degradation, as well as contributing to the formation of pannus. Verbascoside (VB), a phenolic compound found in plants such as Olea europaea, has shown antioxidant and anti-inflammatory properties in previous studies; however, its anti-rheumatic effects and the molecular mechanisms and signaling pathways involved in human SF have not been investigated yet.
Objective: to examine the anti-inflammatory capacity of VB in human synovial SW982 cells after stimulation with IL-1β, by evaluating the generation of inflammatory mediators and studying the related signaling pathways involved.
Methodology: SW982 cells were cultured in DMEM and stimulated with IL-1β, in presence or absence of VB. Cell viability was assessed using sulforhodamine B (SRB) test, and TNF-α and IL-6 production levels were measured by ELISA. Additionally, the expression of inflammatory proteins COX-2 and mPGES-1, and the role of Nrf2/HO signaling pathway were analysed by Western blot.
Results: Treatment with VB significantly reduced levels of both pro-inflammatory cytokines IL-6 and TNF-α as well as the protein expression of COX-2 enzyme. However, no significant effects were observed on mPGES-1 and the antioxidant Nrf2/HO-1 pathway after VB treatment at doses assayed.
Conclusion: VB could be incorporated into new therapeutic strategies to treat and/or prevent joint inflammation. However, in vivo studies are needed to validate the potential anti-rheumatic effects of this phenolic compound.Máster Universitario en Especialización Profesional en Farmaci
Experimental data on sequence variability in Salmonella
No full textThis dataset was generated as a part of a study investigating the mechanisms of bacterial adaptation, with particular emphasis on sequence variability within promoter regions of surface-related genes in Salmonella. The work focused on the opvAB operon, whose transcription is epigenetically regulated through Dam-mediated methylation of GATCs sites and OxyR binding, and plays a key role in Salmonella adaptation under selective pressures such as bacteriophages presence. The dataset, which combines bioinformatic analyses with laboratory experiments,
is organized into several files:
• BLASTn results: .xlsx file containing alignment information obtained from BLASTn searches using the regulatory sequences (RS) and coding sequences (CDS) of surface-related and housekeeping genes from Salmonella.
• G+C content analysis: .dna file containing the sequence of the opvAB operon along with 1.5 kb of upstream and downstream flanking regions. This file enables the identification of the opvAB-containing sequence potentially acquired through horizontal gene transfer by assessing differences in G+C content when compared to the characteristic composition of the Salmonella genome.
• Prevalence of opvAB in the genus Salmonella: .xlsx file quantifying the presence of the opvAB operon in Salmonella genomes available in the NCBI database, organized by species (green) and subspecies (purple).
• Analysis of regulatory elements diversity across Salmonella enterica subspecies: .xlsx files detailing the conservation of the opvAB promoter's regulatory elements (-35 and -10 boxes, four GATCs and four OxyR Binding Sites [OBSs]) across the enterica, arizonae, diarizonae and salamae subspecies.
• Nucleotide composition of OBSs across Salmonella enterica subspecies: .xlsx file showing the percentage of adenine (A), guanine (G), cytosine (C), and thymine (T) nucleotides for each OxyR binding site (OBSs) across the enterica, arizonae, diarizonae and salamae subspecies.
• Analysis of mutational distribution within the opvAB regulatory region: .xlsx file reporting the location of mutations in the opvAB promoter across Salmonella enterica subsp. enterica genomes containing up to eight mutations, as well as in environmental strains isolated from poultry farms. Sequence variations are annotated using a numerical code: 0=conserved position, 1=substitution, 2=insertion, and 3=deletion.
• Flow Cytometry Data: .lmd files and an associated .xlsx file showing the percentage of cells expressing the opvAB operon (OpvABON).File name: BLASTn results.xlsx
Description: .xlsx file containing alignment information obtained from BLASTn searches using the regulatory sequences (RS) and coding sequences (CDS) of surface-related and housekeeping genes from Salmonella.
File name: G+C content analysis.dna
Description: .dna file containing the sequence of the opvAB operon along with 1.5 kb of upstream and downstream flanking regions.
File name: Prevalence of opvAB in the genus Salmonella.xlsx
Description: .xlsx file quantifying the presence of the opvAB operon in Salmonella genomes available in the NCBI database, organized by species (green) and subspecies (purple).
File name: Analysis of regulatory elements diversity across Salmonella enterica subspecies.xlsx
Description: .xlsx files detailing the conservation of the opvAB promoter's regulatory elements (-35 and -10 boxes, four GATCs and four OxyR Binding Sites [OBSs]) across the enterica, arizonae, diarizonae and salamae subspecies.
File name: Nucleotide composition of OBSs across Salmonella enterica subspecies.xlsx
Description: .xlsx file showing the percentage of adenine (A), guanine (G), cytosine (C), and thymine (T) nucleotides for each OxyR binding site (OBSs) across the enterica, arizonae, diarizonae and salamae subspecies.
File name: Analysis of mutational distribution within the opvAB regulatory region.xlsx
Description: .xlsx file reporting the location of mutations in the opvAB promoter across Salmonella enterica subsp. enterica genomes containing up to eight mutations, as well as in environmental strains isolated from poultry farms. Sequence variations are annotated using a numerical code: 0=conserved position, 1=substitution, 2=insertion, and 3=deletion.
File name: Percentage of OpvAB-ON cells.xlsx
Description: .xlsx file showing the percentage of cells expressing the opvAB operon (OpvABON) obtained from different replicates.
File name: Mut. OBS_A.LMD
Description: Flow cytometry raw file of the ATCC 14028 strain carrying a mutation in OxyR Binding Site A with an opvB::gfp transcriptional fusion.
ile name: Mut. OBS_B.LMD
Description: Flow cytometry raw file of the ATCC 14028 strain carrying a mutation in OxyR Binding Site B with an opvB::gfp transcriptional fusion.
File name: Mut. OBS_C.LMD
Description: Flow cytometry raw file of the ATCC 14028 strain carrying a mutation in OxyR Binding Site C with an opvB::gfp transcriptional fusion.
File name: Mut. OBS_D.LMD
Description: Flow cytometry raw file of the ATCC 14028 strain carrying a mutation in OxyR Binding Site D with an opvB::gfp transcriptional fusion.
File name: Wild type_no GFP.LMD
Description: Flow cytometry raw file of the ATCC 14028 wild-type strain without a GFP reporter.
File name: opvB_gfp.LMD
Description: Flow cytometry raw file of the ATCC 14028 wild-type strain carrying the opvB::gfp transcriptional fusion.
File name: SV10095.LMD
Description: Flow cytometry raw file of the environmental Salmonella isolate SV10101 carrying opvB::gfp transcriptional fusion.
File name: SV10096.LMD
Description: Flow cytometry raw file of the environmental Salmonella isolate SV10192 carrying opvB::gfp transcriptional fusion.
File name: SV10097.LMD
Description: Flow cytometry raw file of the environmental Salmonella isolate SV10193 carrying opvB::gfp transcriptional fusion.
File name: SV10098.LMD
Description: Flow cytometry raw file of the environmental Salmonella isolate SV10194 carrying opvB::gfp transcriptional fusion.
File name: SV10099.LMD
Description: Flow cytometry raw file of the environmental Salmonella isolate SV10195 carrying opvB::gfp transcriptional fusion
Size tuneability of highly efficient li-rich cathode materials using an emulsion-based synthesis route
No full textLithium- and manganese-rich transition metal oxides exhibit excellent specific capacities, making them strong candidates for the development of the next generation of Co-free lithium-ion batteries. In this study, the synthesis of size-tunable Li₁ꓸ₂Ni₀ꓸ₂Mn₀ꓸ₆O₂ using a synthetic route based on the formation of an emulsion, which is ultra-fast, cost-effective, and easily scalable to an industrial level is presented. We demonstrate that variations in the concentrations of hydrophobic, hydrophilic, and surfactant components, which lead to micelle formation within the emulsion, have a significant impact on the average particle size and size distribution of the synthesized material, and subsequently, on their electrochemical performance. Specifically, increasing the concentration of oleic acid as a surfactant results in an optimal average particle size, with discharge specific capacities exceeding 317 mAh g⁻¹ in the first cycle and 230 mAh g⁻¹ after 100 cycles, demonstrating an excellent battery performance comprising state-of-the-art lithium- and manganese-rich transition metal oxide materials
Verification and validation of the horseradish peroxidase (HRP)-mediated enzymatic cross-linking in collagen/PEG hydrogels for wound dressing applications
No full textCollagen/poly(ethylene glycol) (COL/PEG) hydrogels are attractive wound dressings but typically rely on weak hydrogen bonding at the polymer interface. Here, a catechol–enzymatic strategy is introduced in which dopamine (DA) self-polymerization reduces graphene oxide to conductive rGO while horseradish peroxidase/hydrogen peroxide (HRP/H2O2) catalyzes covalent coupling between native PEG hydroxyls and collagen phenolic residues. Spectroscopic and thermo-mechanical signatures, attenuation of –OH/amide bands in FTIR, a glass-transition shift from −9 °C to −4 °C, increased storage modulus with elastic dominance (E′ ≫ E″, tan δ 99 % reduction of E. coli and ≥90 % of S. aureus), sustained antioxidant capacity, and cytocompatibility (≥80 % viability in human dermal fibroblasts and keratinocytes) with negligible hemolysis (<1 %). In vitro scratch assays reached 100 % closure by 48 h. In a porcine full-thickness wound model, HRP-cross-linked, rGO-reinforced COL/PEG hydrogels achieved accelerated re-epithelialization by day 21, robust angiogenesis and granulation, and no detectable irritation or systemic pathology. These results establish HRP-mediated, DA-assisted cross-linking as a minimally modified route to conductive, antioxidant COL/PEG hydrogels with tunable mechanics and clinically relevant regenerative efficacy
Enhancing Fundamental Motor Skills Through Active Play: A Systematic Review and Meta-Analysis of Educational Contexts
No full textThis study examines the effectiveness of active play interventions to enhance Fundamental
Motor Skills (FMS) in children aged 2 to 5 and compares different intervention modalities. It
also explores the moderating role of world region and the use of recess in educational contexts. A systematic review and Bayesian meta-analysis were conducted, using data from MEDLINE, Web of Science (WOS), Scopus, PsycINFO, Cinahl, and SPORTdiscus up to January 2023. Clinical trials with active play interventions in educational settings were included, excluding multicomponent interventions. Eighteen studies (2816 participants; 240 effect sizes) met the criteria. The overall average treatment effect (ATE) of active play versus control was 0.07 (95% CrI -0.004 to 0.15). Motor skills interventions showed a significant effect (SMD = 0.16, 95% CrI 0.01 to 0.29), especially when integrated into recess (SMD =0.31, 95% CrI 0.03 to 0.62). The effectiveness varied across regions, highlighting the need for regional consideration in implementatio
Los continos reales de Castilla: entre el oficio y el servicio a la monarquía (1474-1520)
No full textLa colaboración activa de los continos reales en todas las empresas de la monarquía: Canarias, Granada, América, así como en los ámbitos de competencia regia (justicia, hacienda, ejército, control del territorio o relaciones diplomáticas con otras monarquías extranjeras) muestra la trascendencia que desempeñaron como instrumentos de gobierno y de acción política. Dado su origen mayoritariamente urbano, la estrecha colaboración de los continos con la Corona pone de relevancia el papel de las ciudades en el gobierno castellano. Desde la perspectiva de la historia social, este estudio permite profundizar en el valor que el cargo tuvo para aquellos que lo ejercieron y en el papel que desempeñó dentro de las estrategias familiares de las oligarquías urbanas en Castilla entre 1474 y 1520, considerado el período de mayor actividad de estos servidores regios