Archives of Agriculture and Environmental Science
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Purification of biodiesel-derived crude glycerol and its application in plasticizing cassava starch bioplastics
No full textThis study focused on purifying crude glycerol, a by-product of biodiesel production, using acid-precipitation, methanol extraction, and adsorption with acid-activated charcoal. Among the acids tested—sulfuric, phosphoric, and hydrochloric—phosphoric acid proved most effective, producing the clearest glycerol with minimal salt deposition. The purified glycerol was then used to produce bioplastics, which were tested for mechanical properties. The results indicated that Phosphoric acid yielded the clearest glycerol with minimal salt deposition. The resultant salt, potassium phosphate (K2PO4), has potential as a fertilizer. The purified glycerol showed increased density and viscosity, indicating higher purity compared to crude glycerol. The density of the purified glycerol was closer to that of analytical-grade glycerol. Bioplastic 1 (using analytical-grade glycerol) exhibited the highest tensile strength, withstanding up to 4.3N and extending about 104mm before breaking. Bioplastic 2 (using glycerol purified with hydrochloric acid) withstood up to 4.1N, while Bioplastic 3 (using glycerol purified with acetic acid) endured the least stress, withstanding up to 3.8N and extending up to 87mm before breaking. The study demonstrates that phosphoric acid is an effective agent for purifying crude glycerol, significantly enhancing its quality. The purified glycerol, in turn, improves the mechanical properties of bioplastics, making them more durable and suitable for a range of applications. This process not only adds value to the biodiesel production by-product but also contributes to the development of stronger, more versatile bioplastics
Assessment of onion farming practices and purple blotch disease knowledge among farmers in varied agro-ecological zones of Nyeri County, Kenya
No full textOnion (Allium cepa L.) is the second most produced vegetable globally, following tomato and plays a vital role in both cuisines and daily diets. However, the threat of diseases, such as purple blotch caused by Alternaria porri, poses a substantial risk to onion production, particularly in Nyeri County, Kenya. Despite its critical impact on farming, there is a lack of information on farmers' knowledge of purple blotch in this region. This study aimed to assess the onion farming practices and farmers' understanding of purple blotch disease across various agro-ecological zones (AEZs) in Nyeri County. Specifically, the study examines farmers' demographics, cultivated onion varieties, and their knowledge of purple blotch disease. Farms were selected using cluster random sampling. Data were collected from 100 onion farmers through semi-structured questionnaires, and statistical analysis was performed using the chi-square test in Scientific Analysis System (SAS) version 9.4 at α=0.05. The findings revealed that while the Rucet F1 onion variety was popular among the farmers (52%), there is no significant association (X2 (6, 100) = 11.947, p = 0.063) between the choice of variety and AEZs. Similarly, the preferred source of onion seeds, mainly Agroshop (84%), showed insignificant association (X2 (9, 100) = 7.153, p = 0.621) with AEZs. Despite 65% of farmers reporting knowledge about onion diseases, there is no significant association (p > 0.05) between their awareness of purple blotch and AEZs. In conclusion, the study highlights a significant gap in farmers' understanding of purple blotch disease, emphasizing the need for training programs to enhance disease identification skills. Early detection can empower farmers to implement proactive measures, ultimately improving onion productivity. This study recommends diversifying onion varieties for disease resilience, promoting awareness and training on purple blotch identification, engaging women and youths in farming, and fostering collaborative networks for ongoing knowledge exchange and improvement in onion cultivation in Nyeri County
Length frequency distribution, length-weight relationship and condition factors of Hilsa Shad, Tenualosa ilisha from Bangladesh
No full textHilsa shad, Tenualosa ilisha, a cherished and economically vital species, thrives in the waters of Bangladesh. It holds a prominent role in bolstering the nation's food security and influencing the ecological dynamics of aquatic ecosystems along the Bangladeshi coast and estuaries. This comprehensive study encompasses data collection efforts on the Hilsa fish species, conducted at 12 commercial landing centers throughout Bangladesh over the course of 2020. The study describes some fundamental components, including length frequency distribution, length-weight relationships, and condition factors, offers invaluable insights into the growth, health, and overall well-being of Hilsa population. Notably, the length-weight relationship analysis revealed size ranges of 11.5-59.2 cm and 11.0-49.0 cm total length for females and males, respectively. The observed b values indicated positive allometric growth (b > 3.00) for female population and negative allometric growth (b < 3.00) for male population in the length-weight relationship. Among the condition factors scrutinized, Fulton's condition factor emerged as the most reliable indicator for assessing the health and condition of the Hilsa population. This study offers valuable insights for biologists, aiding in the assessment of the species' status and providing essential information for fishery biologists and conservation biologists. Such insights are instrumental in the management and ultimate conservation of this iconic species in Bangladesh
An ideal model of plant-vector-phytopathogen interaction and the management of the vector
No full textMaize (Zea mays L.) is a vital cereal crop worldwide, crucial for global food security and various industrial applications. Its cultivation faces significant challenges from a diverse array of insect pests and pathogens, notably including the maize leaf aphid (Rhopalosiphum maidis) and the maize dwarf mosaic virus (MDMV). This paper explores the intricate interactions among maize, its insect vectors, and MDMV, emphasizing the urgent need for a deeper understanding to develop sustainable management strategies. Maize exhibits vast genetic diversity and is cultivated across diverse environments, making it susceptible to a range of pests and diseases. The transmission of MDMV by aphids, particularly R. maidis, poses a significant threat to maize production globally. The complex tripartite interaction between maize, aphids, and MDMV serves as an ideal model system for studying plant-insect-phytopathogen interactions. Understanding the components of this interaction is critical for developing effective management strategies. Despite significant research efforts, there remains a knowledge gap in the molecular mechanisms underlying vector-borne diseases. Further research on the molecular level is essential for identifying specific targets for genetic pest control and disrupting pathogen transmission by insect vectors. Developing countries, in particular, require intensified research efforts to address the growing challenges to food security and agricultural sustainability. Thus, unraveling the complexities of plant-insect-phytopathogen interactions is essential for devising effective strategies to combat vector-borne diseases and sustain global food systems. Enhanced scientific research, especially in developing regions, is crucial for addressing these challenges and ensuring food security for future generations
Effect of boron and molybdenum on growth and yield attributes of cauliflower (Brassica oleraceae Var. Botrytis L) at Salyan, Nepal
No full textA research study was conducted to investigate the impact of varying levels of boron and molybdenum on the growth and yield parameters of the Silvercup-60 variety of cauliflower in the fields of Luham, Salyan during the winter season of 2022. The experiment comprised seven treatments arranged in a Randomized Complete Block Design (RCBD) with three replications. Each replication included seven treatments denoted as follows: T1 (Control), T2 (Borax @10 kg/ha), T3 (Ammonium Molybdate @1 kg/ha), T4 (Borax @10 kg/ha + Ammonium Molybdate @1 kg/ha), T5 (Borax @10 kg + Ammonium Molybdate @2 kg/ha), T6 (Borax @20 kg/ha + Ammonium Molybdate @1 kg/ha), and T7 (Borax @20 kg/ha + Ammonium Molybdate @2 kg/ha), representing different doses of boron and molybdenum. Various growth parameters, including plant height, number of leaves, leaf length, and leaf width, were recorded at intervals of 15, 30, 45, and 55 days post-transplanting, along with yield parameters such as curd diameter and curd yield. Notably, treatment T4 (Borax @10 kg/ha + Ammonium Molybdate @1 kg/ha) exhibited significantly superior curd diameter (19.03 cm) and yield (16.41 mt/ha) compared to the control group, while the control group yielded the lowest values for these parameters. Based on the findings of this study, it can be concluded that the application of boron and molybdenum at a rate of 10 kg/ha of Borax and 1 kg/ha of Ammonium Molybdenum is recommended for cauliflower cultivation in the Salyan district, as it leads to enhanced growth and yield of cauliflower crops
Insights into the germplasm conservation and utilization: Implications for sustainable agriculture and future crop improvement
No full textPlant genetic resources are critical for maintaining global biodiversity and ensuring food security. However, these resources face threats from factors such as habitat loss and climate change, with approximately 22% of plant species estimated to be at risk of extinction. To address this issue, both natural and biotechnological methods are being developed to preserve plant genetic resources, with germplasm being a key component. Germplasm contains the complete genetic information of a plant and can be stored for extended periods and replicated as required. The objective of this study is to emphasize the importance of preserving germplasm of endangered or near-extinct plant species through in situ and ex situ conservation methods. In situ conservation involves conserving species in their natural environment, while ex situ conservation includes using gene-seed banks and tissue culture to store genetic resources. These methods are crucial for maintaining genetic diversity and preventing the loss of valuable plant resources. The study highlights the various ex situ conservation methods, including cryopreservation, pollen and DNA banks, farmer's fields, botanic gardens, genetic reserves, and slow-growing cultures, which are essential for preserving germplasm. Gene banks worldwide currently hold over 7.4 million accessions of crop genetic resources, demonstrating the value of germplasm conservation efforts. Additionally, understanding the phenotypic and genetic characterization of related species is crucial for identifying endangered or vulnerable species that can diversify into new varieties or subspecies. In conclusion, prioritizing germplasm conservation efforts is crucial for meeting future demands while preserving endangered or vulnerable species. This will ensure that plant genetic resources remain available for future generations and that agricultural innovation can effectively address global food security challenges
Impact of IPM training on pest management practices in major vegetables in Palpa, Nepal
No full textA study assessed the impact of Integrated Pest Management (IPM) training on pest management practices in major vegetable crops in Palpa district. A total of 138 respondents were selected through purposive random sampling from Tansen municipality and the rural municipalities of Bagnaskali and Ribdikot. Data was collected via semi-structured interviews on prevailing IPM practices, pesticide handling, and challenges in IPM adoption. The analysis, employing descriptive and inferential statistics including chi-square tests, revealed that most trained respondents were from Tansen municipality, with more females than males receiving training. Although respondents preferred botanical methods, they predominantly used chemical pesticides due to availability, lack of biopesticides, high costs of IPM, social constraints, and the absence of block farming. Agro-vet stores were the main information source on pesticides. The level of pest control influenced chemical pesticide purchases, with low awareness of pest resistance. Both trained and non-trained respondents sprayed pesticides during pest outbreaks, with few reporting symptoms from exposure. Trained respondents exhibited greater awareness of the impacts of chemical pesticides on beneficial insects and soil health, the importance of waiting periods, safe pesticide disposal, and safety precautions. Significant associations were found between IPM training and chemical pesticide use, awareness of their impacts on beneficial insects, pesticide disposal methods, waiting periods, safety precautions, and perceptions of soil impact. The study highlighted the critical role of training in enhancing pest management practices and awareness of the adverse effects of chemical pesticides, underscoring the need for increased availability of biopesticides and support for IPM adoption
Molecular identification of biosurfactant-producing bacteria obtained from oil-polluted soil samples in Abakaliki, Ebonyi State, Nigeria
No full textSeveral human activities have led to environmental pollution with crude oil and other related hydrocarbons. There are various technologies of remediating oil-polluted environments, but the use of biological agents such as biosurfactants are widely practiced, and are generally considered as safe and eco-friendly. This study aimed at isolating and identifying biosurfactant-producing bacteria obtained from oil polluted soil samples in Abakaliki, Ebonyi State, Nigeria using molecular techniques. Six waste oil-contaminated soil samples were collected from mechanic and generator repair workshops, and were analyzed using standard microbiological methods. Nine bacterial isolates were obtained from the waste oil-contaminated soil samples, and were suspected to be Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus. Molecular identification of the biosurfactant-producing isolates was also carried out using PCR and the genes responsible for biosurfactant production targeted. Oil spreading technique was used to detect the oil displacement activity (ODA) of the isolates. The emulsification index (E24) was calculated and the ability of the extracted biosurfactant to emulsify the hydrocarbons was determined. Pseudomonas aeruginosa had the highest ODA of 28.1 cm while Staphylococcus aureus had the least ODA of 5 cm. Pseudomonas aeruginosa showed E24 of 70.1%, Bacillus subtilis 57.1% while S. aureus showed 32.5%. Hence, Pseudomonas and Bacillus species have good potentials for biosurfactant production, and can be harnessed and utilized in the cleanup of oil polluted soil
A review on seed priming to combat climate variability in agriculture
No full textGlobal agriculture faces immense challenges due to climate change, which causes unpredictable weather patterns, decreased agricultural productivity, and decreased food security. Seed priming is critical in combating climate variability because it has emerged as a promising method for improving seed germination and agricultural resilience. This review evaluates the efficiency of several seed priming techniques, including hydro-priming, halo-priming, osmo-priming, bio-priming, chemical priming, and hormone priming. These techniques improve seedling vigor, stress tolerance, and overall crop yield. Seed priming increases germination rates and resistance to biotic and abiotic stresses, such as salinity and drought, while improving agricultural output and disease resistance. Seed priming reduces the demand for chemical pesticides and fertilizers by increasing soil quality and nutrient absorption, which supports sustainable agriculture. This review highlights the potential benefits of seed priming as a practical, affordable, and practical strategy to reduce the negative effects of climatic variability on agriculture. Future studies should focus on developing the best priming techniques for diverse crop varieties and conditions, as well as examining the combined impacts of various priming strategies. Seed priming will be crucial to preserving food security and agricultural sustainability in the face of ongoing climate change
Screening of blackgram (Vigna mungo) genotypes for resistance to cercospora leaf spot and yield performance in Nepal
No full textThis study screened 12 black gram (Vigna mungo L.) genotypes for resistance to Cercospora leaf spot (CLS) caused by Cercospora canescens. Conducted at the research plot of the Mid-West Academy and Research Institute, Tulsipur, Dang, the experiment utilized a randomized complete block design with three replications from September 2022 to January 2023. Disease severity was evaluated at 40, 47, and 54 days after sowing (DAS), with scoring based on the percentage of infected leaf area at 7-day intervals. Key disease metrics—incidence, severity, mean area under the disease progress curve (AUDPC), and yield—were calculated. Results indicated no significant difference in CLS incidence among genotypes. However, significant differences in disease severity were noted at 40, 47, and 54 DAS, with AUDPC values differing across genotypes. Based on mean AUDPC values, 10 genotypes were categorized as moderately resistant, while BLG 0066-1-1 and BLG 0035-1 were moderately susceptible. BLG 0035-1 had the highest AUDPC (324.1), and BLG 0069-1 had the lowest (175). Yield varied significantly, with the highest yield (799 kg/ha) recorded for BLG 0068-2 and the lowest (495 kg/ha) for BLG 0066-1. These findings support breeding efforts targeting CLS resistance and enhanced yield in black gram