Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Not a member yet
145 research outputs found
Sort by
ANALYSIS OF INTERLEUKIN-10 LEVELS IN MESENCHYMAL STEM CELL SECRETOME CREAM WITH ELISA METHOD
Full text linkMesenchymal Stem Cells (MSC) are cells that are able to differentiate into other cells and have immunomodulatory properties that are used to treat inflammatory diseases. MSCs have the ability to repair damaged tissue by regenerating damaged tissue. MSCs produce a secretome, where the MSC secretome has various cytokines, chemokines, growth factors, anti-inflammatory factors and proteins that are produced in molecular form. One of the molecules secreted by the secretome is Interleukin-10.IL-10 is an anti-inflammatory cytokine which functions to inhibit the production of several other types of cytokines such as TNF, IL-1, chemokine and IL-12). Apart from that, IL-10 is able to inhibit the function of macrophages in assisting T cell activity. One of the products from Secretome MSC is a cream preparation. To find out whether the MSC cream contains IL-10 protein, Interleukin-10 levels were analyzed in the MSC secretome cream with 3 different MSC cream concentration formulations (2%, 3% and 5%). Analysis of IL-10 levels was carried out using ELISA to detect IL-10 antigen. The results of the examination of 9 samples showed that all samples were positive for IL-10 with a concentration of 2% dilution 1:2 showing the best results with an Optical Density (OD) of 0.069 and followed by a concentration of 5% dilution 1:2 showing an OD value of 0.065
THE PATHOPHYSIOLOGY OF CUTANEOUS MANIFESTATIONS RELATED TO COVID-19
Full text linkCovid-19 is a respiratory infection caused by the newly emerging coronavirus. Covid-19 can also cause skin abnormalities, although the pathophysiology of manifestations of skin disorders associated with Covid-19 has not been understood with certainty. This study aims to discuss the pathophysiology of cutaneous manifestation related to Covid-19. The study was conducted based on literature and data mining related to Covid-19 using PubMed and Google Scholar. Eligible papers provided pathophysiology of cutaneous manifestations related to Covid-19. Papers not available in English were excluded. As a result of the presence of edema, vasodilation, and cellular infiltration, skin lesions such as rash, urticarial, or extensive purpura, or even thrombosis and haemorrhage, arise if damage to the vessels became severe. The pathophysiology of Covid-19-associated skin manifestations is estimated through some different mechanisms, such as lesions found in various Covid-19 patients, namely, the presence of varicella such as eruption, non-characteristic rash, and urticarial. Further studies are required to establish a Covid-19 diagnosis based on skin manifestations
ANTIDIABETIC ACTIVITY OF LEAF EXTRACT OF Clerodenrum fragrans Vent Willd IN Rattus novergicus INDUCED BY ALLOXAN
Full text linkSarang banua plants are grown in Simalungun, North Sumatra, Indonesia, and have been used by the community as traditional medicinal plants. Sarang banua plant is a type of Clerodendrum fragrans Vent Willd, including the family Verbenaceae. This study aimed to determine the antidiabetic activity of the leaf extract of sarang banua (C. fragrans Vent Willd) in white male rats (Rattus novergicus) induced by alloxan. This study used a RAL design with seven treatments, namely (K0) standard feed, (K1) Na-CMC 0.5%), (K2) metformin, (K3) ethanol extract 100 mg/kg bw, 200 mg/kg bw (K4), 300 mg/kg bw (5), (K6) ethyl acetate extract 200 mg/kg bw and 300 mg/kg bw (K7). Groups K1 to K7 were induced by alloxan before being given treatment. Each treatment was replicated three times. The results showed that the application of leaf extract of the C. fragrans affected on reducing the blood glucose levels of alloxan-induced rats. The used of ethanolic extract of C. fragrans 100 mg/kg bw resulted in the highest percentage decrease in blood glucose (54.46 ± 5.60%) of hyperglycemic rats induced by alloxan, close to a positive control (56.63 ± 1.86%). 
THE EFFECT OF INCUBATION PERIOD ON ETHANOL CONTENT OF JASMINE AND GREEN TEA KOMBUCHA
Full text linkKombucha is a fermented tea that provides many benefits for health. The alcohol (ethanol) content of kombucha has withdrawn a lot of attention because of human health risks and halal concerns. Alcohol and Tax and Trade Bureau (TTB), BC Center for Disease, and MUI fatwa have defined that kombucha is safe to be consumed as long as the alcohol content is less than 0.5%. This study was conducted to compare the alcohol (ethanol) concentration of jasmine and green tea kombucha during the fermentative stage (5 day at 25oC) and post-fermentative stage (7 and 21 day, both at 4oC). The result showed that ethanol concentrations for jasmine and green tea kombucha rose from the fifth d of incubation to the seventh d of the post-fermentative stage, followed by a decline at 21th d of the post-fermentative stage (p>0.05). The level of organic acids, Acetic Acid Bacteria (AAB), and pH confirmed that there were no significant differences at all (p>0.05). In conclusion, jasmine and green tea kombucha at fermentative stage (5 d at 25oC) and post-fermentative stage (7 and 21 day, both at 4oC) did not produce ethanol more than 0.5%, without any significant differences in organic acids and AAB levels, respectively
DETERMINATION OF OPTIMAL ANNEALING TEMPERATURE Vibrio alginolyticus PRIMERS USING POLYMERASE CHAIN REACTION METHOD
Full text linkFood poisoning is a global issue of grave concern. If food is not properly cooked, it can be a medium for the spread of pathogenic bacteria. Vibrio alginolyticus is one of the pathogenic bacteria that can cause food poisoning. real-time Polymerase Chain Reaction (rt-PCR) can detect pathogenic bacteria in food, so it is necessary to determine the optimal annealing temperature. This research aims to obtain the optimal annealing temperature of the Va_Chr1_FR primer using Gradient PCR. The DNA concentration used was 174.5 with an A260/A280 purity of 1.94. The temperature range tested, 53°C-62°C, corresponds to the melting temperature of the Va_Chr1_FR primers. The primers designed were F5'-TTCTTCTGTTGTAGGTTCCG-F3' and R5'-CCAGCCCTCACATCTAATAC-R3'. Based on these results, a temperature of 60°C is deemed as the most optimal annealing temperature because it produces one of the brightest bands on electrophoresis with an amplicon length of 146 bp. The findings of this study will be beneficial to the development of Va_Chr1_FR Vibrio alginolyticus primers testing on food samples using the real-time PCR method.