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Exploring Nelson Mandela Bay’s coastal and marine tourism potential
Get PDFThis study investigates some of the gaps in Coastal and Marine Tourism (CMT) within the Nelson Mandela Bay Municipality (NMBM), South Africa. The NMBM boasts one of the most biologically diverse coastlines. Therefore, CMT's growth and development opportunities are unparalleled, but some challenges inhibit the sector from realizing this potential. The study aims to identify these challenges and explore opportunities to strengthen CMT for sustainable economic and environmental benefits. To achieve this, the study employs a qualitative approach, specifically using semi-structured interviews conducted with a wide array of stakeholders, including tourism officials, tourists, business owners in the locality, environmentalists, and government representatives. This is further complemented by document analysis of local and international documents, providing historical and contextual insights into tourism development. A total of 15 interviews were conducted and analysed. This is further complemented by document analysis of local and international policy papers and marketing materials, providing historical and contextual insights into tourism development. The analysis includes both local (South African) and international documents. Of the 26 documents, 8 are local and 18 are international. The predominance of international sources is due to the limited local literature on CMT directly related to the research topic and objectives. International documents provide valuable case studies and best practices that can inform sustainable CMT development in NMBM by drawing from successful global models while aligning them with local needs. Key findings identify several factors constraining CMT, including socioeconomic challenges, environmental management issues, and limited infrastructure and marketing support. However, stakeholders highlight significant opportunities for sustainable CMT development through increased investment and community engagement. Based on these insights, the study proffers focused strategies aimed at diversifying and strengthening CMT within the NMBM to achieve economic growth while ensuring environmental protection. Recommendations include improving infrastructure, enhancing marketing efforts, and fostering collaboration between stakeholders to maximize the potential of NMBM’s coastal and marine assets. By addressing these limitations, this study contributes to a sustainable tourism framework that can be adapted to similar regions.Thesis (MMM) -- Faculty of Business and Economic Sciences, School of Economics, Development and Tourism, 202
Characterization of the molecular mechanism(s) of cannabinoid-induced paraptosis in breast cancer cells
Get PDFNatural products have been extensively studied for their anticancer potential, and several widely used anticancer drugs have natural origins. Many studies have demonstrated that natural compounds can induce paraptosis in various tumour cell lines. Paraptosis is a caspase-independent cell death mechanism characterised by cytoplasmic vacuolation arising from the endoplasmic reticulum (ER) and the mitochondria. The molecular mechanism of paraptosis is unclear; however, the literature indicates that dysregulation of calcium signalling plays an important role in paraptosis induction, particularly calcium-mediated signalling between the ER and mitochondria. This study aimed to identify and characterise the mechanism of cell death induced by a phytocannabinoid ratio which induced significant cytoplasmic vacuolation in the MCF7 breast cancer cell line. Several techniques were employed to address the aim of the study. The crystal violet assay was used to detect changes in viability. Several pathway inhibitors, as well as fluorescent staining and analysis using high-content screening (HCS), were used to measure the induction of various cell death mechanisms. Morphological changes were investigated using light and transmission electron microscopy. The phytocannabinoid ratio induced significant cell death and cytoplasmic vacuolation in MCF7 cells; however, the same trend was not observed in the MCF10A non-tumourigenic breast cell line. No cell cycle arrest, apoptosis, necrosis, autophagy, or ferroptosis induction was detected in MCF7 cells, suggesting that an alternative mechanism of cell death was induced. Vacuolation and cell death induced by the phytocannabinoid ratio were inhibited by cycloheximide, suggesting a dependence on protein synthesis, which is characteristic of paraptosis induction. The mechanism of paraptosis induction by the phytocannabinoid ratio was investigated, and it was found that treatment 1) induced ER dilation and mitochondrial swelling; 2) induced significant ER stress, mitochondrial calcium overload, and mitochondrial dysfunction which appeared to be mediated by the voltage-dependent anion channel (VDAC); and 3) significantly impaired all mitochondrial metabolic pathways. Overall, the data demonstrated that paraptosis induced by the cannabinoid ratio is mediated by calcium flux from the ER to the mitochondria. This highlighted a novel mechanism of phytocannabinoid-induced cell death and emphasised the anti-cancer potential of the use of phytocannabinoid ratios as opposed to individual phytocannabinoids, expanding their potential for use as anticancer agents.Thesis (PhD) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 202
The review of a disciplinary finding or sanction in terms of section 158(1)(h) of the labour relations act 66 of 1995
Get PDFWhen an employer subjects an employee to a formal disciplinary process, the employer appoints a presiding officer to whom it cedes either wholly or partly its decision-making powers. Wholly in that the presiding officer would render a finding and impose a sanction. Partly, in that the presiding officer would render a finding and only provide the employer with a recommendation on sanction, which the employer would either accept or reject. The State in its capacity as employer has been afforded the ability to utilise section 158(1)(h) of the Labour Relations Act, 1995 (LRA) to review the decisions of presiding officers appointed over internal disciplinary proceedings. Despite the wording of section 158(1)(h), the courts have restricted its use by employees employed by the State. The courts have held that a State employee would have to utilise the ordinary dispute resolution pathways made available in the LRA to challenge the decisions the State takes in its capacity as an employer. When exercising the prerogative to discipline, all employers, including the State, must primarily comply with the substantive and procedural requirements outlined in Schedule 8 of the LRA. Notwithstanding the primary measure of fairness, the employer’s decision to discipline and dismiss would also be pitched and measured against its adopted internal disciplinary code and procedure document. It has been questioned in a Labour Court judgement whether the State should continue to be afforded the benefit of the section 158(1)(h) review. The Labour Court judgement ventures into the consequences the use of section 158(1)(h) would have on the unfair dismissal dispute resolution pathway afforded by the LRA and proposed that it would be ideal for the State to utilise alternatives when attempting to alter the sanction or finding of its internal presiding officers. The alternatives proposed include the internal review of findings or sanctions, the unilateral substitution of sanctions, increasing sanctions on appeal and the practice of subjecting the employee to a second hearing. Applying the alternatives to the current circumstances and collective bargaining agreements applicable in the public service, the State would not be able to utilise the alternatives, save for subjecting the employee to a second hearing in exceptional circumstances. In all other instances, including convening an internal review, increasing a sanction during an appeal, or unilaterally substituting the sanction would conflict with the prevailing internal disciplinary code and procedure in the public service. In as much as the Labour Court judgment attempts to put into perspective the consequences the use of section 158(1)(h) would have on the subsequent dispute resolution pathways, the State should not be unduly bound by irrational findings or sanctions imposed by presiding officers appointed to preside over its internal disciplinary hearings.Thesis (LLM) -- Faculty of Law, School of Mercantile Law, 202
The inhibitory effects of cannabinoids from cannabis sativa on the enzymes dipeptidyl peptidase-IV, sucrase and maltase as a new therapeutic treatment for type 2 diabetes
Get PDFType 2 diabetes is one of the most prevalent diseases worldwide. The treatments used to manage diabetes often have severe side effects and patients develop resistance to traditional treatment. The project aimed to test if phytocannabinoids from Cannabis sativa inhibited key enzymes involved in glycaemic homeostatic regulation, namely dipeptidyl peptidase 4 (DPP-4), sucrase, and maltase. This study investigated the inhibitory effects of 3 M-128 M cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG), and Δ9- tetrahydrocannabinol (THC). CD spectroscopy was used to investigate the changes in the secondary structure of DPP-4 with interacting inhibitors. The effect of 1.25, 2.5, and 5 mg/kg rat THC cannabis extract on the activity of DPP-4 in blood plasma and rat pancreatic tissue of the diabetic rat model and obese rat model. The effect of 1.25, 2.5, and 5 mg/kg rat THC cannabis extract on glucagon concentration in the blood plasma of the diabetic rat model and obese rat model was investigated. The carbohydrate digestive enzymes namely -amylase, -glucosidase and maltase are not inhibited by any of the cannabinoids. CBN had inhibitory effects on sucrase. CBN, CBG, and CBD are mixed inhibitors of DPP-4, thus they can inhibit DPP-4 competitively and uncompetitively depending on the concentration of the cannabinoid. THC was shown in kinetic and rat model studies to be a very weak inhibitor of DPP-4. CD spectroscopy showed that sitagliptin (FDA-approved drug and competitive inhibitor) and CBG mimic the denatured structure of DPP-4. CBD, CBN and THC mimic the free (active) form of DPP-4. A reduction in pancreatic DPP-4 activity was observed with 2.5 and 5 mg/kg rat THC (diabetic model). This study showed that diet plays a role in glycaemic dysregulation (obese rat model) and that insulin-resistant rats had four times higher glucagon levels compared to the lean control (diabetic model). 1.25 mg/kg rat THC reduced blood plasma DPP-4 activity and blood plasma glucagon. Cannabis sativa can be a feasible treatment to help manage type 2 diabetes by inhibiting DPP-4, especially medical strains of Cannabis sativa with high concentrations of CBD and CBG.Thesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 202
Supramolecular chemistry considerations of fluorenyl-derived host compounds
Get PDFThis work examined various supramolecular aspects of 9-fluorenone-derived host compounds, namely 4,4’-(9-fluorenylidene)diphenol (H1), 9,9-(1,4-phenylene)bis(fluoren-9-ol) (H2), 9,9- (ethyne-1,2-diyl)bis(fluoren-9-ol) (H3) and 9,9-(biphenyl-4,4-diyl)bis(fluoren-9-ol) (H4). These compounds were successfully synthesized and then screened for their host ability for, and host behaviour in, various organic compounds. At the outset, molecular modelling calculations were carried out on each of the four host compounds. Various structural parameters and the geometries of the low energy conformers that were calculated were then compared with these aspects of the guest-free host molecules obtained from single crystal X-ray diffraction (SCXRD) analysis. The host behaviour of H1 was, furthermore, assessed in the presence of four alkylnitriles, namely acetonitrile (ACE), acrylonitrile (ACRY), propionitrile (PROP) and butyronitrile (BUT). All four guests complexed with H1 with 1:1 host:guest (H:G) ratios. From SCXRD analyses, each of the complexes crystallized in the monoclinic crystal system and the centrosymmetric space group P21/n, and the host packing was isostructural in each instance. The complexes were stabilized by a plethora of short inter- and intramolecular contacts. The thermal stabilities of the four complexes were also examined together with Hirshfeld surface considerations. Finally, lattice energy calculations were carried out and the results of these compared with the relative thermal stabilities of the four complexes. The compounds H2 and H3 were examined for their selectivity behaviour in mixtures of aniline, N-methylaniline and N,N’-dimethylaniline (ANI, NMA and DMA). Both host compounds were able to form complexes with ANI and DMA with various H:G ratios, while only H3 possessed enclathration ability for NMA (H:G 1:1). SCXRD analyses showed that all of the complexes were stabilized by means of classical (host)O‒H···N(guest) hydrogen bonds in addition to other short contacts. Equimolar binary guest competition experiments with H2 revealed an extremely high host selectivity for DMA (ANI/DMA and DMA/NMA experiments furnished crystals with > 91% DMA), while the experiment with all three anilines present also resulted in a complex with anThesis (MSc) -- Faculty of Science, School of Biomolecular & Chemical Sciences, 202
Strategies for amplifying street vendors’ contribution to local economic development in Limpopo: a case study of vendors in Thohoyandou Plaza
Get PDFThe study investigated strategies for amplifying street vendors' contribution to local economic development at Thohoyandou Plaza in Limpopo Province. Street vendors have been acknowledged as a critical component of global and South African socioeconomic growth. Nonetheless, very little study has been undertaken in Thohoyandou Plaza with the primary goal of identifying the problems and potential for increasing the number of street vendors. Consequently, this study adds to current knowledge by focusing on approaches for raising the number of street vendors in the area. Access to finance, infrastructure, laws and licensure were among the barriers noted in this study. The sample comprised 23 participants selected through purposive sampling techniques. The study population included street vendors and municipal officers. The study interviewed 18 vendors and 5 government officials who met the criteria and were willing to participate. A literature review was conducted and found that the constraints include partial access to storage services, which explains the inadequate options for street vendors to succeed. The study used qualitative research methodology to attain intended goals. Furthermore, the study used an interview guide to obtain data from individual participants using a face-to-face method. To acquire information from important informants, open and closed-ended questionnaires were also used. Recruitment was done to street vendors who showed interest in contributing to the study. Data were collected through questionnaires and interview schedules. Atlas ti. Version 23 was used in coding and categorising the data according to emerging themes for qualitative data. Views and perspectives of various individuals and previous studies were considered to give a tangible outcome, conclusion and recommendations. Street vendors have challenges such as poor literacy and limited access to financial assistance. Therefore, training on financial literacy, marketing and managerial abilities is required for them to improve their market opportunities. Private organisations should invest in the infrastructure required for the success of street vendors. This will in turn help street vendors to contribute significantly to local economic development while also alleviating unemployment. The recommendation of the current study aimed to advance the working conditions of street vendors, particularly those selling fruits and vegetables. The study's outcomes may aid street vendors by decreasing the obstacles connected with street trade. The findings of the study can support policymakers to address relevant restrictions faced by these street vendors by making it easier to access critical raw materials, which might be challenging owing to financial and seasonal concerns.Thesis (MA) -- Faculty of Business and Economic Sciences, School of Economics, Development and Tourism, 202
Factors influencing consumer buying behaviour of luxury goods among the LGBTQI+ market segment
Get PDFThis study delves into the factors shaping the purchasing behaviour of luxury goods within the LGBTQI+ market segment in South Africa. Despite being a substantial and diverse market with distinctive consumer preferences, limited research exists to understand the specific motivations driving the LGBTQI+ community's luxury product purchases. The growing acceptance of the LGBTQI+ community has not only increased their visibility but has also led to heightened consumer spending within this segment. The Pink Economy, a term coined to describe the economic power of the LGBTQI+ community, highlights their substantial financial contributions across various sectors. This encompasses businesses and industries customized specifically for LGBTQI+ consumers, ranging from essential goods to luxury items like those found in fashion, travel, and entertainment. Against the backdrop of the expanding luxury brand product category and the increasing expenditures of the LGBTQI+ market segment, particularly in the luxury sector, there arises a need to explore strategies for attracting a more significant share of LGBTQI+ consumers. Diverse motivations drive the purchase of luxury goods within this segment, with individuals from various demographics having distinct perspectives on and connections to luxury brands. To investigate these factors, the study targeted individuals who identify as queer or non-heterosexual. Specifically, those who identify as lesbian, gay, bisexual, transgender, queer, intersex, and other sexual orientations such as pansexual and Two-Spirit (LGBTQI+), reside within South Africa and were between the ages of 18 and 65. The Taro Yamane formula was used to determine the sample size needed for the study. It identified a required sample size of 400 respondents, and the study ultimately received 428 responses. The data was analyzed using Statistical Package for the Social Sciences (SPSS) 28, and Structural Equation Modelling (SEM) using Analysis of Moment Structures (AMOS) 28, aimed to reveal insights into the relationships between variables. The findings highlight a significant and positive correlation between psychological factorsThesis (MCom) -- Faculty of Business and Economic Sciences, School of Management Sciences, 202
Relationship management in all-boys fee-paying public Schools in the Eastern Cape, South Africa: experiences and perspectives of LGBTQ alumni
Get PDFThis study explores the perspectives and experiences of LGBTQ alumni of all-boys’ schools in the Eastern Cape. The context of increased competition between schools in the education sector and the importance of inclusion within the sector necessitated a study to investigate the extent to which relationship management is conducted with an orientation towards LGBTQ inclusion in all-boys’ schools in the Eastern Cape. Student protests in South Africa over the past 10 years have expanded concerns about student belonging beyond the widely studied lenses of race and class. A growing body of literature and wider discourse emphasises student belonging as a challenge in the South African education sector. Marginalised students continue to experience educational spaces negatively. LGBTQ student experiences in the South Africa are characterised by violence and ostracisation, thus warranting further investigation and inquiry. The study locates itself in the intersections between gender and sexual diversity and economic citizenship in the broader discourse of school inclusivity. A qualitative research approach was employed, making use of semi-structured, in-depth individual interviews. The sample was derived from alumni of all-boys’ schools in the Eastern Cape through snowball sampling. Ten LGBTQ-identifying alumni of boys’ schools in the Eastern Cape were interviewed. The findings reveal the need to question the extent to which LGBTQ alumni can enjoy the fullness of the value proposition of all-boys’ schools, and what can be done to ensure LGBTQ belonging. The findings of this study concur with existing literature about LGBTQ experiences. Participants defined their school experiences as exclusionary and characterised by fear of failing to assert themselves in a hypermasculine school environment. LGBTQ alumni of all-boys’ schools in the Eastern Cape view relationship management as under-utilised as a tool to facilitate LGBTQ belonging. Instead, relationship management is used to affirm those who fit the mould of the quintessential ‘old boy’ network, by defining the masculine behaviours that learners in all-boys’ schools are compelled to aspire towards.Thesis (MCom) -- Faculty of Business and Economic Sciences, School of Management Sciences, 202
Supporting competitive robot game mission planning using machine learning
Get PDFThis dissertation presents a study aimed at supporting the strategic planning and execution of missions in competitive robot games, particularly in the FIRST LEGO® League (FLL), through the use of machine learning techniques. The primary objective is to formulate guidelines for evaluating mission strategies using machine learning techniques within the FLL landscape, thereby supporting participants in the mission strategy design journey within the FLL robot game. The research methodology encompasses a literature review, focusing on the current practices in the FLL mission strategy design process. This is followed by a literature review of machine learning techniques on a broad level pivoting towards evolutionary algorithms. The study then delves into the specifics of genetic algorithms, exploring their suitability and potential advantages for mission strategy evaluation in competitive robotic environments within the FLL robot game. A significant portion of the research involves the development and testing of a prototype system that applies a genetic algorithm to simulate and evaluate different mission strategies, providing a practical tool for FLL teams. During the development of the evaluation prototype, guidelines were formulated aligning with the primary research objective which is to formulate guidelines for evaluating mission strategies in robot games using machine learning techniques. Key findings of this study highlight the effectiveness of genetic algorithms in identifying optimal mission strategies. The prototype demonstrates the feasibility of using machine learning to provide real-time, feedback to participating teams, enabling more informed decision-making in the formulation of mission strategies.Thesis (MIT) -- Faculty of Engineering, the Built Environment, and Technology, School of Information Technology, 202
A discourse analysis study of traditional health practitioners’ role in the treatment of female sexual violence and its mental health sequelae in KwaZulu-Natal
Get PDFSouth Africa is considered the epicentre of rape, leading to its reputation as the ‘rape capital of the world’. As a way of mitigating the risk of re-victimisation to individuals who have experienced rape, hospital-based post-rape centres were developed to offer services and support to rape victims. The uptake of these services remains scant as issues such as rape stigma, limited knowledge on their availability and alternative health systems limits their usage. In South Africa, there exists medical pluralism, which refers to multiple ways of making sense of illness and treatment seeking methods. It is estimated that around 80% of South Africans utilise the services of traditional health practitioners in conjunction with biomedical health practices or as a standalone service to get alternative solutions for their health needs. Victims of rape need to access a wide range of services such as post-exposure prophylaxis (PEP), medical examinations, and emergency contraception. There has been a paucity in research looking into the alternative treatments accessed by rape victims who do not access services in the formal healthcare system. It is against this backdrop that this study explored the construction and treatment formulation of rape by traditional health practitioners. The study focused on the use of language and how it becomes action that influences the services rendered to rape victims. Thematic analysis, discourse and conversational analytical approaches were used to analyse data that emerges from the one-on-one interviews. 15 traditional healers who identified as abathandazi, Izangoma and izinyanga were selected to participate in the present study. The healthworlds framework and social constructionism were used to make sense of how traditional health practitioners construct rape and how the dominant discourses surrounding rape influence how they understand their position as healthcare providers. The main findings of the study illustrated that in the healthworld of traditional healers’ understanding of rape was constructed as ukungcola nobumnyama / impurity and darkness. These discourses spoke to the perception of rape as an aberration as one’s ancestors should prevent traumas and catastrophes from befalling on the individual. When rape occurs, it signalled spiritual problems resulting into a phenomenon where ancestors cannot safeguard the living. Under these discourse rape was also constructed as something that taints, impurifies and contaminates not just the raped individual but their entire bloodline and ancestors. As a result of the discourse surrounding rape, cleansing and purification rituals played a huge role in post-rape care. In addition, traditional health practitioners viewed their role as facilitators in reestablishing the relationship between the raped individual and their ancestors. The study observed that rape victims who share the same healthworld are likely to experience spiritual transgressions following rape which will have long-lasting effects on the psychological health outcomes of victims. The present study recommends a post-rape care model that advocates for collaborative opportunities between biomedical and traditional healthcare practitioners. This model can be integrated into existing post-rape care systems to provide holistic and comprehensive healthcare that addresses biopsychosocial as well as the spiritual and cultural influences of rape.Thesis (DPhil) -- Faculty of Health Science, School of Behavioural & Lifestyle Sciences, 202